{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T17:40:50Z","timestamp":1776879650340,"version":"3.51.2"},"reference-count":10,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FDN-154288"],"award-info":[{"award-number":["FDN-154288"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008762","name":"Genome Canada","doi-asserted-by":"publisher","award":["R01AI127375"],"award-info":[{"award-number":["R01AI127375"]}],"id":[{"id":"10.13039\/100008762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007631","name":"Canadian Institute for Advanced Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007631","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.xpro.2025.103645","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T16:41:52Z","timestamp":1740069712000},"page":"103645","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protocol to identify genes important for Candida albicans fitness in diverse environmental conditions using pooled bar-seq screening approach"],"prefix":"10.1016","volume":"6","author":[{"given":"Emily H.","family":"Xiong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Robbins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chad L.","family":"Myers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-0110","authenticated-orcid":false,"given":"Leah E.","family":"Cowen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.103645_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114601","article-title":"Functional genomic analysis of genes important for Candida albicans fitness in diverse environmental conditions","volume":"43","author":"Xiong","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.xpro.2025.103645_bib2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1046\/j.1365-2958.2003.03697.x","article-title":"Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery","volume":"50","author":"Roemer","year":"2003","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.xpro.2025.103645_bib3","doi-asserted-by":"crossref","first-page":"6497","DOI":"10.1038\/s41467-021-26850-3","article-title":"Leveraging machine learning essentiality predictions and chemogenomic interactions to identify antifungal targets","volume":"12","author":"Fu","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.xpro.2025.103645_bib4","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2025.103645_bib5","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.xpro.2025.103645_bib6","doi-asserted-by":"crossref","first-page":"D592","DOI":"10.1093\/nar\/gkw924","article-title":"The Candida genome database (CGD): Incorporation of assembly 22, systematic identifiers and visualization of high throughput sequencing data","volume":"45","author":"Skrzypek","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.xpro.2025.103645_bib7","series-title":"RStudio: Integrated Development Environment for R","year":"2020"},{"key":"10.1016\/j.xpro.2025.103645_bib8","doi-asserted-by":"crossref","DOI":"10.15252\/msb.202010013","article-title":"A method for benchmarking genetic screens reveals a predominant mitochondrial bias","volume":"17","author":"Rahman","year":"2021","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.xpro.2025.103645_bib9","first-page":"127","article-title":"Nuclear DNA content and genome size of trout and human","volume":"51","author":"Dolezel","year":"2003","journal-title":"Cytometry A."},{"key":"10.1016\/j.xpro.2025.103645_bib10","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.cels.2023.04.003","article-title":"Reproducibility metrics for context-specific CRISPR screens","volume":"14","author":"Billmann","year":"2023","journal-title":"Cell Syst."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725000516?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725000516?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:45:08Z","timestamp":1776876308000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725000516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["S2666166725000516"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.103645","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol to identify genes important for Candida albicans fitness in diverse environmental conditions using pooled bar-seq screening approach","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.103645","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103645"}}