{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T20:56:35Z","timestamp":1781816195541,"version":"3.54.5"},"reference-count":8,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BA 3824\/3-2"],"award-info":[{"award-number":["BA 3824\/3-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RO 5599\/1-1"],"award-info":[{"award-number":["RO 5599\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RO 5599\/1-2"],"award-info":[{"award-number":["RO 5599\/1-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KFO325 BA 3824\/3-1"],"award-info":[{"award-number":["KFO325 BA 3824\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.xpro.2025.103981","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T11:43:40Z","timestamp":1753530220000},"page":"103981","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for analyzing T cell and macrophage motility in a mouse pancreatic cancer spheroid model using light-sheet microscopy"],"prefix":"10.1016","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5509-315X","authenticated-orcid":false,"given":"Vanessa","family":"Zimmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedikt","family":"Slusny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Roth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.103981_bib1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3390\/cancers17010051","article-title":"Optimized Spheroid Model of Pancreatic Cancer Demonstrates Influence of Macrophage-T Cell Interaction for Intratumoral T Cell Motility","volume":"17","author":"Slusny","year":"2024","journal-title":"Cancers"},{"key":"10.1016\/j.xpro.2025.103981_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/cells12030456","article-title":"IL18 Receptor Signaling Inhibits Intratumoral CD8+ T-Cell Migration in a Murine Pancreatic Cancer Model","volume":"12","author":"Nasiri","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.xpro.2025.103981_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2020.100246","article-title":"Preparation and culture of bone marrow-derived macrophages from mice for functional analysis","volume":"2","author":"Toda","year":"2021","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2025.103981_bib4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1158\/2326-6066.CIR-22-0398","article-title":"IL18 Receptor Signaling Regulates Tumor-Reactive CD8+ T-cell Exhaustion via Activation of the IL2\/STAT5\/mTOR Pathway in a Pancreatic Cancer Model","volume":"11","author":"Lutz","year":"2023","journal-title":"Cancer Immunol. Res."},{"key":"10.1016\/j.xpro.2025.103981_bib5","first-page":"316","article-title":"Hydrogel scaffolds for tissue engineering: Progress and challenges","volume":"2013","author":"El-Sherbiny","year":"2013","journal-title":"Glob. Cardiol. Sci. Pract."},{"key":"10.1016\/j.xpro.2025.103981_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ijms222212200","article-title":"3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages","volume":"22","author":"Habanjar","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.xpro.2025.103981_bib7","doi-asserted-by":"crossref","first-page":"17253","DOI":"10.1073\/pnas.1308887110","article-title":"Modulation of macrophage phenotype by cell shape","volume":"110","author":"McWhorter","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2025.103981_bib8","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/s41578-020-0199-8","article-title":"Synthetic alternatives to Matrigel","volume":"5","author":"Aisenbrey","year":"2020","journal-title":"Nat. Rev. Mater."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725003879?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725003879?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T20:21:46Z","timestamp":1781814106000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725003879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":8,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S2666166725003879"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.103981","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for analyzing T cell and macrophage motility in a mouse pancreatic cancer spheroid model using light-sheet microscopy","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.103981","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"103981"}}