{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T08:14:26Z","timestamp":1778919266980,"version":"3.51.4"},"reference-count":21,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["BIG 2022"],"award-info":[{"award-number":["BIG 2022"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/146839\/2020"],"award-info":[{"award-number":["SFRH\/BD\/146839\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/138636\/2018"],"award-info":[{"award-number":["SFRH\/BD\/138636\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIA-BID\/28572\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BID\/28572\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["DL57\/2016"],"award-info":[{"award-number":["DL57\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100003146","name":"Morton Cure Paralysis Fund Minnesota","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100003146","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.xpro.2024.103491","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T10:46:46Z","timestamp":1733741206000},"page":"103491","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures"],"prefix":"10.1016","volume":"5","author":[{"given":"Mariana","family":"Rebocho da Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Quit\u00e9ria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalila","family":"Neves-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonor","family":"Sa\u00fade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2024.103491_bib1","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.230103","article-title":"Development and repair of blood vessels in the zebrafish spinal cord","volume":"13","author":"Ribeiro","year":"2023","journal-title":"Open Biol."},{"key":"10.1016\/j.xpro.2024.103491_bib2","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1038\/nn.2928","article-title":"Scale: A chemical approach for fluorescence imaging and reconstruction of transparent mouse brain","volume":"14","author":"Hama","year":"2011","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.xpro.2024.103491_bib3","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1038\/nn.4107","article-title":"ScaleS: An optical clearing palette for biological imaging","volume":"18","author":"Hama","year":"2015","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.xpro.2024.103491_bib4","doi-asserted-by":"crossref","first-page":"5199","DOI":"10.1242\/dev.02087","article-title":"Cellular and molecular analyses of vascular tube and lumen formation in zebrafish","volume":"132","author":"Jin","year":"2005","journal-title":"Development"},{"key":"10.1016\/j.xpro.2024.103491_bib5","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1101\/gad.1629408","article-title":"Foxn4 directly regulates tbx2b expression and atrioventricular canal formation","volume":"22","author":"Chi","year":"2008","journal-title":"Genes Dev."},{"key":"10.1016\/j.xpro.2024.103491_bib6","doi-asserted-by":"crossref","first-page":"3119","DOI":"10.1242\/dev.048785","article-title":"Moesin1 and Ve-cadherin are required in endothelial cells during in\u00a0vivo tubulogenesis","volume":"137","author":"Wang","year":"2010","journal-title":"Development"},{"key":"10.1016\/j.xpro.2024.103491_bib7","doi-asserted-by":"crossref","first-page":"e49","DOI":"10.1182\/blood-2010-10-314120","article-title":"mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish","volume":"117","author":"Ellett","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.xpro.2024.103491_bib8","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2024.103491_bib9","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.stem.2007.11.002","article-title":"Transparent Adult Zebrafish as a Tool for In\u00a0Vivo Transplantation Analysis","volume":"2","author":"White","year":"2008","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.xpro.2024.103491_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2022.101759","article-title":"Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords","volume":"3","author":"Yu","year":"2022","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2024.103491_bib11","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.1002\/dvdy.22438","article-title":"Cellular response after crush injury in adult zebrafish spinal cord","volume":"239","author":"Hui","year":"2010","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.xpro.2024.103491_bib12","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.cell.2014.10.010","article-title":"IDISCO: A simple, rapid method to immunolabel large tissue samples for volume imaging","volume":"159","author":"Renier","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.xpro.2024.103491_bib13","doi-asserted-by":"crossref","DOI":"10.15252\/msb.20209807","article-title":"A guidebook for DISCO tissue clearing","volume":"17","author":"Molbay","year":"2021","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.xpro.2024.103491_bib14","first-page":"489","article-title":"Tissue Clearing Technique: Recent Progress and Biomedical Applications","volume":"238","author":"Tian","year":"2021","journal-title":"Blackwell Publishing Ltd"},{"key":"10.1016\/j.xpro.2024.103491_bib15","doi-asserted-by":"crossref","first-page":"e443","DOI":"10.1002\/cpz1.443","article-title":"Analytical Approaches for the Segmentation of the Zebrafish Brain Vasculature","volume":"2","author":"Kugler","year":"2022","journal-title":"Curr. Protoc."},{"key":"10.1016\/j.xpro.2024.103491_bib16","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.devcel.2020.12.009","article-title":"A switch in pdgfrb+ cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord","volume":"56","author":"Tsata","year":"2021","journal-title":"Dev. Cell"},{"key":"10.1016\/j.xpro.2024.103491_bib17","doi-asserted-by":"crossref","DOI":"10.1242\/dev.201162","article-title":"Progenitor-derived glia are required for spinal cord regeneration in zebrafish","volume":"150","author":"Zhou","year":"2023","journal-title":"Development"},{"key":"10.1016\/j.xpro.2024.103491_bib18","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.jgg.2012.07.010","article-title":"A Surgery Protocol for Adult Zebrafish Spinal Cord Injury","volume":"39","author":"Fang","year":"2012","journal-title":"J. Genet. Genomics"},{"key":"10.1016\/j.xpro.2024.103491_bib19","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.3390\/ijms23031426","article-title":"Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence","volume":"23","author":"Pi\u00f1a","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.xpro.2024.103491_bib20","first-page":"505","article-title":"A novel protocol of whole mount electro-immunofluorescence staining","volume":"15","author":"Liu","year":"2009","journal-title":"Mol. Vis."},{"key":"10.1016\/j.xpro.2024.103491_bib21","doi-asserted-by":"crossref","DOI":"10.1177\/17448069221080305","article-title":"Sudan Black B treatment uncovers the distribution of angiotensin-converting enzyme2 in nociceptors","volume":"18","author":"Su","year":"2022","journal-title":"Mol. Pain"}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724006567?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724006567?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:54:03Z","timestamp":1778918043000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166724006567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["S2666166724006567"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103491","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103491","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103491"}}