{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T23:32:50Z","timestamp":1784849570647,"version":"3.55.0"},"reference-count":6,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001794","name":"Pediatric Brain Tumor Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008455","name":"Medical School, University of Michigan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008455","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010612","name":"ChadTough Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010612","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017065","name":"Rogel Cancer Center, University of Michigan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["1R21NS107894"],"award-info":[{"award-number":["1R21NS107894"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01-NS076991"],"award-info":[{"award-number":["R01-NS076991"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01-NS096756"],"award-info":[{"award-number":["R01-NS096756"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01-NS105556"],"award-info":[{"award-number":["R01-NS105556"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R37-NS094804"],"award-info":[{"award-number":["R37-NS094804"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.xpro.2020.100165","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T09:30:39Z","timestamp":1606296639000},"page":"100165","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genetically Engineered Mouse Model of Brainstem High-Grade Glioma"],"prefix":"10.1016","volume":"1","author":[{"given":"Fernando M.","family":"Nunez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica C.","family":"Gauss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flor M.","family":"Mendez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Haase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro R.","family":"Lowenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2020.100165_bib1","first-page":"52443","article-title":"Transposon mediated integration of plasmid DNA into the subventricular zone of neonatal mice to generate novel models of glioblastoma","volume":"2015","author":"Calinescu","year":"2015","journal-title":"J.\u00a0Vis. Exp."},{"key":"10.1016\/j.xpro.2020.100165_bib2","doi-asserted-by":"crossref","first-page":"328ra28","DOI":"10.1126\/scitranslmed.aac8228","article-title":"ATRX loss promotes tumor growth and impairs nonhomologous end joining DNA repair in glioma","volume":"8","author":"Koschmann","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.xpro.2020.100165_bib3","doi-asserted-by":"crossref","first-page":"4080","DOI":"10.1158\/1078-0432.CCR-19-3714","article-title":"Therapeutic efficacy of immune stimulatory thymidine kinase and fms-like Tyrosine kinase 3 ligand (TK\/Flt3L) gene therapy in a mouse model of high-grade brainstem glioma","volume":"26","author":"Mendez","year":"2020","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.xpro.2020.100165_bib4","doi-asserted-by":"crossref","first-page":"eaaq1427","DOI":"10.1126\/scitranslmed.aaq1427","article-title":"IDH1-R132H acts as a tumor suppressor in glioma via epigenetic up-regulation of the DNA damage response","volume":"11","author":"Nunez","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.xpro.2020.100165_bib5","doi-asserted-by":"crossref","first-page":"11003","DOI":"10.1038\/s41598-020-67411-w","article-title":"GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma models","volume":"10","author":"Sanchez","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2020.100165_bib6","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1158\/0008-5472.CAN-08-1800","article-title":"De novo induction of genetically engineered brain tumors in mice using plasmid DNA","volume":"69","author":"Wiesner","year":"2009","journal-title":"Cancer Res."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166720301520?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166720301520?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T15:47:00Z","timestamp":1777045620000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166720301520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":6,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["S2666166720301520"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2020.100165","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genetically Engineered Mouse Model of Brainstem High-Grade Glioma","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2020.100165","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"100165"}}