{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T04:09:06Z","timestamp":1788840546864,"version":"build-2803163510"},"reference-count":41,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water Research"],"published-print":{"date-parts":[[2004,5]]},"DOI":"10.1016\/j.watres.2004.03.006","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T11:04:15Z","timestamp":1084446255000},"page":"2529-2536","source":"Crossref","is-referenced-by-count":33,"title":["The use of isotopic and lipid analysis techniques linking toluene degradation to specific microorganisms: applications and limitations"],"prefix":"10.1016","volume":"38","author":[{"given":"Jiasong","family":"Fang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanh","family":"Lovanh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro J.J.","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.watres.2004.03.006_BIB1","doi-asserted-by":"crossref","first-page":"2981","DOI":"10.1128\/AEM.57.10.2981-2985.1991","article-title":"Substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries","volume":"57","author":"Alvarez","year":"1991","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10643389209388428","article-title":"Subsurface contaminant bioremediation engineering","volume":"22","author":"Tursman","year":"1992","journal-title":"Crit Rev Environ Control"},{"key":"10.1016\/j.watres.2004.03.006_BIB3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1128\/JB.124.1.7-13.1975","article-title":"Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2","volume":"124","author":"Worsey","year":"1975","journal-title":"J Bacteriol"},{"key":"10.1016\/j.watres.2004.03.006_BIB4","doi-asserted-by":"crossref","first-page":"14940","DOI":"10.1016\/S0021-9258(18)63793-7","article-title":"Toluene degradation by Pseudomonas putida F1","volume":"264","author":"Zylstra","year":"1989","journal-title":"J Biol Chem"},{"key":"10.1016\/j.watres.2004.03.006_BIB5","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1128\/AEM.55.6.1624-1629.1989","article-title":"Novel pathway of toluene catabolism in the trichloroethylene-degrading bacterium G4","volume":"55","author":"Shields","year":"1989","journal-title":"Appl and Environ Eng"},{"key":"10.1016\/j.watres.2004.03.006_BIB6","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/BF00058836","article-title":"The biodegradation of aromatic hydrocarbons by bacteria","volume":"1","author":"Smith","year":"1990","journal-title":"Biodegradation"},{"key":"10.1016\/j.watres.2004.03.006_BIB7","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1128\/jb.173.9.3017-3020.1991","article-title":"Separation and partial characterization of the enzymes of the toluene-4-monooxygenase catabolic pathway in Pseudomonas mendocina KR1","volume":"173","author":"Whited","year":"1991","journal-title":"J Bacteriol"},{"key":"10.1016\/j.watres.2004.03.006_BIB8","doi-asserted-by":"crossref","first-page":"3749","DOI":"10.1128\/jb.176.12.3749-3756.1994","article-title":"A novel toluene-3-monooxygenase pathway cloned from Pseudomonas pickettii PKO1","volume":"176","author":"Olsen","year":"1994","journal-title":"J Bacteriology"},{"key":"10.1016\/j.watres.2004.03.006_BIB9","doi-asserted-by":"crossref","first-page":"14066","DOI":"10.1021\/bi00043a012","article-title":"Purification and characterization of toluene-2-monooxygenase from Burkholderia cepacia G4","volume":"34","author":"Newman","year":"1995","journal-title":"Biochemistry"},{"key":"10.1016\/j.watres.2004.03.006_BIB10","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1472-765X.1996.tb00078.x","article-title":"Diversity of bacterial capabilities in utilizing alkylated benzenes and other aromatic compounds","volume":"23","author":"Lang","year":"1996","journal-title":"Lett Appl Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB11","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0168-1656(98)00166-7","article-title":"Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by a coculture of Pesudomonas putida and Pseudomonas fluorescene immobilized in a fibrous-bed reactor","volume":"67","author":"Shim","year":"1999","journal-title":"J Biotechnol"},{"key":"10.1016\/j.watres.2004.03.006_BIB12","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1007\/s002530000389","article-title":"Phospholipid compositional changes of five Pseudomonad archetypes grown with and without toluene","volume":"54","author":"Fang","year":"2000","journal-title":"Appl Microbiol Biotechnol,"},{"key":"10.1016\/j.watres.2004.03.006_BIB13","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1128\/AEM.62.3.974-978.1996","article-title":"Benzylfumaric, benzylmaleic, and Z- and E-phenyltaconic acids","volume":"62","author":"Migaud","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB14","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1128\/AEM.65.12.5403-5408.1999","article-title":"Linking toluene degradation with specific microbial populations in soil","volume":"65","author":"Hanson","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB15","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1046\/j.1462-2920.1999.00050.x","article-title":"13C\/13C stable isotope fractionation of toluene by anaerobic degradation","volume":"1","author":"Meckenstock","year":"1999","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB16","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1046\/j.1462-2920.1999.00023.x","article-title":"Towards elucidation of microbial community metabolic pathways","volume":"1","author":"Pelz","year":"1999","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB17","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1007\/s002030100259","article-title":"Use of isotopic and molecular techniques to link toluene degradation in denitrifying aquifer microorganisms to specific microbial populations","volume":"175","author":"Pelz","year":"2001","journal-title":"Arch Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB18","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/S0146-6380(99)00064-9","article-title":"Contrasting carbon isotope fractionation during biodegradation of trichloroethylene and toluene","volume":"30","author":"Sherwood-Loller","year":"1999","journal-title":"Org Geochem"},{"key":"10.1016\/j.watres.2004.03.006_BIB19","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0146-6380(99)00147-3","article-title":"Anaerobic degradation and carbon isotopic fractionation of alkylbenzenes in crude oil by sulphate-reducing bacteria","volume":"31","author":"Wilks","year":"2000","journal-title":"Org Geochem"},{"key":"10.1016\/j.watres.2004.03.006_BIB20","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.1128\/AEM.67.10.4842-4849.2001","article-title":"Stable hydrogen and carbon isotope fractionation during microbial toluene degradation","volume":"67","author":"Morasch","year":"2001","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB21","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1016\/S0045-6535(97)10193-X","article-title":"The use of 13C-labeled polycyclic aromatic hydrocarbons for the analysis of their transformation in soils","volume":"36","author":"Richnow","year":"1998","journal-title":"Chemosphere"},{"key":"10.1016\/j.watres.2004.03.006_BIB22","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1021\/es990889n","article-title":"Efficacy of bacterial bioremediation","volume":"34","author":"Rodgers","year":"2000","journal-title":"Environ Sci Technol"},{"key":"10.1016\/j.watres.2004.03.006_BIB23","doi-asserted-by":"crossref","first-page":"4202","DOI":"10.1128\/AEM.64.11.4202-4209.1998","article-title":"Ratios of carbon isotopes in microbial lipids as an indicator of substrate usage","volume":"64","author":"Abraham","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB24","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1038\/33900","article-title":"Direct linking of microbial populations to specific biogeochemical processes by 13C-labeling of biomarkers","volume":"392","author":"Boschker","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.watres.2004.03.006_BIB25","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1574-6941.2002.tb00987.x","article-title":"Field-scale 13C-labeling of phospholipid fatty acids (PLFA) and dissolved inorganic carbon","volume":"41","author":"Pombo","year":"2002","journal-title":"FEMS Microbiol Ecol"},{"key":"10.1016\/j.watres.2004.03.006_BIB26","series-title":"Methodology for biomass determination and microbial activities in sediments. ASTM STP 673","first-page":"87","article-title":"Lipid analysis of sediments for microbial biomass and community structure","author":"White","year":"1979"},{"key":"10.1016\/j.watres.2004.03.006_BIB27","series-title":"Molecular markers in Environ geochemistry","first-page":"65","article-title":"The use of aromatic acids and phospholipid ester-linked fatty acids for delineation of processes affecting an aquifer contaminated with JP-4 fuel","author":"Fang","year":"1997"},{"key":"10.1016\/j.watres.2004.03.006_BIB28","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0167-7012(98)00037-2","article-title":"Structural determination and quantitative analysis of phospholipids using liquid chromatography\/electrospray ionization\/mass spectrometry","volume":"33","author":"Fang","year":"1998","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.watres.2004.03.006_BIB29","doi-asserted-by":"crossref","first-page":"3566","DOI":"10.1128\/AEM.65.8.3566-3574.1999","article-title":"Microbial population changes during bioremediation of an experimental oil spill","volume":"65","author":"MacNaughton","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB30","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1128\/AEM.65.7.3056-3063.1999","article-title":"Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer","volume":"65","author":"Rooney-Varga","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB31","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1099\/00221287-43-2-159","article-title":"The aerobic pseudomonads","volume":"43","author":"Stanier","year":"1966","journal-title":"J Gen Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB32","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1021\/bi00732a008","article-title":"Initial reactions in the oxidation of ethylbenzene by Pseudomonas putida","volume":"12","author":"Gibson","year":"1973","journal-title":"Biochemistry"},{"key":"10.1016\/j.watres.2004.03.006_BIB33","unstructured":"Koch A. Growth Measurement. In: Murray RGE, Wood WA, Krieg NR, editors. Methods for general and molecular bacteriology. Washington DC: ASM; 1994. p. 249\u201376."},{"key":"10.1016\/j.watres.2004.03.006_BIB34","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0167-7012(96)00929-3","article-title":"The use of a classic lipid extraction method for simultaneous recovery of organic pollutants and microbial lipids from sediments","volume":"27","author":"Fang","year":"1996","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.watres.2004.03.006_BIB35","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/BF01411414","article-title":"Double-bond location in monounsaturated fatty acids by dimethyl disulfide derivatization and mass spectrometry","volume":"11","author":"Dunkleblum","year":"1985","journal-title":"J Chem Ecol"},{"key":"10.1016\/j.watres.2004.03.006_BIB36","series-title":"Principal component analysis","author":"Jolliffe","year":"1986"},{"key":"10.1016\/j.watres.2004.03.006_BIB37","doi-asserted-by":"crossref","first-page":"5568","DOI":"10.1016\/S0021-9258(19)83814-0","article-title":"Biosynthetic control of the natural abundance of carbon at specific positions within fatty acids in Saccharomeces cerevisiae","volume":"257","author":"Monson","year":"1982","journal-title":"J Biol Chem"},{"key":"10.1016\/j.watres.2004.03.006_BIB38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-6968.1985.tb01143.x","article-title":"Phospholipid ester-linked fatty acid profile as reproducible assays for changes in prokaryotic community structure of estuarine marine sediments","volume":"31","author":"Guckert","year":"1985","journal-title":"FEMS Microbiol Ecol"},{"key":"10.1016\/j.watres.2004.03.006_BIB39","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/S0723-2020(96)80025-7","article-title":"Fatty acid content in whole cell hydrolysates and phospholipid fractions of pseudomonads","volume":"19","author":"Vancanneyt","year":"1996","journal-title":"Syst Appl Microbiol"},{"key":"10.1016\/j.watres.2004.03.006_BIB40","series-title":"Manual of environmental microbiology","first-page":"91","article-title":"Biomass measurements","author":"White","year":"1991"},{"key":"10.1016\/j.watres.2004.03.006_BIB41","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1574-6941.2001.tb00792.x","article-title":"Bacterial populations and processes involved in acetate and propionate consumption in anoxic brackish sediment","volume":"35","author":"Boschker","year":"2001","journal-title":"FEMS Microbiol Ecol"}],"container-title":["Water Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0043135404001290?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0043135404001290?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T14:00:45Z","timestamp":1623938445000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0043135404001290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":41,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["S0043135404001290"],"URL":"https:\/\/doi.org\/10.1016\/j.watres.2004.03.006","relation":{},"ISSN":["0043-1354"],"issn-type":[{"value":"0043-1354","type":"print"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}