{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:26:22Z","timestamp":1786112782543,"version":"build-2736575974"},"reference-count":10,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS134867"],"award-info":[{"award-number":["R01NS134867"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008635","name":"University of Texas Health Science Center at San Antonio","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008635","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.xpro.2026.104576","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:41:34Z","timestamp":1778859694000},"page":"104576","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for using an ELISA to detect total \u03b1-synuclein levels in Drosophila melanogaster lines expressing human \u03b1-synuclein point mutations"],"prefix":"10.1016","volume":"7","author":[{"given":"Marco","family":"Sciortino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Velazquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haven","family":"Tillmon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-5197","authenticated-orcid":false,"given":"Swati","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2026.104576_bib1","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/s41419-023-05672-9","article-title":"Alpha-synuclein in Parkinson\u2019s disease and other synucleinopathies: from overt neurodegeneration back to early synaptic dysfunction","volume":"14","author":"Calabresi","year":"2023","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.xpro.2026.104576_bib2","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a041645","article-title":"\u03b1-Synuclein in Parkinson\u2019s Disease: 12 Years Later","volume":"14","author":"Vekrellis","year":"2024","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.xpro.2026.104576_bib3","article-title":"Cognition Deficits in Parkinson\u2019s Disease: Mechanisms and Treatment","volume":"2020","author":"Fang","year":"2020","journal-title":"Parkinsons Dis."},{"key":"10.1016\/j.xpro.2026.104576_bib4","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S1474-4422(21)00030-2","article-title":"Challenges in the diagnosis of Parkinson\u2019s disease","volume":"20","author":"Tolosa","year":"2021","journal-title":"Lancet. Neurol."},{"key":"10.1016\/j.xpro.2026.104576_bib5","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1016\/S0140-6736(21)00218-X","article-title":"Parkinson\u2019s disease","volume":"397","author":"Bloem","year":"2021","journal-title":"Lancet"},{"key":"10.1016\/j.xpro.2026.104576_bib6","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1002\/mds.28534","article-title":"A Novel SNCA A30G Mutation Causes Familial Parkinson\u02bcs Disease","volume":"36","author":"Liu","year":"2021","journal-title":"Movement Disorders"},{"key":"10.1016\/j.xpro.2026.104576_bib7","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.bbapap.2019.03.001","article-title":"\u03b1-Synuclein misfolding and aggregation: Implications in Parkinson\u2019s disease pathogenesis","volume":"1867","author":"Mehra","year":"2019","journal-title":"Biochim. Biophys. Acta. Proteins Proteom."},{"key":"10.1016\/j.xpro.2026.104576_bib8","doi-asserted-by":"crossref","first-page":"7804","DOI":"10.1074\/jbc.M114.598607","article-title":"Familial Parkinson Disease-associated Mutations Alter the Site-specific Microenvironment and Dynamics of \u03b1-Synuclein","volume":"290","author":"Sahay","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.xpro.2026.104576_bib9","doi-asserted-by":"crossref","first-page":"159","DOI":"10.3389\/fncel.2020.00159","article-title":"Pathogenic Mutations Differentially Regulate Cell-to-Cell Transmission of \u03b1-Synuclein","volume":"14","author":"Guan","year":"2020","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.xpro.2026.104576_bib10","series-title":"Atlas of Drosophila Morphology: Wild-type and Classical Mutants","author":"Chyb","year":"2013"}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726002297?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726002297?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:47:04Z","timestamp":1785944824000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166726002297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S2666166726002297"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104576","relation":{"has-preprint":[{"id-type":"doi","id":"10.64898\/2026.01.07.698009","asserted-by":"object"}]},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for using an ELISA to detect total \u03b1-synuclein levels in Drosophila melanogaster lines expressing human \u03b1-synuclein point mutations","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104576","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104576"}}