{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T23:24:02Z","timestamp":1777073042284,"version":"3.51.4"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100007162","name":"Guangdong Science and Technology Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1016\/j.virusres.2020.197975","type":"journal-article","created":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T22:02:58Z","timestamp":1587160978000},"page":"197975","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Development of the reverse genetics system for emerging atypical porcine pestivirus using in vitro and intracellular transcription systems"],"prefix":"10.1016","volume":"283","author":[{"given":"Jianxin","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xujiao","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huizi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianglong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingqian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhangyong","family":"Ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.virusres.2020.197975_bib0005","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.antiviral.2014.12.007","article-title":"Flavivirus reverse genetic systems, construction techniques and applications: a historical perspective","volume":"114","author":"Aubry","year":"2015","journal-title":"Antiviral. Res"},{"issue":"5","key":"10.1016\/j.virusres.2020.197975_bib0010","doi-asserted-by":"crossref","first-page":"e22","DOI":"10.1111\/tbed.12532","article-title":"High Prevalence of Highly Variable Atypical Porcine Pestiviruses Found in Germany","volume":"64","author":"Beer","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"issue":"4","key":"10.1016\/j.virusres.2020.197975_bib0015","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/lam.12834","article-title":"Establishment and evaluation of an indirect immunofluorescence assay for the detection of salmonid alphavirus","volume":"66","author":"Gao","year":"2018","journal-title":"Lett. Appl. Microbiol."},{"issue":"2","key":"10.1016\/j.virusres.2020.197975_bib0020","doi-asserted-by":"crossref","first-page":"e339","DOI":"10.1111\/tbed.12759","article-title":"Detection of atypical porcine pestivirus in semen from commercial boar studs in the United States","volume":"65","author":"Gatto","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"issue":"4","key":"10.1016\/j.virusres.2020.197975_bib0025","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1354\/vp.43-4-530","article-title":"Neuropathologic study of experimental classical swine fever","volume":"43","author":"Gomez-Villamandos","year":"2006","journal-title":"Vet. Pathol"},{"issue":"10","key":"10.1016\/j.virusres.2020.197975_bib0030","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.1099\/jgv.0.000251","article-title":"Discovery of a novel putative atypical porcine pestivirus in pigs in the USA","volume":"96","author":"Hause","year":"2015","journal-title":"J. Gen. Virol."},{"issue":"9","key":"10.1016\/j.virusres.2020.197975_bib0035","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1073\/pnas.1618424114","article-title":"Entirely plasmid-based reverse genetics system for rotaviruses","volume":"114","author":"Kanai","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"10.1016\/j.virusres.2020.197975_bib0040","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1007\/s00705-012-1548-8","article-title":"Efficient and stable rescue of classical swine fever virus from cloned cDNA using an RNA polymerase II system","volume":"158","author":"Li","year":"2013","journal-title":"Arch. Virol."},{"key":"10.1016\/j.virusres.2020.197975_bib0045","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.virusres.2015.12.022","article-title":"Generation of a reliable full-length cDNA of infectious Tembusu virus using a PCR-based protocol","volume":"213","author":"Liang","year":"2016","journal-title":"Virus Res."},{"key":"10.1016\/j.virusres.2020.197975_bib0050","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1007\/s00705-019-04345-3","article-title":"Viral load and histological distribution of atypical porcine pestivirus in different tissues of naturally infected piglets","volume":"164","author":"Liu","year":"2019","journal-title":"Arch. Virol."},{"issue":"3","key":"10.1016\/j.virusres.2020.197975_bib0055","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/tbed.12839","article-title":"Genetic and phylogenetic analysis of reemerged novel Seneca Valley virus strains in Guangdong province, 2017","volume":"65","author":"Liu","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.virusres.2020.197975_bib0060","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.virol.2018.08.018","article-title":"Homologous RNA secondary structure duplications in 3\u2019 untranslated region influence subgenomic RNA production and replication of dengue virus","volume":"524","author":"Liu","year":"2018","journal-title":"Virology"},{"issue":"1","key":"10.1016\/j.virusres.2020.197975_bib0065","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/tbed.12753","article-title":"Presence of atypical porcine pestivirus (APPV) in Brazilian pigs","volume":"65","author":"Mosena","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"issue":"1","key":"10.1016\/j.virusres.2020.197975_bib0070","doi-asserted-by":"crossref","first-page":"e2018","DOI":"10.1002\/rmv.2018","article-title":"An emerging novel virus: Atypical porcine pestivirus (APPV)","volume":"29","author":"Pan","year":"2019","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.virusres.2020.197975_bib0075","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jviromet.2015.09.008","article-title":"Development and characterization of reverse genetics system for the Indian West Nile virus lineage 1 strain 68856","volume":"226","author":"Pavitrakar","year":"2015","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.virusres.2020.197975_bib0080","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jviromet.2017.07.008","article-title":"A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA viruses from cloned full-length cDNA","volume":"248","author":"Peeters","year":"2017","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.virusres.2020.197975_bib0085","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.virol.2018.04.001","article-title":"Phosphorodiamidate morpholino targeting the 5\u2019 untranslated region of the ZIKV RNA inhibits virus replication","volume":"519","author":"Popik","year":"2018","journal-title":"Virology"},{"key":"10.1016\/j.virusres.2020.197975_bib0090","doi-asserted-by":"crossref","first-page":"27735","DOI":"10.1038\/srep27735","article-title":"Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor","volume":"6","author":"Postel","year":"2016","journal-title":"Sci. Rep"},{"issue":"1","key":"10.1016\/j.virusres.2020.197975_bib0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-016-0406-1","article-title":"Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence","volume":"48","author":"Schwarz","year":"2017","journal-title":"Vet. Res."},{"issue":"2","key":"10.1016\/j.virusres.2020.197975_bib0100","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00459-19","article-title":"Dengue and Zika Virus 5\u2019 Untranslated Regions Harbor Internal Ribosomal Entry Site Functions","volume":"10","author":"Song","year":"2019","journal-title":"mBio"},{"issue":"10","key":"10.1016\/j.virusres.2020.197975_bib0105","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0140900","article-title":"Interactions between Hepatitis C Virus and the Human Apolipoprotein H Acute Phase Protein: A Tool for a Sensitive Detection of the Virus","volume":"10","author":"Stefas","year":"2015","journal-title":"PLoS One"},{"issue":"7","key":"10.1016\/j.virusres.2020.197975_bib0110","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01997-17","article-title":"Roles of the 5\u2019 Untranslated Region of Nonprimate Hepacivirus in Translation Initiation and Viral Replication","volume":"92","author":"Tanaka","year":"2018","journal-title":"J. Virol."},{"issue":"19","key":"10.1016\/j.virusres.2020.197975_bib0115","doi-asserted-by":"crossref","first-page":"796","DOI":"10.4254\/wjh.v8.i19.796","article-title":"Assembly and release of infectious hepatitis C virus involving unusual organization of the secretory pathway","volume":"8","author":"Triyatni","year":"2016","journal-title":"World. J. Hepatol."},{"issue":"3","key":"10.1016\/j.virusres.2020.197975_bib0120","doi-asserted-by":"crossref","first-page":"169","DOI":"10.5114\/ceh.2017.68287","article-title":"Megamitochondria formation in hepatocytes of patient with chronic hepatitis C - a case report","volume":"3","author":"Wieczorek","year":"2017","journal-title":"Clin. Exp. Hepatol."},{"key":"10.1016\/j.virusres.2020.197975_bib0125","doi-asserted-by":"crossref","first-page":"862","DOI":"10.3389\/fmicb.2017.00862","article-title":"Atypical Porcine Pestivirus as a Novel Type of Pestivirus in Pigs in China","volume":"8","author":"Yuan","year":"2017","journal-title":"Front. Microbiol."},{"issue":"4","key":"10.1016\/j.virusres.2020.197975_bib0130","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1111\/tbed.12659","article-title":"Identification of atypical porcine pestivirus infection in swine herds in China","volume":"64","author":"Zhang","year":"2017","journal-title":"Transbound. Emerg. Dis."}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170220301428?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170220301428?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:09:37Z","timestamp":1759154977000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170220301428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":26,"alternative-id":["S0168170220301428"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2020.197975","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of the reverse genetics system for emerging atypical porcine pestivirus using in vitro and intracellular transcription systems","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2020.197975","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"197975"}}