{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:46:02Z","timestamp":1784335562342,"version":"3.55.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.virusres.2022.198789","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T02:23:37Z","timestamp":1650939817000},"page":"198789","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Minor and major envelope proteins of PRRSV play synergistic roles in inducing heterologous neutralizing antibodies and conferring cross protection"],"prefix":"10.1016","volume":"315","author":[{"given":"Jixiang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinshuai","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binghui","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanglong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-8760","authenticated-orcid":false,"given":"Nanhua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"46","key":"10.1016\/j.virusres.2022.198789_bib0001","doi-asserted-by":"crossref","first-page":"5540","DOI":"10.1016\/j.vaccine.2016.09.048","article-title":"Commercial vaccines provide limited protection to NADC30-like PRRSV infection","volume":"34","author":"Bai","year":"2016","journal-title":"Vaccine"},{"issue":"Pt 4","key":"10.1016\/j.virusres.2022.198789_bib0002","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1099\/vir.0.027995-0","article-title":"Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China","volume":"92","author":"Chen","year":"2011","journal-title":"J. Gen. Virol."},{"issue":"5","key":"10.1016\/j.virusres.2022.198789_bib0003","first-page":"1820","article-title":"The infectious cDNA clone of commercial HP-PRRS JXA1-R-attenuated vaccine can be a potential effective live vaccine vector","volume":"67","author":"Chen","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0004","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s13567-021-00944-8","article-title":"Chimeric HP-PRRSV2 containing an ORF2-6 consensus sequence induces antibodies with broadly neutralizing activity and confers cross protection against virulent NADC30-like isolate","volume":"52","author":"Chen","year":"2021","journal-title":"Vet. Res."},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0005","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/tbed.13026","article-title":"A novel NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) plays a limited role in the pathogenicity of porcine circoviruses (PCV2 and PCV3) and PRRSV co-infection","volume":"66","author":"Chen","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.virusres.2022.198789_bib0006","first-page":"167","article-title":"ORF5 of porcine reproductive and respiratory syndrome virus (PRRSV) is a target of diversifying selection as infection progresses from acute infection to virus rebound","volume":"40","author":"Chen","year":"2016","journal-title":"Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis."},{"key":"10.1016\/j.virusres.2022.198789_bib0007","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.vetimm.2017.06.005","article-title":"Amplification and selection of PRRSV-activated VDJ repertoires in pigs secreting distinct neutralizing antiboidies","volume":"189","author":"Chen","year":"2017","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"9","key":"10.1016\/j.virusres.2022.198789_bib0008","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3390\/v11090875","article-title":"Identification of two porcine reproductive and respiratory syndrome virus variants sharing high genomic homology but with distinct virulence","volume":"11","author":"Chen","year":"2019","journal-title":"Viruses"},{"issue":"6","key":"10.1016\/j.virusres.2022.198789_bib0009","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1111\/tbed.12952","article-title":"Emergence of a novel highly pathogenic recombinant virus from three lineages of porcine reproductive and respiratory syndrome virus 2 in China 2017","volume":"65","author":"Chen","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"issue":"6","key":"10.1016\/j.virusres.2022.198789_bib0010","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1111\/tbed.13276","article-title":"Development of universal and quadruplex real-time RT-PCR assays for simultaneous detection and differentiation of porcine reproductive and respiratory syndrome viruses","volume":"66","author":"Chen","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"issue":"11","key":"10.1016\/j.virusres.2022.198789_bib0011","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1080\/14760584.2019.1690458","article-title":"Strategies for inducing effective neutralizing antibody responses against HIV-1","volume":"18","author":"Del Moral-Sanchez","year":"2019","journal-title":"Expert Rev. Vaccines"},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0012","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12985-017-0910-6","article-title":"The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective","volume":"15","author":"Guo","year":"2018","journal-title":"Virol. J."},{"issue":"4","key":"10.1016\/j.virusres.2022.198789_bib0013","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1093\/molbev\/msi089","article-title":"The origin and evolution of porcine reproductive and respiratory syndrome viruses","volume":"22","author":"Hanada","year":"2005","journal-title":"Mol. Biol. Evol."},{"issue":"24","key":"10.1016\/j.virusres.2022.198789_bib0014","doi-asserted-by":"crossref","first-page":"13456","DOI":"10.1128\/JVI.02435-13","article-title":"Highly divergent strains of porcine reproductive and respiratory syndrome virus incorporate multiple isoforms of nonstructural protein 2 into virions","volume":"87","author":"Kappes","year":"2013","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0015","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1186\/s12985-017-0825-2","article-title":"ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro","volume":"14","author":"Leng","year":"2017","journal-title":"Virol. J."},{"issue":"3","key":"10.1016\/j.virusres.2022.198789_bib0016","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.vetimm.2004.09.005","article-title":"Role of neutralizing antibodies in PRRSV protective immunity","volume":"102","author":"Lopez","year":"2004","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.virusres.2022.198789_bib0017","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev-animal-022114-111025","article-title":"Porcine reproductive and respiratory syndrome virus (PRRSV): pathogenesis and interaction with the immune system","volume":"4","author":"Lunney","year":"2016","journal-title":"Annu. Rev. Anim. Biosci."},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0018","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1006\/viro.2002.1612","article-title":"Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity","volume":"302","author":"Osorio","year":"2002","journal-title":"Virology"},{"issue":"9","key":"10.1016\/j.virusres.2022.198789_bib0019","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1128\/JVI.76.9.4241-4250.2002","article-title":"Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain","volume":"76","author":"Ostrowski","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2022.198789_bib0020","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.vetmic.2017.04.016","article-title":"GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) as a target for homologous and broadly neutralizing antibodies","volume":"209","author":"Popescu","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.virusres.2022.198789_bib0021","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.virusres.2015.03.016","article-title":"Broadly neutralizing antibodies against the rapidly evolving porcine reproductive and respiratory syndrome virus","volume":"203","author":"Robinson","year":"2015","journal-title":"Virus Res."},{"issue":"17","key":"10.1016\/j.virusres.2022.198789_bib0022","doi-asserted-by":"crossref","first-page":"8700","DOI":"10.1128\/JVI.02551-09","article-title":"Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses","volume":"84","author":"Shi","year":"2010","journal-title":"J. Virol."},{"issue":"6","key":"10.1016\/j.virusres.2022.198789_bib0023","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1007\/s12250-019-00149-6","article-title":"Nsp2 and GP5-M of porcine reproductive and respiratory syndrome virus contribute to targets for neutralizing antibodies","volume":"34","author":"Su","year":"2019","journal-title":"Virol. Sin."},{"key":"10.1016\/j.virusres.2022.198789_bib0024","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.vetmic.2018.02.016","article-title":"Efficacy evaluation of two commercial modified-live virus vaccines against a novel recombinant type 2 porcine reproductive and respiratory syndrome virus","volume":"216","author":"Sun","year":"2018","journal-title":"Vet. Microbiol."},{"issue":"18","key":"10.1016\/j.virusres.2022.198789_bib0025","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1016\/j.vaccine.2017.03.046","article-title":"Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains","volume":"35","author":"Tian","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.virusres.2022.198789_bib0026","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.virol.2015.08.021","article-title":"Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs","volume":"485","author":"Tian","year":"2015","journal-title":"Virology"},{"issue":"6","key":"10.1016\/j.virusres.2022.198789_bib0027","doi-asserted-by":"crossref","first-page":"e526","DOI":"10.1371\/journal.pone.0000526","article-title":"Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark","volume":"2","author":"Tian","year":"2007","journal-title":"PLoS One"},{"issue":"12","key":"10.1016\/j.virusres.2022.198789_bib0028","doi-asserted-by":"crossref","first-page":"6515","DOI":"10.1128\/JVI.03287-14","article-title":"A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity","volume":"89","author":"Trible","year":"2015","journal-title":"J. Virol."},{"issue":"29-30","key":"10.1016\/j.virusres.2022.198789_bib0029","doi-asserted-by":"crossref","first-page":"4794","DOI":"10.1016\/j.vaccine.2011.04.071","article-title":"Characterization of antigenic regions in the porcine reproductive and respiratory syndrome virus by the use of peptide-specific serum antibodies","volume":"29","author":"Vanhee","year":"2011","journal-title":"Vaccine"},{"issue":"23","key":"10.1016\/j.virusres.2022.198789_bib0030","doi-asserted-by":"crossref","first-page":"12070","DOI":"10.1128\/JVI.01657-15","article-title":"A synthetic porcine reproductive and respiratory syndrome virus strain confers unprecedented levels of heterologous protection","volume":"89","author":"Vu","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2022.198789_bib0031","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2020.104716","article-title":"Broad neutralization activity against both PRRSV-1 and PRRSV-2 and enhancement of cell mediated immunity against PRRSV by a novel IgM monoclonal antibody","volume":"175","author":"Wu","year":"2020","journal-title":"Antivir. Res."},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0032","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s13567-021-00914-0","article-title":"A broadly neutralizing monoclonal antibody induces broad protection against heterogeneous PRRSV strains in piglets","volume":"52","author":"Zhang","year":"2021","journal-title":"Vet. Res."},{"issue":"20","key":"10.1016\/j.virusres.2022.198789_bib0033","doi-asserted-by":"crossref","first-page":"10712","DOI":"10.1128\/JVI.01446-15","article-title":"Importation and recombination are responsible for the latest emergence of highly pathogenic porcine reproductive and respiratory syndrome virus in China","volume":"89","author":"Zhao","year":"2015","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2022.198789_bib0034","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.virol.2012.09.005","article-title":"DNA shuffling of the GP3 genes of porcine reproductive and respiratory syndrome virus (PRRSV) produces a chimeric virus with an improved cross-neutralizing ability against a heterologous PRRSV strain","volume":"434","author":"Zhou","year":"2012","journal-title":"Virology"},{"key":"10.1016\/j.virusres.2022.198789_bib0035","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.vetmic.2017.05.031","article-title":"Efficacy evaluation of three modified-live virus vaccines against a strain of porcine reproductive and respiratory syndrome virus NADC30-like","volume":"207","author":"Zhou","year":"2017","journal-title":"Vet. Microbiol."}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170222001162?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170222001162?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:17:47Z","timestamp":1759155467000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170222001162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":35,"alternative-id":["S0168170222001162"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2022.198789","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Minor and major envelope proteins of PRRSV play synergistic roles in inducing heterologous neutralizing antibodies and conferring cross protection","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2022.198789","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"198789"}}