{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T19:56:57Z","timestamp":1792871817922,"version":"4.5.0"},"reference-count":22,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006313","name":"Childhood Cancer Foundation","doi-asserted-by":"publisher","award":["FT2023-0023"],"award-info":[{"award-number":["FT2023-0023"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006313","name":"Childhood Cancer Foundation","doi-asserted-by":"publisher","award":["TJ2022-0016"],"award-info":[{"award-number":["TJ2022-0016"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006313","name":"Childhood Cancer Foundation","doi-asserted-by":"publisher","award":["PR2023-0111"],"award-info":[{"award-number":["PR2023-0111"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007435","name":"\u00c5ke Wiberg Stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007435","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008457","name":"University of Virginia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008457","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Swedish Cancer Society","doi-asserted-by":"publisher","award":["24 3787 Pj"],"award-info":[{"award-number":["24 3787 Pj"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Swedish Cancer Society","doi-asserted-by":"publisher","award":["21 0371 FE"],"award-info":[{"award-number":["21 0371 FE"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.xpro.2024.103505","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T17:18:19Z","timestamp":1736961499000},"page":"103505","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Protocol for in\u00a0vivo immune cell analysis in subcutaneous murine tumor models using advanced\u00a0flow cytometry"],"prefix":"10.1016","volume":"6","author":[{"given":"Katrine","family":"Ingelshed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marit M.","family":"Melssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1509-2142","authenticated-orcid":false,"given":"Diana","family":"Spiegelberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2024.103505_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2024.109862","article-title":"MDM2\/MDMX inhibition by Sulanemadlin synergizes with anti-Programmed Death 1 immunotherapy in wild-type p53 tumors","volume":"27","author":"Ingelshed","year":"2024","journal-title":"iScience"},{"key":"10.1016\/j.xpro.2024.103505_bib2","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1016\/j.cell.2023.03.006","article-title":"Immune checkpoint therapy\u2014current perspectives and future directions","volume":"186","author":"Sharma","year":"2023","journal-title":"Cell"},{"key":"10.1016\/j.xpro.2024.103505_bib3","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1212476","article-title":"Cancer immunotherapies: advances and bottlenecks","volume":"14","author":"Rui","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.xpro.2024.103505_bib4","article-title":"Immunotherapy: Reshape the Tumor Immune Microenvironment","volume":"13","author":"Lv","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.xpro.2024.103505_bib5","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.ccell.2023.02.016","article-title":"The evolving tumor microenvironment: From cancer initiation to metastatic outgrowth","volume":"41","author":"de Visser","year":"2023","journal-title":"Cancer Cell"},{"key":"10.1016\/j.xpro.2024.103505_bib6","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/cyto.a.23690","article-title":"Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry","volume":"95","author":"Reichard","year":"2019","journal-title":"Cytometry A."},{"key":"10.1016\/j.xpro.2024.103505_bib7","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.3389\/fimmu.2019.01078","article-title":"Cell Adhesion Molecules and Their Roles and Regulation in the Immune and Tumor Microenvironment","volume":"10","author":"Harjunp\u00e4\u00e4","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.xpro.2024.103505_bib8","doi-asserted-by":"crossref","DOI":"10.3389\/fonc.2022.1058894","article-title":"NK cells reduce anergic T\u00a0cell development in early-stage tumors by promoting myeloid cell maturation","volume":"12","author":"Lindsay","year":"2022","journal-title":"Front. Oncol."},{"key":"10.1016\/j.xpro.2024.103505_bib9","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1158\/2326-6066.CIR-17-0190","article-title":"Differential Expression of Homing Receptor Ligands on Tumor-Associated Vasculature that Control CD8 Effector T-cell Entry","volume":"5","author":"Woods","year":"2017","journal-title":"Cancer Immunol. Res."},{"key":"10.1016\/j.xpro.2024.103505_bib10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.smim.2017.12.004","article-title":"Neutrophils and PMN-MDSC: Their biological role and interaction with stromal cells","volume":"35","author":"Zhou","year":"2018","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.xpro.2024.103505_bib11","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20201803","article-title":"Analysis of classical neutrophils and polymorphonuclear myeloid-derived suppressor cells in cancer patients and tumor-bearing mice","volume":"218","author":"Veglia","year":"2021","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.xpro.2024.103505_bib12","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/978-1-4939-7837-3_12","article-title":"Polarizing Macrophages In\u00a0Vitro","volume":"1784","author":"Huang","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.xpro.2024.103505_bib13","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-92784-x","article-title":"A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes","volume":"11","author":"Nordlohne","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2024.103505_bib14","article-title":"Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity","volume":"3","author":"Mirlekar","year":"2022","journal-title":"Cell Rep. Med."},{"key":"10.1016\/j.xpro.2024.103505_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.113108","article-title":"STING signaling promotes NK cell antitumor immunity and maintains a reservoir of TCF-1+ NK cells","volume":"42","author":"Lu","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.xpro.2024.103505_bib16","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1016\/j.ccell.2023.04.010","article-title":"Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma","volume":"41","author":"Ramachandran","year":"2023","journal-title":"Cancer Cell"},{"key":"10.1016\/j.xpro.2024.103505_bib17","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.immuni.2014.10.004","article-title":"T follicular helper cell differentiation, function, and roles in disease","volume":"41","author":"Crotty","year":"2014","journal-title":"Immunity"},{"key":"10.1016\/j.xpro.2024.103505_bib18","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1146\/annurev-immunol-031210-101400","article-title":"Follicular Helper CD4\u00a0T Cells (TFH)","volume":"29","author":"Crotty","year":"2011","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.xpro.2024.103505_bib19","series-title":"Macrophages in the Human Body","first-page":"67","article-title":"Chapter 4 - Spleen, lymph nodes, and lymphoid tissues\u2014Inflammatory system","author":"Lima-Junior","year":"2022"},{"key":"10.1016\/j.xpro.2024.103505_bib20","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1159\/000337007","article-title":"Lymph node macrophages","volume":"4","author":"Gray","year":"2012","journal-title":"J.\u00a0Innate Immun."},{"key":"10.1016\/j.xpro.2024.103505_bib21","doi-asserted-by":"crossref","first-page":"12295","DOI":"10.1073\/pnas.1921673117","article-title":"Marginal zone SIGN-R1(+) macrophages are essential for the maturation of germinal center B cells in the spleen","volume":"117","author":"Pirgova","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2024.103505_bib22","doi-asserted-by":"crossref","first-page":"480","DOI":"10.3389\/fimmu.2015.00480","article-title":"Splenic Macrophage Subsets and Their Function during Blood-Borne Infections","volume":"6","author":"Borges da Silva","year":"2015","journal-title":"Front. Immunol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724006701?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166724006701?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:37:14Z","timestamp":1776875834000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166724006701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["S2666166724006701"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103505","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for in\u00a0vivo immune cell analysis in subcutaneous murine tumor models using advanced\u00a0flow cytometry","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2024.103505","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2024.109862","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103505"}}