{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T12:52:16Z","timestamp":1793019136053,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002803","name":"Fondazione Cariplo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002803","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007211","name":"Istituto Nanoscienze, Consiglio Nazionale delle Ricerche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007211","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vascular Pharmacology"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1016\/j.vph.2020.106678","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T20:16:07Z","timestamp":1585340167000},"page":"106678","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["Simulated hyperglycemia impairs insulin signaling in endothelial cells through a hyperosmolar mechanism"],"prefix":"10.1016","volume":"130","author":[{"given":"Rosalinda","family":"Madonna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damiana","family":"Pieragostino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Confalone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Cicalini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Minnucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirco","family":"Zucchelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piero","family":"Del Boccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaele","family":"De Caterina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vph.2020.106678_bb0005","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1097\/00041433-199608000-00008","article-title":"Insulin resistance and coronary heart disease","volume":"7","author":"Laakso","year":"1996","journal-title":"Curr. Opin. Lipidol."},{"key":"10.1016\/j.vph.2020.106678_bb0010","doi-asserted-by":"crossref","first-page":"667","DOI":"10.2337\/diab.37.6.667","article-title":"Lilly lecture 1987. The triumvirate: beta-cell, muscle, liver. A collusion responsible for NIDDM","volume":"37","author":"DeFronzo","year":"1988","journal-title":"Diabetes"},{"key":"10.1016\/j.vph.2020.106678_bb0015","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1210\/en.2017-00312","article-title":"Combined Hyperglycemia- and Hyperinsulinemia-induced insulin resistance in adipocytes is associated with dual Signaling defects mediated by PKC-zeta","volume":"159","author":"Lu","year":"2018","journal-title":"Endocrinology"},{"key":"10.1016\/j.vph.2020.106678_bb0020","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1074\/jbc.M103728200","article-title":"Inhibition of phosphatidylinositol 3-kinase enhances mitogenic actions of insulin in endothelial cells","volume":"277","author":"Montagnani","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vph.2020.106678_bb0025","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1007\/s00125-004-1330-x","article-title":"Insulin enhances vascular cell adhesion molecule-1 expression in human cultured endothelial cells through a pro-atherogenic pathway mediated by p38 mitogen-activated protein-kinase","volume":"47","author":"Madonna","year":"2004","journal-title":"Diabetologia"},{"key":"10.1016\/j.vph.2020.106678_bb0030","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1515\/BC.2001.133","article-title":"Insulin resistance induced by loop diuretics and hyperosmolarity in perfused rat liver","volume":"382","author":"Schliess","year":"2001","journal-title":"Biol. Chem."},{"key":"10.1016\/j.vph.2020.106678_bb0035","series-title":"Diabetic Ketoacidosis and Hyperosmolar Hyperglycemic Syndrome","author":"BC L, Inzucchi S.","year":"2014"},{"key":"10.1016\/j.vph.2020.106678_bb0040","doi-asserted-by":"crossref","first-page":"577","DOI":"10.2337\/diab.28.6.577","article-title":"Severe hyperglycemia: effects of rehydration on endocrine derangements and blood glucose concentration","volume":"28","author":"Waldhausl","year":"1979","journal-title":"Diabetes"},{"key":"10.1016\/j.vph.2020.106678_bb0045","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.2337\/diab.32.11.1028","article-title":"Impairment of insulin-mediated glucose metabolism by hyperosmolality in man","volume":"32","author":"Bratusch-Marrain","year":"1983","journal-title":"Diabetes"},{"key":"10.1016\/j.vph.2020.106678_bb0050","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1177\/039463201002300309","article-title":"NA+\/H+ exchanger 1- and aquaporin-1-dependent hyperosmolarity changes decrease nitric oxide production and induce VCAM-1 expression in endothelial cells exposed to high glucose","volume":"23","author":"Madonna","year":"2010","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"10.1016\/j.vph.2020.106678_bb0055","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s12933-016-0342-4","article-title":"High glucose-induced hyperosmolarity contributes to COX-2 expression and angiogenesis: implications for diabetic retinopathy","volume":"15","author":"Madonna","year":"2016","journal-title":"Cardiovasc. Diabetol."},{"key":"10.1016\/j.vph.2020.106678_bb0060","first-page":"2266","article-title":"High glucose-induced hyperosmolarity impacts proliferation, cytoskeleton remodeling and migration of human induced pluripotent stem cells via aquaporin-1","volume":"2014","author":"Madonna","year":"1842","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.vph.2020.106678_bb0065","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1053\/j.ajkd.2013.04.007","article-title":"Effect of an L-carnitine-containing peritoneal dialysate on insulin sensitivity in patients treated with CAPD: a 4-month, prospective, multicenter randomized trial","volume":"62","author":"Bonomini","year":"2013","journal-title":"Am. J. Kidney Dis."},{"key":"10.1016\/j.vph.2020.106678_bb0070","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1039\/C4MB00700J","article-title":"An integrated metabolomics approach for the research of new cerebrospinal fluid biomarkers of multiple sclerosis","volume":"11","author":"Pieragostino","year":"2015","journal-title":"Mol. BioSyst."},{"issue":"Suppl. 2","key":"10.1016\/j.vph.2020.106678_bb0075","first-page":"s78","article-title":"Toward personalized hemodialysis by low molecular weight amino-containing compounds: future perspective of patient metabolic fingerprint","volume":"10","author":"Sirolli","year":"2012","journal-title":"Blood Transfus."},{"key":"10.1016\/j.vph.2020.106678_bb0080","doi-asserted-by":"crossref","first-page":"13503","DOI":"10.1074\/jbc.M411859200","article-title":"Simvastatin attenuates expression of cytokine-inducible nitric-oxide synthase in embryonic cardiac myoblasts","volume":"280","author":"Madonna","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vph.2020.106678_bb0085","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1152\/physrev.1998.78.1.247","article-title":"Functional significance of cell volume regulatory mechanisms","volume":"78","author":"Lang","year":"1998","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.vph.2020.106678_bb0090","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1091\/mbc.E17-01-0049","article-title":"Reciprocal regulation of eNOS and caveolin-1 functions in endothelial cells","volume":"29","author":"Chen","year":"2018","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.vph.2020.106678_bb0095","doi-asserted-by":"crossref","first-page":"3845","DOI":"10.1093\/nar\/gki701","article-title":"NFAT5 binds to the TNF promoter distinctly from NFATp, c, 3 and 4, and activates TNF transcription during hypertonic stress alone","volume":"33","author":"Esensten","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vph.2020.106678_bb0100","first-page":"F753","article-title":"Cis- and trans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity","volume":"274","author":"Miyakawa","year":"1998","journal-title":"Am. J. Phys."},{"key":"10.1016\/j.vph.2020.106678_bb0105","doi-asserted-by":"crossref","first-page":"29379","DOI":"10.1016\/S0021-9258(18)43888-4","article-title":"The tonicity-sensitive element that mediates increased transcription of the betaine transporter gene in response to hypertonic stress","volume":"269","author":"Takenaka","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vph.2020.106678_bb0110","doi-asserted-by":"crossref","first-page":"5253","DOI":"10.4049\/jimmunol.0901298","article-title":"Hypertonicity-induced expression of monocyte chemoattractant protein-1 through a novel cis-acting element and MAPK signaling pathways","volume":"184","author":"Kojima","year":"2010","journal-title":"J. Immunol."},{"key":"10.1016\/j.vph.2020.106678_bb0115","doi-asserted-by":"crossref","first-page":"15765","DOI":"10.1074\/jbc.M209980200","article-title":"Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene","volume":"278","author":"Umenishi","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.vph.2020.106678_bb0120","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1172\/JCI116337","article-title":"Defect in skeletal muscle phosphatidylinositol-3-kinase in obese insulin-resistant mice","volume":"91","author":"Heydrick","year":"1993","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.vph.2020.106678_bb0125","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.cmet.2009.02.002","article-title":"A branched-chain amino acid-related metabolic signature that differentiates obese and lean humans and contributes to insulin resistance","volume":"9","author":"Newgard","year":"2009","journal-title":"Cell Metab."},{"key":"10.1016\/j.vph.2020.106678_bb0130","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/1753-0407.12645","article-title":"Diabetes and branched-chain amino acids: what is the link?","volume":"10","author":"Bloomgarden","year":"2018","journal-title":"J. Diabetes"},{"key":"10.1016\/j.vph.2020.106678_bb0135","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/nrendo.2014.171","article-title":"Branched-chain amino acids in metabolic signalling and insulin resistance","volume":"10","author":"Lynch","year":"2014","journal-title":"Nat. Rev. Endocrinol."},{"key":"10.1016\/j.vph.2020.106678_bb0140","doi-asserted-by":"crossref","first-page":"10368","DOI":"10.1021\/acs.analchem.7b02283","article-title":"Comprehensive analysis of Acylcarnitine species in db\/db mouse using a novel method of high-resolution parallel reaction monitoring reveals widespread metabolic dysfunction induced by diabetes","volume":"89","author":"Xiang","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.vph.2020.106678_bb0145","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1194\/jlr.D061721","article-title":"An LC-MS\/MS method to quantify acylcarnitine species including isomeric and odd-numbered forms in plasma and tissues","volume":"56","author":"Giesbertz","year":"2015","journal-title":"J. Lipid Res."}],"container-title":["Vascular Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1537189119303945?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1537189119303945?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T01:18:38Z","timestamp":1761527918000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1537189119303945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":29,"alternative-id":["S1537189119303945"],"URL":"https:\/\/doi.org\/10.1016\/j.vph.2020.106678","relation":{},"ISSN":["1537-1891"],"issn-type":[{"value":"1537-1891","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simulated hyperglycemia impairs insulin signaling in endothelial cells through a hyperosmolar mechanism","name":"articletitle","label":"Article Title"},{"value":"Vascular Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vph.2020.106678","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"106678"}}