{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T10:45:14Z","timestamp":1774521914966,"version":"3.50.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100008617","name":"Danish National Advanced Technology Foundation","doi-asserted-by":"publisher","award":["069-2011-1"],"award-info":[{"award-number":["069-2011-1"]}],"id":[{"id":"10.13039\/100008617","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jpharmsci.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical Sciences"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.xphs.2018.02.005","type":"journal-article","created":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T19:09:27Z","timestamp":1518548967000},"page":"1690-1700","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Unusual Self-Assembly of the Recombinant Chlamydia trachomatis Major Outer Membrane Protein\u2013Based Fusion Antigen CTH522 Into Protein Nanoparticles"],"prefix":"10.1016","volume":"107","author":[{"given":"Fabrice","family":"Rose","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasper","family":"Karlsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernille R\u00f8nde","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasmus Uffe","family":"Jakobsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grith Kr\u00f8yer","family":"Wood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasper Dyrberg","family":"Rand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helene","family":"Godiksen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Follmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilla","family":"Foged","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xphs.2018.02.005_bib1","doi-asserted-by":"crossref","first-page":"2664","DOI":"10.4161\/hv.29683","article-title":"Immunity, immunopathology, and human vaccine development against sexually transmitted Chlamydia trachomatis","volume":"10","author":"Rey-Ladino","year":"2014","journal-title":"Hum Vaccines Immunother"},{"key":"10.1016\/j.xphs.2018.02.005_bib2","series-title":"Global Incidence and Prevalence of Selected Curable Sexually Transmitted Infections\u20132008","author":"WHO","year":"2012"},{"key":"10.1016\/j.xphs.2018.02.005_bib3","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/nrmicro.2016.30","article-title":"Chlamydia cell biology and pathogenesis","volume":"14","author":"Elwell","year":"2016","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.xphs.2018.02.005_bib4","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/nri1551","article-title":"Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine","volume":"5","author":"Brunham","year":"2005","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.xphs.2018.02.005_bib5","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1016\/j.vaccine.2013.01.024","article-title":"Chlamydia trachomatis control requires a vaccine","volume":"31","author":"Brunham","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2018.02.005_bib6","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1016\/j.vaccine.2013.10.016","article-title":"Towards a safe and effective chlamydial vaccine: lessons from the eye","volume":"32","author":"Mabey","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2018.02.005_bib7","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1093\/infdis\/jiv137","article-title":"Protection against Chlamydia trachomatis infection and upper genital tract pathological changes by vaccine-promoted neutralizing antibodies directed to the VD4 of the major outer membrane protein","volume":"212","author":"Olsen","year":"2015","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.xphs.2018.02.005_bib8","doi-asserted-by":"crossref","first-page":"628","DOI":"10.3389\/fimmu.2015.00628","article-title":"Intramuscular priming and intranasal boosting induce strong genital immunity through secretory IgA in minipigs infected with Chlamydia trachomatis","volume":"6","author":"Lorenzen","year":"2015","journal-title":"Front Immunol"},{"key":"10.1016\/j.xphs.2018.02.005_bib9","doi-asserted-by":"crossref","first-page":"6240","DOI":"10.1128\/IAI.69.10.6240-6247.2001","article-title":"Immunization with the Chlamydia trachomatis mouse pneumonitis major outer membrane protein can elicit a protective immune response against a genital challenge","volume":"69","author":"Pal","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.xphs.2018.02.005_bib10","doi-asserted-by":"crossref","first-page":"5020","DOI":"10.1016\/j.vaccine.2009.05.008","article-title":"Protection against an intranasal challenge by vaccines formulated with native and recombinant preparations of the Chlamydia trachomatis major outer membrane protein","volume":"27","author":"Sun","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2018.02.005_bib11","doi-asserted-by":"crossref","first-page":"8063","DOI":"10.4049\/jimmunol.0804375","article-title":"Chlamydia trachomatis native major outer membrane protein induces partial protection in nonhuman primates: implication for a trachoma transmission-blocking vaccine","volume":"182","author":"Kari","year":"2009","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.xphs.2018.02.005_bib12","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1128\/MMBR.67.4.593-656.2003","article-title":"Molecular basis of bacterial outer membrane permeability revisited","volume":"67","author":"Nikaido","year":"2003","journal-title":"Microbiol Mol Biol Rev"},{"key":"10.1016\/j.xphs.2018.02.005_bib13","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1021\/mp900110q","article-title":"Biophysical and stabilization studies of the Chlamydia trachomatis mouse pneumonitis major outer membrane protein","volume":"6","author":"Cai","year":"2009","journal-title":"Mol Pharm"},{"key":"10.1016\/j.xphs.2018.02.005_bib14","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1128\/jb.169.9.3879-3885.1987","article-title":"Diversity of Chlamydia trachomatis major outer membrane protein genes","volume":"169","author":"Stephens","year":"1987","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.xphs.2018.02.005_bib15","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1073\/pnas.85.11.4000","article-title":"Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes","volume":"85","author":"Baehr","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.xphs.2018.02.005_bib16","first-page":"892","article-title":"Phylogenetic analysis of the outer-membrane-protein genes of Chlamydiae, and its implication for vaccine development","volume":"10","author":"Fitch","year":"1993","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.xphs.2018.02.005_bib17","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1084\/jem.167.3.817","article-title":"High-resolution mapping of serovar-specific and common antigenic determinants of the major outer membrane protein of Chlamydia trachomatis","volume":"167","author":"Stephens","year":"1988","journal-title":"J\u00a0Exp Med"},{"key":"10.1016\/j.xphs.2018.02.005_bib18","doi-asserted-by":"crossref","first-page":"5276","DOI":"10.1016\/j.vaccine.2011.05.013","article-title":"Induction of protection against vaginal shedding and infertility by a recombinant Chlamydia vaccine","volume":"29","author":"Carmichael","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2018.02.005_bib19","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0264-410X(98)00219-9","article-title":"Vaccination of mice with DNA plasmids coding for the Chlamydia trachomatis major outer membrane protein elicits an immune response but fails to protect against a genital challenge","volume":"17","author":"Pal","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.xphs.2018.02.005_bib20","doi-asserted-by":"crossref","first-page":"6250","DOI":"10.1021\/bi047775v","article-title":"Characterization of the disulfide bonds and free cysteine residues of the Chlamydia trachomatis mouse pneumonitis major outer membrane protein","volume":"44","author":"Yen","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.xphs.2018.02.005_bib21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jconrel.2015.05.004","article-title":"Engineering of a novel adjuvant based on lipid-polymer hybrid nanoparticles: a quality-by-design approach","volume":"210","author":"Rose","year":"2015","journal-title":"J\u00a0Control Release"},{"key":"10.1016\/j.xphs.2018.02.005_bib22","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/0076-6879(86)31045-0","article-title":"Determination and analysis of urea and guanidine hydrochloride denaturation curves","volume":"131","author":"Pace","year":"1986","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.xphs.2018.02.005_bib23","first-page":"177","article-title":"Urea and guanidine hydrochloride denaturation curves","volume":"40","author":"Shirley","year":"1995","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.xphs.2018.02.005_bib24","doi-asserted-by":"crossref","first-page":"6222","DOI":"10.1128\/JB.00552-07","article-title":"Structural and functional analyses of the major outer membrane protein of Chlamydia trachomatis","volume":"189","author":"Sun","year":"2007","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.xphs.2018.02.005_bib25","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1007\/s11095-007-9516-9","article-title":"Extrinsic fluorescent dyes as tools for protein characterization","volume":"25","author":"Hawe","year":"2008","journal-title":"Pharm Res"},{"key":"10.1016\/j.xphs.2018.02.005_bib26","doi-asserted-by":"crossref","first-page":"16424","DOI":"10.1021\/bi991765q","article-title":"Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques","volume":"38","author":"Wilkins","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.xphs.2018.02.005_bib27","doi-asserted-by":"crossref","first-page":"17876","DOI":"10.1073\/pnas.1114477108","article-title":"Physical limits of cells and proteomes","volume":"108","author":"Dill","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.xphs.2018.02.005_bib28","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s12575-009-9008-x","article-title":"Size and shape of protein molecules at the nanometer level determined by sedimentation, gel filtration, and electron microscopy","volume":"11","author":"Erickson","year":"2009","journal-title":"Biol Proced Online"},{"key":"10.1016\/j.xphs.2018.02.005_bib29","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.jcis.2009.10.048","article-title":"Colloidal dispersion stability of unilamellar DPPC vesicles in aqueous electrolyte solutions and comparisons to predictions of the DLVO theory","volume":"342","author":"Park","year":"2010","journal-title":"J\u00a0Colloid Interf Sci"},{"key":"10.1016\/j.xphs.2018.02.005_bib30","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/jcis.2000.7003","article-title":"On the applicability of DLVO theory to the prediction of clay colloids stability","volume":"230","author":"Missana","year":"2000","journal-title":"J\u00a0Colloid Interface Sci"},{"key":"10.1016\/j.xphs.2018.02.005_bib31","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1007\/s11095-012-0856-8","article-title":"Protein antigen adsorption to the DDA\/TDB liposomal adjuvant: effect on protein structure, stability, and liposome physicochemical characteristics","volume":"30","author":"Hamborg","year":"2013","journal-title":"Pharm Res"},{"key":"10.1016\/j.xphs.2018.02.005_bib32","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1471-2180-5-5","article-title":"Surface expression, single-channel analysis and membrane topology of recombinant Chlamydia trachomatis Major Outer Membrane Protein","volume":"5","author":"Findlay","year":"2005","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.xphs.2018.02.005_bib33","first-page":"681","article-title":"SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments","volume":"11","author":"Geourjon","year":"1995","journal-title":"Comput Appl Biosci"},{"key":"10.1016\/j.xphs.2018.02.005_bib34","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/1472-6807-9-51","article-title":"A\u00a0generic method for assignment of reliability scores applied to solvent accessibility predictions","volume":"9","author":"Petersen","year":"2009","journal-title":"BMC Struct Biol"},{"key":"10.1016\/j.xphs.2018.02.005_bib35","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1126\/science.282.5389.754","article-title":"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis","volume":"282","author":"Stephens","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.xphs.2018.02.005_bib36","doi-asserted-by":"crossref","first-page":"W400","DOI":"10.1093\/nar\/gkh417","article-title":"PRED-TMBB: a web server for predicting the topology of beta-barrel outer membrane proteins","volume":"32","author":"Bagos","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.xphs.2018.02.005_bib37","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2105-6-7","article-title":"Evaluation of methods for predicting the topology of beta-barrel outer membrane proteins and a consensus prediction method","volume":"6","author":"Bagos","year":"2005","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.xphs.2018.02.005_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1046-5928(02)00641-1","article-title":"Practical considerations in refolding proteins from inclusion bodies","volume":"28","author":"Tsumoto","year":"2003","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.xphs.2018.02.005_bib39","article-title":"Recent advances in self-assembled peptides: implications for targeted drug delivery and vaccine engineering","author":"Eskandari","year":"2016","journal-title":"Adv Drug Delivery Rev"},{"key":"10.1016\/j.xphs.2018.02.005_bib40","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.1039\/C3RA45944F","article-title":"Rational design of self-assembled proteins and peptides for nano- and micro-sized architectures","volume":"4","author":"Matsuurua","year":"2014","journal-title":"RSC Adv"}],"container-title":["Journal of Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002235491830087X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002235491830087X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T09:23:40Z","timestamp":1758360220000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002235491830087X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["S002235491830087X"],"URL":"https:\/\/doi.org\/10.1016\/j.xphs.2018.02.005","relation":{},"ISSN":["0022-3549"],"issn-type":[{"value":"0022-3549","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Unusual Self-Assembly of the Recombinant Chlamydia trachomatis Major Outer Membrane Protein\u2013Based Fusion Antigen CTH522 Into Protein Nanoparticles","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xphs.2018.02.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 American Pharmacists Association\u00ae. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}