{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:57:36Z","timestamp":1778817456610,"version":"3.51.4"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004573","name":"Ministry of Agriculture of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004573","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008562","name":"Nanjing Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008562","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008868","name":"Jiangsu Science and Technology Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1016\/j.virusres.2020.197912","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T13:04:38Z","timestamp":1583240678000},"page":"197912","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies"],"prefix":"10.1016","volume":"281","author":[{"given":"Xianwei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.virusres.2020.197912_bib0005","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s00705-009-0547-x","article-title":"Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009)","volume":"155","author":"Carstens","year":"2010","journal-title":"Arch. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0010","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S1016-8478(23)15106-5","article-title":"Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus","volume":"14","author":"Chang","year":"2002","journal-title":"Mol. Cells"},{"key":"10.1016\/j.virusres.2020.197912_bib0015","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1007\/s00705-010-0720-2","article-title":"Molecular epidemiology of porcine epidemic diarrhea virus in China","volume":"155","author":"Chen","year":"2010","journal-title":"Arch. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0020","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.virusres.2016.09.003","article-title":"From the field to the lab - an European view on the global spread of PEDV","volume":"226","author":"Choudhury","year":"2016","journal-title":"Virus Res."},{"key":"10.1016\/j.virusres.2020.197912_bib0025","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.virusres.2007.10.015","article-title":"The GPRLQPY motif located at the carboxy-terminal of the spike protein induces antibodies that neutralize Porcine epidemic diarrhea virus","volume":"132","author":"Cruz","year":"2008","journal-title":"Virus Res."},{"key":"10.1016\/j.virusres.2020.197912_bib0030","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/978-1-4615-2996-5_9","article-title":"Genome organization of porcine epidemic diarrhoea virus","volume":"342","author":"Duarte","year":"1993","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.virusres.2020.197912_bib0035","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1099\/jgv.0.000586","article-title":"Immunogenicity of a recombinant parapoxvirus expressing the spike protein of Porcine epidemic diarrhea virus","volume":"97","author":"Hain","year":"2016","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0040","doi-asserted-by":"crossref","first-page":"e713","DOI":"10.1128\/mBio.00737-13","article-title":"Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States","volume":"4","author":"Huang","year":"2013","journal-title":"MBIO"},{"key":"10.1016\/j.virusres.2020.197912_bib0045","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.tvjl.2015.02.017","article-title":"Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis","volume":"204","author":"Jung","year":"2015","journal-title":"Vet. J."},{"key":"10.1016\/j.virusres.2020.197912_bib0050","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0378-1135(92)90100-8","article-title":"An ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV) based on the specific solubility of the viral surface glycoprotein","volume":"32","author":"Knuchel","year":"1992","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0055","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1023\/A:1011831902219","article-title":"Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence","volume":"23","author":"Kocherhans","year":"2001","journal-title":"Virus Genes"},{"key":"10.1016\/j.virusres.2020.197912_bib0060","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1023\/A:1023447732567","article-title":"Cloning and sequence analysis of the nucleocapsid gene of porcine epidemic diarrhea virus Chinju99","volume":"26","author":"Lee","year":"2003","journal-title":"Virus Genes"},{"key":"10.1016\/j.virusres.2020.197912_bib0065","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.3201\/eid1803.120002","article-title":"New variants of porcine epidemic diarrhea virus, China, 2011","volume":"18","author":"Li","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.virusres.2020.197912_bib0070","article-title":"Cell attachment domains of the PEDV spike protein are key targets of neutralizing antibodies","author":"Li","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0075","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/JVI.03196-14","article-title":"Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains","volume":"89","author":"Lin","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0080","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.virusres.2016.05.023","article-title":"Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains","volume":"226","author":"Lin","year":"2016","journal-title":"Virus Res."},{"key":"10.1016\/j.virusres.2020.197912_bib0085","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01744-16","article-title":"Complete genome sequence of variant porcine epidemic diarrhea virus strain CH\/HNZZ47\/2016 isolated from suckling piglets in China","volume":"5","author":"Liu","year":"2017","journal-title":"Genome Announc."},{"key":"10.1016\/j.virusres.2020.197912_bib0090","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.vetmic.2016.02.004","article-title":"Two-way antigenic cross-reactivity between porcine epidemic diarrhea virus and porcine deltacoronavirus","volume":"186","author":"Ma","year":"2016","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0095","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1099\/0022-1317-39-3-545","article-title":"Ribonucleoprotein-like structures from coronavirus particles","volume":"39","author":"Macneughton","year":"1978","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0100","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.1007\/s00705-014-2163-7","article-title":"Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein","volume":"159","author":"Oh","year":"2014","journal-title":"Arch. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0105","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0962-8924(00)01738-4","article-title":"The nucleolus: an old factory with unexpected capabilities","volume":"10","author":"Olson","year":"2000","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.virusres.2020.197912_bib0110","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s00705-010-0892-9","article-title":"Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea","volume":"156","author":"Park","year":"2011","journal-title":"Arch. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0115","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/BF01317606","article-title":"A new coronavirus-like particle associated with diarrhea in swine","volume":"58","author":"Pensaert","year":"1978","journal-title":"Arch. Virol."},{"key":"10.1016\/j.virusres.2020.197912_bib0120","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.epidem.2015.11.002","article-title":"Decision-making for foot-and-mouth disease control: objectives matter","volume":"15","author":"Probert","year":"2016","journal-title":"Epidemicsneth"},{"key":"10.1016\/j.virusres.2020.197912_bib0125","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0378-1135(93)90030-B","article-title":"Coronavirus immunogens","volume":"37","author":"Saif","year":"1993","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0130","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.3390\/v6031253","article-title":"Molecular characterizations of subcellular localization signals in the nucleocapsid protein of porcine epidemic diarrhea virus","volume":"6","author":"Shi","year":"2014","journal-title":"Viruses"},{"key":"10.1016\/j.virusres.2020.197912_bib0135","doi-asserted-by":"crossref","first-page":"39700","DOI":"10.1038\/srep39700","article-title":"Nucleocapsid interacts with NPM1 and protects it from proteolytic cleavage, enhancing cell survival, and is involved in PEDV growth","volume":"7","author":"Shi","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.virusres.2020.197912_bib0140","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0378-1135(99)00199-6","article-title":"Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages","volume":"72","author":"Shibata","year":"2000","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0145","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s11262-012-0713-1","article-title":"Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines","volume":"44","author":"Song","year":"2012","journal-title":"Virus Genes"},{"key":"10.1016\/j.virusres.2020.197912_bib0150","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0065-3527(08)60721-6","article-title":"The molecular biology of coronaviruses","volume":"28","author":"Sturman","year":"1983","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.virusres.2020.197912_bib0155","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.vetmic.2008.02.022","article-title":"Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein","volume":"131","author":"Sun","year":"2008","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0160","doi-asserted-by":"crossref","first-page":"161","DOI":"10.3201\/eid1801.111259","article-title":"Outbreak of porcine epidemic diarrhea in suckling piglets, China","volume":"18","author":"Sun","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.virusres.2020.197912_bib0165","doi-asserted-by":"crossref","DOI":"10.3390\/v10090467","article-title":"S1 subunit of spike protein from a current highly virulent porcine epidemic diarrhea virus is an important determinant of virulence in piglets","volume":"10","author":"Suzuki","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.virusres.2020.197912_bib0170","doi-asserted-by":"crossref","first-page":"39010","DOI":"10.1038\/srep39010","article-title":"The identification and characterization of two novel epitopes on the nucleocapsid protein of the porcine epidemic diarrhea virus","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.virusres.2020.197912_bib0175","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1006\/viro.1999.0025","article-title":"The nucleocapsid protein of coronavirus mouse hepatitis virus interacts with the cellular heterogeneous nuclear ribonucleoprotein A1 in vitro and in vivo","volume":"265","author":"Wang","year":"1999","journal-title":"Virology"},{"key":"10.1016\/j.virusres.2020.197912_bib0180","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1136\/vr.100.12.243","article-title":"An apparently new syndrome of porcine epidemic diarrhoea","volume":"100","author":"Wood","year":"1977","journal-title":"Vet. Rec."},{"key":"10.1016\/j.virusres.2020.197912_bib0185","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.vetmic.2013.01.034","article-title":"Porcine epidemic diarrhea virus N protein prolongs S-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression","volume":"164","author":"Xu","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0190","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s11262-012-0867-x","article-title":"Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011","volume":"46","author":"Yang","year":"2013","journal-title":"Virus Genes"},{"key":"10.1016\/j.virusres.2020.197912_bib0195","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1089\/hyb.2011.0028","article-title":"SARS coronavirus nucleocapsid protein monoclonal antibodies developed using a prokaryotic expressed protein","volume":"30","author":"Zhang","year":"2011","journal-title":"Hybridoma (Larchmt)"},{"key":"10.1016\/j.virusres.2020.197912_bib0200","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1186\/1743-422X-9-225","article-title":"Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus","volume":"9","author":"Zhang","year":"2012","journal-title":"Virol. J."},{"key":"10.1016\/j.virusres.2020.197912_bib0205","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.vetmic.2019.01.003","article-title":"Lactobacillus acidophilus S-layer protein-mediated inhibition of PEDV-induced apoptosis of Vero cells","volume":"229","author":"Zhang","year":"2019","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2020.197912_bib0210","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jviromet.2016.01.011","article-title":"Baculovirus expression of the N-terminus of porcine heat shock protein Gp96 improves the immunogenicity of recombinant PCV2 capsid protein","volume":"230","author":"Zhu","year":"2016","journal-title":"J. Virol. Methods"}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170219306677?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170219306677?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:08:48Z","timestamp":1759154928000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170219306677"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":42,"alternative-id":["S0168170219306677"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2020.197912","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2020.197912","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"197912"}}