{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T21:23:46Z","timestamp":1783027426803,"version":"3.54.6"},"reference-count":21,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["PE0000006"],"award-info":[{"award-number":["PE0000006"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["1553"],"award-info":[{"award-number":["1553"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon 2020","doi-asserted-by":"publisher","award":["824164"],"award-info":[{"award-number":["824164"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["CUP D93C22000930002"],"award-info":[{"award-number":["CUP D93C22000930002"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.xpro.2025.104029","type":"journal-article","created":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T16:23:41Z","timestamp":1766852621000},"page":"104029","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol to develop a preclinical severe contusive-compressive SCI mouse model for translational research"],"prefix":"10.1016","volume":"7","author":[{"given":"Sissi","family":"Dolci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia","family":"Di Chio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Busetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Bifari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6627-1263","authenticated-orcid":false,"given":"Ilaria","family":"Decimo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.104029_bib1","doi-asserted-by":"crossref","unstructured":"y Cajal, S.R. (1991). Cajal's degeneration and regeneration of the nervous system (History of Neuroscience).","DOI":"10.1093\/acprof:oso\/9780195065169.003.0011"},{"key":"10.1016\/j.xpro.2025.104029_bib2","first-page":"192","article-title":"Trends in Rates of ASIA Impairment Scale Conversion in Traumatic Complete Spinal Cord Injury","volume":"1","author":"Marino","year":"2020","journal-title":"Neurotrauma Rep."},{"key":"10.1016\/j.xpro.2025.104029_bib3","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1179\/107902611X13186000420242","article-title":"Reference for the 2011 revision of the International Standards for Neurological Classification of Spinal Cord Injury","volume":"34","author":"Kirshblum","year":"2011","journal-title":"J. Spinal Cord Med."},{"key":"10.1016\/j.xpro.2025.104029_bib4","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0140-6736(02)07603-1","article-title":"Spinal-cord injury","volume":"359","author":"McDonald","year":"2002","journal-title":"Lancet"},{"key":"10.1016\/j.xpro.2025.104029_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2022.106149","article-title":"Therapeutic induction of energy metabolism reduces neural tissue damage and increases microglia activation in severe spinal cord injury","volume":"178","author":"Dolci","year":"2022","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.xpro.2025.104029_bib6","doi-asserted-by":"crossref","DOI":"10.1038\/nrdp.2017.18","article-title":"Traumatic spinal cord injury","volume":"3","author":"Ahuja","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"10.1016\/j.xpro.2025.104029_bib7","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/ptj\/80.7.673","article-title":"Vascular events after spinal cord injury: contribution to secondary pathogenesis","volume":"80","author":"Mautes","year":"2000","journal-title":"Phys. Ther."},{"key":"10.1016\/j.xpro.2025.104029_bib8","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1038\/nrn1326","article-title":"Regeneration beyond the glial scar","volume":"5","author":"Silver","year":"2004","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.xpro.2025.104029_bib9","doi-asserted-by":"crossref","first-page":"281","DOI":"10.55782\/ane-2011-1848","article-title":"Secondary injury mechanisms in traumatic spinal cord injury: a nugget of this multiply cascade","volume":"71","author":"Oyinbo","year":"2011","journal-title":"Acta Neurobiol. Exp."},{"key":"10.1016\/j.xpro.2025.104029_bib10","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1002\/glia.22774","article-title":"Macrophages in spinal cord injury: phenotypic and functional change from exposure to myelin debris","volume":"63","author":"Wang","year":"2015","journal-title":"Glia"},{"key":"10.1016\/j.xpro.2025.104029_bib11","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/s41593-018-0324-9","article-title":"Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury","volume":"22","author":"Zhou","year":"2019","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.xpro.2025.104029_bib12","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.bbi.2024.04.013","article-title":"Targeting foamy macrophages by manipulating ABCA1 expression to facilitate lesion healing in the injured spinal cord","volume":"119","author":"Wang","year":"2024","journal-title":"Brain Behav. Immun."},{"key":"10.1016\/j.xpro.2025.104029_bib13","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1089\/neu.2006.23.635","article-title":"Basso Mouse Scale for locomotion detects differences in recovery after spinal cord injury in five common mouse strains","volume":"23","author":"Basso","year":"2006","journal-title":"J. Neurotrauma"},{"key":"10.1016\/j.xpro.2025.104029_bib14","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1089\/neu.2009.1182","article-title":"Sensory stimulation prior to spinal cord injury induces post-injury dysesthesia in mice","volume":"27","author":"Hoschouer","year":"2010","journal-title":"J. Neurotrauma"},{"key":"10.1016\/j.xpro.2025.104029_bib15","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1177\/1535370219854668","article-title":"Effect of M2 macrophage adoptive transfer on transcriptome profile of injured spinal cords in rats","volume":"244","author":"Chen","year":"2019","journal-title":"Exp. Biol. Med."},{"key":"10.1016\/j.xpro.2025.104029_bib16","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1089\/neu.2006.0204","article-title":"Behavioral, histological, and ex vivo magnetic resonance imaging assessment of graded contusion spinal cord injury in mice","volume":"24","author":"Nishi","year":"2007","journal-title":"J. Neurotrauma"},{"key":"10.1016\/j.xpro.2025.104029_bib17","doi-asserted-by":"crossref","first-page":"2342","DOI":"10.1089\/neu.2016.4915","article-title":"Compression Decreases Anatomical and Functional Recovery and Alters Inflammation after Contusive Spinal Cord Injury","volume":"34","author":"Orr","year":"2017","journal-title":"J. Neurotrauma"},{"key":"10.1016\/j.xpro.2025.104029_bib18","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.neuroimage.2012.11.048","article-title":"Vertebral landmarks for the identification of spinal cord segments in the mouse","volume":"68","author":"Harrison","year":"2013","journal-title":"Neuroimage"},{"key":"10.1016\/j.xpro.2025.104029_bib19","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.1089\/neu.2012.2402","article-title":"Persistent polyuria in a rat spinal contusion model","volume":"29","author":"Ward","year":"2012","journal-title":"J. Neurotrauma"},{"key":"10.1016\/j.xpro.2025.104029_bib20","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.bbr.2006.11.042","article-title":"Adaptation of a ladder beam walking task to assess locomotor recovery in mice following spinal cord injury","volume":"177","author":"Cummings","year":"2007","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.xpro.2025.104029_bib21","doi-asserted-by":"crossref","first-page":"703","DOI":"10.3389\/fphar.2017.00703","article-title":"High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy","volume":"8","author":"Dolci","year":"2017","journal-title":"Front. Pharmacol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725004356?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725004356?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T20:55:19Z","timestamp":1783025719000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725004356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["S2666166725004356"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104029","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol to develop a preclinical severe contusive-compressive SCI mouse model for translational research","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.immuni.2025.12.016","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104029"}}