{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:24:23Z","timestamp":1786998263800,"version":"build-2736575974"},"reference-count":16,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017552","name":"University of Manitoba College of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017552","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["37784"],"award-info":[{"award-number":["37784"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008794","name":"Research Manitoba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008795","name":"Manitoba Medical Service Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.xpro.2025.104318","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:00:55Z","timestamp":1767812455000},"page":"104318","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protocol for multi-organ isolation of murine leukocytes for flow cytometry"],"prefix":"10.1016","volume":"7","author":[{"given":"Paul Jerard","family":"Layug","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Marcinow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sontag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asher A.","family":"Mendelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0220-0076","authenticated-orcid":false,"given":"Janilyn","family":"Arsenio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2025.104318_bib1","doi-asserted-by":"crossref","first-page":"521957","DOI":"10.1155\/2015\/521957","article-title":"Functional and Phenotypic Plasticity of CD4 + T Cell Subsets","volume":"2015","author":"Caza","year":"2015","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.xpro.2025.104318_bib2","series-title":"T Helper Cell Differentiation and Their Function","year":"2014"},{"key":"10.1016\/j.xpro.2025.104318_bib3","doi-asserted-by":"crossref","first-page":"E6447","DOI":"10.1073\/pnas.1615590114","article-title":"Diverse continuum of CD4 + T-cell states is determined by hierarchical additive integration of cytokine signals","volume":"114","author":"Eizenberg-Magar","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2025.104318_bib4","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/imr.12032","article-title":"Early signaling events that underlie fate decisions of naive CD 4 + T cells toward distinct T-helper cell subsets","volume":"252","author":"Yamane","year":"2013","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.xpro.2025.104318_bib5","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1038\/s12276-023-01105-x","article-title":"CD8 T-cell subsets: heterogeneity, functions, and therapeutic potential","volume":"55","author":"Koh","year":"2023","journal-title":"Exp. Mol. Med."},{"key":"10.1016\/j.xpro.2025.104318_bib6","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/nature04444","article-title":"Restoring function in exhausted CD8 T cells during chronic viral infection","volume":"439","author":"Barber","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2025.104318_bib7","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.3390\/cells11111754","article-title":"Efficient Isolation of Lymphocytes and Myogenic Cells from the Tissue of Muscle Regeneration","volume":"11","author":"Kitajima","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.xpro.2025.104318_bib8","series-title":"Immunometabolism: Methods and Protocols Methods in Molecular Biology","article-title":"Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq","author":"Arsenio","year":"2020"},{"key":"10.1016\/j.xpro.2025.104318_bib9","series-title":"Dendritic Cell Protocols Methods in Molecular Medicine","first-page":"3","article-title":"Isolation of Dendritic Cells from Mouse Lymph Nodes","author":"Gabrilovich","year":"2001"},{"key":"10.1016\/j.xpro.2025.104318_bib10","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1002\/hep.28130","article-title":"Hepatic stellate cells regulate liver immunity to visceral leishmaniasis through P110\u03b4-dependent induction and expansion of regulatory T cells in mice","volume":"63","author":"Khadem","year":"2016","journal-title":"Hepatology"},{"key":"10.1016\/j.xpro.2025.104318_bib11","article-title":"Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis","volume":"160","author":"Liao","year":"2020","journal-title":"JoVE J."},{"key":"10.1016\/j.xpro.2025.104318_bib12","doi-asserted-by":"crossref","DOI":"10.1093\/discim\/kyad009","article-title":"Comparative phenotype of circulating versus tissue immune cells in human lung and blood compartments during health and disease","volume":"2","author":"Colombo","year":"2023","journal-title":"Discov. Immunol."},{"key":"10.1016\/j.xpro.2025.104318_bib13","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.1021\/acs.jproteome.3c00224","article-title":"Metabolic and Proteomic Divergence Is Present in Circulating Monocytes and Tissue-Resident Macrophages from Berkeley Sickle Cell Anemia and \u03b2-Thalassemia Mice","volume":"22","author":"Lisk","year":"2023","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.xpro.2025.104318_bib14","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1016\/j.immuni.2024.05.023","article-title":"The tissue-resident regulatory T cell pool is shaped by transient multi-tissue migration and a conserved residency program","volume":"57","author":"Burton","year":"2024","journal-title":"Immunity"},{"key":"10.1016\/j.xpro.2025.104318_bib15","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.25234.1","article-title":"Tissue-resident lymphocytes: weaponized sentinels at barrier surfaces","volume":"9","author":"Belz","year":"2020","journal-title":"F1000Res."},{"key":"10.1016\/j.xpro.2025.104318_bib16","series-title":"T-helper Cells Methods in Molecular Biology","first-page":"27","article-title":"T Cell Isolation from Mouse Kidneys","author":"Ludwig-Portugall","year":"2014"}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725007245?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166725007245?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T20:57:19Z","timestamp":1783025839000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166725007245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["S2666166725007245"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104318","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for multi-organ isolation of murine leukocytes for flow cytometry","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2025.104318","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104318"}}