{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T21:22:14Z","timestamp":1783027334111,"version":"3.54.6"},"reference-count":27,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000858","name":"University of Sheffield","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.xpro.2025.104288","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T10:08:36Z","timestamp":1766570916000},"page":"104288","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells"],"prefix":"10.1016","volume":"7","author":[{"given":"Pei-Li","family":"Tseng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark O.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai S.","family":"Erdmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.104288_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2025.112273","article-title":"Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading","volume":"28","author":"Tseng","year":"2025","journal-title":"iScience"},{"key":"10.1016\/j.xpro.2025.104288_bib2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1038\/nrm2597","article-title":"Mechanotransduction gone awry","volume":"10","author":"Jaalouk","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.xpro.2025.104288_bib3","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.3390\/jcm9051423","article-title":"Mechanotransduction in Wound Healing and Fibrosis","volume":"9","author":"Kuehlmann","year":"2020","journal-title":"J. Clin. Med."},{"key":"10.1016\/j.xpro.2025.104288_bib4","doi-asserted-by":"crossref","first-page":"824","DOI":"10.3389\/fphys.2018.00824","article-title":"Cellular Mechanotransduction: From Tension to Function","volume":"9","author":"Martino","year":"2018","journal-title":"Front. Physiol."},{"key":"10.1016\/j.xpro.2025.104288_bib5","first-page":"245","article-title":"Roles of the cytoskeleton, cell adhesion and rho signalling in mechanosensing and mechanotransduction","volume":"161","author":"Ohashi","year":"2017","journal-title":"J. Biochem."},{"key":"10.1016\/j.xpro.2025.104288_bib6","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2018.0229","article-title":"Mechanotransduction: from the cell surface to the nucleus via RhoA","volume":"374","author":"Burridge","year":"2019","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.xpro.2025.104288_bib7","doi-asserted-by":"crossref","first-page":"141","DOI":"10.4161\/sgtp.25131","article-title":"Dynamic functions of RhoA in tumor cell migration and invasion","volume":"4","author":"O'Connor","year":"2013","journal-title":"Small GTPases"},{"key":"10.1016\/j.xpro.2025.104288_bib8","doi-asserted-by":"crossref","first-page":"26743","DOI":"10.1074\/jbc.M111.233700","article-title":"The Interaction between Nesprins and Sun Proteins at the Nuclear Envelope Is Critical for Force Transmission between the Nucleus and Cytoskeleton","volume":"286","author":"Lombardi","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.xpro.2025.104288_bib9","doi-asserted-by":"crossref","first-page":"a039685","DOI":"10.1101\/cshperspect.a039685","article-title":"Mechanical Forces in Nuclear Organization","volume":"14","author":"Miroshnikova","year":"2022","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"10.1016\/j.xpro.2025.104288_bib10","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1016\/j.cell.2017.10.008","article-title":"Force Triggers YAP Nuclear Entry by Regulating Transport across Nuclear Pores","volume":"171","author":"Elosegui-Artola","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.xpro.2025.104288_bib11","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev-bioeng-060418-052139","article-title":"The Driving Force: Nuclear Mechanotransduction in Cellular Function, Fate, and Disease","volume":"21","author":"Maurer","year":"2019","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"10.1016\/j.xpro.2025.104288_bib12","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1073\/pnas.032668799","article-title":"Engineering gene expression and protein synthesis by modulation of nuclear shape","volume":"99","author":"Thomas","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2025.104288_bib13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/nature10137","article-title":"Role of YAP\/TAZ in mechanotransduction","volume":"474","author":"Dupont","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2025.104288_bib14","doi-asserted-by":"crossref","first-page":"6096","DOI":"10.1074\/jbc.M115.708313","article-title":"YAP Nuclear Localization in the Absence of Cell-Cell Contact Is Mediated by a Filamentous Actin-dependent, Myosin II- and Phospho-YAP-independent Pathway during Extracellular Matrix Mechanosensing","volume":"291","author":"Das","year":"2016","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.xpro.2025.104288_bib15","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nbt.4201","article-title":"Efficient proximity labeling in living cells and organisms with TurboID","volume":"36","author":"Branon","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.xpro.2025.104288_bib16","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.3390\/cells9051070","article-title":"Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation","volume":"9","author":"May","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.xpro.2025.104288_bib17","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2025.104288_bib18","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/bs.mcb.2014.10.008","article-title":"Measurement of cell traction forces with ImageJ","volume":"125","author":"Martiel","year":"2015","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.xpro.2025.104288_bib19","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.243873","article-title":"Cofilin is required for polarization of tension in stress fiber networks during migration","volume":"133","author":"Lee","year":"2020","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.xpro.2025.104288_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0007054","article-title":"Resolving the Role of Actoymyosin Contractility in Cell Microrheology","volume":"4","author":"Hale","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2025.104288_bib21","doi-asserted-by":"crossref","first-page":"17693","DOI":"10.1074\/jbc.REV119.007963","article-title":"Control of cellular responses to mechanical cues through YAP\/TAZ regulation","volume":"294","author":"Dasgupta","year":"2019","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.xpro.2025.104288_bib22","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1038\/nrm3896","article-title":"Mechanotransduction and extracellular matrix homeostasis","volume":"15","author":"Humphrey","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.xpro.2025.104288_bib23","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.semcdb.2017.05.014","article-title":"Stretch-induced actomyosin contraction in epithelial tubes: Mechanotransduction pathways for tubular homeostasis","volume":"71","author":"Sethi","year":"2017","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.xpro.2025.104288_bib24","doi-asserted-by":"crossref","first-page":"550","DOI":"10.4049\/jimmunol.180.1.550","article-title":"Activation of Rho kinase by TNF-alpha is required for JNK activation in human pulmonary microvascular endothelial cells","volume":"180","author":"Mong","year":"2008","journal-title":"J. Immunol."},{"key":"10.1016\/j.xpro.2025.104288_bib25","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1124\/mol.115.097857","article-title":"G Protein-Coupled Receptor and RhoA-Stimulated Transcriptional Responses: Links to Inflammation, Differentiation, and Cell Proliferation","volume":"88","author":"Yu","year":"2015","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.xpro.2025.104288_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0008045","article-title":"Direct Activation of RhoA by Reactive Oxygen Species Requires a Redox-Sensitive Motif","volume":"4","author":"Aghajanian","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2025.104288_bib27","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1471-4159.2005.03179.x","article-title":"Nuclear translocation of the SRF co-activator MAL in cortical neurons: role of RhoA signalling","volume":"94","author":"Tabuchi","year":"2005","journal-title":"J. Neurochem."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S266616672500694X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S266616672500694X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T20:57:31Z","timestamp":1783025851000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S266616672500694X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["S266616672500694X"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104288","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104288","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104288"}}