{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T20:58:40Z","timestamp":1793134720788,"version":"4.5.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Water Research"],"published-print":{"date-parts":[[2017,9]]},"DOI":"10.1016\/j.watres.2017.05.039","type":"journal-article","created":{"date-parts":[[2017,5,20]],"date-time":"2017-05-20T03:32:47Z","timestamp":1495251167000},"page":"240-247","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":62,"special_numbering":"C","title":["Molecular characterization of human adenoviruses in urban wastewaters using next generation and Sanger sequencing"],"prefix":"10.1016","volume":"121","author":[{"given":"M.","family":"Iaconelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Valdazo-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Equestre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.R.","family":"Ciccaglione","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Marcantonio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Della Libera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2657-100X","authenticated-orcid":false,"given":"G.","family":"La Rosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.watres.2017.05.039_bib1","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1007\/BF01690880","article-title":"Gastrointestinal adenovirus infections in a tertiary referral centre in Saudi Arabia","volume":"14","author":"Akhter","year":"1995","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"1\u20132","key":"10.1016\/j.watres.2017.05.039_bib2","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jviromet.2009.02.004","article-title":"Comparison of methods for concentrating human adenoviruses, polyomavirus JC and noroviruses in source waters and drinking water using quantitative PCR","volume":"158","author":"Albinana-Gimenez","year":"2009","journal-title":"J.\u00a0Virol. Methods"},{"issue":"4","key":"10.1016\/j.watres.2017.05.039_bib3","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1472-765X.2012.03220.x","article-title":"Detection and molecular identification of human adenoviruses and enteroviruses in wastewater from Morocco","volume":"54","author":"Amdiouni","year":"2012","journal-title":"Lett. Appl. Microbiol."},{"issue":"Pt 6","key":"10.1016\/j.watres.2017.05.039_bib4","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1099\/jmm.0.072413-0","article-title":"High frequency of cultivable human subgroup F adenoviruses in stool samples from a paediatric population admitted to hospital with acute gastroenteritis","volume":"63","author":"Arcangeletti","year":"2014","journal-title":"J.\u00a0Med. Microbiol."},{"issue":"1","key":"10.1016\/j.watres.2017.05.039_bib5","first-page":"109","article-title":"When should adenoviral non-gonococcal urethritis be suspected? Two case reports","volume":"37","author":"Avolio","year":"2014","journal-title":"New Microbiol."},{"issue":"4","key":"10.1016\/j.watres.2017.05.039_bib6","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1021\/es305181x","article-title":"Identification of viral pathogen diversity in sewage sludge by metagenome analysis","volume":"47","author":"Bibby","year":"2013","journal-title":"Environ. Sci. Technol."},{"issue":"12","key":"10.1016\/j.watres.2017.05.039_bib7","doi-asserted-by":"crossref","first-page":"7894","DOI":"10.1128\/AEM.00965-06","article-title":"Quantification and stability of human adenoviruses and polyomavirus JCPyV in wastewater matrices","volume":"72","author":"Bofill-Mas","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib8","doi-asserted-by":"crossref","first-page":"239","DOI":"10.2166\/wst.2006.475","article-title":"Epidemiological surveillance of human enteric viruses by monitoring of different environmental matrices","volume":"54","author":"Carducci","year":"2006","journal-title":"Water Sci. Technol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib9","first-page":"329","article-title":"Microbiological and epidemiological aspects of rotavirus and enteric adenovirus infections in hospitalized children in Italy","volume":"31","author":"Carraturo","year":"2008","journal-title":"New Microbiol."},{"issue":"11","key":"10.1016\/j.watres.2017.05.039_bib10","doi-asserted-by":"crossref","first-page":"7426","DOI":"10.1128\/AEM.71.11.7426-7433.2005","article-title":"Real-time PCR quantification of human adenoviruses in urban rivers indicates genome prevalence but low infectivity","volume":"71","author":"Choi","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib11","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1080\/13550280600846633","article-title":"Acute meningoencephalitis caused by adenovirus serotype 26","volume":"12","author":"Dubberke","year":"2006","journal-title":"J.\u00a0Neurovirol."},{"issue":"6","key":"10.1016\/j.watres.2017.05.039_bib12","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1093\/milmed\/160.6.300","article-title":"Adenovirus vaccines in the U.S. military","volume":"160","author":"Gaydos","year":"1995","journal-title":"Mil. Med."},{"issue":"17","key":"10.1016\/j.watres.2017.05.039_bib13","doi-asserted-by":"crossref","first-page":"6750","DOI":"10.1016\/j.watres.2013.09.001","article-title":"Evaluation of human adenovirus and human polyomavirus as indicators of human sewage contamination in the aquatic environment","volume":"47","author":"Hewitt","year":"2013","journal-title":"Water Res."},{"key":"10.1016\/j.watres.2017.05.039_bib14","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1186\/1471-2164-10-557","article-title":"Unique sequence features of the Human adenovirus 31 complete genomic sequence are conserved in clinical isolates","volume":"10","author":"Hofmayer","year":"2009","journal-title":"BMC Genomics"},{"issue":"1","key":"10.1016\/j.watres.2017.05.039_bib15","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s12560-016-9263-3","article-title":"One-year surveillance of human enteric viruses in raw and treated wastewaters, downstream river waters, and drinking waters","volume":"9","author":"Iaconelli","year":"2016","journal-title":"Food Environ. Virol."},{"issue":"6","key":"10.1016\/j.watres.2017.05.039_bib16","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1128\/JCM.43.6.2575-2580.2005","article-title":"First reported outbreak of diarrhea due to adenovirus infection in a hematology unit for adults","volume":"43","author":"Jalal","year":"2005","journal-title":"J.\u00a0Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.watres.2017.05.039_bib17","doi-asserted-by":"crossref","first-page":"650","DOI":"10.2166\/wh.2009.088","article-title":"Evaluation of four cell lines for assay of infectious adenoviruses in water samples","volume":"7","author":"Jiang","year":"2009","journal-title":"J.\u00a0Water Health"},{"issue":"10","key":"10.1016\/j.watres.2017.05.039_bib18","doi-asserted-by":"crossref","first-page":"e26862","DOI":"10.1371\/journal.pone.0026862","article-title":"Evaluation of type-specific real-time PCR assays using the LightCycler and J.B.A.I.D.S. for detection of adenoviruses in species HAdV-C","volume":"6","author":"Jones","year":"2011","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.watres.2017.05.039_bib19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1086\/518442","article-title":"Molecular epidemiology of adenovirus type 4 infections in US military recruits in the postvaccination era (1997-2003)","volume":"196","author":"Kajon","year":"2007","journal-title":"J.\u00a0Infect. Dis."},{"issue":"6","key":"10.1016\/j.watres.2017.05.039_bib20","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1001\/archneur.1979.00500420094016","article-title":"Adenoviral meningoencephalitis in a patient with lead toxicity","volume":"36","author":"Kelsey","year":"1979","journal-title":"Arch. Neurol."},{"issue":"195","key":"10.1016\/j.watres.2017.05.039_bib21","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/1743-422X-8-195","article-title":"Molecular detection of multiple viral targets in untreated urban sewage from Greece","volume":"8","author":"Kokkinos","year":"2011","journal-title":"Virol. J."},{"issue":"5","key":"10.1016\/j.watres.2017.05.039_bib22","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1016\/j.watres.2009.10.039","article-title":"Assessment of human adenovirus removal in a full-scale membrane bioreactor treating municipal wastewater","volume":"44","author":"Kuo","year":"2010","journal-title":"Water Res."},{"issue":"4","key":"10.1016\/j.watres.2017.05.039_bib23","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1111\/jam.12907","article-title":"High prevalence of type 41 and high sequence diversity of partial hexon gene of human adenoviruses in municipal raw sewage and activated sludge","volume":"119","author":"Kuo","year":"2015","journal-title":"J.\u00a0Appl. Microbiol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib24","first-page":"177","article-title":"Molecular characterization of human adenoviruses isolated in Italy","volume":"29","author":"La Rosa","year":"2006","journal-title":"new Microbiol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib25","first-page":"266","article-title":"Quantitative real-time PCR of enteric viruses in influent and effluent samples from wastewater treatment plants in Italy","volume":"46","author":"La Rosa","year":"2010","journal-title":"Ann. Ist. Super. Sanit\u00e0"},{"issue":"Pt 2","key":"10.1016\/j.watres.2017.05.039_bib26","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1099\/vir.0.023176-0","article-title":"Molecular characterization of adenovirus from clinical samples through analysis of the hexon and fiber genes","volume":"92","author":"La Rosa","year":"2011","journal-title":"J.\u00a0general Virol."},{"key":"10.1016\/j.watres.2017.05.039_bib27","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1186\/1471-2334-14-419","article-title":"Surveillance of hepatitis A virus in urban sewages and comparison with cases notified in the course of an outbreak, Italy 2013","volume":"14","author":"La Rosa","year":"2014","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.watres.2017.05.039_bib28","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1155\/2015\/142912","article-title":"Genetic diversity of human adenovirus in children with acute gastroenteritis, Albania, 2013\u20132015","volume":"2015","author":"La Rosa","year":"2015","journal-title":"BioMed Res. Int."},{"issue":"6","key":"10.1016\/j.watres.2017.05.039_bib29","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/rmv.589","article-title":"Clinical features and treatment of adenovirus infections","volume":"18","author":"Lenaerts","year":"2008","journal-title":"Rev. Med. Virol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib30","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1128\/CMR.00116-13","article-title":"Adenovirus infections in immunocompetent and immunocompromised patients","volume":"27","author":"Lion","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"issue":"8","key":"10.1016\/j.watres.2017.05.039_bib31","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1007\/s00705-005-0722-7","article-title":"Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene","volume":"151","author":"Lu","year":"2006","journal-title":"Arch. Virol."},{"issue":"23","key":"10.1016\/j.watres.2017.05.039_bib32","doi-asserted-by":"crossref","first-page":"2890","DOI":"10.1016\/j.vaccine.2008.03.037","article-title":"A\u00a0double-blind, placebo-controlled study of the safety and immunogenicity of live, oral type 4 and type 7 adenovirus vaccines in adults","volume":"26","author":"Lyons","year":"2008","journal-title":"Vaccine"},{"issue":"24","key":"10.1016\/j.watres.2017.05.039_bib33","doi-asserted-by":"crossref","first-page":"15265","DOI":"10.1128\/JVI.79.24.15265-15276.2005","article-title":"Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy","volume":"79","author":"Madisch","year":"2005","journal-title":"J.\u00a0Virol."},{"issue":"1","key":"10.1016\/j.watres.2017.05.039_bib34","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.virol.2009.10.052","article-title":"Natural variants of human adenovirus type 3 provide evidence for relative genome stability across time and geographic space","volume":"397","author":"Mahadevan","year":"2010","journal-title":"Virology"},{"issue":"Pt 12","key":"10.1016\/j.watres.2017.05.039_bib35","doi-asserted-by":"crossref","first-page":"2770","DOI":"10.1099\/vir.0.034744-0","article-title":"Genomic characterization of a novel human adenovirus type 31 recombinant in the hexon gene","volume":"92","author":"Matsushima","year":"2011","journal-title":"J.\u00a0General Virol."},{"issue":"10","key":"10.1016\/j.watres.2017.05.039_bib36","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1086\/522970","article-title":"Abrupt emergence of diverse species B adenoviruses at US military recruit training centers","volume":"196","author":"Metzgar","year":"2007","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.watres.2017.05.039_bib37","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1186\/s12879-014-0666-1","article-title":"Prevalence and molecular characterisation of human adenovirus in diarrhoeic children in Tanzania; a case control study","volume":"14","author":"Moyo","year":"2014","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.watres.2017.05.039_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11270-007-9608-5","article-title":"Detection and quantification of human adenoviruses in surface waters by nested PCR, TaqMan real-time PCR and cell culture assays","volume":"191","author":"Muscillo","year":"2008","journal-title":"Water Air Soil Pollut."},{"issue":"4","key":"10.1016\/j.watres.2017.05.039_bib39","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s12560-013-9121-5","article-title":"GIV noroviruses in wastewaters and in stool specimens from hospitalized patients","volume":"5","author":"Muscillo","year":"2013","journal-title":"Food Environ. Virol."},{"issue":"2","key":"10.1016\/j.watres.2017.05.039_bib40","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s12560-015-9194-4","article-title":"Human adenovirus diversity in water samples using a next-generation amplicon sequencing approach","volume":"7","author":"Ogorzaly","year":"2015","journal-title":"Food Environ. Virol."},{"issue":"6","key":"10.1016\/j.watres.2017.05.039_bib41","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.3390\/ijerph7062620","article-title":"Inadequately treated wastewater as a source of human enteric viruses in the environment","volume":"7","author":"Okoh","year":"2010","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.watres.2017.05.039_bib42","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1743-422X-11-25","article-title":"High prevalence and diversity of species D adenoviruses (HAdV-D) in human populations of four Sub-Saharan countries","volume":"11","author":"Pauly","year":"2014","journal-title":"Virol. J."},{"issue":"5","key":"10.1016\/j.watres.2017.05.039_bib43","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1111\/j.1365-2672.2008.04128.x","article-title":"Presence and environmental circulation of enteric viruses in three different wastewater treatment plants","volume":"106","author":"Petrinca","year":"2009","journal-title":"J.\u00a0Appl. Microbiol."},{"issue":"9","key":"10.1016\/j.watres.2017.05.039_bib44","doi-asserted-by":"crossref","first-page":"3376","DOI":"10.1128\/AEM.64.9.3376-3382.1998","article-title":"Viral pollution in the environment and in shellfish: human adenovirus detection by PCR as an index of human viruses","volume":"64","author":"Pina","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.watres.2017.05.039_bib45","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1186\/1471-2164-9-213","article-title":"Genomic and bioinformatics analysis of human adenovirus type 37: new insights into corneal tropism","volume":"9","author":"Robinson","year":"2008","journal-title":"BMC Genomics"},{"issue":"Pt 1","key":"10.1016\/j.watres.2017.05.039_bib46","first-page":"1","article-title":"Adenoviruses: update on structure and function","volume":"90","author":"Russell","year":"2009","journal-title":"J.\u00a0General Virol."},{"key":"10.1016\/j.watres.2017.05.039_bib47","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.virol.2015.08.018","article-title":"Recombination of the epsilon determinant and corneal tropism: human adenovirus species D types 15, 29, 56, and 69","volume":"485","author":"Singh","year":"2015","journal-title":"Virology"},{"issue":"12","key":"10.1016\/j.watres.2017.05.039_bib48","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib49","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1590\/S1517-838246320140718","article-title":"Seasonal variation on the presence of adenoviruses in stools from non-diarrheic patients","volume":"46","author":"Vetter","year":"2015","journal-title":"Braz. J. Microbiol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib50","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/j.watres.2010.10.015","article-title":"Surveillance of adenoviruses and noroviruses in European recreational waters","volume":"45","author":"Wyn-Jones","year":"2011","journal-title":"Water Res."},{"issue":"24","key":"10.1016\/j.watres.2017.05.039_bib51","doi-asserted-by":"crossref","first-page":"7874","DOI":"10.1128\/AEM.01239-07","article-title":"Occurrence of human adenoviruses at two recreational beaches of the great lakes","volume":"73","author":"Xagoraraki","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.watres.2017.05.039_bib52","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/s12560-015-9182-8","article-title":"Development and evaluation of a loop-mediated isothermal amplification assay for the detection of adenovirus 40 and 41","volume":"7","author":"Ziros","year":"2015","journal-title":"Food Environ. Virol."}],"container-title":["Water Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0043135417304037?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0043135417304037?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T04:36:43Z","timestamp":1602131803000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0043135417304037"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":52,"alternative-id":["S0043135417304037"],"URL":"https:\/\/doi.org\/10.1016\/j.watres.2017.05.039","relation":{},"ISSN":["0043-1354"],"issn-type":[{"value":"0043-1354","type":"print"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular characterization of human adenoviruses in urban wastewaters using next generation and Sanger sequencing","name":"articletitle","label":"Article Title"},{"value":"Water Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.watres.2017.05.039","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2017 Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}