{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T21:49:06Z","timestamp":1773784146372,"version":"3.50.1"},"reference-count":36,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81750110551"],"award-info":[{"award-number":["81750110551"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.xpro.2025.104210","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T10:51:20Z","timestamp":1763981480000},"page":"104210","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for developing a mouse model of gastric cancer following Helicobacter pylori eradication and chronic ethanol feeding"],"prefix":"10.1016","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5802-5855","authenticated-orcid":false,"given":"Faisal","family":"Aziz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4174-4028","authenticated-orcid":false,"given":"Zigang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.104210_bib1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1093\/carcin\/bgab114","article-title":"Gastric tumorigenesis induced by combining Helicobacter pylori infection and chronic alcohol through IL-10 inhibition","volume":"43","author":"Aziz","year":"2022","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.xpro.2025.104210_bib2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.5230\/jgc.2014.14.2.67","article-title":"Mouse models of gastric carcinogenesis","volume":"14","author":"Yu","year":"2014","journal-title":"J. Gastric Cancer"},{"key":"10.1016\/j.xpro.2025.104210_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/cancers12040816","article-title":"Induction and prevention of gastric cancer with combined helicobacter pylori and capsaicin administration and DFMO treatment, respectively","volume":"12","author":"Aziz","year":"2020","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.xpro.2025.104210_bib4","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1007\/s10120-022-01297-7","article-title":"Ubiquitination of ADR\u03b11d\/SerpinA1 complex stimulates hypoxia to induce gastric tumorigenesis with a combination of Helicobacter pylori and chronic stress through IL-1\u03b1","volume":"25","author":"Aziz","year":"2022","journal-title":"Gastric Cancer"},{"key":"10.1016\/j.xpro.2025.104210_bib5","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/nprot.2013.032","article-title":"Mouse model of chronic and binge ethanol feeding (the NIAAA model)","volume":"8","author":"Bertola","year":"2013","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.xpro.2025.104210_bib6","unstructured":"Statistics textbooks in the (2007)."},{"key":"10.1016\/j.xpro.2025.104210_bib7","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jneumeth.2014.02.001","article-title":"Burrowing and nest building behavior as indicators of well-being in mice","volume":"234","author":"Jirkof","year":"2014","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.xpro.2025.104210_bib8","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.neubiorev.2020.07.013","article-title":"The development and use of facial grimace scales for pain measurement in animals","volume":"116","author":"Mogil","year":"2020","journal-title":"Neurosci. Biobehav. Rev."},{"key":"10.1016\/j.xpro.2025.104210_bib9","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4103\/0976-500X.72351","article-title":"Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research","volume":"1","author":"Kilkenny","year":"2010","journal-title":"J. Pharmacol. Pharmacother."},{"key":"10.1016\/j.xpro.2025.104210_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0155001","article-title":"Pain and laboratory animals: Publication practices for better data reproducibility and better animal welfare","volume":"11","author":"Carbone","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2025.104210_bib11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/nprot.2017.136","article-title":"Colonoscopy-based colorectal cancer modeling in mice with CRISPR \u2013 Cas9 genome editing and organoid transplantation","volume":"13","author":"Roper","year":"2018","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.xpro.2025.104210_bib12","series-title":"Guide for the Care and Use of Laboratory Animals","year":"2011"},{"key":"10.1016\/j.xpro.2025.104210_bib13","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1111\/j.1530-0277.2009.00954.x","article-title":"Circadian timing of ethanol exposure exerts enduring effects on subsequent ad libitum consumption in C57 mice","volume":"33","author":"Trujillo","year":"2009","journal-title":"Alcohol Clin. Exp. Res."},{"key":"10.1016\/j.xpro.2025.104210_bib14","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/nmeth.1455","article-title":"Coding of facial expressions of pain in the laboratory mouse","volume":"7","author":"Langford","year":"2010","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2025.104210_bib15","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1158\/1055-9965.EPI-10-0362","article-title":"Alcohol and tobacco use in relation to gastric cancer: a prospective study of men in Shanghai, China","volume":"19","author":"Moy","year":"2010","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"10.1016\/j.xpro.2025.104210_bib16","doi-asserted-by":"crossref","unstructured":"Adriaan Bouwknecht, J., and Paylor, R. Behavioral and physiological mouse assays for anxiety: a survey in nine mouse strains.Behavioural brain research, 136(2), pp.489-501.","DOI":"10.1016\/S0166-4328(02)00200-0"},{"key":"10.1016\/j.xpro.2025.104210_bib17","unstructured":"Lyon, F. (2010). IARC Monographs on the Evaluation of Carcinogenic Risks to Humans VOLUME 96 Alcohol Consumption and Ethyl Carbamate."},{"key":"10.1016\/j.xpro.2025.104210_bib18","first-page":"542","article-title":"Alcohol consumption and ethyl carbamate","volume":"96","year":"2010","journal-title":"IARC Monogr. Eval. Carcinog. Risks Hum."},{"key":"10.1016\/j.xpro.2025.104210_bib20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1177\/0023677213475603","article-title":"Assessment of postsurgical distress and pain in laboratory mice by nest complexity scoring","volume":"47","author":"Jirkof","year":"2013","journal-title":"Lab. Anim."},{"key":"10.1016\/j.xpro.2025.104210_bib21","doi-asserted-by":"crossref","DOI":"10.3791\/51012-v","article-title":"Nest Building as an Indicator of Health and Welfare in Laboratory Mice","author":"Gaskill","year":"2013","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.xpro.2025.104210_bib22","unstructured":"The, P., Health, P., Service, I., Register, F., Act, A.W., and Definitions, I.A. (2003). Guidelines for the Assessment and Management of Pain in Rodents and Rabbits. 1\u201324."},{"key":"10.1016\/j.xpro.2025.104210_bib23","article-title":"Gastric cancer\u2014Epidemiology, modifiable and non-modifiable risk factors, challenges and opportunities: An updated review","volume":"41","author":"Mamun","year":"2024","journal-title":"Cancer Treat. Res. Commun."},{"key":"10.1016\/j.xpro.2025.104210_bib24","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.canlet.2015.01.028","article-title":"Early or late antibiotic intervention prevents helicobacter pylori-induced gastric cancer in a mouse model","volume":"359","author":"Zhang","year":"2015","journal-title":"Cancer Lett."},{"key":"10.1016\/j.xpro.2025.104210_bib25","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s11274-013-1448-4","article-title":"Development of an in-house enzyme-linked immunosorbent assay based on surface whole cell antigen for diagnosis of Helicobacter pylori infection in patients with gastroduodenal ulcer disease","volume":"30","author":"Aziz","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.xpro.2025.104210_bib26","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1128\/CMR.00011-10","article-title":"Helicobacter pylori and gastric cancer: Factors that modulate disease risk","volume":"23","author":"Wroblewski","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.xpro.2025.104210_bib27","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1046\/j.1365-2036.2002.01346.x","article-title":"Alteration of histological gastritis after cure of Helicobacter pylori infection","volume":"16","author":"Hojo","year":"2002","journal-title":"Aliment. Pharmacol. Ther."},{"key":"10.1016\/j.xpro.2025.104210_bib28","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1136\/jcp.45.9.796","article-title":"Quantitative assessment of histological changes in chronic gastritis after eradication of Helicobacter pylori","volume":"45","author":"Di Napoli","year":"1992","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.xpro.2025.104210_bib29","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1007\/s00535-010-0347-9","article-title":"The long-term risk of gastric cancer after the successful eradication of Helicobacter pylori","volume":"46","author":"Take","year":"2011","journal-title":"J. Gastroenterol."},{"key":"10.1016\/j.xpro.2025.104210_bib30","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1128\/IAI.01344-13","article-title":"Deficiency of the myeloid differentiation primary response molecule MyD88 leads to an early and rapid development of Helicobacter-induced gastric malignancy","volume":"82","author":"Banerjee","year":"2014","journal-title":"Infect. Immun."},{"key":"10.1016\/j.xpro.2025.104210_bib31","doi-asserted-by":"crossref","DOI":"10.1111\/hel.70057","article-title":"Long-Term Risk of Gastric Cancer After Helicobacter pylori Eradication in Gastric Ulcer Patients: A Nationwide Cohort Study in Korea","volume":"30","author":"Myeong","year":"2025","journal-title":"Helicobacter"},{"key":"10.1016\/j.xpro.2025.104210_bib32","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1007\/s12328-014-0536-9","article-title":"Metachronous early gastric cancer over a period of 13 years after eradication of Helicobacter pylori","volume":"7","author":"Mitsunaga","year":"2014","journal-title":"Clin. J. Gastroenterol."},{"key":"10.1016\/j.xpro.2025.104210_bib33","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.3748\/wjg.v12.i32.5101","article-title":"Eradication of H pylori for the prevention of gastric cancer","volume":"12","author":"Trautmann","year":"2006","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.xpro.2025.104210_bib34","doi-asserted-by":"crossref","DOI":"10.1186\/s12876-025-04034-3","article-title":"Gastric cancer risk after Helicobacter pylori eradication in gastritis and peptic ulcer: a retrospective cohort study in Japan","volume":"25","author":"Sugano","year":"2025","journal-title":"BMC Gastroenterol."},{"key":"10.1016\/j.xpro.2025.104210_bib35","doi-asserted-by":"crossref","DOI":"10.3390\/cancers16183154","article-title":"Clinicopathologic Features of Early Gastric Cancer after Heli-cobacter pylori Eradication in Japanese Patients: Comparative Study between Early (<10 Years) and Late (>10 Years) Onset","volume":"16","author":"Teshima","year":"2024","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.xpro.2025.104210_bib36","article-title":"Overview: How Is Alcohol Metabolized by the Body?","volume":"29","author":"Zakhari","year":"2006","journal-title":"Alcohol Res. Health"},{"key":"10.1016\/j.xpro.2025.104210_bib37","doi-asserted-by":"crossref","DOI":"10.1186\/s12864-020-6754-2","article-title":"On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments","volume":"21","author":"Assefa","year":"2020","journal-title":"BMC Genom."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725006161?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725006161?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T18:06:33Z","timestamp":1773770793000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725006161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S2666166725006161"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104210","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for developing a mouse model of gastric cancer following Helicobacter pylori eradication and chronic ethanol feeding","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104210","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104210"}}