{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T21:43:29Z","timestamp":1773783809109,"version":"3.50.1"},"reference-count":19,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008967","name":"University of Marburg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008967","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.xpro.2025.104205","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T20:05:08Z","timestamp":1763669108000},"page":"104205","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for a mouse tubuloid model of myoglobinuric acute kidney injury"],"prefix":"10.1016","volume":"6","author":[{"given":"Marc","family":"Torres Pereiro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luping","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Worzfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.104205_bib1","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.adk5005","article-title":"Glucocorticoids induce a maladaptive epithelial stress response to aggravate acute kidney injury","volume":"16","author":"Zhou","year":"2024","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.xpro.2025.104205_bib2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/s41587-019-0048-8","article-title":"Tubuloids derived from human adult kidney and urine for personalized disease modeling","volume":"37","author":"Schutgens","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.xpro.2025.104205_bib3","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev-med-050214-013407","article-title":"Acute Kidney Injury","volume":"67","author":"Zuk","year":"2016","journal-title":"Annu. Rev. Med."},{"key":"10.1016\/j.xpro.2025.104205_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2024.103005","article-title":"Protocol for isolating murine kidney tubules and ex vivo cell death assays","volume":"5","author":"Belavgeni","year":"2024","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2025.104205_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2024.103132","article-title":"Protocol for whole-mount immunofluorescence staining of ECM gel-embedded innervated pancreatic organoids","volume":"5","author":"Besikcioglu","year":"2024","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2025.104205_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2021.100386","article-title":"Protocol to profile the bioenergetics of organoids using Seahorse","volume":"2","author":"Ludikhuize","year":"2021","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2025.104205_bib7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.ekir.2022.09.026","article-title":"Molecular Mechanisms of Rhabdomyolysis-Induced Kidney Injury: From Bench to Bedside","volume":"8","author":"Hebert","year":"2023","journal-title":"Kidney Int. Rep."},{"key":"10.1016\/j.xpro.2025.104205_bib8","doi-asserted-by":"crossref","DOI":"10.3390\/cells10092401","article-title":"The Role of HO-1 and Its Crosstalk with Oxidative Stress in Cancer Cell Survival","volume":"10","author":"Chiang","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.xpro.2025.104205_bib9","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1038\/ki.1997.104","article-title":"Myoglobin toxicity in proximal human kidney cells: roles of Fe, Ca2+, H2O2, and terminal mitochondrial electron transport","volume":"51","author":"Zager","year":"1997","journal-title":"Kidney Int."},{"key":"10.1016\/j.xpro.2025.104205_bib10","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1172\/JCI115682","article-title":"Effects of inorganic iron and myoglobin on in vitro proximal tubular lipid peroxidation and cytotoxicity","volume":"89","author":"Zager","year":"1992","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.xpro.2025.104205_bib11","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.21037\/atm-21-3294","article-title":"Vitamin C reduces vancomycin-related nephrotoxicity through the inhibition of oxidative stress, apoptosis, and inflammation in mice","volume":"9","author":"He","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"10.1016\/j.xpro.2025.104205_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2023.149463","article-title":"Prophylactic vitamin C supplementation regulates DNA demethylation to protect against cisplatin-induced acute kidney injury in mice","volume":"695","author":"Yu","year":"2024","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.xpro.2025.104205_bib13","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1159\/000443447","article-title":"Evaluation of Vitamin C Supplementation on Kidney Function and Vascular Reactivity Following Renal Ischemic Injury in Mice","volume":"41","author":"Zhu","year":"2016","journal-title":"Kidney Blood Press. Res."},{"key":"10.1016\/j.xpro.2025.104205_bib14","doi-asserted-by":"crossref","DOI":"10.3390\/nu9070718","article-title":"Protective Role for Antioxidants in Acute Kidney Disease","volume":"9","author":"Dennis","year":"2017","journal-title":"Nutrients"},{"key":"10.1016\/j.xpro.2025.104205_bib15","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1080\/08860220802212965","article-title":"Experimental myoglobinuric acute renal failure: the effect of vitamin C","volume":"30","author":"Ustundag","year":"2008","journal-title":"Ren. Fail."},{"key":"10.1016\/j.xpro.2025.104205_bib16","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1016\/j.freeradbiomed.2012.02.011","article-title":"Cosupplementation with a synthetic, lipid-soluble polyphenol and vitamin C inhibits oxidative damage and improves vascular function yet does not inhibit acute renal injury in an animal model of rhabdomyolysis","volume":"52","author":"Groebler","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.xpro.2025.104205_bib17","first-page":"756","article-title":"Acute Kidney Injury Following High-Dose Vitamin C Treatment","volume":"116","author":"Andres","year":"2019","journal-title":"Dtsch. Arztebl. Int."},{"key":"10.1016\/j.xpro.2025.104205_bib18","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1016\/j.ekir.2020.07.008","article-title":"Oxalate Nephropathy Caused by Excessive Vitamin C Administration in 2 Patients With COVID-19","volume":"5","author":"Fontana","year":"2020","journal-title":"Kidney Int. Rep."},{"key":"10.1016\/j.xpro.2025.104205_bib19","doi-asserted-by":"crossref","first-page":"F788","DOI":"10.1152\/ajprenal.00184.2024","article-title":"Transition from acute kidney injury to chronic kidney disease: mechanisms, models, and biomarkers","volume":"327","author":"Zhang","year":"2024","journal-title":"Am. J. Physiol. Renal. Physiol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725006112?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725006112?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T18:03:59Z","timestamp":1773770639000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725006112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S2666166725006112"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104205","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for a mouse tubuloid model of myoglobinuric acute kidney injury","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104205","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104205"}}