{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:26:16Z","timestamp":1789709176789,"version":"4.0.1"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T00:00:00Z","timestamp":1695600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2023,11]]},"DOI":"10.1016\/j.virusres.2023.199230","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T12:54:40Z","timestamp":1695992080000},"page":"199230","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["In-cell Western assay to quantify infection with pathogenic orthohantavirus Puumala virus in replication kinetics and antiviral drug testing"],"prefix":"10.1016","volume":"337","author":[{"given":"Christian","family":"Nusshag","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Uhrig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Zeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Krautkr\u00e4mer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.virusres.2023.199230_bib0001","doi-asserted-by":"crossref","first-page":"494","DOI":"10.3109\/00365548.2012.755268","article-title":"A severe case of Puumala hantavirus infection successfully treated with bradykinin receptor antagonist icatibant","volume":"45","author":"Antonen","year":"2013","journal-title":"Scand. J. Infect. Dis."},{"issue":"9","key":"10.1016\/j.virusres.2023.199230_bib0002","doi-asserted-by":"crossref","DOI":"10.3390\/v11090855","article-title":"Puumala and tula virus differ in replication kinetics and innate immune stimulation in human endothelial cells and macrophages","volume":"11","author":"Bourquain","year":"2019","journal-title":"Viruses"},{"issue":"5","key":"10.1016\/j.virusres.2023.199230_bib0003","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3851\/IMP1729","article-title":"Maporal virus as a surrogate for pathogenic New World hantaviruses and its inhibition by favipiravir","volume":"21","author":"Buys","year":"2011","journal-title":"Antivir. Chem. Chemother."},{"issue":"21","key":"10.1016\/j.virusres.2023.199230_bib0004","doi-asserted-by":"crossref","first-page":"11722","DOI":"10.1128\/JVI.00874-07","article-title":"Ribavirin reveals a lethal threshold of allowable mutation frequency for Hantaan virus","volume":"81","author":"Chung","year":"2007","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0005","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1099\/0022-1317-43-1-247","article-title":"Regulation of the interferon system: evidence that Vero cells have a genetic defect in interferon production","volume":"43","author":"Emeny","year":"1979","journal-title":"J. Gen. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0006","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1186\/s12879-018-3583-x","article-title":"Motility of human renal cells is disturbed by infection with pathogenic hantaviruses","volume":"18","author":"H\u00e4gele","year":"2018","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.virusres.2023.199230_bib0007","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.virusres.2018.11.015","article-title":"Virus- and cell type-specific effects in orthohantavirus infection","volume":"260","author":"H\u00e4gele","year":"2019","journal-title":"Virus Res."},{"issue":"6","key":"10.1016\/j.virusres.2023.199230_bib0008","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1093\/infdis\/164.6.1119","article-title":"Prospective, double-blind, concurrent, placebo-controlled clinical trial of intravenous ribavirin therapy of hemorrhagic fever with renal syndrome","volume":"164","author":"Huggins","year":"1991","journal-title":"J. Infect. Dis."},{"issue":"9","key":"10.1016\/j.virusres.2023.199230_bib0009","doi-asserted-by":"crossref","first-page":"4538","DOI":"10.1128\/JVI.02280-08","article-title":"Reduced ribavirin antiviral efficacy via nucleoside transporter-mediated drug resistance","volume":"83","author":"Ibarra","year":"2009","journal-title":"J. Virol."},{"issue":"8","key":"10.1016\/j.virusres.2023.199230_bib0010","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1038\/sj.bjp.0701775","article-title":"Ribavirin uptake by human erythrocytes and the involvement of nitrobenzylthioinosine-sensitive (es)-nucleoside transporters","volume":"123","author":"Jarvis","year":"1998","journal-title":"Br. J. Pharmacol."},{"issue":"3","key":"10.1016\/j.virusres.2023.199230_bib0011","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s00705-012-1514-5","article-title":"Complex evolution and epidemiology of Dobrava-Belgrade hantavirus: definition of genotypes and their characteristics","volume":"158","author":"Klempa","year":"2013","journal-title":"Arch. Virol."},{"issue":"7","key":"10.1016\/j.virusres.2023.199230_bib0012","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/ckj\/sfac008","article-title":"The kidney in hantavirus infection-epidemiology, virology, pathophysiology, clinical presentation, diagnosis and management","volume":"15","author":"Koehler","year":"2022","journal-title":"Clin. Kidney J."},{"issue":"8","key":"10.1016\/j.virusres.2023.199230_bib0013","doi-asserted-by":"crossref","first-page":"E358","DOI":"10.1111\/1469-0691.12218","article-title":"Viral load and immune response dynamics in patients with haemorrhagic fever with renal syndrome","volume":"19","author":"Korva","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"issue":"19","key":"10.1016\/j.virusres.2023.199230_bib0014","doi-asserted-by":"crossref","first-page":"9811","DOI":"10.1128\/JVI.00568-11","article-title":"Pathogenic old world hantaviruses infect renal glomerular and tubular cells and induce disassembling of cell-to-cell contacts","volume":"85","author":"Krautkr\u00e4mer","year":"2011","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0015","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/ki.2012.360","article-title":"Hantavirus infection: an emerging infectious disease causing acute renal failure","volume":"83","author":"Krautkr\u00e4mer","year":"2013","journal-title":"Kidney Int."},{"issue":"2","key":"10.1016\/j.virusres.2023.199230_bib0016","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3109\/00365548.2014.969304","article-title":"Severe Puumala virus infection in a patient with a lymphoproliferative disease treated with icatibant","volume":"47","author":"Laine","year":"2015","journal-title":"Infect. Dis."},{"key":"10.1016\/j.virusres.2023.199230_bib0017","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3389\/fcimb.2017.00269","article-title":"In-cell western assays to evaluate hantaan virus replication as a novel approach to screen antiviral molecules and detect neutralizing antibody titers","volume":"7","author":"Ma","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"7","key":"10.1016\/j.virusres.2023.199230_bib0018","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1080\/23744235.2017.1293841","article-title":"Insufficient efficacy and safety of intravenous ribavirin in treatment of haemorrhagic fever with renal syndrome caused by Puumala virus","volume":"49","author":"Malinin","year":"2017","journal-title":"Infect. Dis."},{"issue":"6","key":"10.1016\/j.virusres.2023.199230_bib0019","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms9061306","article-title":"Antiviral efficacy of ribavirin and favipiravir against hantaan virus","volume":"9","author":"Mayor","year":"2021","journal-title":"Microorganisms"},{"issue":"8","key":"10.1016\/j.virusres.2023.199230_bib0020","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1007\/s007050070077","article-title":"I antiviral activity of lactoferrin and ribavirin upon hantavirus","volume":"145","author":"Murphy","year":"2000","journal-title":"Arch. Virol."},{"issue":"9","key":"10.1016\/j.virusres.2023.199230_bib0021","doi-asserted-by":"crossref","first-page":"2554","DOI":"10.1681\/ASN.2006070732","article-title":"Mizoribine corrects defective nephrin biogenesis by restoring intracellular energy balance","volume":"18","author":"Nakajo","year":"2007","journal-title":"J. Am. Soc. Nephrol."},{"issue":"4","key":"10.1016\/j.virusres.2023.199230_bib0022","doi-asserted-by":"crossref","DOI":"10.3390\/v14040823","article-title":"Expression profile of human renal mesangial cells is altered by infection with pathogenic puumala orthohantavirus","volume":"14","author":"Nusshag","year":"2022","journal-title":"Viruses"},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0023","doi-asserted-by":"crossref","first-page":"19117","DOI":"10.1038\/s41598-020-76050-0","article-title":"Glomerular filtration barrier dysfunction in a self-limiting, RNA virus-induced glomerulopathy resembles findings in idiopathic nephrotic syndromes","volume":"10","author":"Nusshag","year":"2020","journal-title":"Sci. Rep."},{"issue":"5","key":"10.1016\/j.virusres.2023.199230_bib0024","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0216700","article-title":"Three asymptomatic animal infection models of hemorrhagic fever with renal syndrome caused by hantaviruses","volume":"14","author":"Perley","year":"2019","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.virusres.2023.199230_bib0025","doi-asserted-by":"crossref","first-page":"749","DOI":"10.18388\/abp.2005_3386","article-title":"Recent advances in studies on biochemical and structural properties of equilibrative and concentrative nucleoside transporters","volume":"52","author":"Podgorska","year":"2005","journal-title":"Acta Biochim. Pol."},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0026","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.antiviral.2008.09.007","article-title":"Experience with intravenous ribavirin in the treatment of hemorrhagic fever with renal syndrome in Korea","volume":"81","author":"Rusnak","year":"2009","journal-title":"Antiviral Res."},{"issue":"3","key":"10.1016\/j.virusres.2023.199230_bib0027","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1681\/ASN.V133630","article-title":"A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression","volume":"13","author":"Saleem","year":"2002","journal-title":"J. Am. Soc. Nephrol."},{"issue":"1\u20132","key":"10.1016\/j.virusres.2023.199230_bib0028","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.virusres.2011.05.021","article-title":"Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts","volume":"160","author":"Sanada","year":"2011","journal-title":"Virus Res."},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0029","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1128\/JVI.77.1.481-488.2003","article-title":"Ribavirin causes error catastrophe during hantaan virus replication","volume":"77","author":"Severson","year":"2003","journal-title":"J. Virol."},{"issue":"6","key":"10.1016\/j.virusres.2023.199230_bib0030","doi-asserted-by":"crossref","first-page":"e11265","DOI":"10.1371\/journal.pone.0011265","article-title":"Cell type mediated resistance of vesicular stomatitis virus and Sendai virus to ribavirin","volume":"5","author":"Shah","year":"2010","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.virusres.2023.199230_bib0031","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1177\/095632020101200602","article-title":"Antiviral activity and mode of action studies of ribavirin and mycophenolic acid against orthopoxviruses in vitro","volume":"12","author":"Smee","year":"2001","journal-title":"Antivir. Chem. Chemother."},{"issue":"2","key":"10.1016\/j.virusres.2023.199230_bib0032","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms11020283","article-title":"Fatal puumala hantavirus infection in a patient with common variable immunodeficiency (CVID)","volume":"11","author":"Steininger","year":"2023","journal-title":"Microorganisms"},{"issue":"1","key":"10.1016\/j.virusres.2023.199230_bib0033","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1128\/AAC.00790-06","article-title":"Activity of ribavirin against Hantaan virus correlates with production of ribavirin-5\u2032-triphosphate, not with inhibition of IMP dehydrogenase","volume":"51","author":"Sun","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"issue":"Pt 3","key":"10.1016\/j.virusres.2023.199230_bib0034","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1099\/0022-1317-74-3-515","article-title":"Susceptibility of human cells to Puumala virus infection","volume":"74","author":"Temonen","year":"1993","journal-title":"J. Gen. Virol."},{"issue":"10","key":"10.1016\/j.virusres.2023.199230_bib0035","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1080\/23744235.2022.2076904","article-title":"Clinical and genomic characterisation of a fatal Puumala orthohantavirus case with low levels of neutralising antibodies","volume":"54","author":"Tuiskunen B\u00e4ck","year":"2022","journal-title":"Infect. Dis."},{"issue":"8","key":"10.1016\/j.virusres.2023.199230_bib0036","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/nrmicro3066","article-title":"Uncovering the mysteries of hantavirus infections","volume":"11","author":"Vaheri","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"issue":"2","key":"10.1016\/j.virusres.2023.199230_bib0037","doi-asserted-by":"crossref","first-page":"658","DOI":"10.3201\/eid2702.203770","article-title":"Puumala virus infection in family, Switzerland","volume":"27","author":"Vetter","year":"2021","journal-title":"Emerg. Infect. Dis."},{"issue":"7","key":"10.1016\/j.virusres.2023.199230_bib0038","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.3390\/v6072723","article-title":"Increased plasma cell-free DNA level during HTNV infection: correlation with disease severity and virus load","volume":"6","author":"Yi","year":"2014","journal-title":"Viruses"},{"issue":"2","key":"10.1016\/j.virusres.2023.199230_bib0039","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A simple statistical parameter for use in evaluation and validation of high throughput screening assays","volume":"4","author":"Zhang","year":"1999","journal-title":"J. Biomol. Screen"}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170223001922?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170223001922?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:20:41Z","timestamp":1759155641000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170223001922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":39,"alternative-id":["S0168170223001922"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2023.199230","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2023,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In-cell Western assay to quantify infection with pathogenic orthohantavirus Puumala virus in replication kinetics and antiviral drug testing","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2023.199230","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"199230"}}