{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:53:46Z","timestamp":1791986026610,"version":"4.4.0"},"reference-count":13,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007219","name":"Shanghai Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["22ZR1469700"],"award-info":[{"award-number":["22ZR1469700"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2019YFA0801603"],"award-info":[{"award-number":["2019YFA0801603"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31701265"],"award-info":[{"award-number":["31701265"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Chinese Academy of Sciences Youth Innovation Promotion Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.xpro.2024.103490","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T07:42:15Z","timestamp":1734075735000},"page":"103490","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Protocol for generating a pericyte reporter zebrafish\u00a0line\u00a0Ki(pdgfrb-P2A-GAL4-VP16) using a\u00a0CRISPR-Cas9-mediated knockin technique"],"prefix":"10.1016","volume":"6","author":[{"given":"Huaxing","family":"Zi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiulin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2024.103490_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.113652","article-title":"Piezo1-dependent regulation of pericyte proliferation by blood flow during brain vascular development","volume":"43","author":"Zi","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.xpro.2024.103490_bib2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1242\/dev.096107","article-title":"Notch3 establishes brain vascular integrity by regulating pericyte number","volume":"141","author":"Wang","year":"2014","journal-title":"Development"},{"key":"10.1016\/j.xpro.2024.103490_bib3","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1038\/cr.2015.43","article-title":"Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR\/Cas9 system","volume":"25","author":"Li","year":"2015","journal-title":"Cell Res."},{"key":"10.1016\/j.xpro.2024.103490_bib4","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.devcel.2019.10.004","article-title":"Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish","volume":"51","author":"Hoshijima","year":"2019","journal-title":"Dev. Cell"},{"key":"10.1016\/j.xpro.2024.103490_bib5","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1038\/s41467-022-31034-8","article-title":"SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish","volume":"13","author":"Liang","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.xpro.2024.103490_bib6","doi-asserted-by":"crossref","first-page":"3757","DOI":"10.1242\/dev.126.17.3757","article-title":"nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate","volume":"126","author":"Lister","year":"1999","journal-title":"Development"},{"key":"10.1016\/j.xpro.2024.103490_bib7","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1073\/pnas.0704963105","article-title":"Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish","volume":"105","author":"Asakawa","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2024.103490_bib8","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ydbio.2011.01.002","article-title":"Transgenerational analysis of transcriptional silencing in zebrafish","volume":"352","author":"Akitake","year":"2011","journal-title":"Dev. Biol."},{"key":"10.1016\/j.xpro.2024.103490_bib9","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.ydbio.2009.02.031","article-title":"A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf\/Plcg1 signaling during artery development","volume":"329","author":"Covassin","year":"2009","journal-title":"Dev. Biol."},{"key":"10.1016\/j.xpro.2024.103490_bib11","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1006\/dbio.2000.9995","article-title":"The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development","volume":"230","author":"Isogai","year":"2001","journal-title":"Dev. Biol."},{"key":"10.1016\/j.xpro.2024.103490_bib10","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nmeth1033","article-title":"Targeting neural circuitry in zebrafish using GAL4 enhancer trapping","volume":"4","author":"Scott","year":"2007","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2024.103490_bib12","doi-asserted-by":"crossref","DOI":"10.1242\/dev.200189","article-title":"Integrated molecular analysis identifies a conserved pericyte gene signature in zebrafish","volume":"148","author":"Shih","year":"2021","journal-title":"Development"},{"key":"10.1016\/j.xpro.2024.103490_bib13","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1016\/j.devcel.2023.07.022","article-title":"Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish","volume":"58","author":"Shin","year":"2023","journal-title":"Dev. Cell"}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724006555?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724006555?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:40:30Z","timestamp":1776876030000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166724006555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["S2666166724006555"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103490","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for generating a pericyte reporter zebrafish\u00a0line\u00a0Ki(pdgfrb-P2A-GAL4-VP16) using a\u00a0CRISPR-Cas9-mediated knockin technique","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103490","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2023.113652","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103490"}}