{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T04:39:31Z","timestamp":1777264771498,"version":"3.51.4"},"reference-count":47,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR001425"],"award-info":[{"award-number":["UL1TR001425"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["fertstertscience.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["F&S Science"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.xfss.2025.07.004","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T16:48:08Z","timestamp":1753202888000},"page":"463-474","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Compressive force induces differential gene and protein expression in uterine fibroids"],"prefix":"10.1016","volume":"6","author":[{"given":"Carolyn A.","family":"Nietupski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan R.","family":"Sax","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose","family":"Dean","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreja","family":"Moset Zupan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily G.","family":"Hurley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8092-5067","authenticated-orcid":false,"given":"Stacey C.","family":"Schutte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xfss.2025.07.004_bib1","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1093\/humrep\/deq222","article-title":"Decreased pregnancy rate is linked to abnormal uterine peristalsis caused by intramural fibroids","volume":"25","author":"Yoshino","year":"2010","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfss.2025.07.004_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232214201","article-title":"Biomechanical forces determine fibroid stem cell transformation and the receptivity status of the endometrium: a critical appraisal","volume":"23","author":"Celik","year":"2022","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib3","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.fertnstert.2019.02.008","article-title":"Uterine polyps, adenomyosis, leiomyomas, and endometrial receptivity","volume":"111","author":"Munro","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfss.2025.07.004_bib4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1177\/1933719116654993","article-title":"Surgery for benign gynecological disorders improve endometrium receptivity","volume":"24","author":"Celik","year":"2017","journal-title":"Reprod Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib5","doi-asserted-by":"crossref","first-page":"113","DOI":"10.31083\/j.ceog4905114","article-title":"Receptivity-based uterine fibroid surgery: an updated systematic review of the evidence","volume":"49","author":"Celik","year":"2022","journal-title":"Clin Exp Obstet Gynecol"},{"key":"10.1016\/j.xfss.2025.07.004_bib6","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1007\/s43032-021-00656-6","article-title":"Uterine fibroids and pregnancy: how do they affect each other?","volume":"29","author":"Coutinho","year":"2022","journal-title":"Reprod Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib7","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1067\/mob.2003.99","article-title":"High cumulative incidence of uterine leiomyoma in black and white women: ultrasound evidence","volume":"188","author":"Baird","year":"2003","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.xfss.2025.07.004_bib8","doi-asserted-by":"crossref","first-page":"211.e1","DOI":"10.1016\/j.ajog.2011.12.002","article-title":"The estimated annual cost of uterine leiomyomata in the United States","volume":"206","author":"Cardozo","year":"2012","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.xfss.2025.07.004_bib9","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.matbio.2011.09.001","article-title":"Characterization of tissue biomechanics and mechanical signaling in uterine leiomyoma","volume":"31","author":"Norian","year":"2012","journal-title":"Matrix Biol"},{"key":"10.1016\/j.xfss.2025.07.004_bib10","doi-asserted-by":"crossref","first-page":"474.e1","DOI":"10.1016\/j.ajog.2007.11.057","article-title":"Mechanical homeostasis is altered in uterine leiomyoma","volume":"198","author":"Rogers","year":"2008","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.xfss.2025.07.004_bib11","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/783289","article-title":"The extracellular matrix contributes to mechanotransduction in uterine fibroids","volume":"2014","author":"Leppert","year":"2014","journal-title":"Obstet Gynecol Int"},{"key":"10.1016\/j.xfss.2025.07.004_bib12","doi-asserted-by":"crossref","first-page":"0004","DOI":"10.1038\/s41551-016-0004","article-title":"Solid stress and elastic energy as measures of tumour mechanopathology","volume":"1","author":"Nia","year":"2016","journal-title":"Nat Biomed Eng"},{"key":"10.1016\/j.xfss.2025.07.004_bib13","doi-asserted-by":"crossref","first-page":"15101","DOI":"10.1073\/pnas.1213353109","article-title":"Causes, consequences, and remedies for growth-induced solid stress in murine and human tumors","volume":"109","author":"Stylianopoulos","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.xfss.2025.07.004_bib14","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1007\/s43032-023-01267-z","article-title":"Differential response to mechanical cues in uterine fibroid versus paired myometrial cells","volume":"30","author":"Schutte","year":"2023","journal-title":"Reprod Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib15","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1210\/en.2017-03191","article-title":"The AKT\/BCL-2 axis mediates survival of uterine leiomyoma in a novel 3D spheroid model","volume":"159","author":"Vidimar","year":"2018","journal-title":"Endocrinology"},{"key":"10.1016\/j.xfss.2025.07.004_bib16","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1038\/s41374-018-0117-5","article-title":"Application of ex-vivo spheroid model system for the analysis of senescence and senolytic phenotypes in uterine leiomyoma","volume":"98","author":"Xie","year":"2018","journal-title":"Lab Invest"},{"key":"10.1016\/j.xfss.2025.07.004_bib17","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/j.fertnstert.2021.05.081","article-title":"Tryptophan catabolism is dysregulated in leiomyomas","volume":"116","author":"Chuang","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfss.2025.07.004_bib18","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.fertnstert.2020.07.024","article-title":"Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis","volume":"115","author":"Chuang","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfss.2025.07.004_bib19","article-title":"Short-term tetrabromobisphenol A exposure promotes fibrosis of human uterine fibroid cells in a 3D culture system through TGF-beta signaling","volume":"36","author":"Liu","year":"2022","journal-title":"FASEB J"},{"key":"10.1016\/j.xfss.2025.07.004_bib20","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/j.fertnstert.2020.06.045","article-title":"Activation of protein kinase B by WNT4 as a regulator of uterine leiomyoma stem cell function","volume":"114","author":"Liu","year":"2020","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfss.2025.07.004_bib21","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1007\/s43032-022-00960-9","article-title":"Human myometrial and uterine fibroid stem cell-derived organoids for intervening the pathophysiology of uterine fibroid","volume":"29","author":"Banerjee","year":"2022","journal-title":"Reprod Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.cellsig.2020.109784","article-title":"Circular RNA expression profiling identifies novel biomarkers in uterine leiomyoma","volume":"76","author":"Wang","year":"2020","journal-title":"Cell Signal"},{"key":"10.1016\/j.xfss.2025.07.004_bib23","doi-asserted-by":"crossref","DOI":"10.1002\/rmb2.12627","article-title":"Establishment of a 3D spheroid culture system to evaluate the responsiveness of uterine leiomyoma cells to female hormones","volume":"24","author":"Sakai","year":"2025","journal-title":"Reprod Med Biol"},{"key":"10.1016\/j.xfss.2025.07.004_bib24","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.70025","article-title":"Utilising human myometrial and uterine fibroid stem cell-derived three dimensional organoids as a robust model system for understanding the pathophysiology of uterine fibroids","author":"Omran","year":"2025","journal-title":"Cell Prolif"},{"key":"10.1016\/j.xfss.2025.07.004_bib25","first-page":"327","article-title":"Uterine fibroid cell cytoskeletal organization is affected by altered G protein-coupled estrogen receptor-1 and phosphatidylinositol 3-kinase signaling","volume":"4","author":"Warwar","year":"2023","journal-title":"F S Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib26","doi-asserted-by":"crossref","DOI":"10.1038\/srep19103","article-title":"3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained","volume":"6","author":"Zanoni","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.xfss.2025.07.004_bib27","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat Methods"},{"key":"10.1016\/j.xfss.2025.07.004_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists","volume":"37","author":"Huang da","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.xfss.2025.07.004_bib29","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape provides a biologist-oriented resource for the analysis of systems-level datasets","volume":"10","author":"Zhou","year":"2019","journal-title":"Nat Commun"},{"key":"10.1016\/j.xfss.2025.07.004_bib30","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: protein-protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.xfss.2025.07.004_bib31","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/pro.4218","article-title":"PANTHER: Making genome-scale phylogenetics accessible to all","volume":"31","author":"Thomas","year":"2022","journal-title":"Protein Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib32","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.xfss.2025.07.004_bib33","article-title":"A systems-based approach to uterine fibroids identifies differential splicing associated with abnormal bleeding","author":"Wang","year":"2024","journal-title":"bioRxiv"},{"key":"10.1016\/j.xfss.2025.07.004_bib34","doi-asserted-by":"crossref","first-page":"3618","DOI":"10.3390\/ijms22073618","article-title":"Transcriptome analyses of myometrium from fibroid patients reveals phenotypic differences compared to non-diseased myometrium","volume":"22","author":"Paul","year":"2021","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib35","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1038\/s41467-020-14701-6","article-title":"Altered chromatin landscape and enhancer engagement underlie transcriptional dysregulation in MED12 mutant uterine leiomyomas","volume":"11","author":"Moyo","year":"2020","journal-title":"Nat Commun"},{"key":"10.1016\/j.xfss.2025.07.004_bib36","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1177\/1933719117711265","article-title":"Expression profiling of lncRNAs, miRNAs, and mRNAs and their differential expression in leiomyoma using next-generation RNA sequencing","volume":"25","author":"Chuang","year":"2018","journal-title":"Reprod Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib37","doi-asserted-by":"crossref","first-page":"D833","DOI":"10.1093\/nar\/gkw943","article-title":"DisGeNET: a comprehensive platform integrating information on human disease-associated genes and variants","volume":"45","author":"Pinero","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.xfss.2025.07.004_bib38","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1002\/pmic.201400213","article-title":"Quantitative proteome profiling of human myoma and myometrium tissue reveals kinase expression signatures with potential for therapeutic intervention","volume":"15","author":"Lemeer","year":"2015","journal-title":"Proteomics"},{"key":"10.1016\/j.xfss.2025.07.004_bib39","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1093\/neuonc\/not128","article-title":"Ligand-dependent EphB1 signaling suppresses glioma invasion and correlates with patient survival","volume":"15","author":"Teng","year":"2013","journal-title":"Neuro Oncol"},{"key":"10.1016\/j.xfss.2025.07.004_bib40","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.7150\/jca.44576","article-title":"Ligand-independent EphB1 signaling mediates TGF-beta-activated CDH2 and promotes lung cancer cell invasion and migration","volume":"11","author":"Wang","year":"2020","journal-title":"J Cancer"},{"key":"10.1016\/j.xfss.2025.07.004_bib41","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.matbio.2012.09.005","article-title":"Integrin beta1 regulates leiomyoma cytoskeletal integrity and growth","volume":"31","author":"Malik","year":"2012","journal-title":"Matrix Biol"},{"key":"10.1016\/j.xfss.2025.07.004_bib42","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1177\/1933719114522548","article-title":"Collagen-binding alpha11 integrin expression in human myometrium and fibroids utilizing a novel RNA in situ probe","volume":"21","author":"Peavey","year":"2014","journal-title":"Reprod Sci"},{"key":"10.1016\/j.xfss.2025.07.004_bib43","doi-asserted-by":"crossref","first-page":"E837","DOI":"10.1210\/jc.2012-3647","article-title":"Overexpression of integrin-beta1 in leiomyoma promotes cell spreading and proliferation","volume":"98","author":"Chen","year":"2013","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1016\/j.xfss.2025.07.004_bib44","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0002-9378(96)70155-2","article-title":"The expression of integrins and cadherins in normal human uterus and uterine leiomyomas","volume":"175","author":"Taylor","year":"1996","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.xfss.2025.07.004_bib45","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1097\/MED.0000000000000578","article-title":"The role of endocrine-disrupting chemicals in uterine fibroid pathogenesis","volume":"27","author":"Bariani","year":"2020","journal-title":"Curr Opin Endocrinol Diabetes Obes"},{"key":"10.1016\/j.xfss.2025.07.004_bib46","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1093\/humrep\/deq034","article-title":"Characteristics indicating adenomyosis coexisting with leiomyomas: a case-control study","volume":"25","author":"Taran","year":"2010","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfss.2025.07.004_bib47","doi-asserted-by":"crossref","first-page":"34","DOI":"10.4103\/0974-1208.82358","article-title":"Do symptomatic endometriosis and uterine fibroids appear together?","volume":"4","author":"Uimari","year":"2011","journal-title":"J Hum Reprod Sci"}],"container-title":["F&S Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666335X25000485?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666335X25000485?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T03:57:29Z","timestamp":1777262249000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666335X25000485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S2666335X25000485"],"URL":"https:\/\/doi.org\/10.1016\/j.xfss.2025.07.004","relation":{},"ISSN":["2666-335X"],"issn-type":[{"value":"2666-335X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Compressive force induces differential gene and protein expression in uterine fibroids","name":"articletitle","label":"Article Title"},{"value":"F&S Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xfss.2025.07.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc. on behalf of American Society for Reproductive Medicine.","name":"copyright","label":"Copyright"}]}}