{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T11:53:48Z","timestamp":1772711628310,"version":"3.50.1"},"reference-count":38,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32-AI-055400"],"award-info":[{"award-number":["T32-AI-055400"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019145","name":"Boston Biomedical","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019145","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004325","name":"AstraZeneca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004325","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011094","name":"Public Health Agency of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011094","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016255","name":"INOVIO Pharmaceuticals","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016255","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016302","name":"Coalition for Epidemic Preparedness Innovations","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016302","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["T32 CA09171"],"award-info":[{"award-number":["T32 CA09171"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017119","name":"Wistar Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017119","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports Medicine"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.xcrm.2022.100693","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T02:28:20Z","timestamp":1656383300000},"page":"100693","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Mucosal chemokine adjuvant enhances synDNA vaccine-mediated responses to SARS-CoV-2 and provides heterologous protection in\u00a0vivo"],"prefix":"10.1016","volume":"3","author":[{"given":"Ebony N.","family":"Gary","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Tursi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryce","family":"Warner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth M.","family":"Parzych","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali R.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew","family":"Frase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estella","family":"Moffat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carissa","family":"Embury-Hyatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor R.F.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate E.","family":"Broderick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Humeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darwyn","family":"Kobasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ami","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel W.","family":"Kulp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B.","family":"Weiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xcrm.2022.100693_bib1","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.3390\/vaccines9101145","article-title":"Rapidly declining SARS-CoV-2 antibody titers within 4\u00a0months after BNT162b2 vaccination","volume":"9","author":"Jo","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.xcrm.2022.100693_bib2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1056\/NEJMc2032195","article-title":"Durability of responses after SARS-CoV-2 mRNA-1273 vaccination","volume":"384","author":"Widge","year":"2021","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.xcrm.2022.100693_bib3","series-title":"Declined antibody responses to COVID-19 mRNA vaccine within first three months","author":"Naaber","year":"2021"},{"key":"10.1016\/j.xcrm.2022.100693_bib4","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1038\/s41467-021-21111-9","article-title":"Rapid decline of neutralizing antibodies against SARS-CoV-2 among infected healthcare workers","volume":"12","author":"Marot","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.xcrm.2022.100693_bib5","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1001\/jama.2021.11656","article-title":"Age-dependent neutralization of SARS-CoV-2 and P. 1 variant by vaccine immune serum samples","volume":"326","author":"Bates","year":"2021","journal-title":"JAMA"},{"key":"10.1016\/j.xcrm.2022.100693_bib6","doi-asserted-by":"crossref","first-page":"e2124331","DOI":"10.1001\/jamanetworkopen.2021.24331","article-title":"Comparison of SARS-CoV-2 antibody response by age among recipients of the BNT162b2 vs the mRNA-1273 vaccine","volume":"4","author":"Richards","year":"2021","journal-title":"JAMA Netw. Open"},{"key":"10.1016\/j.xcrm.2022.100693_bib7","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1056\/NEJMoa2035389","article-title":"Efficacy and safety of the mRNA-1273 SARS-CoV-2 vaccine","volume":"384","author":"Baden","year":"2021","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.xcrm.2022.100693_bib8","first-page":"1","article-title":"High level of protection against COVID-19 after two doses of BNT162b2 vaccine in the working age population\u2013first results from a cohort study in Southern Sweden","author":"Bj\u00f6rk","year":"2021","journal-title":"Infect. Dis. (Lond.)"},{"key":"10.1016\/j.xcrm.2022.100693_bib9","first-page":"2100640","article-title":"Vaccine effectiveness against SARS-CoV-2 transmission and infections among household and other close contacts of confirmed cases, The Netherlands, February to May 2021","volume":"26","author":"de Gier","year":"2021","journal-title":"Euro Surveill."},{"key":"10.1016\/j.xcrm.2022.100693_bib10","first-page":"2100977","article-title":"Vaccine effectiveness against SARS-CoV-2 transmission to household contacts during dominance of Delta variant (B.1.617.2), The Netherlands, August to September 2021","volume":"26","author":"de Gier","year":"2021","journal-title":"Euro Surveill."},{"key":"10.1016\/j.xcrm.2022.100693_bib11","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/imr.12087","article-title":"Tissue-resident memory T\u00a0cells","volume":"255","author":"Shin","year":"2013","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.xcrm.2022.100693_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2018\/3734207","article-title":"Defensive driving: directing HIV-1 vaccine-induced humoral immunity to the mucosa with chemokine adjuvants","volume":"2018","author":"Gary","year":"2018","journal-title":"J.\u00a0Immunol. Res."},{"key":"10.1016\/j.xcrm.2022.100693_bib13","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/s41541-019-0129-1","article-title":"Successful application of prime and pull strategy for a therapeutic HSV vaccine","volume":"4","author":"Bernstein","year":"2019","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.xcrm.2022.100693_bib14","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/nature11522","article-title":"A vaccine strategy that protects against genital herpes by establishing local memory T\u00a0cells","volume":"491","author":"Shin","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.xcrm.2022.100693_bib15","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1172\/JCI17244","article-title":"CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells","volume":"111","author":"Kunkel","year":"2003","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.xcrm.2022.100693_bib16","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s13238-012-2927-3","article-title":"CCR10 and its ligands in regulation of epithelial immunity and diseases","volume":"3","author":"Xiong","year":"2012","journal-title":"Protein Cell"},{"key":"10.1016\/j.xcrm.2022.100693_bib17","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/nm0202-157","article-title":"CCL27\u2013CCR10 interactions regulate T cell\u2013mediated skin inflammation","volume":"8","author":"Homey","year":"2002","journal-title":"Nat. Med."},{"key":"10.1016\/j.xcrm.2022.100693_bib18","doi-asserted-by":"crossref","first-page":"3465","DOI":"10.4049\/jimmunol.164.7.3465","article-title":"Cutting edge: the orphan chemokine receptor G protein-coupled receptor-2 (GPR-2, CCR10) binds the skin-associated chemokine CCL27 (CTACK\/ALP\/ILC)","volume":"164","author":"Homey","year":"2000","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.xcrm.2022.100693_bib19","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1086\/592448","article-title":"Severe tuberculosis induces unbalanced up-regulation of gene networks and overexpression ofIL-22, MIP-1\u03b1, CCL27, IP-10, CCR4, CCR5, CXCR3, PD1, PDL2, IL-3, IFN-\u03b2, TIM1, andTLR2but low antigen-specific cellular responses","volume":"198","author":"Qiu","year":"2008","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.xcrm.2022.100693_bib20","doi-asserted-by":"crossref","first-page":"6309","DOI":"10.4049\/jimmunol.181.9.6309","article-title":"An indispensable role for the chemokine receptor CCR10 in IgA antibody-secreting cell accumulation","volume":"181","author":"Morteau","year":"2008","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.xcrm.2022.100693_bib21","doi-asserted-by":"crossref","first-page":"E1035","DOI":"10.1073\/pnas.1100156108","article-title":"Critical roles of chemokine receptor CCR10 in regulating memory IgA responses in intestines","volume":"108","author":"Hu","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xcrm.2022.100693_bib22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/mi.2015.31","article-title":"Chemokine-adjuvanted electroporated DNA vaccine induces substantial protection from simian immunodeficiency virus vaginal challenge","volume":"9","author":"Kutzler","year":"2016","journal-title":"Mucosal Immunol."},{"key":"10.1016\/j.xcrm.2022.100693_bib23","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.1016\/j.vaccine.2020.01.023","article-title":"CCR10 expression is required for the adjuvant activity of the mucosal chemokine CCL28 when delivered in the context of an HIV-1 Env DNA vaccine","volume":"38","author":"Gary","year":"2020","journal-title":"Vaccine"},{"key":"10.1016\/j.xcrm.2022.100693_bib24","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1016\/j.vaccine.2009.10.095","article-title":"Systemic immunization with CCL27\/CTACK modulates immune responses at mucosal sites in mice and macaques","volume":"28","author":"Kraynyak","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.xcrm.2022.100693_bib25","doi-asserted-by":"crossref","first-page":"102699","DOI":"10.1016\/j.isci.2021.102699","article-title":"A novel mouse AAV6 hACE2 transduction model of wild-type SARS-CoV-2 infection studied using synDNA immunogens","volume":"24","author":"Gary","year":"2021","journal-title":"iScience"},{"key":"10.1016\/j.xcrm.2022.100693_bib26","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1084\/jem.192.5.761","article-title":"Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity","volume":"192","author":"Kunkel","year":"2000","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.xcrm.2022.100693_bib27","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.immuni.2004.08.011","article-title":"Retinoic acid imprints gut-homing specificity on T\u00a0cells","volume":"21","author":"Iwata","year":"2004","journal-title":"Immunity"},{"key":"10.1016\/j.xcrm.2022.100693_bib28","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/S1367-5931(03)00086-3","article-title":"Therapeutic applications in the chemokine superfamily","volume":"7","author":"Houshmand","year":"2003","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.xcrm.2022.100693_bib29","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.4161\/hv.22574","article-title":"Generation of antigen-specific immunity following systemic immunization with DNA vaccine encoding CCL25 chemokine immunoadjuvant","volume":"8","author":"Kathuria","year":"2012","journal-title":"Hum. Vaccines Immunother."},{"key":"10.1016\/j.xcrm.2022.100693_bib30","doi-asserted-by":"crossref","first-page":"11173","DOI":"10.1128\/JVI.74.23.11173-11180.2000","article-title":"DNA vaccines encoding interleukin-8 and RANTES enhance antigen-specific Th1-type CD4+ T-cell-mediated protective immunity against herpes simplex virus type 2 in\u00a0vivo","volume":"74","author":"Sin","year":"2000","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.xcrm.2022.100693_bib31","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/gt.2009.112","article-title":"Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in\u00a0vivo","volume":"17","author":"Kutzler","year":"2010","journal-title":"Gene Ther."},{"key":"10.1016\/j.xcrm.2022.100693_bib32","doi-asserted-by":"crossref","first-page":"903","DOI":"10.4049\/jimmunol.1901184","article-title":"Chemokine-adjuvanted plasmid DNA induces homing of antigen-specific and non\u2013antigen-specific B and T\u00a0cells to the intestinal and genital mucosae","volume":"204","author":"Aldon","year":"2020","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.xcrm.2022.100693_bib33","volume":"92","author":"Khan","year":"2018","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.xcrm.2022.100693_bib34","doi-asserted-by":"crossref","first-page":"137079","DOI":"10.1172\/jci.insight.137079","article-title":"Robust antibody and cellular responses induced by DNA-only vaccination for HIV","volume":"5","author":"De Rosa","year":"2020","journal-title":"JCI Insight"},{"key":"10.1016\/j.xcrm.2022.100693_bib35","doi-asserted-by":"crossref","first-page":"e003019","DOI":"10.1136\/jitc-2021-003019","article-title":"Phase 1 study of safety, tolerability and immunogenicity of the human telomerase (hTERT)-encoded DNA plasmids INO-1400 and INO-1401 with or without IL-12 DNA plasmid INO-9012 in adult patients with solid tumors","volume":"9","author":"Vonderheide","year":"2021","journal-title":"J.\u00a0Immunother. Cancer"},{"key":"10.1016\/j.xcrm.2022.100693_bib36","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1007\/s00262-018-2236-7","article-title":"A phase I\/randomized phase II study of GM. CD40L vaccine in combination with CCL21 in patients with advanced lung adenocarcinoma","volume":"67","author":"Gray","year":"2018","journal-title":"Cancer Immunol. Immunother."},{"key":"10.1016\/j.xcrm.2022.100693_bib37","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/bs.mie.2015.09.031","article-title":"Production of recombinant chemokines and validation of refolding","volume":"570","author":"Veldkamp","year":"2016","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.xcrm.2022.100693_bib38","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1126\/science.abc1669","article-title":"SARS-CoV-2 productively infects human gut enterocytes","volume":"369","author":"Lamers","year":"2020","journal-title":"Science"}],"container-title":["Cell Reports Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666379122002294?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666379122002294?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T22:50:39Z","timestamp":1762987839000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666379122002294"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":38,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["S2666379122002294"],"URL":"https:\/\/doi.org\/10.1016\/j.xcrm.2022.100693","relation":{},"ISSN":["2666-3791"],"issn-type":[{"value":"2666-3791","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mucosal chemokine adjuvant enhances synDNA vaccine-mediated responses to SARS-CoV-2 and provides heterologous protection in\u00a0vivo","name":"articletitle","label":"Article Title"},{"value":"Cell Reports Medicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xcrm.2022.100693","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"100693"}}