{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T15:14:53Z","timestamp":1781190893435,"version":"3.54.1"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cientifico e Tecnologico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Parasitology: Regional Studies and Reports"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.vprsr.2026.101495","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:50:15Z","timestamp":1777942215000},"page":"101495","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Viability and genetic diversity of Toxoplasma gondii in retail pork from a Brazilian region known for waterborne toxoplasmosis"],"prefix":"10.1016","volume":"72","author":[{"given":"Amanda Luc\u00eda","family":"Jim\u00e9nez-Sanz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwards","family":"Fraz\u00e3o-Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Carlos Rodrigues","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regiane de F\u00e1tima","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira Salim Mello","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.vprsr.2026.101495_bb0005","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1086\/597477","article-title":"Genotype of 88 Toxoplasma gondii isolates associated with toxoplasmosis in immunocompromised patients and correlation with clinical findings","volume":"199","author":"Ajzenberg","year":"2010","journal-title":"J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.vprsr.2026.101495_bb0010","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3201\/eid0901.020160","article-title":"Highly endemic, waterborne toxoplasmosis in North Rio de Janeiro state, Brazil","volume":"9","author":"Bahia-Oliveira","year":"2003","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.vprsr.2026.101495_bb0015","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1590\/S0001-37652007000100013","article-title":"High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil","volume":"79","author":"Belfort-Neto","year":"2007","journal-title":"An. Acad. Bras. Cienc."},{"issue":"2\u20134","key":"10.1016\/j.vprsr.2026.101495_bb0020","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.vetpar.2012.04.036","article-title":"Genetic characterization of Toxoplasma gondii isolates from pigs intended for human consumption in Brazil","volume":"189","author":"Bezerra","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2026.101495_bb0025","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1186\/s13071-023-06080-w","article-title":"Genetic diversity of toxoplasma gondii in South America: occurrence, immunity, and fate of infection","volume":"16","author":"Brito","year":"2023","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.vprsr.2026.101495_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2020.104590","article-title":"A simple method to generate PCR-RFLP typing profiles from DNA sequences in toxoplasma gondii","volume":"85","author":"Castro","year":"2020","journal-title":"Infect. Genet. Evol."},{"issue":"8","key":"10.1016\/j.vprsr.2026.101495_bb0035","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1007\/s11250-014-0667-5","article-title":"Occurrence and risk factors associated to Toxoplasma gondii infection in sheep from Rio de Janeiro","volume":"46","author":"Cosendey-KezenLeite","year":"2014","journal-title":"Brazil. Trop. Anim. Health Prod."},{"issue":"2","key":"10.1016\/j.vprsr.2026.101495_bb0040","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1645\/0022-3395(2003)089[0394:POTGIC]2.0.CO;2","article-title":"Prevalence of Toxoplasma gondii in chickens from an area in Southern Brazil highly endemic to humans","volume":"89","author":"da Silva","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vprsr.2026.101495_bb0045","doi-asserted-by":"crossref","first-page":"910","DOI":"10.2460\/ajvr.1988.49.06.910","article-title":"Long-term persistence of toxoplasma gondii in tissues of pigs inoculated with T. Gondii oocysts and effect of freezing on viability of tissue cysts in pork","volume":"49","author":"Dubey","year":"1988","journal-title":"Am. J. Vet. Res."},{"issue":"1","key":"10.1016\/j.vprsr.2026.101495_bb0050","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0304-4017(97)00135-0","article-title":"Refinement of pepsin digestion method for isolation of toxoplasma gondii from infected tissues","volume":"74","author":"Dubey","year":"1998","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.vprsr.2026.101495_bb0055","series-title":"Toxoplasmosis of Animals and Humans","author":"Dubey","year":"2022"},{"issue":"4","key":"10.1016\/j.vprsr.2026.101495_bb0060","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/j.2042-3306.1987.tb01426.x","article-title":"Serological responses of equids fed toxoplasma gondii oocysts","volume":"19","author":"Dubey","year":"1987","journal-title":"Equine Vet. J."},{"issue":"10","key":"10.1016\/j.vprsr.2026.101495_bb0065","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.2460\/ajvr.1984.45.10.1941","article-title":"Persistence of encysted toxoplasma gondii in tissues of pigs fed oocysts","volume":"45","author":"Dubey","year":"1984","journal-title":"Am. J. Vet. Res."},{"issue":"5","key":"10.1016\/j.vprsr.2026.101495_bb0070","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1645\/GE-683.1","article-title":"Prevalence of viable toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers","volume":"91","author":"Dubey","year":"2005","journal-title":"J. Parasitol."},{"issue":"1","key":"10.1016\/j.vprsr.2026.101495_bb0075","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1645\/GE-2457.1","article-title":"Anti-toxoplasma gondii antibodies in cattle and pigs in a highly endemic area for human toxoplasmosis in Brazil","volume":"97","author":"Fraz\u00e3o-Teixeira","year":"2011","journal-title":"J. Parasitol."},{"issue":"1\u20132","key":"10.1016\/j.vprsr.2026.101495_bb0080","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.vetpar.2010.09.030","article-title":"Multi-locus DNA sequencing of toxoplasma gondii isolated from Brazilian pigs identifies genetically divergent strains","volume":"175","author":"Fraz\u00e3o-Teixeira","year":"2011","journal-title":"Vet. Parasitol."},{"issue":"5","key":"10.1016\/j.vprsr.2026.101495_bb0085","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1645\/19-101","article-title":"Prevalence of anti-toxoplasma gondii antibodies in ratites from Brazil","volume":"105","author":"Gallo","year":"2019","journal-title":"J. Parasitol."},{"issue":"5","key":"10.1016\/j.vprsr.2026.101495_bb0090","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1086\/322800","article-title":"Unusual abundance of atypical strains associated with human ocular toxoplasmosis","volume":"184","author":"Grigg","year":"2001","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.vprsr.2026.101495_bb0095","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1093\/infdis\/172.6.1561","article-title":"Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease","volume":"172","author":"Howe","year":"1995","journal-title":"J. Infect. Dis."},{"issue":"9","key":"10.1016\/j.vprsr.2026.101495_bb0100","doi-asserted-by":"crossref","first-page":"687","DOI":"10.4315\/0362-028X-54.9.687","article-title":"Effect of freezing on infectivity of toxoplasma gondii tissue cysts in pork","volume":"54","author":"Kotula","year":"1991","journal-title":"J. Food Prot."},{"issue":"3","key":"10.1016\/j.vprsr.2026.101495_bb0105","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.parint.2014.03.001","article-title":"Identification and genetic characterization of a new Brazilian genotype of toxoplasma gondii from sheep intended for human consumption","volume":"63","author":"Maciel","year":"2014","journal-title":"Parasitol. Int."},{"issue":"5","key":"10.1016\/j.vprsr.2026.101495_bb0110","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.ijpara.2007.09.004","article-title":"Population structure and mouse virulence of toxoplasma gondii in Brazil","volume":"38","author":"Pena","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.vprsr.2026.101495_bb0115","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.meegid.2011.12.010","article-title":"Molecular genotyping of toxoplasma gondii from central and South America revealed high diversity within and between populations","volume":"12","author":"Rajendran","year":"2012","journal-title":"Infect. Genet. Evol."},{"issue":"12\u221213","key":"10.1016\/j.vprsr.2026.101495_bb0120","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. J. Parasitol."},{"issue":"1","key":"10.1016\/j.vprsr.2026.101495_bb0125","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1590\/S0102-09352007000100006","article-title":"Detection of toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs","volume":"59","author":"Tsutsui","year":"2007","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"issue":"1","key":"10.1016\/j.vprsr.2026.101495_bb0130","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1645\/GE-1746.1","article-title":"Isolate designation and characterization of toxoplasma gondii isolates from pigs in the United States","volume":"95","author":"Velmurugan","year":"2009","journal-title":"J. Parasitol."},{"key":"10.1016\/j.vprsr.2026.101495_bb0135","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.actatropica.2017.03.036","article-title":"Characterization of the zoonotic potential of toxoplasma gondii in horses from Rio de Janeiro state","volume":"171","author":"Venturi","year":"2017","journal-title":"Acta Trop."},{"issue":"3","key":"10.1016\/j.vprsr.2026.101495_bb0140","first-page":"227","article-title":"Avalia\u00e7\u00e3o da infec\u00e7\u00e3o experimental por Toxoplasma gondii em su\u00ednos por bioensaio em camundongos e PCR","volume":"40","author":"Yai","year":"2003","journal-title":"Braz. J. Vet. Res. Anim. Sci."}],"container-title":["Veterinary Parasitology: Regional Studies and Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405939026000754?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405939026000754?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T14:49:04Z","timestamp":1781189344000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405939026000754"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":28,"alternative-id":["S2405939026000754"],"URL":"https:\/\/doi.org\/10.1016\/j.vprsr.2026.101495","relation":{},"ISSN":["2405-9390"],"issn-type":[{"value":"2405-9390","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Viability and genetic diversity of Toxoplasma gondii in retail pork from a Brazilian region known for waterborne toxoplasmosis","name":"articletitle","label":"Article Title"},{"value":"Veterinary Parasitology: Regional Studies and Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vprsr.2026.101495","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"101495"}}