{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T03:56:44Z","timestamp":1793505404667,"version":"4.5.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.virusres.2024.199497","type":"journal-article","created":{"date-parts":[[2024,11,23]],"date-time":"2024-11-23T04:54:42Z","timestamp":1732337682000},"page":"199497","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Dominance of dengue virus serotype-2 in Pakistan (2023\u20132024): Molecular characterization of the envelope gene and exploration of antiviral targets"],"prefix":"10.1016","volume":"350","author":[{"given":"Haidar","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iffat","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Saad","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deeba","family":"Amraiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Al-Shahari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liaqat","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.virusres.2024.199497_bib0001","doi-asserted-by":"crossref","DOI":"10.4103\/0972-9062.331412","article-title":"An overview of dengue viral infection circulating in Pakistan","author":"Ali","year":"2022","journal-title":"J. Vector. Borne Dis."},{"key":"10.1016\/j.virusres.2024.199497_bib0002","article-title":"Complete genome sequence of dengue virus serotype 3 from Guangzhou, China","volume":"1","author":"Bai","year":"2013","journal-title":"Genome Announc."},{"key":"10.1016\/j.virusres.2024.199497_bib0003","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.574067","article-title":"Dengue virus non-structural protein 5 as a versatile, multi-functional effector in host\u2013pathogen interactions","author":"Bhatnagar","year":"2021","journal-title":"Front. Cell Infect. Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0004","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.virusres.2024.199497_bib0005","doi-asserted-by":"crossref","DOI":"10.1038\/sj.emboj.7600064","article-title":"Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation","volume":"23","author":"Bressanelli","year":"2004","journal-title":"EMBO J."},{"key":"10.1016\/j.virusres.2024.199497_bib0006","doi-asserted-by":"crossref","DOI":"10.1146\/annurev.mi.44.100190.003245","article-title":"Flavivirus genome organization, expression, and replication","author":"Chambers","year":"1990","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0007","doi-asserted-by":"crossref","DOI":"10.1016\/0035-9203(95)90412-3","article-title":"Dengue haemorrhagic fever outbreak in Karachi, Pakistan, 1994","volume":"89","author":"Chan","year":"1995","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.virusres.2024.199497_bib0008","doi-asserted-by":"crossref","DOI":"10.1046\/j.1365-3156.2002.00864.x","article-title":"Incidence and risk factors of probable dengue virus infection among Dutch travellers to Asia","volume":"7","author":"Cobelens","year":"2002","journal-title":"Trop. Med. Int. Health"},{"key":"10.1016\/j.virusres.2024.199497_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2013.05.130","article-title":"Complete genome sequencing and evolutionary analysis of Indian isolates of Dengue virus type 2","volume":"436","author":"Dash","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.virusres.2024.199497_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.chembiol.2018.05.011","article-title":"Inhibition of flaviviruses by targeting a conserved pocket on the viral envelope protein","volume":"25","author":"de Wispelaere","year":"2018","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.virusres.2024.199497_bib0010","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1716689115","article-title":"Pervasive tertiary structure in the dengue virus RNA genome","volume":"115","author":"Dethoff","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.virusres.2024.199497_bib0011","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2180-11-200","article-title":"Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan","volume":"11","author":"Fatima","year":"2011","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0012","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2017.00317","article-title":"Global epidemiology of dengue outbreaks in 1990\u20132015: a systematic review and meta-analysis","volume":"7","author":"Guo","year":"2017","journal-title":"Front. Cell Infect. Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0013","doi-asserted-by":"crossref","DOI":"10.3390\/v12080829","article-title":"Dengue: a mini review","author":"Harapan","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.virusres.2024.199497_bib0014","doi-asserted-by":"crossref","DOI":"10.3201\/eid1301.060376","article-title":"Dengue virus serotype 3, Karachi, Pakistan [9]","author":"Jamil","year":"2007","journal-title":"Emerg. Infect Dis."},{"key":"10.1016\/j.virusres.2024.199497_bib0015","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2018.00219","article-title":"Molecular characterization of dengue virus serotype 2 cosmospolitan genotype from 2015 dengue outbreak in Yunnan, China","volume":"8","author":"Jiang","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0016","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.jcv.2008.06.003","article-title":"Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan","volume":"43","author":"Khan","year":"2008","journal-title":"J. Clinic. Virol."},{"key":"10.1016\/j.virusres.2024.199497_bib0017","article-title":"Dengue in Pakistan : journey from a disease free to a Hyper Endemic Nation","volume":"5","author":"Khanani","year":"2011","journal-title":"J. Dow Univ. Health Sci. Karachi"},{"key":"10.1016\/j.virusres.2024.199497_bib0018","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02957-12","article-title":"Structure of a dengue virus envelope protein late-stage fusion intermediate","volume":"87","author":"Klein","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2024.199497_bib37","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction","volume":"30","author":"Lanciotti","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0019","doi-asserted-by":"crossref","DOI":"10.1136\/heartjnl-2020-317062","article-title":"Impact of cardiovascular disease and cardiac injury on in-hospital mortality in patients with COVID-19: a systematic review and meta-analysis","volume":"106","author":"Li","year":"2020","journal-title":"Heart"},{"key":"10.1016\/j.virusres.2024.199497_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2008.12.070","article-title":"Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production","volume":"379","author":"Limjindaporn","year":"2009","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.virusres.2024.199497_bib0021","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.79.2.1223-1231.2005","article-title":"Variable surface epitopes in the crystal structure of dengue virus type 3 envelope glycoprotein","volume":"79","author":"Modis","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2024.199497_bib0022","doi-asserted-by":"crossref","DOI":"10.1038\/nrmicro1067","article-title":"A structural perspective of the Flavivirus life cycle","author":"Mukhopadhyay","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.virusres.2024.199497_bib0023","doi-asserted-by":"crossref","unstructured":"Murugesan, A., Manoharan, M., 2020. Chapter 16 - Dengue Virus, in: Emerging and Reemerging Viral Pathogens.","DOI":"10.1016\/B978-0-12-819400-3.00016-8"},{"key":"10.1016\/j.virusres.2024.199497_bib0024","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph19127170","article-title":"Dengue seroprevalence and factors associated with dengue seropositivity in Petaling District, Malaysia","volume":"19","author":"Ng","year":"2022","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.virusres.2024.199497_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2018.10.016","article-title":"Emergence of dengue virus type 1 and type 3 as dominant serotypes during 2017 in Pune and Nashik regions of Maharashtra, Western India","volume":"66","author":"Patil","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.virusres.2024.199497_bib0026","article-title":"An epidemic of dengue fever in Karachi - Associated clinical manifestations","volume":"47","author":"Qureshi","year":"1997","journal-title":"J. Pak. Med. Assoc."},{"key":"10.1016\/j.virusres.2024.199497_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/0042-6822(89)90509-6","article-title":"Synthetic peptides derived from the deduced amino acid sequence of the E-glycoprotein of Murray Valley encephalitis virus elicit antiviral antibody","volume":"171","author":"Roehrig","year":"1989","journal-title":"Virology"},{"key":"10.1016\/j.virusres.2024.199497_bib0028","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005277","article-title":"Dengue virus non-structural protein 1 modulates infectious particle production via interaction with the structural proteins","volume":"11","author":"Scaturro","year":"2015","journal-title":"PLoS. Pathog."},{"key":"10.1016\/j.virusres.2024.199497_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002055","article-title":"Economic and Disease Burden of Dengue in Southeast Asia","volume":"7","author":"Shepard","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.virusres.2024.199497_bib0029","doi-asserted-by":"crossref","DOI":"10.3390\/molecules27030653","article-title":"Phytochemical compound screening to identify novel small molecules against dengue virus: a docking and dynamics study","volume":"27","author":"Shimu","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.virusres.2024.199497_bib0030","doi-asserted-by":"crossref","DOI":"10.3390\/vaccines11010163","article-title":"Genomic characterization of dengue virus outbreak in 2022 from Pakistan","volume":"11","author":"Umair","year":"2023","journal-title":"Vaccines"},{"key":"10.1016\/j.virusres.2024.199497_bib0031","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijid.2018.04.3853","article-title":"Evaluation of bio-efficacy and residual activity of pyriproxyfen against field collected aedes aegypti and aedes albopictus from Gujranwala (Punjab), Pakistan","volume":"73","author":"Usman","year":"2018","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.virusres.2024.199497_bib0032","article-title":"Scenario of dengue infection & its control in Pakistan: an up-date and way forward","author":"Yousaf","year":"2018","journal-title":"Asian Pac. J. Trop. Med."},{"key":"10.1016\/j.virusres.2024.199497_bib0033","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M112.384446","article-title":"The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation","volume":"287","author":"Zhang","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.virusres.2024.199497_bib0034","article-title":"Visualization of membrane protein domains by cryo-electron microscopy of dengue virus","volume":"10","author":"Zhang","year":"2003","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.virusres.2024.199497_bib0035","article-title":"Dengue structure differs at the temperatures of its human and mosquito hosts","volume":"110","author":"Zhang","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.virusres.2024.199497_bib0036","doi-asserted-by":"crossref","DOI":"10.1016\/j.str.2004.06.019","article-title":"Conformational changes of the flavivirus E glycoprotein","volume":"12","author":"Zhang","year":"2004","journal-title":"Structure"}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170224001904?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170224001904?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T06:55:46Z","timestamp":1734504946000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170224001904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":39,"alternative-id":["S0168170224001904"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2024.199497","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dominance of dengue virus serotype-2 in Pakistan (2023\u20132024): Molecular characterization of the envelope gene and exploration of antiviral targets","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2024.199497","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"199497"}}