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Minimum inhibitory concentration (MIC) or MEC (minimum effective concentration) for 6 antifungals were determined by antifungal susceptibility testing <jats:italic>in\u00a0vitro<\/jats:italic>. With Bayes rule, sensitivity and specificity of both MALDI\u2010TOF MS and culture test methods were computed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cultures yielded 42 fungal isolates which were confirmed as species (specified against each species) belonging to 8 genera, <jats:italic>A. niger<\/jats:italic> 22, <jats:italic>Candida<\/jats:italic> sp. 7, <jats:italic>A. fumigatus<\/jats:italic> 3, <jats:italic>A. flavus<\/jats:italic> 3, mixed <jats:italic>Aspergillus<\/jats:italic> sp. 3, <jats:italic>Mucor<\/jats:italic> sp. 2, <jats:italic>Rhizopus<\/jats:italic> sp. 1, and <jats:italic>Scopulariopsis<\/jats:italic> sp.1; and MALDI\u2010TOF\u2010MS system also confirmed those isolates. <jats:italic>In\u00a0vitro<\/jats:italic> antifungal susceptibility testing with terms of MIC 50 and MIC 90, isolates fungi were highly susceptible to 6 antifungals; and caspofungin was the most active antifungal. The high value of specificity 84.6%, suggested a limited loss of confidence on the culture test at the absence of an infection, in comparison to MALDI\u2010TOF\u2010MS. Sensitivity of the culture test was 87.5%; this high figure strongly approves the culture test to be the dependable method for the otomycosis diagnosis, when the infection is stable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Based on sensitivity and specificity together the culture test could be done in face of the gold\u2010standard MALDI\u2010TOF\u2010MS system the estimating both methods.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.wjorl.2018.03.006","type":"journal-article","created":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T14:01:50Z","timestamp":1546610510000},"page":"6-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bayesian analysis of two methods MALDI\u2010TOF\u2010MS system and culture test in\u00a0otomycosis infection"],"prefix":"10.1002","volume":"5","author":[{"given":"Sibanarayan","family":"Rath","sequence":"first","affiliation":[{"name":"Central Research Laboratory Institute of Medical Sciences and Sum Hospital Siksha \u2018O\u2019 Anusandhan University Odisha India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saumya Ranjan","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Ear Nose and Throat Institute of Medical Sciences and Sum Hospital Siksha \u2018O\u2019 Anusandhan University Odisha India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabindra Nath","family":"Padhy","sequence":"additional","affiliation":[{"name":"Central Research Laboratory Institute of Medical Sciences and Sum Hospital Siksha \u2018O\u2019 Anusandhan University Odisha India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"927","article-title":"Otitis externa: a practical guide to treatment and prevention","volume":"63","author":"Sander R.","year":"2001","journal-title":"Am Fam Physician"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2006.07.008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.2003.00902.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0933-7407.2001.00682.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2005.01.022"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1258\/0022215011909486"},{"key":"e_1_2_8_8_1","first-page":"23","article-title":"1% bifonazole lotion in the therapy of otomycosis","volume":"130","author":"Piantoni S.","year":"1989","journal-title":"Clin Ter"},{"key":"e_1_2_8_9_1","first-page":"82","article-title":"Fatal cutaneous mucormycosis after kidney transplant","volume":"13","author":"Davuodi S.","year":"2015","journal-title":"Exp Clin Transpl"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2013.1.423"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00298.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-012-0094-x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac010651t"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200468"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myu064"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01437-13"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2015.06.065"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjorl.2016.03.008"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00737-12"},{"volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi","year":"2008","author":"Clinical and Laboratory Standards Institute","key":"e_1_2_8_20_1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006692-0"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2953-2958.2002"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02071-09"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12000"}],"container-title":["World Journal of Otorhinolaryngology - 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