{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:46:26Z","timestamp":1791287186304,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004263","name":"FAPERGS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology: Regional Studies and Reports"],"published-print":{"date-parts":[[2021,4]]},"DOI":"10.1016\/j.vprsr.2021.100546","type":"journal-article","created":{"date-parts":[[2021,2,27]],"date-time":"2021-02-27T18:16:05Z","timestamp":1614449765000},"page":"100546","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Serological investigation of protozoan pathogens (Trypanosoma cruzi, Toxoplasma gondii and Neospora caninum) in opossums from southern Brazil"],"prefix":"10.1016","volume":"24","author":[{"given":"L.C.","family":"Zitelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Webster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.P.","family":"Morel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.A.","family":"Umeno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.C.","family":"Padilha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Dall'Agnol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.Z.","family":"Anicet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.M.","family":"Bandarra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Marsicano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Reck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vprsr.2021.100546_bb0005","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1590\/S0101-81752008000200008","article-title":"Use of space by neotropical marsupial Didelphis albiventris (Didelphimorphia: Didelphidae) in an urban forest fragment","volume":"25","author":"Almeida","year":"2008","journal-title":"Revista Brasileira de Zoologia"},{"key":"10.1016\/j.vprsr.2021.100546_bb0010","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0166-6851(99)00155-3","article-title":"The evolution of two Trypanosoma cruzi subgroups inferred from rRNA genes can be correlated with the interchange of American mammalian faunas in the Cenozoic and has implications to pathogenicity and host specificity","volume":"104","author":"Briones","year":"1999","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0015","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1089\/vbz.2009.0009","article-title":"Seroprevalence of Trypanosoma cruzi among eleven potential reservoir species from six states across the southern United States","volume":"10","author":"Brown","year":"2010","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"10.1016\/j.vprsr.2021.100546_bb0020","doi-asserted-by":"crossref","first-page":"964","DOI":"10.2460\/javma.1979.175.09.964","article-title":"Serologic survey for Toxoplasma gondii in wild animals in Florida","volume":"175","author":"Burridge","year":"1979","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1016\/j.vprsr.2021.100546_bb0025","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1076\/snfe.37.2.97.8582","article-title":"Food habits and seed dispersal by the white-eared opossum, Didelphis albiventris, in southern Brazil","volume":"37","author":"C\u00e1ceres","year":"2002","journal-title":"Stud. Neotropical Fauna Environ."},{"key":"10.1016\/j.vprsr.2021.100546_bb0030","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1590\/S1676-06032010000200004","article-title":"Potential seed dispersal by Didelphis albiventris (Marsupialia, Didelphidae) in highly disturbed environment","volume":"10","author":"Cantor","year":"2010","journal-title":"Biota Neotropica."},{"key":"10.1016\/j.vprsr.2021.100546_bb0045","series-title":"The IUCN Red List of Threatened Species","first-page":"1","article-title":"Didelphis albiventris","author":"Costa","year":"2015"},{"key":"10.1016\/j.vprsr.2021.100546_bb0050","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.jcpa.2011.05.002","article-title":"Neosporosis in a captive Parma wallaby (Macropus parma)","volume":"146","author":"Cronstedt-Fell","year":"2012","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2018.02108","article-title":"Prevalence, risk factors, and genotypes of Toxoplasma gondii in food animals and humans (2000\u20132017) from China","volume":"9","author":"Dong","year":"2018","journal-title":"Front. Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.vprsr.2021.100546_bb0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-4017(96)01035-7","article-title":"A review of Neospora caninum and neosporosis","volume":"67","author":"Dubey","year":"1996","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0070","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1645\/GE-709R.1","article-title":"Serologic survey for Toxoplasma gondii and Neospora caninum in the common Brushtail possum (Trichosurus Vulpecula) from urban Sydney, Australia","volume":"92","author":"Eymann","year":"2006","journal-title":"J. Parasitol."},{"issue":"1\u20132","key":"10.1016\/j.vprsr.2021.100546_bb0075","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1590\/S0074-02761980000100010","article-title":"Preval\u00eancia da infec\u00e7\u00e3o pelo Toxoplasma gondii em animais dom\u00e9sticos, silvestres e grupamentos humanos da Amaz\u00f4nia [Prevalence of Toxoplasma gondii infection in domestic and wild animals, and human groups of the Amazonas region]","volume":"75","author":"Ferraroni","year":"1980","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"2","key":"10.1016\/j.vprsr.2021.100546_bb3000","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1674\/0003-0031-175.2.155","article-title":"Habitat dynamics of the Virginia opossum in a highly urban landscape","volume":"175","author":"Fidino","year":"2016","journal-title":"The American Midland Naturalist"},{"key":"10.1016\/j.vprsr.2021.100546_bb0080","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.vetpar.2011.02.005","article-title":"Prevalence of antibodies against Toxoplasma gondii among Brazilian white-eared opossums (Didelphis albiventris)","volume":"179","author":"Fornazari","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0085","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1590\/S1984-29612015045","article-title":"Toxoplasma gondii antibodies in wild rodents and marsupials from the Atlantic Forest, state of S\u00e3o Paulo, Brazil","volume":"24","author":"Gennari","year":"2015","journal-title":"Braz. J. Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0090","first-page":"259","article-title":"Species or local environment, what determines the infection of rodents by Toxoplasma gondii?","volume":"141","author":"Gotteland","year":"2013","journal-title":"Cambri. Univ. Press"},{"key":"10.1016\/j.vprsr.2021.100546_bb0095","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.mambio.2008.03.003","article-title":"Environmental change and marsupial assemblages in Holocene successions of southern Brazil","volume":"74","author":"Hadler","year":"2009","journal-title":"Mamm. Biol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0100","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.trstmh.2004.07.006","article-title":"Trypanosoma cruzi infection in wild mammals of the National Park \u2018Serra da Capivara\u2019 and its surroundings (Piau\u00ed, Brazil), an area endemic for Chagas disease","volume":"99","author":"Herrera","year":"2005","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.vprsr.2021.100546_bb0105","doi-asserted-by":"crossref","first-page":"1","DOI":"10.11606\/issn.1678-4456.bjvras.2016.110381","article-title":"Occurrence of anti-Toxoplasma gondii, Neospora caninum and Leptospira spp. antibodies in opossums (Didelphis spp.) in S\u00e3o Paulo state, Brazil","volume":"53","author":"Horta","year":"2016","journal-title":"Braz. J. Vet. Res. Anim. Sci."},{"key":"10.1016\/j.vprsr.2021.100546_bb0110","first-page":"75","article-title":"Detection of anti-toxoplasma gondii antibodies in small wild mammals from preserved and non-preserved areas in the Caatinga biome, a semi-arid region of Northeast Brazil","volume":"14","author":"Horta","year":"2018","journal-title":"Vet. Parasitol.: Reg. Stud. Rep."},{"key":"10.1016\/j.vprsr.2021.100546_bb0115","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.actatropica.2012.07.009","article-title":"Leptospira borgpetersenii from free-living white-eared opossum (Didelphis albiventris): first isolation in Brazil","volume":"124","author":"Jorge","year":"2012","journal-title":"Acta Trop."},{"key":"10.1016\/j.vprsr.2021.100546_bb0120","series-title":"Origin and Evolution of Mammals","first-page":"285","author":"Kemp","year":"2005"},{"key":"10.1016\/j.vprsr.2021.100546_bb0125","series-title":"Australasian Marsupials and Monotremes: An Action Plan for their Conservation","author":"Kennedy","year":"2005"},{"key":"10.1016\/j.vprsr.2021.100546_bb0130","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1297-9716-42-75","article-title":"Extensive production of Neospora caninum tissue cysts in a carnivorous marsupial succumbing to experimental neosporosis","volume":"42","author":"King","year":"2011","journal-title":"Vet. Res."},{"key":"10.1016\/j.vprsr.2021.100546_bb0135","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1590\/0074-02760140400","article-title":"Infection with Trypanosoma cruzi TcII and TcI in free-ranging population of lion tamarins (Leontopithecus spp): an 11-year follow-up","volume":"110","author":"Lisboa","year":"2015","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.vprsr.2021.100546_bb0140","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.mambio.2009.06.004","article-title":"Feeding ecology of the marsupial Philander frenatus in a fragmented landscape in southeastern Brazil","volume":"75","author":"Macedo","year":"2010","journal-title":"Mamm. Biol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0145","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1590\/S1984-29612016028","article-title":"Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of S\u00e3o Paulo, Brazil","volume":"25","author":"Melo","year":"2016","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.vprsr.2021.100546_bb0150","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1645\/GE-800R.1","article-title":"Prevalence of agglutinating antibodies to Toxoplasma gondii in striped skunks (Mephitis mephitis), opossums (Didelphis virginiana), and raccoons (Procyon lotor) from Connecticut","volume":"92","author":"Mitchell","year":"2006","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0155","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1590\/S0036-46652003000300006","article-title":"Incidence of congenital toxoplasmosis in southern Brazil: a prospective study","volume":"45","author":"Mozzatto","year":"2003","journal-title":"Rev. Inst. Med. Trop."},{"key":"10.1016\/j.vprsr.2021.100546_bb0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1051\/vetres\/2009009","article-title":"Trypanosoma cruzi: adaptation to its vectors and its hosts","volume":"40","author":"Noireau","year":"2009","journal-title":"Vet. Res."},{"key":"10.1016\/j.vprsr.2021.100546_bb0165","first-page":"1","article-title":"Infection by Leishmania spp. in free-ranging opossums (Didelphis albiventris) in an environmentally protected area inhabited by humans in southeastern Brazil","volume":"20","author":"Paiz","year":"2016","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"10.1016\/j.vprsr.2021.100546_bb0170","doi-asserted-by":"crossref","first-page":"125","DOI":"10.4269\/ajtmh.2001.65.125","article-title":"Probability of Trypanosoma cruzi transmission by Triatoma infestans (Hemiptera: Reduviidae) to the opossum Didelphis albiventris (Marsupialia: Didelphidae)","volume":"65","author":"Rabinovich","year":"2001","journal-title":"Am.J. Trop. Med. Hyg."},{"key":"10.1016\/j.vprsr.2021.100546_bb0175","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1017\/S0031182012001539","article-title":"Trypanosoma cruzi TcI and TcII transmission among wild carnivores, small mammals and dogs in a conservation unit and surrounding areas, Brazil","volume":"140","author":"Rocha","year":"2013","journal-title":"Parasitology."},{"key":"10.1016\/j.vprsr.2021.100546_bb0180","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.vetpar.2012.11.028","article-title":"Trypanosoma cruzi among wild and domestic mammals in different areas of the Abaetetuba municipality (Par\u00e1 state, Brazil), an endemic Chagas disease transmission area","volume":"193","author":"Roque","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0185","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.vetpar.2012.12.027","article-title":"Latitudinal variability in the seroprevalence of antibodies against Toxoplasma gondii in non-migrant and Arctic migratory geese","volume":"194","author":"Sandstr\u00f6m","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0190","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1644\/11-MAMM-A-349.1","article-title":"Size and shape in cranial evolution of 2 marsupial genera: Didelphis and Philander (Didelphimorphia, Didelphidae)","volume":"94","author":"Sebasti\u00e3o","year":"2013","journal-title":"J. Mammal."},{"key":"10.1016\/j.vprsr.2021.100546_bb0195","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1645\/GE-2855.1","article-title":"Seroprevalence of Toxoplasma gondii in wild marsupials and rodents from the Atlantic Forest of Pernambuco state, northeastern region, Brazil","volume":"99","author":"Siqueira","year":"2013","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0200","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1590\/S0074-02761996000400002","article-title":"Trypanosoma cruzi in the anal glands of urban opossums. I- isolation and experimental infections","volume":"91","author":"Urdaneta-Morales","year":"1996","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.vprsr.2021.100546_bb0205","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1017\/S003118200700323X","article-title":"Effects of habitat fragmentation on wild mammal infection by Trypanosoma cruzi","volume":"134","author":"Vaz","year":"2007","journal-title":"Parasitol. Univ. Press."},{"key":"10.1016\/j.vprsr.2021.100546_bb0210","first-page":"38289","article-title":"Infection by Trypanosoma cruzi in mammals in Yucatan, Mexico: a serological and parasitological study","author":"Vel\u00e1zquez","year":"1996","journal-title":"Rev. Inst. Trop."},{"key":"10.1016\/j.vprsr.2021.100546_bb0215","first-page":"87","article-title":"Pathological alterations and prevalence of Trypanosoma cruzi in opossums from western Mexico","volume":"40","author":"Villagr\u00e1n","year":"2011","journal-title":"Bol. Mal. Salud Amb."},{"key":"10.1016\/j.vprsr.2021.100546_bb0220","doi-asserted-by":"crossref","first-page":"39","DOI":"10.4172\/1948-5948.1000097","article-title":"A high sensitive nested PCR for Toxoplasma gondii detection in animal and food samples","volume":"5","author":"Vitale","year":"2013","journal-title":"J. Microb. Biochem. Technol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0225","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1645\/GE-83R","article-title":"Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in the south American opossum (Didelphis marsupialis) from the City of S\u00e3o Paulo, Brazil","volume":"89","author":"Yai","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vprsr.2021.100546_bb0230","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ijpara.2004.10.024","article-title":"Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids","volume":"35","author":"Yeo","year":"2005","journal-title":"Elsevier \u2013 Int. J. Parasit."}],"container-title":["Veterinary Parasitology: Regional Studies and Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405939021000174?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405939021000174?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:19:27Z","timestamp":1762517967000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405939021000174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":44,"alternative-id":["S2405939021000174"],"URL":"https:\/\/doi.org\/10.1016\/j.vprsr.2021.100546","relation":{},"ISSN":["2405-9390"],"issn-type":[{"value":"2405-9390","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Serological investigation of protozoan pathogens (Trypanosoma cruzi, Toxoplasma gondii and Neospora caninum) in opossums from southern Brazil","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology: Regional Studies and Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vprsr.2021.100546","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"100546"}}