{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:31:44Z","timestamp":1790299904879,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["jpharmsci.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical Sciences"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1016\/j.xphs.2017.02.024","type":"journal-article","created":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T11:45:29Z","timestamp":1488368729000},"page":"1490-1498","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Feasibility of Freeze-Drying Oil-in-Water Emulsion Adjuvants and Subunit Proteins to Enable Single-Vial Vaccine Drug Products"],"prefix":"10.1016","volume":"106","author":[{"given":"Vidyashankara","family":"Iyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Cayatte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason D.","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Schneider-Ohrum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean K.","family":"Maynard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav Manohar","family":"Rajani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angie Snell","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Richard L.","family":"Remmele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve M.","family":"Bishop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"McCarthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilikallahalli K.","family":"Muralidhara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Suppl 3","key":"10.1016\/j.xphs.2017.02.024_bib1","doi-asserted-by":"crossref","first-page":"C25","DOI":"10.1016\/j.vaccine.2010.07.021","article-title":"Unmet needs in modern vaccinology: adjuvants to improve the immune response","volume":"28","author":"Leroux-Roels","year":"2010","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.xphs.2017.02.024_bib2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1586\/erv.11.192","article-title":"Development and evaluation of AS03, an Adjuvant System containing alpha-tocopherol and squalene in an oil-in-water emulsion","volume":"11","author":"Garcon","year":"2012","journal-title":"Expert Rev Vaccin"},{"issue":"4","key":"10.1016\/j.xphs.2017.02.024_bib3","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1586\/erv.09.5","article-title":"Emulsion-based adjuvants for influenza vaccines","volume":"8","author":"Vogel","year":"2009","journal-title":"Expert Rev Vaccin"},{"issue":"25-26","key":"10.1016\/j.xphs.2017.02.024_bib4","first-page":"3464","article-title":"Experimental squalene adjuvant. I. Preparation and testing of its effectiveness","volume":"22","author":"Suli","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2017.02.024_bib5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/978-1-4615-1823-5_10","article-title":"MF59. Design and evaluation of a safe and potent adjuvant for human vaccines","volume":"6","author":"Ott","year":"1995","journal-title":"Pharm Biotechnol"},{"issue":"2","key":"10.1016\/j.xphs.2017.02.024_bib6","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1586\/14760584.2.2.197","article-title":"MF59-adjuvanted vaccines: increased immunogenicity with an optimal safety profile","volume":"2","author":"Podda","year":"2003","journal-title":"Expert Rev Vaccin"},{"issue":"3","key":"10.1016\/j.xphs.2017.02.024_bib7","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1093\/infdis\/jir769","article-title":"GLA-SE, a synthetic toll-like receptor 4 agonist, enhances T-cell responses to influenza vaccine in older adults","volume":"205","author":"Behzad","year":"2012","journal-title":"J\u00a0Infect Dis"},{"issue":"1","key":"10.1016\/j.xphs.2017.02.024_bib8","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1006\/meth.1999.0834","article-title":"Monophosphoryl lipid A (MPL) formulations for the next generation of vaccines","volume":"19","author":"Baldridge","year":"1999","journal-title":"Methods"},{"issue":"6","key":"10.1016\/j.xphs.2017.02.024_bib9","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1007\/s11095-009-9859-5","article-title":"MF59 emulsion is an effective delivery system for a synthetic TLR4 agonist (E6020)","volume":"26","author":"Baudner","year":"2009","journal-title":"Pharm Res"},{"issue":"12","key":"10.1016\/j.xphs.2017.02.024_bib10","doi-asserted-by":"crossref","first-page":"e51618","DOI":"10.1371\/journal.pone.0051618","article-title":"Molecular and cellular response profiles induced by the TLR4 agonist-based adjuvant Glucopyranosyl Lipid A","volume":"7","author":"Lambert","year":"2012","journal-title":"PLoS One"},{"issue":"17","key":"10.1016\/j.xphs.2017.02.024_bib11","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1016\/j.vaccine.2010.08.002","article-title":"TLR-based immune adjuvants","volume":"29","author":"Steinhagen","year":"2011","journal-title":"Vaccine"},{"issue":"7","key":"10.1016\/j.xphs.2017.02.024_bib12","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1080\/21645515.2015.1046660","article-title":"Impact of formulation and particle size on stability and immunogenicity of oil-in-water emulsion adjuvants","volume":"11","author":"Iyer","year":"2015","journal-title":"Hum Vaccin Immunother"},{"key":"10.1016\/j.xphs.2017.02.024_bib13","series-title":"Vaccine Adjuvants","first-page":"211","article-title":"The adjuvant MF59: a 10-year perspective","author":"Gary Ott","year":"2000"},{"key":"10.1016\/j.xphs.2017.02.024_bib14","series-title":"Vaccine design: the subunit and adjuvant approach","first-page":"277","author":"Gary Ott","year":"1995"},{"issue":"1","key":"10.1016\/j.xphs.2017.02.024_bib15","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/jps.24705","article-title":"Freeze-drying above the glass transition temperature in amorphous protein formulations while maintaining product quality and improving process efficiency","volume":"105","author":"Depaz","year":"2016","journal-title":"J\u00a0Pharm Sci"},{"issue":"48","key":"10.1016\/j.xphs.2017.02.024_bib16","doi-asserted-by":"crossref","first-page":"6771","DOI":"10.1016\/j.vaccine.2015.10.024","article-title":"Identification of the critical attribute(s) of EBV gp350 antigen required for elicitation of a neutralizing antibody response in\u00a0vivo","volume":"33","author":"Servat","year":"2015","journal-title":"Vaccine"},{"issue":"23","key":"10.1016\/j.xphs.2017.02.024_bib17","doi-asserted-by":"crossref","first-page":"2562","DOI":"10.1016\/j.vaccine.2016.04.012","article-title":"Identification of GLA\/SE as an effective adjuvant for the induction of robust humoral and cell-mediated immune responses to EBV-gp350 in mice and rabbits","volume":"34","author":"Heeke","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2017.02.024_bib18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1742-4933-9-21","article-title":"An adjuvanted respiratory syncytial virus fusion protein induces protection in aged BALB\/c mice","volume":"9","author":"Cherukuri","year":"2012","journal-title":"Immun Ageing"},{"issue":"7","key":"10.1016\/j.xphs.2017.02.024_bib19","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1128\/jvi.63.7.2941-2950.1989","article-title":"Neutralization epitopes of the F-Glycoprotein of respiratory syncytial virus\u2014effect of mutation upon fusion function","volume":"63","author":"Beeler","year":"1989","journal-title":"J\u00a0Virol"},{"issue":"20","key":"10.1016\/j.xphs.2017.02.024_bib20","doi-asserted-by":"crossref","first-page":"11107","DOI":"10.1128\/JVI.01554-13","article-title":"Cytomegalovirus vaccine strain towne-derived dense bodies induce broad cellular immune responses and neutralizing antibodies that prevent infection of fibroblasts and epithelial cells","volume":"87","author":"Cayatte","year":"2013","journal-title":"J\u00a0Virol"},{"issue":"10","key":"10.1016\/j.xphs.2017.02.024_bib21","doi-asserted-by":"crossref","first-page":"e13677","DOI":"10.1371\/journal.pone.0013677","article-title":"A\u00a0synthetic adjuvant to enhance and\u00a0expand immune responses to influenza vaccines","volume":"5","author":"Coler","year":"2010","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.xphs.2017.02.024_bib22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/eji.201141855","article-title":"A\u00a0new synthetic TLR4 agonist, GLA, allows dendritic cells targeted with antigen to elicit Th1 T-cell immunity in\u00a0vivo","volume":"42","author":"Pantel","year":"2012","journal-title":"Eur J Immunol"},{"issue":"3","key":"10.1016\/j.xphs.2017.02.024_bib23","doi-asserted-by":"crossref","first-page":"e0119509","DOI":"10.1371\/journal.pone.0119509","article-title":"A\u00a0novel respiratory syncytial virus (RSV) F subunit vaccine adjuvanted with GLA-SE elicits robust protective T(H)1-type humoral and cellular immunity in rodent models","volume":"10","author":"Lambert","year":"2015","journal-title":"PLoS One"},{"issue":"8","key":"10.1016\/j.xphs.2017.02.024_bib24","doi-asserted-by":"crossref","first-page":"4254","DOI":"10.4049\/jimmunol.167.8.4254","article-title":"Visualization and characterization of respiratory syncytial virus F-specific CD8(+) T cells during experimental virus infection","volume":"167","author":"Chang","year":"2001","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.xphs.2017.02.024_bib25","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jconrel.2013.12.025","article-title":"Elimination of the cold-chain dependence of a nanoemulsion adjuvanted vaccine against tuberculosis by lyophilization","volume":"177","author":"Orr","year":"2014","journal-title":"J\u00a0Control Release"},{"key":"10.1016\/j.xphs.2017.02.024_bib26","first-page":"1","article-title":"Lyophilization of an adjuvanted Mycobacterium tuberculosis vaccine in a single-chamber pharmaceutical cartridge","author":"Barnes","year":"2016","journal-title":"AAPS PharmSciTech"},{"issue":"12","key":"10.1016\/j.xphs.2017.02.024_bib27","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1023\/A:1021445608807","article-title":"Effects of sucrose and trehalose on the preservation of the native structure of spray-dried lysozyme","volume":"19","author":"Liao","year":"2002","journal-title":"Pharm Res"},{"issue":"12","key":"10.1016\/j.xphs.2017.02.024_bib28","doi-asserted-by":"crossref","first-page":"5106","DOI":"10.1002\/jps.21374","article-title":"Solid state chemistry of proteins: II. The correlation of storage stability of freeze-dried human growth hormone (hGH) with structure and dynamics in the glassy solid","volume":"97","author":"Pikal","year":"2008","journal-title":"J\u00a0Pharm Sci"},{"issue":"1","key":"10.1016\/j.xphs.2017.02.024_bib29","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0378-5173(98)00024-6","article-title":"Formulation of a lyophilized dry emulsion tablet for the delivery of poorly soluble drugs","volume":"166","author":"Corveleyn","year":"1998","journal-title":"Int J Pharm"},{"key":"10.1016\/j.xphs.2017.02.024_bib30","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.colsurfa.2013.07.015","article-title":"Freezing and freeze-drying of Pickering emulsions stabilized by starch granules","volume":"436","author":"Marefati","year":"2013","journal-title":"Colloids Surf A Physicochem Eng Aspects"},{"issue":"1","key":"10.1016\/j.xphs.2017.02.024_bib31","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.colsurfa.2011.09.048","article-title":"Self-nanoemulsifying drug delivery system for adefovir dipivoxil: design, characterization, in\u00a0vitro and ex\u00a0vivo evaluation","volume":"392","author":"Gupta","year":"2011","journal-title":"Colloids Surf A Physicochem Eng Aspects"}],"container-title":["Journal of Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022354917301338?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022354917301338?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T09:22:53Z","timestamp":1758360173000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022354917301338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["S0022354917301338"],"URL":"https:\/\/doi.org\/10.1016\/j.xphs.2017.02.024","relation":{},"ISSN":["0022-3549"],"issn-type":[{"value":"0022-3549","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Feasibility of Freeze-Drying Oil-in-Water Emulsion Adjuvants and Subunit Proteins to Enable Single-Vial Vaccine Drug Products","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xphs.2017.02.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 American Pharmacists Association\u00ae. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}