{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T19:44:27Z","timestamp":1786218267959,"version":"build-2736575974"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["fertstertscience.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["F&amp;S Science"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.xfss.2026.07.003","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:54:08Z","timestamp":1784217248000},"update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["A preliminary time-lapse morphokinetic framework to stratify developmental competence in three-pronuclear zygotes on the basis of pronuclear volume ratios and second polar body extrusion"],"prefix":"10.1016","author":[{"given":"Hayato","family":"Asama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8183-8651","authenticated-orcid":false,"given":"Junko","family":"Otsuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingqiang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noritoshi","family":"Enatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daigaku","family":"Kamibayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Michikura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahide","family":"Shiotani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xfss.2026.07.003_bib1","doi-asserted-by":"crossref","DOI":"10.1002\/rmb2.12462","article-title":"Are tri-pronuclear embryos that show two normal-sized pronuclei and additional smaller pronuclei useful for embryo transfer?","volume":"21","author":"Takahashi","year":"2022","journal-title":"Reprod Med Biol"},{"key":"10.1016\/j.xfss.2026.07.003_bib2","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1007\/s10815-024-03114-9","article-title":"The utility of human two plus one small pronucleated zygotes (2.1PN) based on clinical outcomes and the focused ploidy analysis","volume":"41","author":"Hattori","year":"2024","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.xfss.2026.07.003_bib3","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1007\/s10815-024-03113-w","article-title":"Evaluating the developmental potential of 2.1PN-derived embryos and associated chromosomal analysis","volume":"41","author":"Wang","year":"2024","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.xfss.2026.07.003_bib4","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.fertnstert.2017.08.004","article-title":"Abnormally fertilized oocytes can result in healthy live births: improved genetic technologies for preimplantation genetic testing can be used to rescue viable embryos in in vitro fertilization cycles","volume":"108","author":"Capalbo","year":"2017","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfss.2026.07.003_bib5","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3390\/biomedicines13010051","article-title":"The identification of molecular ploidy status of abnormal pronuclear zygotes reveals a significant number of euploid blastocysts available for conception","volume":"13","author":"McCallie","year":"2024","journal-title":"Biomedicines"},{"key":"10.1016\/j.xfss.2026.07.003_bib24","series-title":"The myth of self-correction: passive selection in early embryos and nuclear reorganization in diploid 3PN zygotes","author":"Otsuki","year":"2025"},{"key":"10.1016\/j.xfss.2026.07.003_bib6","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1093\/humrep\/deab064","article-title":"The true incidence of chromosomal mosaicism after preimplantation genetic testing is much lower than that indicated by trophectoderm biopsy","volume":"36","author":"Wu","year":"2021","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfss.2026.07.003_bib7","first-page":"492","article-title":"Detecting mosaicism in trophectoderm biopsies: current challenges and future possibilities","volume":"32","author":"Capalbo","year":"2017","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfss.2026.07.003_bib8","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.fertnstert.2019.03.033","article-title":"Maternal and neonatal outcomes associated with trophectoderm biopsy","volume":"112","author":"Zhang","year":"2019","journal-title":"Fertil Steril"},{"issue":"36","key":"10.1016\/j.xfss.2026.07.003_bib9","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1093\/humrep\/deaa316","article-title":"Impact of trophectoderm biopsy on obstetric and perinatal outcomes following frozen-thawed embryo transfer cycles","volume":"25","author":"Makhijani","year":"2021","journal-title":"Hum Reprod"},{"key":"10.1016\/j.xfss.2026.07.003_bib10","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1007\/s10815-023-02830-y","article-title":"Assessing the clinical viability of micro 3 pronuclei zygotes","volume":"40","author":"Canon","year":"2023","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.xfss.2026.07.003_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.rbmo.2024.104595","article-title":"Reassessing the conventional fertilization check: leveraging preimplantation genetic testing for aneuploidy to increase the number of transferrable embryos","volume":"50","author":"Al Hashimi","year":"2025","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.xfss.2026.07.003_bib12","article-title":"Use of time-lapse technology on fertilization verification, embryo evaluation, and utilization: a national survey in Japan","volume":"4","author":"Yamada","year":"2024","journal-title":"Am J Obstet Gynecol Glob Rep"},{"key":"10.1016\/j.xfss.2026.07.003_bib13","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1017\/S096719942100040X","article-title":"Nuclear-to-cytoplasmic ratios of 1PN and 2PN zygotes after in vitro fertilization of mouse oocytes","volume":"30","author":"Okajima","year":"2022","journal-title":"Zygote"},{"key":"10.1016\/j.xfss.2026.07.003_bib14","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s10815-024-03312-5","article-title":"Correlation between female pronuclear\/cytoplasmic ratio and number of chromosomes in mouse zygotic stage: implications for aneuploidy assessment in ART","volume":"42","author":"Xiao","year":"2025","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.xfss.2026.07.003_bib15","doi-asserted-by":"crossref","DOI":"10.1002\/rmb2.12578","article-title":"Morphokinetic features in human embryos: analysis by our original high-resolution time-lapse cinematography-Summary of the past two decades","volume":"23","author":"Mio","year":"2024","journal-title":"Reprod Med Biol"},{"key":"10.1016\/j.xfss.2026.07.003_bib16","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1002\/rmb2.12032","article-title":"Potential of zygotes to produce live births can be identified by the size of the male and female pronuclei just before their membranes break down","volume":"16","author":"Otsuki","year":"2017","journal-title":"Reprod Med Biol"},{"key":"10.1016\/j.xfss.2026.07.003_bib17","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1016\/j.fertnstert.2019.07.015","article-title":"Noninvasive embryo selection: kinetic analysis of female and male pronuclear development to predict embryo quality and potential to produce live birth","volume":"112","author":"Otsuki","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.xfss.2026.07.003_bib18","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1530\/rep.0.1250625","article-title":"Remodelling the paternal chromatin at fertilization in mammals","volume":"125","author":"McLay","year":"2003","journal-title":"Reproduction"},{"issue":"5","key":"10.1016\/j.xfss.2026.07.003_bib19","doi-asserted-by":"crossref","first-page":"5868","DOI":"10.1038\/ncomms6868","article-title":"Paternal heterochromatin formation in human embryos is H3K9\/HP1 directed and primed by sperm-derived histone modifications","volume":"18","author":"van de Werken","year":"2014","journal-title":"Nat Commun"},{"key":"10.1016\/j.xfss.2026.07.003_bib20","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.devcel.2014.06.022","article-title":"Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote","volume":"30","author":"Lin","year":"2014","journal-title":"Dev Cell"},{"key":"10.1016\/j.xfss.2026.07.003_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0202663","article-title":"Single nucleolus precursor body formation in the pronucleus of mouse zygotes and SCNT embryos","volume":"13","author":"Kyogoku","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.xfss.2026.07.003_bib22","doi-asserted-by":"crossref","first-page":"660.e1","DOI":"10.1016\/j.ajog.2008.07.023","article-title":"Time-lapse cinematography of dynamic changes occurring during in vitro development of human embryos","volume":"199","author":"Mio","year":"2008","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.xfss.2026.07.003_bib23","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1242\/dev.01116","article-title":"Regulation of histone H3 lysine 9 methylation in oocytes and early pre-implantation embryos","volume":"131","author":"Liu","year":"2004","journal-title":"Development"}],"container-title":["F&amp;S Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666335X26000510?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666335X26000510?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T19:34:04Z","timestamp":1786217644000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666335X26000510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":24,"alternative-id":["S2666335X26000510"],"URL":"https:\/\/doi.org\/10.1016\/j.xfss.2026.07.003","relation":{},"ISSN":["2666-335X"],"issn-type":[{"value":"2666-335X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A preliminary time-lapse morphokinetic framework to stratify developmental competence in three-pronuclear zygotes on the basis of pronuclear volume ratios and second polar body extrusion","name":"articletitle","label":"Article Title"},{"value":"F&S Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xfss.2026.07.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}