{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:31:59Z","timestamp":1780637519669,"version":"3.54.1"},"reference-count":6,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T00:00:00Z","timestamp":1596153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006058","name":"St. Baldrick's Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006058","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.xpro.2020.100090","type":"journal-article","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T07:50:07Z","timestamp":1598255407000},"page":"100090","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Processing Human Thymic Tissue for Single Cell RNA-Seq"],"prefix":"10.1016","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4275-7725","authenticated-orcid":false,"given":"Justin","family":"Le","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vi Luan","family":"Ha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Luong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chintan","family":"Parekh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2020.100090_bib1","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1038\/ni.3299","article-title":"Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T\u00a0cell lineages","volume":"16","author":"Casero","year":"2015","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.xpro.2020.100090_bib2","doi-asserted-by":"crossref","first-page":"2503","DOI":"10.1038\/leu.2017.70","article-title":"The T-ALL related gene BCL11B regulates the initial stages of human T-cell differentiation","volume":"31","author":"Ha","year":"2017","journal-title":"Leukemia"},{"key":"10.1016\/j.xpro.2020.100090_bib3","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1182\/blood-2007-08-106294","article-title":"Human intrathymic lineage\u00a0commitment is marked by differential CD7 expression: identification of CD7- lympho-myeloid thymic progenitors","volume":"111","author":"Hao","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.xpro.2020.100090_bib4","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/nri3667","article-title":"Positive and negative selection of the T\u00a0cell repertoire: what thymocytes see (and don't see)","volume":"14","author":"Klein","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.xpro.2020.100090_bib5","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.immuni.2020.05.010","article-title":"Single-cell RNA-Seq mapping of human thymopoiesis reveals lineage specification trajectories and a commitment spectrum in T\u00a0cell development","volume":"52","author":"Le","year":"2020","journal-title":"Immunity"},{"key":"10.1016\/j.xpro.2020.100090_bib6","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s00281-008-0135-2","article-title":"Human intrathymic development: a selective approach","volume":"30","author":"Plum","year":"2008","journal-title":"Semin. Immunopathol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166720300770?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166720300770?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T19:35:27Z","timestamp":1762976127000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166720300770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":6,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["S2666166720300770"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2020.100090","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Processing Human Thymic Tissue for Single Cell RNA-Seq","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2020.100090","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.immuni.2020.05.010","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"100090"}}