{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:01:07Z","timestamp":1792364467477,"version":"4.4.0"},"reference-count":17,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.xpro.2026.104464","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T12:49:49Z","timestamp":1774442989000},"page":"104464","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protocol for isolating viable human central nervous system T cells"],"prefix":"10.1016","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3766-3241","authenticated-orcid":false,"given":"Hendrik J.","family":"Engelenburg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost","family":"Smolders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Huitinga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Hamann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Chih","family":"Hsiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2026.104464_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2025.115960","article-title":"Acquisition of residency programs by T cells entering the human brain","volume":"44","author":"Hsiao","year":"2025","journal-title":"Cell Rep."},{"key":"10.1016\/j.xpro.2026.104464_bib2","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/eji.202048665","article-title":"White matter lesions in multiple sclerosis are enriched for CD20dim CD8+ tissue-resident memory T cells","volume":"51","author":"Hsiao","year":"2021","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.xpro.2026.104464_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.105785","article-title":"Osteopontin associates with brain TRM-cell transcriptome and compartmentalization in donors with and without multiple sclerosis","volume":"26","author":"Hsiao","year":"2023","journal-title":"iScience"},{"key":"10.1016\/j.xpro.2026.104464_bib4","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1093\/brain\/awaa117","article-title":"Tissue-resident memory T cells invade the brain parenchyma in multiple sclerosis white matter lesions","volume":"143","author":"Fransen","year":"2020","journal-title":"Brain"},{"key":"10.1016\/j.xpro.2026.104464_bib5","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.1038\/s41467-018-07053-9","article-title":"Tissue-resident memory T cells populate the human brain","volume":"9","author":"Smolders","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.xpro.2026.104464_bib6","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s40478-017-0418-8","article-title":"Isolation of primary microglia from the human post-mortem brain: effects of ante- and post-mortem variables","volume":"5","author":"Mizee","year":"2017","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.xpro.2026.104464_bib7","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1038\/s41467-019-08976-7","article-title":"Transcriptional profiling of human microglia reveals grey\u2013white matter heterogeneity and multiple sclerosis-associated changes","volume":"10","author":"van der Poel","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.xpro.2026.104464_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2023.104465","article-title":"Selective emergence of antibody-secreting cells in the multiple sclerosis brain","volume":"89","author":"Bogers","year":"2023","journal-title":"EBioMedicine"},{"key":"10.1016\/j.xpro.2026.104464_bib9","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/s41593-022-01022-8","article-title":"Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain","volume":"25","author":"Marsh","year":"2022","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.xpro.2026.104464_bib10","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/nmeth.3252","article-title":"Orchestrating high-throughput genomic analysis with Bioconductor","volume":"12","author":"Huber","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2026.104464_bib11","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1038\/nprot.2009.97","article-title":"Mapping identifiers for the integration of genomic datasets with the R\/Bioconductor package biomaRt","volume":"4","author":"Durinck","year":"2009","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.xpro.2026.104464_bib12","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2010-11-3-r25","article-title":"A scaling normalization method for differential expression analysis of RNA-seq data","volume":"11","author":"Robinson","year":"2010","journal-title":"Genome Biol."},{"key":"10.1016\/j.xpro.2026.104464_bib13","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"10.1016\/j.xpro.2026.104464_bib14","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"key":"10.1016\/j.xpro.2026.104464_bib15","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.xpro.2026.104464_bib16","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"Limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.xpro.2026.104464_bib17","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq-A Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726001176?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726001176?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:43:51Z","timestamp":1785944631000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166726001176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S2666166726001176"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104464","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for isolating viable human central nervous system T cells","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104464","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2025.115960","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104464"}}