{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:19:29Z","timestamp":1788927569176,"version":"build-2803163510"},"reference-count":7,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,6]],"date-time":"2025-04-06T00:00:00Z","timestamp":1743897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R35 CA231620"],"award-info":[{"award-number":["R35 CA231620"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["DKH 70115382"],"award-info":[{"award-number":["DKH 70115382"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KA 4830\/1-1"],"award-info":[{"award-number":["KA 4830\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001767","name":"National Foundation for Cancer Research","doi-asserted-by":"publisher","award":["R35 CA231620"],"award-info":[{"award-number":["R35 CA231620"]}],"id":[{"id":"10.13039\/100001767","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01EO2104"],"award-info":[{"award-number":["01EO2104"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008349","name":"Universit\u00e4t Duisburg-Essen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010571","name":"Bundesministerium f\u00fcr Bildung, Wissenschaft, Forschung und Technologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012524","name":"American Lebanese Syrian Associated Charities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012524","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.xpro.2025.103793","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T09:34:11Z","timestamp":1745400851000},"page":"103793","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protocol for the establishment of murine orthotopic lung tumors and the assessment of contralateral pulmonal metastasis by flow cytometry"],"prefix":"10.1016","volume":"6","author":[{"given":"Gregor","family":"Zaun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Webend\u00f6rfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abubakar","family":"Wani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas R.","family":"Green","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0090-4080","authenticated-orcid":false,"given":"Halime","family":"Kalkavan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.103793_bib1","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1016\/j.cell.2022.07.025","article-title":"Sublethal cytochrome c release generates drug-tolerant persister cells","volume":"185","author":"Kalkavan","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.xpro.2025.103793_bib2","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1038\/nprot.2007.296","article-title":"Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester","volume":"2","author":"Quah","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.xpro.2025.103793_bib3","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1097\/JTO.0b013e3182725ff9","article-title":"Evaluation of Novel Orthotopic Nude Mouse Models for Human Small-Cell Lung Cancer","volume":"8","author":"Isobe","year":"2013","journal-title":"J. Thorac. Oncol."},{"key":"10.1016\/j.xpro.2025.103793_bib4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/ng.3728","article-title":"Inactivation of Capicua drives cancer metastasis","volume":"49","author":"Okimoto","year":"2017","journal-title":"Nat. Genet."},{"key":"10.1016\/j.xpro.2025.103793_bib5","doi-asserted-by":"crossref","first-page":"234","DOI":"10.4143\/crt.2006.38.4.234","article-title":"Mouse Orthotopic Lung Cancer Model Induced by PC14PE6","volume":"38","author":"Cui","year":"2006","journal-title":"Cancer Res. Treat."},{"key":"10.1016\/j.xpro.2025.103793_bib6","first-page":"141","article-title":"Establishment of an orthotopic lung cancer model in nude mice and itsevaluation by spiral CT","volume":"4","author":"Liu","year":"2012","journal-title":"J. Thorac. Dis."},{"key":"10.1016\/j.xpro.2025.103793_bib7","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1002\/eji.202170126","article-title":"Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)","volume":"51","author":"Cossarizza","year":"2021","journal-title":"Eur. J. Immunol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725001996?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725001996?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:54:32Z","timestamp":1778918072000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725001996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":7,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S2666166725001996"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.103793","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for the establishment of murine orthotopic lung tumors and the assessment of contralateral pulmonal metastasis by flow cytometry","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.103793","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.cell.2022.07.025","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103793"}}