{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T15:22:21Z","timestamp":1790954541490,"version":"4.1.0"},"reference-count":13,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T00:00:00Z","timestamp":1668384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["FKZ8009701709"],"award-info":[{"award-number":["FKZ8009701709"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["FKZ8009801921"],"award-info":[{"award-number":["FKZ8009801921"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.xpro.2022.101902","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:44Z","timestamp":1668556844000},"page":"101902","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"title":["Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens"],"prefix":"10.1016","volume":"3","author":[{"given":"Leonie M.","family":"Weskamm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Dahlke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marylyn M.","family":"Addo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2022.101902_bib1","doi-asserted-by":"crossref","first-page":"100685","DOI":"10.1016\/j.xcrm.2022.100685","article-title":"Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine","volume":"3","author":"Weskamm","year":"2022","journal-title":"Cell Rep. Med."},{"key":"10.1016\/j.xpro.2022.101902_bib2","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/s41577-019-0244-2","article-title":"B cell memory: building two walls of protection against pathogens","volume":"20","author":"Akkaya","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.xpro.2022.101902_bib3","doi-asserted-by":"crossref","first-page":"4998","DOI":"10.1182\/blood-2009-03-211052","article-title":"Appearance of peripheral blood plasma cells and memory B cells in a primary and secondary immune response in humans","volume":"114","author":"Blanchard-Rohner","year":"2009","journal-title":"Blood"},{"key":"10.1016\/j.xpro.2022.101902_bib4","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.3389\/fimmu.2019.01787","article-title":"Remembrance of things past: long-term B cell memory after infection and vaccination","volume":"10","author":"Palm","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.xpro.2022.101902_bib5","doi-asserted-by":"crossref","first-page":"2458","DOI":"10.3389\/fimmu.2019.02458","article-title":"Challenges and opportunities for consistent classification of human B cell and plasma cell populations","volume":"10","author":"Sanz","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.xpro.2022.101902_bib6","series-title":"Plotkin's Vaccines","first-page":"16","article-title":"2 - vaccine immunology","author":"Siegrist","year":"2018"},{"key":"10.1016\/j.xpro.2022.101902_bib7","doi-asserted-by":"crossref","first-page":"eabf4063","DOI":"10.1126\/science.abf4063","article-title":"Immunological memory to SARS-CoV-2 assessed for up to 8\u00a0months after infection","volume":"371","author":"Dan","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.xpro.2022.101902_bib8","doi-asserted-by":"crossref","first-page":"eabi6950","DOI":"10.1126\/sciimmunol.abi6950","article-title":"Distinct antibody and memory B cell responses in SARS-CoV-2 na\u00efve and recovered individuals following mRNA vaccination","volume":"6","author":"Goel","year":"2021","journal-title":"Sci. Immunol."},{"key":"10.1016\/j.xpro.2022.101902_bib9","article-title":"Humoral and cellular immune memory to four COVID-19 vaccines","author":"Zhang","year":"2022","journal-title":"bioRxiv"},{"key":"10.1016\/j.xpro.2022.101902_bib10","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.1016\/j.cell.2022.09.032","article-title":"Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses","volume":"185","author":"Buckner","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.xpro.2022.101902_bib11","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.3389\/fimmu.2019.01452","article-title":"Functional enrichment and analysis of antigen-specific memory B cell antibody repertoires in PBMCs","volume":"10","author":"Waltari","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.xpro.2022.101902_bib12","doi-asserted-by":"crossref","first-page":"e0213832","DOI":"10.1371\/journal.pone.0213832","article-title":"Isolation of B-cells using Miltenyi MACS bead isolation kits","volume":"14","author":"Moore","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2022.101902_bib13","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.3389\/fimmu.2019.01694","article-title":"Techniques to study antigen-specific B cell responses","volume":"10","author":"Boonyaratanakornkit","year":"2019","journal-title":"Front. Immunol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166722007821?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166722007821?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:48:54Z","timestamp":1790927334000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166722007821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["S2666166722007821"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2022.101902","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2022.101902","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.xcrm.2022.100685","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"101902"}}