{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T04:08:23Z","timestamp":1745035703873,"version":"3.40.4"},"reference-count":3,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA250025","R50CA265332","R01CA275846","R01CA246765","R01CA265892"],"award-info":[{"award-number":["R01CA250025","R50CA265332","R01CA275846","R01CA246765","R01CA265892"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2024,9]]},"DOI":"10.1016\/j.xpro.2024.103303","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T14:09:28Z","timestamp":1726150168000},"page":"103303","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence"],"prefix":"10.1016","volume":"5","author":[{"given":"Anxo","family":"Martinez-Ordo\u00f1ez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroto","family":"Kinoshita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania","family":"Cid-Diaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angeles","family":"Duran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Osrodek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria T.","family":"Diaz-Meco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9442-3834","authenticated-orcid":false,"given":"Jorge","family":"Moscat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2024.103303_bib1","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1016\/j.devcel.2024.05.001","article-title":"Epithelial aPKC deficiency leads to stem cell loss preceding metaplasia in colorectal cancer initiation","volume":"59","author":"Kinoshita","year":"2024","journal-title":"Dev. Cell"},{"key":"10.1016\/j.xpro.2024.103303_bib2","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.ccell.2022.11.016","article-title":"Hyaluronan driven by epithelial aPKC deficiency remodels the microenvironment and creates a vulnerability in mesenchymal colorectal cancer","volume":"41","author":"Martinez-Ordonez","year":"2023","journal-title":"Cancer Cell"},{"key":"10.1016\/j.xpro.2024.103303_bib3","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1016\/j.immuni.2018.09.013","article-title":"Simultaneous Loss of Both Atypical Protein Kinase C Genes in the Intestinal Epithelium Drives Serrated Intestinal Cancer by Impairing Immunosurveillance","volume":"49","author":"Nakanishi","year":"2018","journal-title":"Immunity"}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724004684?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724004684?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T17:23:48Z","timestamp":1744997028000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166724004684"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":3,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["S2666166724004684"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103303","relation":{},"ISSN":["2666-1667"],"issn-type":[{"type":"print","value":"2666-1667"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103303","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.devcel.2024.05.001","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103303"}}