{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:42:10Z","timestamp":1773931330919,"version":"3.50.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:00:00Z","timestamp":1765670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Institutes of Health National Cancer Institute","doi-asserted-by":"publisher","award":["R01 CA258239"],"award-info":[{"award-number":["R01 CA258239"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virus Research"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.virusres.2025.199676","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T07:11:40Z","timestamp":1765782700000},"page":"199676","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Replication competent La Crosse virus pseudotyped VSV vector system as vaccine and serological diagnostic reagent"],"prefix":"10.1016","volume":"363","author":[{"given":"Ianko","family":"Iankov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun","family":"Ammayappan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Viker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Weisend","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Beard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandy","family":"Russell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elitza","family":"Theel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Ebihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8014-786X","authenticated-orcid":false,"given":"Evanthia","family":"Galanis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.virusres.2025.199676_bib0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinf.2024.106237","article-title":"Replication, safety and immunogenicity of the vectored Ebola vaccine rVSV-deltaG-ZEBOV-GP in a sub-Saharan African paediatric population: a randomised controlled, open-label trial in children aged 1-12 years living in Lambarene, Gabon","volume":"89","author":"Alabi","year":"2024","journal-title":"J. Infect."},{"key":"10.1016\/j.virusres.2025.199676_bib0025","doi-asserted-by":"crossref","first-page":"13543","DOI":"10.1128\/JVI.02240-13","article-title":"Characteristics of oncolytic vesicular stomatitis virus displaying tumor-targeting ligands","volume":"87","author":"Ammayappan","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0019","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1743-422X-5-25","article-title":"La Crosse virus infectivity, pathogenesis, and immunogenicity in mice and monkeys","volume":"5","author":"Bennett","year":"2008","journal-title":"Virol. J."},{"key":"10.1016\/j.virusres.2025.199676_bib0038","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1128\/JVI.02327-10","article-title":"A recombinant chimeric La Crosse virus expressing the surface glycoproteins of Jamestown Canyon virus is immunogenic and protective against challenge with either parental virus in mice or monkeys","volume":"86","author":"Bennett","year":"2012","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0008","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.3201\/eid2211.160308","article-title":"Epidemiology of La crosse virus emergence, Appalachia Region, United States","volume":"22","author":"Bewick","year":"2016","journal-title":"Emerg. Infect. Dis."},{"issue":"Pt 12","key":"10.1016\/j.virusres.2025.199676_bib0007","doi-asserted-by":"crossref","first-page":"3173","DOI":"10.1099\/0022-1317-80-12-3173","article-title":"Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoes","volume":"80","author":"Borucki","year":"1999","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0042","doi-asserted-by":"crossref","DOI":"10.1099\/jgv.0.001588","article-title":"Induction of protective immune responses against a lethal Zika virus challenge post-vaccination with a dual serotype of recombinant vesicular stomatitis virus carrying the genetically modified Zika virus E protein gene","volume":"102","author":"Choi","year":"2021","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0013","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1016\/j.cell.2022.01.019","article-title":"Structural and functional characterizations of infectivity and immune evasion of SARS-CoV-2 Omicron","volume":"185","author":"Cui","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.virusres.2025.199676_bib0036","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1093\/cid\/ciz1165","article-title":"Postexposure prophylaxis With rVSV-ZEBOV following exposure to a patient with Ebola virus disease relapse in the United Kingdom: an operational, safety, and immunogenicity report","volume":"71","author":"Davis","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.virusres.2025.199676_bib0011","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1186\/s13071-020-04440-4","article-title":"Local persistence of novel regional variants of La Crosse virus in the Northeast USA","volume":"13","author":"Eastwood","year":"2020","journal-title":"Parasit. Vectors."},{"key":"10.1016\/j.virusres.2025.199676_bib0030","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/j.vaccine.2011.12.085","article-title":"Evaluation of attenuated VSVs with mutated M or\/and G proteins as vaccine vectors","volume":"30","author":"Fang","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.virusres.2025.199676_bib0001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0882-4010(88)90041-1","article-title":"Genetic determinants of the virulence and infectivity of La Crosse virus","volume":"4","author":"Gonzalez-Scarano","year":"1988","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.virusres.2025.199676_bib0032","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1128\/CVI.00008-08","article-title":"Development of a novel efficient fluorescence-based plaque reduction microneutralization assay for measles virus immunity","volume":"15","author":"Haralambieva","year":"2008","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.virusres.2025.199676_bib0002","doi-asserted-by":"crossref","first-page":"e66","DOI":"10.1017\/S0950268818003096","article-title":"La Crosse virus: a scoping review of the global evidence","volume":"147","author":"Harding","year":"2018","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.virusres.2025.199676_bib0005","unstructured":"Hunt W.G., McJunkin J.E. in Feigin and Cherry's textbook of pediatric infectious diseases Ch. 190, 1866\u20131876.e1863 (2019)."},{"key":"10.1016\/j.virusres.2025.199676_bib0026","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.omto.2020.09.006","article-title":"Live attenuated measles virus vaccine expressing helicobacter pylori heat shock protein A","volume":"19","author":"Iankov","year":"2020","journal-title":"Mol. Ther. Oncolytics."},{"key":"10.1016\/j.virusres.2025.199676_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.jim.2021.112996","article-title":"Parameters of immunoglobulin extraction from dried blood spot cards and immunoassays for detection of antibody response to pathogens including the novel SARS-CoV-2","volume":"492","author":"Iankov","year":"2021","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.virusres.2025.199676_bib0009","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1603\/ME12023","article-title":"La Crosse virus infection alters blood feeding behavior in Aedes triseriatus and Aedes albopictus (Diptera: culicidae)","volume":"49","author":"Jackson","year":"2012","journal-title":"J. Med. Entomol."},{"issue":"Pt 3","key":"10.1016\/j.virusres.2025.199676_bib0023","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1099\/0022-1317-71-3-523","article-title":"Monoclonal antibodies specific for the G1 glycoprotein of La Crosse virus that react with other California serogroup viruses","volume":"71","author":"Kingsford","year":"1990","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0043","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0042-6822(84)90163-6","article-title":"Enhanced neutralization of La Crosse virus by the binding of specific pairs of monoclonal antibodies to the G1 glycoprotein","volume":"136","author":"Kingsford","year":"1984","journal-title":"Virology"},{"key":"10.1016\/j.virusres.2025.199676_bib0014","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1093\/oxfordjournals.aje.a113158","article-title":"Genotypic varieties of La Crosse virus isolated from different geographic regions of the continental United States and evidence for a naturally occurring intertypic recombinant La Crosse virus","volume":"114","author":"Klimas","year":"1981","journal-title":"Am. J. Epidemiol."},{"key":"10.1016\/j.virusres.2025.199676_bib0033","doi-asserted-by":"crossref","first-page":"256","DOI":"10.4269\/ajtmh.17-0889","article-title":"Dried Blood Spots for Global Health Diagnostics and Surveillance: opportunities and Challenges","volume":"99","author":"Lim","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.virusres.2025.199676_bib0017","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/0042-6822(91)90889-J","article-title":"Role of La Crosse virus glycoproteins in attachment of virus to host cells","volume":"181","author":"Ludwig","year":"1991","journal-title":"Virology."},{"key":"10.1016\/j.virusres.2025.199676_bib0037","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0168-1702(99)00070-2","article-title":"Murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses: a system for analysis of cell tropism","volume":"64","author":"Ma","year":"1999","journal-title":"Virus. Res."},{"key":"10.1016\/j.virusres.2025.199676_bib0004","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1056\/NEJM200103153441103","article-title":"La Crosse encephalitis in children","volume":"344","author":"McJunkin","year":"2001","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.virusres.2025.199676_bib0028","doi-asserted-by":"crossref","first-page":"7420","DOI":"10.1128\/JVI.72.9.7420-7427.1998","article-title":"Measles virus spread and pathogenesis in genetically modified mice","volume":"72","author":"Mrkic","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0022","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1863-2378.2011.01433.x","article-title":"La Crosse encephalitis surveillance using single versus paired serologic testing","volume":"59","author":"Murphree","year":"2012","journal-title":"Zoonoses. Public Health"},{"key":"10.1016\/j.virusres.2025.199676_bib0016","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1128\/jvi.19.3.985-997.1976","article-title":"Structural proteins of La Crosse virus","volume":"19","author":"Obijeski","year":"1976","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0020","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.1128\/jvi.69.6.3475-3481.1995","article-title":"Protection from La Crosse virus encephalitis with recombinant glycoproteins: role of neutralizing anti-G1 antibodies","volume":"69","author":"Pekosz","year":"1995","journal-title":"J. Virol."},{"key":"10.1016\/j.virusres.2025.199676_bib0035","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.micinf.2018.02.008","article-title":"Zika virus: lessons learned in Brazil","volume":"20","author":"Proenca-Modena","year":"2018","journal-title":"Microbes. Infect."},{"key":"10.1016\/j.virusres.2025.199676_bib0012","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/1743-422X-5-164","article-title":"Potential for La Crosse virus segment reassortment in nature","volume":"5","author":"Reese","year":"2008","journal-title":"Virol. J."},{"key":"10.1016\/j.virusres.2025.199676_bib0029","doi-asserted-by":"crossref","first-page":"7755","DOI":"10.1038\/s41598-019-44210-6","article-title":"Vesicular stomatitis virus-based vaccine protects mice against Crimean-Congo hemorrhagic fever","volume":"9","author":"Rodriguez","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.virusres.2025.199676_bib0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0237322","article-title":"Trout Fryxell RT. Spatial-temporal clusters of host-seeking Aedes albopictus, Aedes japonicus, and Aedes triseriatus collections in a La Crosse virus endemic county (Knox County, Tennessee, USA)","volume":"15","author":"Rowe","year":"2020","journal-title":"PLoS. One"},{"key":"10.1016\/j.virusres.2025.199676_bib0021","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1089\/10430349950017653","article-title":"DNA-based vaccine against La Crosse virus: protective immune response mediated by neutralizing antibodies and CD4+ T cells","volume":"10","author":"Schuh","year":"1999","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.virusres.2025.199676_bib0034","first-page":"59","article-title":"Development and validation of a multiplex microsphere immunoassay using dried blood spots for SARS-CoV-2 seroprevalence: application in first responders in Colorado, USA","author":"Schultz","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.virusres.2025.199676_bib0041","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2204706119","article-title":"Oropouche orthobunyavirus infection is mediated by the cellular host factor Lrp1","volume":"119","author":"Schwarz","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U S. A"},{"key":"10.1016\/j.virusres.2025.199676_bib0018","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.virol.2010.04.012","article-title":"La Crosse virus (LACV) Gc fusion peptide mutants have impaired growth and fusion phenotypes, but remain neurotoxic","volume":"404","author":"Soldan","year":"2010","journal-title":"Virology."},{"key":"10.1016\/j.virusres.2025.199676_bib0024","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S1535-6108(03)00241-1","article-title":"VSV strains with defects in their ability to shutdown innate immunity are potent systemic anti-cancer agents","volume":"4","author":"Stojdl","year":"2003","journal-title":"Cancer Cell"},{"key":"10.1016\/j.virusres.2025.199676_bib0039","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1080\/21645515.2018.1473698","article-title":"The vesicular stomatitis virus-based Ebola virus vaccine: from concept to clinical trials","volume":"14","author":"Suder","year":"2018","journal-title":"Hum. Vaccin. ImmunOther"},{"key":"10.1016\/j.virusres.2025.199676_bib0006","doi-asserted-by":"crossref","first-page":"807","DOI":"10.4269\/ajtmh.21-0294","article-title":"La Crosse virus disease in the United States, 2003-2019","volume":"105","author":"Vahey","year":"2021","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.virusres.2025.199676_bib0015","first-page":"10","article-title":"La Crosse Virus Shows Strain-Specific Differences in Pathogenesis","author":"Wilson","year":"2021","journal-title":"Pathogens"}],"container-title":["Virus Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170225001546?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168170225001546?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:00:49Z","timestamp":1773921649000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168170225001546"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":41,"alternative-id":["S0168170225001546"],"URL":"https:\/\/doi.org\/10.1016\/j.virusres.2025.199676","relation":{},"ISSN":["0168-1702"],"issn-type":[{"value":"0168-1702","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Replication competent La Crosse virus pseudotyped VSV vector system as vaccine and serological diagnostic reagent","name":"articletitle","label":"Article Title"},{"value":"Virus Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.virusres.2025.199676","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Mayo Clinic for Medical Education and Research. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"199676"}}