{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:24:16Z","timestamp":1789471456773,"version":"build-2803163510"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T00:00:00Z","timestamp":1726876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902119"],"award-info":[{"award-number":["81902119"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Province Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2021MH214"],"award-info":[{"award-number":["ZR2021MH214"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.virusres.2024.199473","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T06:40:44Z","timestamp":1727764844000},"page":"199473","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Isolation and characterization of lytic bacteriophage vB_KpnP_23: A promising antimicrobial candidate against carbapenem-resistant Klebsiella pneumoniae"],"prefix":"10.1016","volume":"350","author":[{"given":"Qian","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinmei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinglun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5387-7971","authenticated-orcid":false,"given":"Yingying","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.virusres.2024.199473_bib0001","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3390\/antibiotics10020124","article-title":"Phage-encoded endolysins","volume":"10","author":"Abdelrahman","year":"2021","journal-title":"Antibiotics."},{"issue":"14","key":"10.1016\/j.virusres.2024.199473_bib0002","doi-asserted-by":"crossref","first-page":"4126","DOI":"10.1093\/bioinformatics\/btaa490","article-title":"Metaviral SPAdes: assembly of viruses from metagenomic data","volume":"36","author":"Antipov","year":"2020","journal-title":"Bioinformatics."},{"key":"10.1016\/j.virusres.2024.199473_bib0003","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3390\/ijms23094577","article-title":"Phage therapy in the era of multidrug resistance in bacteria: a systematic review","volume":"23","author":"Aranaga","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.virusres.2024.199473_bib0004","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.2147\/IDR.S173867","article-title":"Antibiotic resistance: a rundown of a global crisis","volume":"11","author":"Aslam","year":"2018","journal-title":"Infect. Drug Resist."},{"issue":"2","key":"10.1016\/j.virusres.2024.199473_bib0005","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1093\/femsre\/fuy043","article-title":"Klebsiella pneumoniae infection biology: living to counteract host defences","volume":"43","author":"Bengoechea","year":"2019","journal-title":"FEMS Microbiol. Rev."},{"issue":"4","key":"10.1016\/j.virusres.2024.199473_bib0006","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1093\/bioinformatics\/btz715","article-title":"Pavian: interactive analysis of metagenomics data for microbiome studies and pathogen identification","volume":"36","author":"Breitwieser","year":"2020","journal-title":"Bioinformatics."},{"issue":"7","key":"10.1016\/j.virusres.2024.199473_bib0007","doi-asserted-by":"crossref","first-page":"3774","DOI":"10.1128\/AAC.02668-14","article-title":"Art-175 is a highly efficient antibacterial against multidrug-resistant strains and Persisters of pseudomonas aeruginosa","volume":"58","author":"Briers","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"issue":"16","key":"10.1016\/j.virusres.2024.199473_bib0008","doi-asserted-by":"crossref","first-page":"9077","DOI":"10.1093\/nar\/gkab688","article-title":"tRNAscan-SE 2.0: improved detection and functional classification of transfer RNA genes","volume":"49","author":"Chan","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.virusres.2024.199473_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2022.114378","article-title":"Novel antimicrobial agents for combating antibiotic-resistant bacteria","volume":"187","author":"Chang","year":"2022","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"8","key":"10.1016\/j.virusres.2024.199473_bib0010","doi-asserted-by":"crossref","first-page":"e799","DOI":"10.1371\/journal.pone.0000799","article-title":"Phage-antibiotic synergy (PAS): \u03b2-lactam and quinolone antibiotics stimulate virulent phage growth","volume":"2","author":"Comeau","year":"2007","journal-title":"PLoS. One"},{"issue":"1","key":"10.1016\/j.virusres.2024.199473_bib0011","first-page":"19","article-title":"In Vitro Meropenem\/Antibiotic and Meropenem\/Bacteriophage Combinations against Carbapenem-Resistant gram-negative uropathogens","volume":"7","author":"Elshamy","year":"2023","journal-title":"Arch. Pharmaceut. Sci. Ain Shams Uni."},{"key":"10.1016\/j.virusres.2024.199473_bib0012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-019-1832-y","article-title":"OrthoFinder: phylogenetic orthology inference for comparative genomics","volume":"20","author":"Emms","year":"2019","journal-title":"Genome Biol."},{"issue":"5","key":"10.1016\/j.virusres.2024.199473_bib0013","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1084\/jem.20030857","article-title":"A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications","volume":"199","author":"Fang","year":"2004","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.virusres.2024.199473_bib0014","article-title":"Characterization and genome analysis of phage vB_KpnS_SXFY507 against Klebsiella pneumoniae and efficacy assessment in Galleria mellonella larvae","volume":"14","author":"Feng","year":"2023","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.virusres.2024.199473_bib0015","doi-asserted-by":"crossref","first-page":"4239","DOI":"10.1038\/s41467-022-31837-9","article-title":"Personalized bacteriophage therapy to treat Pandrug-resistant spinal Pseudomonas aeruginosa infection","volume":"13","author":"Ferry","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.virusres.2024.199473_bib0016","series-title":"Protein Identification and Analysis Tools On the ExPASy server","author":"Gasteiger","year":"2005"},{"issue":"suppl_2","key":"10.1016\/j.virusres.2024.199473_bib0017","doi-asserted-by":"crossref","first-page":"W181","DOI":"10.1093\/nar\/gkn179","article-title":"The CGView Server: a comparative genomics tool for circular genomes","volume":"36","author":"Grant","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.virusres.2024.199473_bib0018","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.771170","article-title":"Clonal dissemination of clinical carbapenem-resistant klebsiella pneumoniae isolates carrying fosA3 and bla KPC\u20132 coharboring plasmids in Shandong, China","volume":"12","author":"Hao","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.virusres.2024.199473_bib0019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ph12010035","article-title":"Phages for phage therapy: isolation, characterization, and host range breadth","volume":"12","author":"Hyman","year":"2019","journal-title":"Pharmaceuticals. (Basel)"},{"issue":"1","key":"10.1016\/j.virusres.2024.199473_bib0020","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries","volume":"9","author":"Jain","year":"2018","journal-title":"Nat. Commun."},{"issue":"8","key":"10.1016\/j.virusres.2024.199473_bib0021","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1080\/14787210.2019.1646126","article-title":"Factors determining phage stability\/activity: challenges in practical phage application","volume":"17","author":"Jo\u0144czyk-Matysiak","year":"2019","journal-title":"Expert. Rev. Anti. Infect. Ther."},{"issue":"1","key":"10.1016\/j.virusres.2024.199473_bib0023","doi-asserted-by":"crossref","first-page":"85","DOI":"10.2174\/1381612820666140905112311","article-title":"Bacteriophages and their enzymes in biofilm control","volume":"21","author":"K Chan","year":"2015","journal-title":"Curr. Pharm. Des."},{"issue":"2","key":"10.1016\/j.virusres.2024.199473_bib0022","article-title":"Carbapenem-resistant klebsiella pneumoniae: virulence factors, molecular epidemiology and latest updates in treatment options","volume":"12","author":"Karampatakis","year":"2023","journal-title":"Antibiotics. (Basel)"},{"issue":"6","key":"10.1016\/j.virusres.2024.199473_bib0024","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"issue":"12","key":"10.1016\/j.virusres.2024.199473_bib0025","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1101\/gr.210641.116","article-title":"Centrifuge: rapid and sensitive classification of metagenomic sequences","volume":"26","author":"Kim","year":"2016","journal-title":"Genome Res."},{"key":"10.1016\/j.virusres.2024.199473_bib0026","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.3389\/fmicb.2019.02949","article-title":"Diversity and function of phage encoded depolymerases","volume":"10","author":"Knecht","year":"2020","journal-title":"Front. Microbiol."},{"issue":"21","key":"10.1016\/j.virusres.2024.199473_bib0027","doi-asserted-by":"crossref","first-page":"4453","DOI":"10.1093\/bioinformatics\/btz305","article-title":"RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference","volume":"35","author":"Kozlov","year":"2019","journal-title":"Bioinformatics."},{"issue":"5","key":"10.1016\/j.virusres.2024.199473_bib0029","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1007\/s11262-021-01847-8","article-title":"Identification of a phage-derived depolymerase specific for KL64 capsule of Klebsiella pneumoniae and its anti-biofilm effect","volume":"57","author":"Li","year":"2021","journal-title":"Virus. Genes."},{"issue":"5","key":"10.1016\/j.virusres.2024.199473_bib0028","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1007\/s11262-022-01917-5","article-title":"Isolation and characterization of the novel bacteriophage vB_SmaS_BUCT626 against Stenotrophomonas maltophilia","volume":"58","author":"Li","year":"2022","journal-title":"Virus. Genes."},{"issue":"12","key":"10.1016\/j.virusres.2024.199473_bib0030","doi-asserted-by":"crossref","first-page":"4348","DOI":"10.1016\/j.apsb.2022.05.007","article-title":"Recent advances in bacteriophage-based therapeutics: insight into the post-antibiotic era","volume":"12","author":"Ling","year":"2022","journal-title":"Acta Pharm. Sin. B"},{"issue":"7","key":"10.1016\/j.virusres.2024.199473_bib0032","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1093\/bioinformatics\/17.7.646","article-title":"Evaluation of methods for the prediction of membrane spanning regions","volume":"17","author":"M\u00f6ller","year":"2001","journal-title":"Bioinformatics."},{"issue":"7459","key":"10.1016\/j.virusres.2024.199473_bib0031","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/499394a","article-title":"Antibiotic resistance: the last resort","volume":"499","author":"McKenna","year":"2013","journal-title":"Nature"},{"issue":"6","key":"10.1016\/j.virusres.2024.199473_bib0033","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/j.cmi.2023.03.011","article-title":"Bacteriophage therapy in humans","volume":"29","author":"Nir-Paz","year":"2023","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.virusres.2024.199473_bib0034","doi-asserted-by":"crossref","first-page":"559","DOI":"10.3389\/fmicb.2017.00559","article-title":"Intriguing interaction of bacteriophage-host association: an understanding in the era of omics","volume":"8","author":"Parmar","year":"2017","journal-title":"Front Microbiol."},{"issue":"15","key":"10.1016\/j.virusres.2024.199473_bib0035","doi-asserted-by":"crossref","first-page":"7449","DOI":"10.1128\/JVI.00385-15","article-title":"Phage therapy: a step forward in the treatment of pseudomonas aeruginosa Infections","volume":"89","author":"Pires","year":"2015","journal-title":"J. Virol."},{"issue":"10","key":"10.1016\/j.virusres.2024.199473_bib0036","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2217\/fmb.12.97","article-title":"Bacteriophage endolysins as novel antimicrobials","volume":"7","author":"Schmelcher","year":"2012","journal-title":"Future Microbiol."},{"issue":"2","key":"10.1016\/j.virusres.2024.199473_bib0037","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4161\/viru.22718","article-title":"Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed","volume":"4","author":"Shon","year":"2013","journal-title":"Virulence"},{"issue":"3","key":"10.1016\/j.virusres.2024.199473_bib0038","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.virusres.2024.199473_bib44","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3389\/fcimb.2019.00022","article-title":"Fighting pathogenic bacteria on two fronts: phages and antibiotics as combined strategy","volume":"9","author":"Tagliaferri","year":"2019","journal-title":"Front. cell. infect. microbiol."},{"issue":"7","key":"10.1016\/j.virusres.2024.199473_bib0039","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"issue":"7","key":"10.1016\/j.virusres.2024.199473_bib0040","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","article-title":"SignalP 6.0 predicts all five types of signal peptides using protein language models","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"issue":"4","key":"10.1016\/j.virusres.2024.199473_bib45","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/eva.12364","article-title":"Long\u2010term effects of single and combined introductions of antibiotics and bacteriophages on populations of Pseudomonas aeruginosa","volume":"9","author":"Torres\u2010Barcel\u00f3","year":"2016","journal-title":"Evol. Appl."},{"issue":"1","key":"10.1016\/j.virusres.2024.199473_bib0041","doi-asserted-by":"crossref","first-page":"5725","DOI":"10.1038\/s41467-022-33294-w","article-title":"Bacteriophage-antibiotic combination therapy against extensively drug-resistant Pseudomonas aeruginosa infection to allow liver transplantation in a toddler","volume":"13","author":"Van Nieuwenhuyse","year":"2022","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.virusres.2024.199473_bib0042","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1080\/22221751.2022.2049458","article-title":"Molecular epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae in China","volume":"11","author":"Yang","year":"2022","journal-title":"Emerg. Microbes. Infect."},{"issue":"4","key":"10.1016\/j.virusres.2024.199473_bib0043","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1128\/AAC.45.4.1151-1161.2001","article-title":"Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae","volume":"45","author":"Yigit","year":"2001","journal-title":"Antimicrob. Agents Chemother"}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170224001667?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170224001667?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T01:54:44Z","timestamp":1734486884000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170224001667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":45,"alternative-id":["S0168170224001667"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2024.199473","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation and characterization of lytic bacteriophage vB_KpnP_23: A promising antimicrobial candidate against carbapenem-resistant Klebsiella pneumoniae","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2024.199473","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"199473"}}