{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:26:06Z","timestamp":1786112766261,"version":"build-2736575974"},"reference-count":21,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R24GM137787"],"award-info":[{"award-number":["R24GM137787"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.xpro.2026.104479","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T17:31:59Z","timestamp":1775583119000},"page":"104479","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol to evaluate a lineage marking system in the Drosophila testis"],"prefix":"10.1016","volume":"7","author":[{"given":"Muhammed Burak","family":"Bener","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella M.","family":"DiPippo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris M.","family":"Slepchenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8508-7461","authenticated-orcid":false,"given":"Mayu","family":"Inaba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2026.104479_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2025.113564","article-title":"Detection of dedifferentiated stem cells in the Drosophila testis","volume":"28","author":"Bener","year":"2025","journal-title":"iScience"},{"key":"10.1016\/j.xpro.2026.104479_bib2","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1126\/science.1097676","article-title":"Regeneration of male germline stem cells by spermatogonial dedifferentiation in vivo","volume":"304","author":"Brawley","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.xpro.2026.104479_bib3","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/nature02436","article-title":"Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries","volume":"428","author":"Kai","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2026.104479_bib4","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1038\/s41586-023-05960-6","article-title":"Dedifferentiation maintains melanocyte stem cells in a dynamic niche","volume":"616","author":"Sun","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2026.104479_bib5","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/ncb1826","article-title":"Mouse differentiating spermatogonia can generate germinal stem cells in vivo","volume":"11","author":"Barroca","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.xpro.2026.104479_bib6","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1016\/j.devcel.2023.08.022","article-title":"Nutrient-driven dedifferentiation of enteroendocrine cells promotes adaptive intestinal growth in Drosophila","volume":"58","author":"Nagai","year":"2023","journal-title":"Dev. Cell"},{"key":"10.1016\/j.xpro.2026.104479_bib7","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s13619-020-00053-5","article-title":"Intestinal epithelial plasticity and regeneration via cell dedifferentiation","volume":"9","author":"Liu","year":"2020","journal-title":"Cell Regen."},{"key":"10.1016\/j.xpro.2026.104479_bib8","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/nature12777","article-title":"Dedifferentiation of committed epithelial cells into stem cells in vivo","volume":"503","author":"Tata","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2026.104479_bib9","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.stem.2016.01.001","article-title":"Replacement of Lost Lgr5-Positive Stem Cells through Plasticity of Their Enterocyte-Lineage Daughters","volume":"18","author":"Tetteh","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.xpro.2026.104479_bib10","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.36095","article-title":"JNK signaling triggers spermatogonial dedifferentiation during chronic stress to maintain the germline stem cell pool in the Drosophila testis","volume":"7","author":"Herrera","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.xpro.2026.104479_bib11","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nature07386","article-title":"Centrosome misorientation reduces stem cell division during ageing","volume":"456","author":"Cheng","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2026.104479_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-026-09812-7","article-title":"Stem cells resume asymmetric division upon niche re-entry through reactivating the centrosome orientation checkpoint","author":"Bener","year":"2026","journal-title":"Commun. Biol."},{"key":"10.1016\/j.xpro.2026.104479_bib13","doi-asserted-by":"crossref","first-page":"14198","DOI":"10.1073\/pnas.1111704108","article-title":"Multiple new site-specific recombinases for use in manipulating animal genomes","volume":"108","author":"Nern","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2026.104479_bib14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s11248-019-00177-8","article-title":"Tamoxifen-independent recombination of reporter genes limits lineage tracing and mosaic analysis using CreER(T2) lines","volume":"29","author":"\u00c1lvarez-Aznar","year":"2020","journal-title":"Transgenic Res."},{"key":"10.1016\/j.xpro.2026.104479_bib15","doi-asserted-by":"crossref","first-page":"3312","DOI":"10.1073\/pnas.0611511104","article-title":"An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases","volume":"104","author":"Bischof","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2026.104479_bib16","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/5007","article-title":"Generalized lacZ expression with the ROSA26 Cre reporter strain","volume":"21","author":"Soriano","year":"1999","journal-title":"Nat. Genet."},{"key":"10.1016\/j.xpro.2026.104479_bib17","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s12195-013-0295-6","article-title":"Lineage tracing quantification reveals symmetric stem cell division in Drosophila male germline stem cells","volume":"6","author":"Salzmann","year":"2013","journal-title":"Cell. Mol. Bioeng."},{"key":"10.1016\/j.xpro.2026.104479_bib18","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.xpro.2026.104479_bib19","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1002\/wsbm.165","article-title":"Virtual Cell: computational tools for modeling in cell biology","volume":"4","author":"Resasco","year":"2012","journal-title":"Wiley Interdiscip. Rev. Syst. Biol. Med."},{"key":"10.1016\/j.xpro.2026.104479_bib20","first-page":"1","article-title":"Use of virtual cell in studies of cellular dynamics","volume":"283","author":"Slepchenko","year":"2010","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"10.1016\/j.xpro.2026.104479_bib21","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-1-4939-4017-2_4","article-title":"Live Imaging of the Drosophila Testis Stem Cell Niche","volume":"1463","author":"Greenspan","year":"2017","journal-title":"Methods Mol. Biol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726001322?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726001322?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:42:46Z","timestamp":1785944566000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166726001322"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S2666166726001322"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104479","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol to evaluate a lineage marking system in the Drosophila testis","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104479","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2025.113564","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104479"}}