{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T20:37:26Z","timestamp":1793738246965,"version":"4.6.1"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015846","name":"Welsh Government","doi-asserted-by":"publisher","award":["C035\/2021\/2022"],"award-info":[{"award-number":["C035\/2021\/2022"]}],"id":[{"id":"10.13039\/100015846","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Water Research"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.watres.2024.121612","type":"journal-article","created":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T16:40:26Z","timestamp":1713026426000},"page":"121612","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Simultaneous detection and characterization of common respiratory pathogens in wastewater through genomic sequencing"],"prefix":"10.1016","volume":"256","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7158-4117","authenticated-orcid":false,"given":"Rachel C.","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kata","family":"Farkas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1516-3746","authenticated-orcid":false,"given":"Alvaro","family":"Garcia-Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Latifah","family":"Adwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7367-4338","authenticated-orcid":false,"given":"Jessica L.","family":"Kevill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth","family":"Cross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Weightman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davey L.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.watres.2024.121612_bib0001","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol"},{"key":"10.1016\/j.watres.2024.121612_bib0002","doi-asserted-by":"crossref","first-page":"848","DOI":"10.3201\/eid1205.051424","article-title":"Human bocavirus infection","volume":"12","author":"Bastien","year":"2006","journal-title":"Canada. Emerg Infect Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0003","doi-asserted-by":"crossref","first-page":"e00099","DOI":"10.1128\/mSphere.00099-17","article-title":"Retrospective surveillance of wastewater to examine seasonal dynamics of enterovirus infections","volume":"2","author":"Brinkman","year":"2017","journal-title":"mSphere"},{"key":"10.1016\/j.watres.2024.121612_bib0004","doi-asserted-by":"crossref","first-page":"7003","DOI":"10.1038\/s41467-022-33713-y","article-title":"Global disparities in SARS-CoV-2 genomic surveillance","volume":"13","author":"Brito","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.watres.2024.121612_bib0005","article-title":"Microbiome analysis for wastewater surveillance during COVID-19","volume":"13","author":"Brumfield","year":"2022","journal-title":"mBiomBio"},{"key":"10.1016\/j.watres.2024.121612_bib0006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.watres.2022.119306","article-title":"City-wide wastewater genomic surveillance through the successive emergence of SARS-CoV-2 Alpha and Delta variants","volume":"226","author":"Brunner","year":"2022","journal-title":"Water. Res."},{"key":"10.1016\/j.watres.2024.121612_bib0007","doi-asserted-by":"crossref","DOI":"10.1186\/s12889-018-5931-6","article-title":"Pathogen seasonality and links with weather in England and wales: a big data time series analysis David Stieb, Cecile Boot, Michelle Turner, Osmar Zaiane","volume":"18","author":"Cherrie","year":"2018","journal-title":"BMC Public Health"},{"key":"10.1016\/j.watres.2024.121612_bib0008","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jhin.2022.09.022","article-title":"Factors affecting turnaround time of SARS-CoV-2 sequencing for inpatient infection prevention and control decision making: analysis of data from the COG-UK HOCI study","volume":"131","author":"Colton","year":"2023","journal-title":"J. Hospital Infect."},{"key":"10.1016\/j.watres.2024.121612_bib0009","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1038\/s41591-022-01940-x","article-title":"Wastewater surveillance of pathogens can inform public health responses","volume":"28","author":"Diamond","year":"2022","journal-title":"Nat. Med."},{"key":"10.1016\/j.watres.2024.121612_bib0010","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1002\/jmv.25781","article-title":"The clinical characteristics of pneumonia patients coinfected with 2019 novel coronavirus and influenza virus in Wuhan","volume":"92","author":"Ding","year":"2020","journal-title":"China. J Med Virol"},{"key":"10.1016\/j.watres.2024.121612_bib0011","doi-asserted-by":"crossref","first-page":"369","DOI":"10.3201\/eid0703.017301","article-title":"Seasonal variation in host susceptibility and cycles of certain infectious diseases","volume":"7","author":"Dowell","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0012","doi-asserted-by":"crossref","DOI":"10.1016\/j.coesh.2020.06.001","article-title":"Wastewater and public health: the potential of wastewater surveillance for monitoring COVID-19","author":"Farkas","year":"2020","journal-title":"Curr. Opin. Environ. Sci. Health"},{"key":"10.1016\/j.watres.2024.121612_bib0013","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/mps4010017","article-title":"Concentration and quantification of sars-cov-2 RNA in wastewater using polyethylene glycol-based concentration and qRT-PCR","volume":"4","author":"Farkas","year":"2021","journal-title":"Methods Protoc."},{"key":"10.1016\/j.watres.2024.121612_bib0014","doi-asserted-by":"crossref","first-page":"e01102","DOI":"10.1128\/spectrum.01102-22","article-title":"Comparative assessment of filtration- and precipitation-based methods for the concentration of SARS-CoV-2 and other viruses from wastewater","volume":"10","author":"Farkas","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.watres.2024.121612_bib0015","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/j.1469-0691.2012.03968.x","article-title":"Seasonality of viral infections: mechanisms and unknowns","volume":"18","author":"Fisman","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.watres.2024.121612_bib0016","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.3201\/eid1708.060213.","article-title":"Human bocavirus in French children","volume":"12","author":"Foulongne","year":"2006","journal-title":"Emerging Infect. Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0017","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/S0140-6736(20)30925-9","article-title":"Global burden of 369 diseases and injuries in 204 countries and territories, 1990\u20132019: a systematic analysis for the Global Burden of Disease Study 2019","volume":"396","year":"2020","journal-title":"The Lancet"},{"key":"10.1016\/j.watres.2024.121612_bib0018","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1128\/JCM.03060-15","article-title":"Unbiased detection of respiratory viruses by use of RNA sequencing-based metagenomics: a systematic comparison to a commercial PCR panel","volume":"54","author":"Graf","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.watres.2024.121612_bib0019","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1038\/s41579-021-00573-0","article-title":"SARS-CoV-2 variants, spike mutations and immune escape","volume":"19","author":"Harvey","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.watres.2024.121612_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2021.117214","article-title":"Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK","volume":"200","author":"Hillary","year":"2021","journal-title":"Water. Res."},{"key":"10.1016\/j.watres.2024.121612_bib0021","doi-asserted-by":"crossref","DOI":"10.1016\/j.eclinm.2021.100986","article-title":"Global burden of upper respiratory infections in 204 countries and territories, from 1990 to 2019","volume":"37","author":"Jin","year":"2021","journal-title":"EClinicalMedicine"},{"key":"10.1016\/j.watres.2024.121612_bib0022","article-title":"Evaluation of sequence hybridization for respiratory viruses using the Twist Bioscience Respiratory Virus Research panel and the OneCodex Respiratory Virus sequence analysis workflow","volume":"9","author":"Kapel","year":"2023","journal-title":"Microb. Genom."},{"key":"10.1016\/j.watres.2024.121612_bib0023","doi-asserted-by":"crossref","first-page":"5561","DOI":"10.1111\/1462-2920.16175","article-title":"Development of a machine-learning model to identify the impacts of pesticides characteristics on soil microbial communities from high-throughput sequencing data","volume":"24","author":"Ke","year":"2022","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.watres.2024.121612_bib0024","doi-asserted-by":"crossref","DOI":"10.1016\/S2214-109X(23)00170-5","article-title":"Wastewater monitoring can anchor global disease surveillance systems","author":"Keshaviah","year":"2023","journal-title":"Lancet Glob. Health"},{"key":"10.1016\/j.watres.2024.121612_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.151916","article-title":"A comparison of precipitation and filtration-based SARS-CoV-2 recovery methods and the influence of temperature, turbidity, and surfactant load in urban wastewater","volume":"808","author":"Kevill","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.watres.2024.121612_bib0026","doi-asserted-by":"crossref","DOI":"10.1016\/j.onehlt.2023.100536","article-title":"Significance of wastewater surveillance in detecting the prevalence of SARS-CoV-2 variants and other respiratory viruses in the community \u2013 a multi-site evaluation","volume":"16","author":"Khan","year":"2023","journal-title":"One Health"},{"key":"10.1016\/j.watres.2024.121612_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2023.105381","article-title":"Disruption of seasonal enterovirus and parechovirus detections in the CSF and plasma of children during the COVID-19 pandemic","volume":"160","author":"Lee","year":"2023","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.watres.2024.121612_bib0028","article-title":"Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities","volume":"12","author":"Li","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.watres.2024.121612_bib0029","doi-asserted-by":"crossref","first-page":"e1031","DOI":"10.1016\/S2214-109X(19)30264-5","article-title":"Global patterns in monthly activity of influenza virus, respiratory syncytial virus, parainfluenza virus, and metapneumovirus: a systematic analysis","volume":"7","author":"Li","year":"2019","journal-title":"Lancet Glob. Health"},{"key":"10.1016\/j.watres.2024.121612_bib0030","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-016-1829-z","article-title":"Epidemiological aspects of rotavirus and adenovirus in hospitalized children with diarrhea: a 5-year survey in Beijing","volume":"16","author":"Liu","year":"2016","journal-title":"BMC. Infect. Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0031","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.1128\/JVI.01680-06","article-title":"Influenza seasonality: underlying causes and modeling theories","volume":"81","author":"Lofgren","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.watres.2024.121612_bib0032","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.jcv.2007.01.008","article-title":"Human bocavirus in Italian patients with respiratory diseases","volume":"38","author":"Maggi","year":"2007","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.watres.2024.121612_bib0033","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.653399","article-title":"Respiratory Co-infections: modulators of SARS-CoV-2 Patients\u2019 clinical Sub-phenotype","volume":"12","author":"Mehta","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.watres.2024.121612_bib0034","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.150264","article-title":"A wastewater-based epidemiology tool for COVID-19 surveillance in portugal","volume":"804","author":"Monteiro","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.watres.2024.121612_bib0035","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1146\/annurev-virology-012420-022445","article-title":"Annual review of virology seasonality of respiratory viral infections","volume":"29","author":"Moriyama","year":"2020","journal-title":"Annu Rev. Virol."},{"key":"10.1016\/j.watres.2024.121612_bib0036","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-021-06785-2","article-title":"Coronavirus seasonality, respiratory infections and weather","volume":"21","author":"Nichols","year":"2021","journal-title":"BMC. Infect. Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0037","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1093\/infdis\/jiy056","article-title":"Respiratory syncytial virus seasonality: a global overview","volume":"217","author":"Obando-Pacheco","year":"2018","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0038","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.28753","article-title":"Metagenomics characterization of respiratory viral RNA pathogens in children under five years with severe acute respiratory infection in the Free State, South Africa","volume":"95","author":"Ogunbayo","year":"2023","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.watres.2024.121612_bib0039","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1111\/ped.12147","article-title":"WU polyomavirus detected in respiratory tract specimens from young children in Japan","volume":"55","author":"Okada","year":"2013","journal-title":"Pediatric. Int."},{"key":"10.1016\/j.watres.2024.121612_bib0040","doi-asserted-by":"crossref","first-page":"44","DOI":"10.5864\/d2021-015","article-title":"Wastewater-based epidemiology: current uses and future opportunities as a public health surveillance tool","volume":"64","author":"O'Keeffe","year":"2021","journal-title":"Environ. Health Rev."},{"key":"10.1016\/j.watres.2024.121612_bib0041","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1038\/s41587-020-0684-z","article-title":"Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics","volume":"38","author":"Peccia","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.watres.2024.121612_bib0042","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1097\/QCO.0000000000000187","article-title":"The epidemiology of non-polio enteroviruses: recent advances and outstanding questions","volume":"28","author":"Pons-Salort","year":"2015","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.watres.2024.121612_bib0043","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2021.116810","article-title":"Wastewater-based epidemiology as a useful tool to track SARS-CoV-2 and support public health policies at municipal level in Brazil","volume":"191","author":"Prado","year":"2021","journal-title":"Water. Res."},{"key":"10.1016\/j.watres.2024.121612_bib0044","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S0140-6736(21)00150-1","article-title":"An action plan for pan-European defence against new SARS-CoV-2 variants","volume":"397","author":"Priesemann","year":"2021","journal-title":"The Lancet"},{"key":"10.1016\/j.watres.2024.121612_bib0045","series-title":"R: A Language and Environment for Statistical Computing","year":"2022"},{"key":"10.1016\/j.watres.2024.121612_bib0046","doi-asserted-by":"crossref","unstructured":"Rieckermann, J., Neumann, M., Ort, C., Huisman, J.L., Gujer, W., 2005. Dispersion coefficients of sewers from tracer experiments.","DOI":"10.2166\/wst.2005.0124"},{"key":"10.1016\/j.watres.2024.121612_bib0047","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-022-30518-x","article-title":"Co-infection with SARS-CoV-2 omicron and delta variants revealed by genomic surveillance","volume":"13","author":"Rockett","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.watres.2024.121612_bib0048","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.149794","article-title":"Enumerating asymptomatic COVID-19 cases and estimating SARS-CoV-2 fecal shedding rates via wastewater-based epidemiology","volume":"801","author":"Schmitz","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.watres.2024.121612_bib0049","doi-asserted-by":"crossref","first-page":"34","DOI":"10.15199\/17.2016.4.7","article-title":"Dispersion process in urban sewer networks under dry weather conditions","volume":"1","author":"Sok\u00e1\u010d","year":"2016","journal-title":"GAZ, WODA I TECHNIKA SANITARNA"},{"key":"10.1016\/j.watres.2024.121612_bib0050","doi-asserted-by":"crossref","DOI":"10.1061\/JOEEDU.EEENG-7134","article-title":"Quantifying mixing in sewer networks for source localization","volume":"149","author":"Sonnenwald","year":"2023","journal-title":"J. Environ. Eng."},{"key":"10.1016\/j.watres.2024.121612_bib0051","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1002\/jmv.21283","article-title":"Longitudinal observation of parechovirus in stool samples from Norwegian infants","volume":"80","author":"Tapia","year":"2008","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.watres.2024.121612_bib0052","article-title":"Comprehensive wastewater sequencing reveals community and variant dynamics of the collective human Virome","author":"Tisza","year":"2023","journal-title":"medRxiv"},{"key":"10.1016\/j.watres.2024.121612_bib0053","unstructured":"UK Health Security Agency, 2023. Surveillance of influenza and other seasonal respiratory viruses in the UK, winter 2022 to 2023."},{"key":"10.1016\/j.watres.2024.121612_bib0054","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.150121","article-title":"SARS-CoV-2 RNA concentrations in wastewater foreshadow dynamics and clinical presentation of new COVID-19 cases","volume":"805","author":"Wu","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.watres.2024.121612_bib0055","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","article-title":"A new coronavirus associated with human respiratory disease in China","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"}],"container-title":["Water Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004313542400513X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004313542400513X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T13:18:53Z","timestamp":1717766333000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S004313542400513X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":55,"alternative-id":["S004313542400513X"],"URL":"https:\/\/doi.org\/10.1016\/j.watres.2024.121612","relation":{},"ISSN":["0043-1354"],"issn-type":[{"value":"0043-1354","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simultaneous detection and characterization of common respiratory pathogens in wastewater through genomic sequencing","name":"articletitle","label":"Article Title"},{"value":"Water Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.watres.2024.121612","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"121612"}}