{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:26:07Z","timestamp":1786112767976,"version":"build-2736575974"},"reference-count":9,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["324392634-TRR 221(B13"],"award-info":[{"award-number":["324392634-TRR 221(B13"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020639","name":"Bayerische Staatsministerium f\u00fcr Wirtschaft, Landesentwicklung und Energie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020639","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.xpro.2026.104519","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T18:49:30Z","timestamp":1777056570000},"page":"104519","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells"],"prefix":"10.1016","volume":"7","author":[{"given":"Christina","family":"Pfab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas M.","family":"Holzinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Hirsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina U.","family":"Roithmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Gessner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8952-290X","authenticated-orcid":false,"given":"Sigrid","family":"B\u00fclow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2026.104519_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.113929","article-title":"Bactericidal\/permeability-increasing protein instructs dendritic cells to elicit Th22 cell response","volume":"43","author":"B\u00fclow","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.xpro.2026.104519_bib2","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.immuni.2015.05.018","article-title":"GM-CSF mouse bone marrow cultures comprise a heterogeneous population of CD11c+MHCII+ macrophages and dendritic cells","volume":"42","author":"Helft","year":"2015","journal-title":"Immunity"},{"key":"10.1016\/j.xpro.2026.104519_bib3","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0022-1759(98)00204-X","article-title":"An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow","volume":"223","author":"Lutz","year":"1999","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.xpro.2026.104519_bib4","doi-asserted-by":"crossref","DOI":"10.1002\/eji.202249816","article-title":"Guidelines for mouse and human DC generation","volume":"53","author":"Lutz","year":"2023","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.xpro.2026.104519_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0249117","article-title":"Generation and utilization of a HEK-293T murine GM-CSF expressing cell line","volume":"16","author":"Robinson","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2026.104519_bib6","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/ni.2025","article-title":"Border patrol: regulation of immunity, inflammation and tissue homeostasis at barrier surfaces by IL-22","volume":"12","author":"Sonnenberg","year":"2011","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.xpro.2026.104519_bib7","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/nature16460","article-title":"Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration","volume":"528","author":"Lindemans","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.xpro.2026.104519_bib8","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1365-2567.2008.02922.x","article-title":"Toll-like receptor ligand activation of murine bone marrow-derived dendritic cells","volume":"126","author":"Dearman","year":"2009","journal-title":"Immunology"},{"key":"10.1016\/j.xpro.2026.104519_bib9","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1038\/s41423-021-00753-1","article-title":"Metabolic programming in dendritic cells tailors immune responses and homeostasis","volume":"19","author":"M\u00f8ller","year":"2022","journal-title":"Cell. Mol. Immunol."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726001723?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726001723?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:43:09Z","timestamp":1785944589000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166726001723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":9,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S2666166726001723"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104519","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104519","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2024.113929","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104519"}}