{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T10:32:59Z","timestamp":1784975579664,"version":"3.55.0"},"reference-count":27,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["fertstertreports.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["F&amp;S Reports"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1016\/j.xfre.2021.06.004","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:30:04Z","timestamp":1624926604000},"page":"269-274","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Is there still a role for a cleavage-stage embryo transfer?"],"prefix":"10.1016","volume":"2","author":[{"suffix":"II","given":"Michael F.","family":"Neblett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tana","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffanny L.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C.","family":"Baumgarten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles C.","family":"Coddington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4591-4207","authenticated-orcid":false,"given":"Yulian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra C.","family":"Shenoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xfre.2021.06.004_bib1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/humrep\/13.1.169","article-title":"Evolution of a culture protocol for successful blastocyst development and pregnancy","volume":"13","author":"Jones","year":"1998","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfre.2021.06.004_bib2","doi-asserted-by":"crossref","DOI":"10.1002\/14651858.CD007876.pub2","article-title":"Culture media for human pre-implantation embryos in assisted reproductive technology cycles","author":"Youssef","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"10.1016\/j.xfre.2021.06.004_bib3","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0093-691X(97)00404-4","article-title":"Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture","volume":"49","author":"Gardner","year":"1998","journal-title":"Theriogenology"},{"key":"10.1016\/j.xfre.2021.06.004_bib4","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0015-0282(16)58097-2","article-title":"Environment of the preimplantation human embryo in\u00a0vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells","volume":"65","author":"Gardner","year":"1996","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib5","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1093\/humrep\/deh536","article-title":"Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial","volume":"19","author":"Staessen","year":"2004","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfre.2021.06.004_bib6","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/332459a0","article-title":"Human gene expression first occurs between the four- and eight-cell stages of preimplantation development","volume":"332","author":"Braude","year":"1988","journal-title":"Nature"},{"key":"10.1016\/j.xfre.2021.06.004_bib7","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/(SICI)1098-2795(199712)48:4<442::AID-MRD4>3.0.CO;2-Q","article-title":"Paternal transcripts for glucose-6-phosphate dehydrogenase and adenosine deaminase are first detectable in the human preimplantation embryo at the three- to four-cell stage","volume":"48","author":"Taylor","year":"1997","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.xfre.2021.06.004_bib8","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/mrd.1080380107","article-title":"Evidence for a strong paternal effect on human preimplantation embryo development and blastocyst formation","volume":"38","author":"Janny","year":"1994","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.xfre.2021.06.004_bib9","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1093\/humrep\/16.5.918","article-title":"The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in\u00a0vitro","volume":"16","author":"Miller","year":"2001","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfre.2021.06.004_bib10","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.fertnstert.2016.06.029","article-title":"Cleavage-stage or blastocyst transfer: what are the benefits and harms?","volume":"106","author":"Glujovsky","year":"2016","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib11","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1016\/S0015-0282(01)02018-0","article-title":"Increasing levels of estradiol are deleterious to embryonic implantation because they directly affect the embryo","volume":"76","author":"Valbuena","year":"2001","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib12","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1093\/humrep\/dei291","article-title":"Preimplantation genetic screening reveals a high incidence of aneuploidy and mosaicism in embryos from young women undergoing IVF","volume":"21","author":"Baart","year":"2006","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfre.2021.06.004_bib13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S1472-6483(10)61810-X","article-title":"Chromosome mosaicism in cleavage-stage human embryos: evidence of a maternal age effect","volume":"4","author":"Munn\u00e9","year":"2002","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.xfre.2021.06.004_bib14","doi-asserted-by":"crossref","first-page":"194","DOI":"10.4103\/0974-1208.142481","article-title":"A comparative study between cleavage stage embryo transfer at day 3 and blastocyst stage transfer at day 5 in in-vitro fertilization\/intra-cytoplasmic sperm injection on clinical pregnancy rates","volume":"7","author":"Kaur","year":"2014","journal-title":"J Hum Reprod Sci"},{"key":"10.1016\/j.xfre.2021.06.004_bib15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1093\/humrep\/dem339","article-title":"Live birth rates after transfer of equal number of blastocysts or cleavage-stage embryos in IVF. A systematic review and meta-analysis","volume":"23","author":"Papanikolaou","year":"2008","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfre.2021.06.004_bib16","doi-asserted-by":"crossref","DOI":"10.1002\/14651858.CD002118.pub5","article-title":"Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology","author":"Glujovsky","year":"2016","journal-title":"Cochrane Database Syst Rev"},{"key":"10.1016\/j.xfre.2021.06.004_bib17","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1016\/j.fertnstert.2008.09.023","article-title":"Elective single embryo transfer: a 6-year progressive implementation of 784 single blastocyst transfers and the influence of payment method on patient choice","volume":"92","author":"Stillman","year":"2009","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib18","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1016\/j.fertnstert.2008.12.137","article-title":"Comparison of pregnancy outcomes in elective single blastocyst transfer versus double blastocyst transfer stratified by age","volume":"93","author":"Mullin","year":"2010","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib19","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.fertnstert.2017.07.1167","article-title":"Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology\u00a0outcomes between 2004 and 2013","volume":"108","author":"Mersereau","year":"2017","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib20","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0015-0282(02)04948-8","article-title":"Role of reactive oxygen species in the pathophysiology of human reproduction","volume":"79","author":"Agarwal","year":"2003","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib21","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.fertnstert.2006.02.088","article-title":"Oxidative stress in an assisted reproductive techniques setting","volume":"86","author":"Agarwal","year":"2006","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib22","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/S0015-0282(99)00409-4","article-title":"Introduction of blastocyst culture and transfer for all patients in an in\u00a0vitro fertilization program","volume":"72","author":"Marek","year":"1999","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib23","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.fertnstert.2019.01.033","article-title":"Fertility treatment when the prognosis is very poor or futile: an Ethics Committee opinion","volume":"111","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfre.2021.06.004_bib24","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1530\/rep.1.00241","article-title":"Deadly decisions: the role of genes regulating programmed cell death in human preimplantation embryo development","volume":"128","author":"Jurisicova","year":"2004","journal-title":"Reproduction"},{"key":"10.1016\/j.xfre.2021.06.004_bib25","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/j.rbmo.2019.08.003","article-title":"When only one embryo is available, is it better to transfer on day 3 or to grow on?","volume":"39","author":"Xiao","year":"2019","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.xfre.2021.06.004_bib26","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s10815-014-0387-9","article-title":"Ongoing and cumulative pregnancy rate after cleavage-stage versus blastocyst-stage embryo transfer using vitrification for cryopreservation: impact of age on the results","volume":"32","author":"Fern\u00e1ndez-Shaw","year":"2015","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.xfre.2021.06.004_bib27","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s10815-017-1092-2","article-title":"No advantage of fresh blastocyst versus cleavage stage embryo transfer in women under the age of 39: a randomized controlled study","volume":"35","author":"Levi-Setti","year":"2018","journal-title":"J Assist Reprod Genet"}],"container-title":["F&amp;S Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666334121000726?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666334121000726?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T21:04:45Z","timestamp":1762981485000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666334121000726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["S2666334121000726"],"URL":"https:\/\/doi.org\/10.1016\/j.xfre.2021.06.004","relation":{},"ISSN":["2666-3341"],"issn-type":[{"value":"2666-3341","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Is there still a role for a cleavage-stage embryo transfer?","name":"articletitle","label":"Article Title"},{"value":"F&S Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xfre.2021.06.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Inc. on behalf of American Society for Reproductive Medicine.","name":"copyright","label":"Copyright"}]}}