{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:55:03Z","timestamp":1790463303840,"version":"4.1.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T00:00:00Z","timestamp":1572825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Water Research"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.watres.2019.115246","type":"journal-article","created":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T09:49:29Z","timestamp":1572601769000},"page":"115246","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":74,"special_numbering":"C","title":["Monitoring of enterovirus diversity in wastewater by ultra-deep sequencing: An effective complementary tool for clinical enterovirus surveillance"],"prefix":"10.1016","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3489-049X","authenticated-orcid":false,"given":"Maxime","family":"Bisseux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Debroas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Mirand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Archimbaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Peigue-Lafeuille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Bailly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Henquell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.watres.2019.115246_bib1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s12560-014-9137-5","article-title":"Isolation and identification of enteroviruses from sewage and sewage-contaminated water in Lagos, Nigeria","volume":"6","author":"Adeniji","year":"2014","journal-title":"Food Environ. Virol."},{"issue":"46","key":"10.1016\/j.watres.2019.115246_bib2","doi-asserted-by":"crossref","first-page":"30402","DOI":"10.2807\/1560-7917.ES.2016.21.46.30402","article-title":"Severe paediatric conditions linked with EV-A71 and EV-D68, France, May to October 2016","volume":"21","author":"Antona","year":"2016","journal-title":"Euro Surveill."},{"issue":"2","key":"10.1016\/j.watres.2019.115246_bib3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s11262-012-0776-z","article-title":"Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines","volume":"45","author":"Apostol","year":"2012","journal-title":"Virus Genes"},{"key":"10.1016\/j.watres.2019.115246_bib4","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jcv.2017.05.017","article-title":"An enhanced Enterovirus surveillance system allows identification and characterization of rare and emerging respiratory enteroviruses in Denmark, 2015-16","volume":"93","author":"Barnadas","year":"2017","journal-title":"J.\u00a0Clin. Virol."},{"key":"10.1016\/j.watres.2019.115246_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.00558-17","article-title":"Polio and measles down the drain: environmental enterovirus surveillance in The Netherlands, 2005-2015","volume":"83","author":"Benschop","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"issue":"383","key":"10.1016\/j.watres.2019.115246_bib6","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaf6786","article-title":"Estimation of polio infection prevalence from environmental surveillance data","volume":"9","author":"Berchenko","year":"2017","journal-title":"Sci. Transl. Med."},{"issue":"9","key":"10.1016\/j.watres.2019.115246_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0024823","article-title":"Genetic relationship between cocirculating human enteroviruses species C","volume":"6","author":"Bessaud","year":"2011","journal-title":"PLoS One"},{"issue":"9","key":"10.1016\/j.watres.2019.115246_bib8","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1586\/14787210.2015.1058156","article-title":"Coxsackievirus A6: a new emerging pathogen causing hand, foot and mouth disease outbreaks worldwide","volume":"13","author":"Bian","year":"2015","journal-title":"Expert Rev. Anti Infect. Ther."},{"issue":"7","key":"10.1016\/j.watres.2019.115246_bib9","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2018.23.7.17-00237","article-title":"Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015","volume":"23","author":"Bisseux","year":"2018","journal-title":"Euro Surveill."},{"issue":"3","key":"10.1016\/j.watres.2019.115246_bib10","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00099-17","article-title":"Retrospective surveillance of wastewater to examine seasonal dynamics of enterovirus infections","volume":"2","author":"Brinkman","year":"2017","journal-title":"mSphere"},{"key":"10.1016\/j.watres.2019.115246_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.cmi.2017.03.016","article-title":"Outbreak of brainstem encephalitis associated with enterovirus-A71 in Catalonia, Spain (2016): a clinical observational study in a children\u2019s reference centre in Catalonia","author":"Casas-Alba","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"issue":"7","key":"10.1016\/j.watres.2019.115246_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0180608","article-title":"The role of supplementary environmental surveillance to complement acute flaccid paralysis surveillance for wild poliovirus in Pakistan - 2011-2013","volume":"12","author":"Cowger","year":"2017","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.watres.2019.115246_bib13","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s12560-018-9350-8","article-title":"Poliovirus and other enteroviruses from environmental surveillance in Italy, 2009-2015","volume":"10","author":"Delogu","year":"2018","journal-title":"Food Environ. Virol."},{"issue":"5","key":"10.1016\/j.watres.2019.115246_bib14","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1093\/nar\/gkv107","article-title":"Accurate multiplexing and filtering for high-throughput amplicon-sequencing","volume":"43","author":"Esling","year":"2015","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"10.1016\/j.watres.2019.115246_bib15","doi-asserted-by":"crossref","DOI":"10.1128\/mSystems.00032-16","article-title":"16S rRNA amplicon sequencing for epidemiological surveys of bacteria in wildlife","volume":"1","author":"Galan","year":"2016","journal-title":"mSystems"},{"issue":"15","key":"10.1016\/j.watres.2019.115246_bib16","doi-asserted-by":"crossref","first-page":"20772","DOI":"10.2807\/1560-7917.ES2014.19.15.20772","article-title":"Comparison of diagnostic clinical samples and environmental sampling for enterovirus and parechovirus surveillance in Scotland, 2010 to 2012","volume":"19","author":"Harvala","year":"2014","journal-title":"Euro Surveill."},{"issue":"18","key":"10.1016\/j.watres.2019.115246_bib17","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00630-17","article-title":"Phylogeography of coxsackievirus A16 reveals global transmission pathways and recent emergence and spread of a recombinant genogroup","volume":"91","author":"Hassel","year":"2017","journal-title":"J.\u00a0Virol."},{"issue":"34","key":"10.1016\/j.watres.2019.115246_bib18","doi-asserted-by":"crossref","first-page":"30005","DOI":"10.2807\/1560-7917.ES.2015.20.34.30005","article-title":"Transmission patterns of human enterovirus 71 to, from and among European countries, 2003 to 2013","volume":"20","author":"Hassel","year":"2015","journal-title":"Euro Surveill."},{"issue":"22","key":"10.1016\/j.watres.2019.115246_bib19","doi-asserted-by":"crossref","first-page":"12249","DOI":"10.1128\/JVI.02075-13","article-title":"Phylogenetic patterns of human coxsackievirus B5 arise from population dynamics between two genogroups and reveal evolutionary factors of molecular adaptation and transmission","volume":"87","author":"Henquell","year":"2013","journal-title":"J.\u00a0Virol."},{"issue":"5","key":"10.1016\/j.watres.2019.115246_bib20","doi-asserted-by":"crossref","first-page":"e64","DOI":"10.1016\/S1473-3099(15)00543-5","article-title":"Global emergence of enterovirus D68: a systematic review","volume":"16","author":"Holm-Hansen","year":"2016","journal-title":"Lancet Infect. Dis."},{"issue":"36","key":"10.1016\/j.watres.2019.115246_bib21","doi-asserted-by":"crossref","first-page":"4769","DOI":"10.1016\/j.vaccine.2017.04.036","article-title":"Responding to a cVDPV1 outbreak in Ukraine: implications, challenges and opportunities","volume":"35","author":"Khetsuriani","year":"2017","journal-title":"Vaccine"},{"issue":"44","key":"10.1016\/j.watres.2019.115246_bib22","doi-asserted-by":"crossref","first-page":"20952","DOI":"10.2807\/1560-7917.ES2014.19.44.20952","article-title":"Acute flaccid paralysis following enterovirus D68 associated pneumonia, France, 2014","volume":"19","author":"Lang","year":"2014","journal-title":"Euro Surveill."},{"issue":"7","key":"10.1016\/j.watres.2019.115246_bib23","doi-asserted-by":"crossref","first-page":"20708","DOI":"10.2807\/1560-7917.ES2014.19.7.20708","article-title":"Intensified environmental surveillance supporting the response to wild poliovirus type 1 silent circulation in Israel, 2013","volume":"19","author":"Manor","year":"2014","journal-title":"Euro Surveill."},{"issue":"12","key":"10.1016\/j.watres.2019.115246_bib24","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1002\/jmv.20747","article-title":"Prospective identification of HEV-B enteroviruses during the 2005 outbreak","volume":"78","author":"Mirand","year":"2006","journal-title":"J.\u00a0Med. Virol."},{"issue":"1","key":"10.1016\/j.watres.2019.115246_bib25","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1128\/JCM.01020-07","article-title":"Prospective identification of enteroviruses involved in meningitis in 2006 through direct genotyping in cerebrospinal fluid","volume":"46","author":"Mirand","year":"2008","journal-title":"J.\u00a0Clin. Microbiol."},{"issue":"11","key":"10.1016\/j.watres.2019.115246_bib26","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.3201\/eid2211.160590","article-title":"Ambulatory pediatric surveillance of hand, foot and mouth disease as signal of an outbreak of coxsackievirus A6 infections, France, 2014-2015","volume":"22","author":"Mirand","year":"2016","journal-title":"Emerg. Infect. Dis."},{"issue":"Pt 9","key":"10.1016\/j.watres.2019.115246_bib27","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1099\/vir.0.021741-0","article-title":"Phylogenetic evidence for a recent spread of two populations of human enterovirus 71 in European countries","volume":"91","author":"Mirand","year":"2010","journal-title":"J.\u00a0Gen. Virol."},{"issue":"8","key":"10.1016\/j.watres.2019.115246_bib28","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1128\/JCM.00542-06","article-title":"Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens","volume":"44","author":"Nix","year":"2006","journal-title":"J.\u00a0Clin. Microbiol."},{"issue":"12","key":"10.1016\/j.watres.2019.115246_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145674","article-title":"Enterovirus migration patterns between France and Tunisia","volume":"10","author":"Othman","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.watres.2019.115246_bib30","unstructured":"Polio Eradication & Endgame Strategic Plan 2013-2018, 2013"},{"issue":"3","key":"10.1016\/j.watres.2019.115246_bib31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009490","article-title":"FastTree 2--approximately maximum-likelihood trees for large alignments","volume":"5","author":"Price","year":"2010","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.watres.2019.115246_bib32","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1111\/1755-0998.12402","article-title":"Tag jumps illuminated--reducing sequence-to-sample misidentifications in metabarcoding studies","volume":"15","author":"Schnell","year":"2015","journal-title":"Mol. Ecol. Resour."},{"issue":"19","key":"10.1016\/j.watres.2019.115246_bib33","doi-asserted-by":"crossref","first-page":"30226","DOI":"10.2807\/1560-7917.ES.2016.21.19.30226","article-title":"Epidemiological and clinical characteristics of patients infected with enterovirus D68, France, July to December 2014","volume":"21","author":"Schuffenecker","year":"2016","journal-title":"Euro Surveill."},{"issue":"3","key":"10.1016\/j.watres.2019.115246_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0058950","article-title":"Phylogenetic affiliation of SSU rRNA genes generated by massively parallel sequencing: new insights into the freshwater protist diversity","volume":"8","author":"Taib","year":"2013","journal-title":"PLoS One"},{"issue":"10","key":"10.1016\/j.watres.2019.115246_bib35","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol."},{"issue":"3","key":"10.1016\/j.watres.2019.115246_bib36","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1093\/inthealth\/ihx016","article-title":"The critical role of acute flaccid paralysis surveillance in the Global Polio Eradication Initiative","volume":"9","author":"Tangermann","year":"2017","journal-title":"Int. Health"},{"issue":"9","key":"10.1016\/j.watres.2019.115246_bib37","doi-asserted-by":"crossref","first-page":"2306","DOI":"10.1128\/JCM.00207-16","article-title":"Newly identified enterovirus C genotypes, identified in The Netherlands through routine sequencing of all enteroviruses detected in clinical materials from 2008 to 2015","volume":"54","author":"Van Leer-Buter","year":"2016","journal-title":"J.\u00a0Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.watres.2019.115246_bib38","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jviromet.2012.06.019","article-title":"Quantitative real-time RT-PCR assay for research studies on enterovirus infections in the central nervous system","volume":"185","author":"Volle","year":"2012","journal-title":"J.\u00a0Virol. Methods"},{"issue":"4","key":"10.1016\/j.watres.2019.115246_bib39","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/S1473-3099(13)70342-6","article-title":"Hand, foot, and mouth disease in China, 2008-12: an epidemiological study","volume":"14","author":"Xing","year":"2014","journal-title":"Lancet Infect. Dis."}],"container-title":["Water Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0043135419310206?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0043135419310206?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T02:37:29Z","timestamp":1758595049000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0043135419310206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":39,"alternative-id":["S0043135419310206"],"URL":"https:\/\/doi.org\/10.1016\/j.watres.2019.115246","relation":{},"ISSN":["0043-1354"],"issn-type":[{"value":"0043-1354","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Monitoring of enterovirus diversity in wastewater by ultra-deep sequencing: An effective complementary tool for clinical enterovirus surveillance","name":"articletitle","label":"Article Title"},{"value":"Water Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.watres.2019.115246","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"115246"}}