{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:48:58Z","timestamp":1788349738042,"version":"build-2803163510"},"reference-count":8,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004385","name":"Universiteit Gent","doi-asserted-by":"publisher","award":["VIB-GC01-C01"],"award-info":[{"award-number":["VIB-GC01-C01"]}],"id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.xpro.2021.100351","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T17:35:22Z","timestamp":1613928922000},"page":"100351","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":35,"title":["Analysis of T\u00a0cells in mouse lymphoid tissue and blood with flow cytometry"],"prefix":"10.1016","volume":"2","author":[{"given":"Ioannis","family":"Skordos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4782-634X","authenticated-orcid":false,"given":"Annelies","family":"Demeyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5704-582X","authenticated-orcid":false,"given":"Rudi","family":"Beyaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2021.100351_bib1","doi-asserted-by":"crossref","first-page":"6467","DOI":"10.4049\/jimmunol.180.10.6467","article-title":"Cutting edge: expression of TNFR2 defines a maximally suppressive subset of mouse CD4\u00a0+ CD25\u00a0+ FoxP3\u00a0+ T regulatory cells: applicability to tumor-infiltrating T regulatory cells","volume":"180","author":"Chen","year":"2008","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.xpro.2021.100351_bib2","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1002\/eji.201970107","article-title":"Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)","volume":"49","author":"Cossarizza","year":"2019","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.xpro.2021.100351_bib3","doi-asserted-by":"crossref","first-page":"101557","DOI":"10.1016\/j.isci.2020.101557","article-title":"Long-term MALT1 inhibition in adult mice without severe systemic autoimmunity","volume":"23","author":"Demeyer","year":"2020","journal-title":"iScience"},{"key":"10.1016\/j.xpro.2021.100351_bib4","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/s41586-019-1215-2","article-title":"Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy","volume":"570","author":"Di Pilato","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2021.100351_bib5","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1177\/0023677216653984","article-title":"Does age matter? the impact of rodent age on study outcomes","volume":"51","author":"Jackson","year":"2017","journal-title":"Lab. Anim."},{"key":"10.1016\/j.xpro.2021.100351_bib6","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/imm.12754","article-title":"CTLA-4 expressed by FOXP3+ regulatory T\u00a0cells prevents inflammatory tissue attack and not T-cell priming in arthritis","volume":"152","author":"Klocke","year":"2017","journal-title":"Immunology"},{"key":"10.1016\/j.xpro.2021.100351_bib7","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/s41590-018-0289-6","article-title":"Thymic regulatory T\u00a0cells arise via two distinct developmental programs","volume":"20","author":"Owen","year":"2019","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.xpro.2021.100351_bib8","first-page":"1","article-title":"Bcl10 is required for the development and suppressive function of Foxp3+ regulatory T\u00a0cells","volume":"1","author":"Yang","year":"2019","journal-title":"Cell. Mol. Immunol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166721000587?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166721000587?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T19:45:11Z","timestamp":1762976711000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166721000587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["S2666166721000587"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2021.100351","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Analysis of T\u00a0cells in mouse lymphoid tissue and blood with flow cytometry","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2021.100351","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2020.101557","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"100351"}}