{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:57:18Z","timestamp":1790927838313,"version":"4.1.0"},"reference-count":16,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006312","name":"BrightFocus Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006312","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014498","name":"Stanford University Chemistry Engineering & Medicine for Human Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014498","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2020\/07365-8"],"award-info":[{"award-number":["2020\/07365-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/01956-8"],"award-info":[{"award-number":["2022\/01956-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30EY026877"],"award-info":[{"award-number":["P30EY026877"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY024665"],"award-info":[{"award-number":["R01EY024665"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY025225"],"award-info":[{"award-number":["R01EY025225"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY030151"],"award-info":[{"award-number":["R01EY030151"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY031952"],"award-info":[{"award-number":["R01EY031952"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.xpro.2022.101754","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T00:01:04Z","timestamp":1665100864000},"page":"101754","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system"],"prefix":"10.1016","volume":"3","author":[{"given":"Julian","family":"Wolf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds E.","family":"Sabage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Joo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinit B.","family":"Mahajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2022.101754_bib1","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.exer.2012.06.009","article-title":"Human ocular biometry","volume":"102","author":"Augusteyn","year":"2012","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.xpro.2022.101754_bib2","first-page":"2173","article-title":"Effects of hyaluronan lyase, hyaluronidase, and chondroitin ABC lyase on mammalian vitreous gel","volume":"40","author":"Bishop","year":"1999","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.xpro.2022.101754_bib3","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3109\/02713689608995835","article-title":"Cleavage of structural components of mammalian vitreous by endogenous matrix metalloproteinase-2","volume":"15","author":"Brown","year":"1996","journal-title":"Curr. Eye Res."},{"key":"10.1016\/j.xpro.2022.101754_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000267137","article-title":"Metal ion catalyzed liquefaction of vitreous by ascorbic acid: role of radicals and radical ions","volume":"24","author":"Chattopadhyay","year":"1992","journal-title":"Ophthalmic Res."},{"key":"10.1016\/j.xpro.2022.101754_bib5","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1038\/eye.2008.21","article-title":"Adult vitreous structure and postnatal changes","volume":"22","author":"le Goff","year":"2008","journal-title":"Eye"},{"key":"10.1016\/j.xpro.2022.101754_bib6","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ajo.2009.07.036","article-title":"The vitreous gel: more than meets the eye","volume":"149","author":"Holekamp","year":"2010","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.xpro.2022.101754_bib7","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ejps.2018.03.023","article-title":"Effects of enzymatic degradation on dynamic mechanical properties of the vitreous and intravitreal nanoparticle mobility","volume":"118","author":"Huang","year":"2018","journal-title":"Eur. J. Pharm. Sci."},{"key":"10.1016\/j.xpro.2022.101754_bib8","doi-asserted-by":"crossref","first-page":"633","DOI":"10.5301\/ejo.5000802","article-title":"Decreased hyaluronan concentration during primary rhegmatogenous retinal detachment","volume":"26","author":"Kaprinis","year":"2016","journal-title":"Eur. J. Ophthalmol."},{"key":"10.1016\/j.xpro.2022.101754_bib9","first-page":"306","article-title":"Liquefaction for cataract extraction","volume":"9","author":"Labiris","year":"2016","journal-title":"Int. J. Ophthalmol."},{"key":"10.1016\/j.xpro.2022.101754_bib10","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.cvfa.2010.09.002","article-title":"Porcine ophthalmology","volume":"26","author":"Middleton","year":"2010","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.xpro.2022.101754_bib11","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1002\/bmc.336","article-title":"Variations in content and structure of glycosaminoglycans of the vitreous gel from different mammalian species","volume":"18","author":"Noulas","year":"2004","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.xpro.2022.101754_bib12","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/BF02160337","article-title":"Age-related changes in human vitreous structure","volume":"225","author":"Sebag","year":"1987","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.xpro.2022.101754_bib13","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1038\/eye.1987.45","article-title":"Ageing of the vitreous","volume":"1","author":"Sebag","year":"1987","journal-title":"Eye"},{"key":"10.1016\/j.xpro.2022.101754_bib14","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/eye.1992.119","article-title":"Anatomy and pathology of the vitreo-retinal interface","volume":"6","author":"Sebag","year":"1992","journal-title":"Eye"},{"key":"10.1016\/j.xpro.2022.101754_bib15","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.carbpol.2019.03.032","article-title":"Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate","volume":"215","author":"Shen","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.xpro.2022.101754_bib16","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1080\/08820538.2017.1353832","article-title":"Postoperative complications of pars plana vitrectomy for diabetic retinal disease.","volume":"33","author":"Yau","year":"2018","journal-title":"Semin. Ophthalmol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166722006347?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166722006347?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:43:57Z","timestamp":1790927037000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166722006347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["S2666166722006347"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2022.101754","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2022.101754","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"name":"copyright","label":"Copyright"}],"article-number":"101754"}}