{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T21:20:51Z","timestamp":1791753651847,"version":"4.3.4"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014825","name":"Biotechnology Industry Research Assistance Council","doi-asserted-by":"publisher","award":["BT\/NBM0101\/02\/18"],"award-info":[{"award-number":["BT\/NBM0101\/02\/18"]}],"id":[{"id":"10.13039\/501100014825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007865","name":"University of Texas Medical Branch","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR\/45261\/COT\/142\/30\/2022"],"award-info":[{"award-number":["BT\/PR\/45261\/COT\/142\/30\/2022"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.virusres.2025.199571","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T11:14:12Z","timestamp":1744197252000},"page":"199571","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Generation and characterization of infectious clones of chikungunya virus from an Indian strain as a resource towards chikungunya vaccine research"],"prefix":"10.1016","volume":"356","author":[{"given":"Garvita","family":"Mathur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakuntala","family":"Mahilkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujatha","family":"Sunil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.virusres.2025.199571_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.lansea.2023.100269","article-title":"Cytokine profile, neutralisation potential and viral replication dynamics in sera of chikungunya patients in India: a cross-sectional study","volume":"19","author":"Babu","year":"2023","journal-title":"The Lancet regional health. Southeast Asia"},{"issue":"2","key":"10.1016\/j.virusres.2025.199571_bib0007","doi-asserted-by":"crossref","first-page":"veab074","DOI":"10.1093\/ve\/veab074","article-title":"Chikungunya virus molecular evolution in India since its re-emergence in 2005","volume":"7","author":"Chaudhary","year":"2021","journal-title":"Virus Evol"},{"issue":"6","key":"10.1016\/j.virusres.2025.199571_bib0003","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02738-21","article-title":"Lineage divergence and vector-specific adaptation have driven Chikungunya virus onto multiple adaptive landscapes","volume":"12","author":"Chen","year":"2021","journal-title":"mBio"},{"issue":"8","key":"10.1016\/j.virusres.2025.199571_bib0004","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004960","article-title":"Antigenic variation of East\/Central\/South African and Asian Chikungunya virus genotypes in neutralization by Immune sera","volume":"10","author":"Chua","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"10.1016\/j.virusres.2025.199571_bib0021","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.chom.2024.02.011","article-title":"Pathophysiology of chikungunya virus infection associated with fatal outcomes","volume":"32","author":"de Souza","year":"2024","journal-title":"Cell Host Microbe"},{"issue":"4","key":"10.1016\/j.virusres.2025.199571_bib0001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1007706","article-title":"RNA recombination at Chikungunya virus 3\u2019UTR as an evolutionary mechanism that provides adaptability","volume":"15","author":"Filomatori","year":"2019","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.virusres.2025.199571_bib0016","doi-asserted-by":"crossref","first-page":"6084","DOI":"10.1128\/JVI.06449-11","article-title":"Attenuation of Chikungunya virus vaccine strain 181\/clone 25 is determined by two amino acid substitutions in the E2 envelope glycoprotein","volume":"86","author":"Gorchakov","year":"2012","journal-title":"J. Virol."},{"issue":"4","key":"10.1016\/j.virusres.2025.199571_bib0006","doi-asserted-by":"crossref","first-page":"857","DOI":"10.4269\/ajtmh.19-0481","article-title":"Chikungunya Outbreaks in India: a prospective study comparing neutralization and sequelae during two outbreaks in 2010 and 2016","volume":"102","author":"Jain","year":"2020","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.virusres.2025.199571_bib0010","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/cid\/cix283","article-title":"Clinical, serological, and virological analysis of 572 Chikungunya patients from 2010 to 2013 in India","volume":"65","author":"Jain","year":"2017","journal-title":"Clinical infectious diseases: an official publication of the Infectious Diseases Society of America"},{"issue":"Pt 9","key":"10.1016\/j.virusres.2025.199571_bib0013","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1099\/vir.0.043752-0","article-title":"Construction of an infectious Chikungunya virus cDNA clone and stable insertion of mCherry reporter genes at two different sites","volume":"93","author":"K\u00fcmmerer","year":"2012","journal-title":"J. Gen. Virol."},{"issue":"3","key":"10.1016\/j.virusres.2025.199571_bib0022","doi-asserted-by":"crossref","first-page":"568","DOI":"10.3390\/vaccines11030568","article-title":"Chikungunya Virus: priority pathogen or passing trend?","volume":"11","author":"Montalvo Zurbia-Flores","year":"2023","journal-title":"Vaccines (Basel)"},{"issue":"1","key":"10.1016\/j.virusres.2025.199571_bib0018","doi-asserted-by":"crossref","DOI":"10.1080\/21505594.2024.2401985","article-title":"Host-adaptive mutations in Chikungunya virus genome","volume":"15","author":"Ning","year":"2024","journal-title":"Virulence"},{"issue":"7","key":"10.1016\/j.virusres.2025.199571_bib0024","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002142","article-title":"Novel chikungunya vaccine candidate with an IRES-based attenuation and host range alteration mechanism","volume":"7","author":"Plante","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.virusres.2025.199571_bib0009","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/1743-422X-9-100","article-title":"Genetic characterization of Chikungunya virus from New Delhi reveal emergence of a new molecular signature in Indian isolates","volume":"9","author":"Shrinet","year":"2012","journal-title":"Virol J"},{"key":"10.1016\/j.virusres.2025.199571_bib0002","article-title":"Current status of Chikungunya in India","volume":"12","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.virusres.2025.199571_bib0017","doi-asserted-by":"crossref","first-page":"259","DOI":"10.4049\/jimmunol.1202177","article-title":"A pathogenic role for CD4+ T cells during Chikungunya virus infection in mice","volume":"190","author":"Teo","year":"2013","journal-title":"J Immunol"},{"key":"10.1016\/j.virusres.2025.199571_bib0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002412","article-title":"Sequential adaptive mutations enhance efficient vector switching by Chikungunya virus and its epidemic emergence","volume":"7","author":"Tsetsarkin","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.virusres.2025.199571_bib0008","doi-asserted-by":"crossref","first-page":"4084","DOI":"10.1038\/ncomms5084","article-title":"Multi-peaked adaptive landscape for chikungunya virus evolution predicts continued fitness optimization in Aedes albopictus mosquitoes","volume":"5","author":"Tsetsarkin","year":"2014","journal-title":"Nat Commun"},{"key":"10.1016\/j.virusres.2025.199571_bib0014","doi-asserted-by":"crossref","first-page":"e6835","DOI":"10.1371\/journal.pone.0006835","article-title":"Epistatic roles of E2 glycoprotein mutations in adaption of chikungunya virus to Aedes albopictus and Ae. aegypti mosquitoes","volume":"4","author":"Tsetsarkin","year":"2009","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.virusres.2025.199571_bib0015","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1089\/vbz.2006.6.325","article-title":"Infectious clones of Chikungunya virus (La R\u00e9union isolate) for vector competence studies","volume":"6","author":"Tsetsarkin","year":"2006","journal-title":"Vector Borne Zoonotic Dis."},{"issue":"10","key":"10.1016\/j.virusres.2025.199571_bib0020","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1016\/j.ibmb.2005.05.008","article-title":"Development and characterization of a double subgenomic chikungunya virus infectious clone to express heterologous genes in Aedes aegypti mosquitoes","volume":"35","author":"Vanlandingham","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.virusres.2025.199571_bib0011","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1007\/s00705-021-05049-3","article-title":"Chikungunya-specific IgG and neutralizing antibody responses in natural infection of Chikungunya virus in children from India","volume":"166","author":"Verma","year":"2021","journal-title":"Arch Virol"},{"key":"10.1016\/j.virusres.2025.199571_bib0023","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/s41541-020-00241-z","article-title":"Engineering a fidelity-variant live-attenuated vaccine for chikungunya virus","volume":"5","author":"Weiss","year":"2020","journal-title":"npj Vaccines"},{"issue":"5","key":"10.1016\/j.virusres.2025.199571_bib0012","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.chom.2020.04.004","article-title":"An infectious cDNA clone of SARS-CoV-2","volume":"27","author":"Xie","year":"2020","journal-title":"Cell Host Microbe"}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170225000486?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170225000486?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T12:10:52Z","timestamp":1762258252000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170225000486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":24,"alternative-id":["S0168170225000486"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2025.199571","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Generation and characterization of infectious clones of chikungunya virus from an Indian strain as a resource towards chikungunya vaccine research","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2025.199571","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 International Center for Genetic Engineering and Biotechnology. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"199571"}}