{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:05:55Z","timestamp":1790121955608,"version":"4.0.1"},"reference-count":19,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000864","name":"The Michael J Fox Foundation for Parkinson's Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000864","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.xpro.2026.104735","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:35:43Z","timestamp":1784820943000},"page":"104735","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles"],"prefix":"10.1016","volume":"7","author":[{"given":"Fanni Annam\u00e1ria","family":"Boros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Regensburger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0408-6388","authenticated-orcid":false,"given":"Friederike","family":"Zunke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2026.104735_bib1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nrm.2017.125","article-title":"Shedding light on the cell biology of extracellular vesicles","volume":"19","author":"van Niel","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.xpro.2026.104735_bib2","doi-asserted-by":"crossref","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.xpro.2026.104735_bib3","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-75817-9","article-title":"Extracellular vesicles report on the MET status of their cells of origin regardless of the method used for their isolation","volume":"10","author":"Useckaite","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2026.104735_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/cells8070727","article-title":"Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis","volume":"8","author":"Doyle","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.xpro.2026.104735_bib5","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1021\/acs.jproteome.5c00149","article-title":"Comprehensive Comparison of Methods for Isolation of Extracellular Vesicles from Human Plasma","volume":"24","author":"Suresh","year":"2025","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.xpro.2026.104735_bib6","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Th\u00e9ry","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.xpro.2026.104735_bib7","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.xpro.2026.104735_bib8","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12140","article-title":"Formation of a protein corona on the surface of extracellular vesicles in blood plasma","volume":"10","author":"T\u00f3th","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.xpro.2026.104735_bib9","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1038\/s41556-022-00983-z","article-title":"Opportunities and challenges in studying the extracellular vesicle corona","volume":"24","author":"Buzas","year":"2022","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.xpro.2026.104735_bib10","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12399","article-title":"Uptake of extracellular vesicles into immune cells is enhanced by the protein corona","volume":"12","author":"Dietz","year":"2023","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.xpro.2026.104735_bib11","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1080\/17435889.2025.2538426","article-title":"The extracellular vesicle biomolecular corona: current insights and diagnostic potential","volume":"20","author":"Tassoni","year":"2025","journal-title":"Nanomedicine (Lond)"},{"key":"10.1016\/j.xpro.2026.104735_bib12","doi-asserted-by":"crossref","DOI":"10.1177\/20417314231174609","article-title":"Comparison of extracellular vesicle isolation processes for therapeutic applications","volume":"14","author":"Williams","year":"2023","journal-title":"J. Tissue Eng."},{"key":"10.1016\/j.xpro.2026.104735_bib13","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1186\/s12951-025-03768-2","article-title":"Advances of extracellular vesicles isolation and detection frontier technology: from heterogeneity analysis to clinical application","volume":"23","author":"Li","year":"2025","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.xpro.2026.104735_bib14","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12385","article-title":"MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research","volume":"12","author":"Lucien","year":"2023","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.xpro.2026.104735_bib15","doi-asserted-by":"crossref","first-page":"23430","DOI":"10.3402\/jev.v3.23430","article-title":"Single-step isolation of extracellular vesicles by size-exclusion chromatography","volume":"3","author":"B\u00f6ing","year":"2014","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.xpro.2026.104735_bib16","article-title":"Improved immunodetection of endogenous alpha-synuclein","volume":"6","author":"Lee","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2026.104735_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2021.100372","article-title":"Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue","volume":"2","author":"Stojkovska","year":"2021","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2026.104735_bib18","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1038\/s41598-017-02599-y","article-title":"Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research","volume":"7","author":"Vergauwen","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2026.104735_bib19","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1186\/s12964-025-02383-w","article-title":"Transmission Electron Microscopy-based characterization of Extracellular Vesicles from plasma and serum from Parkinson s Disease patients","volume":"23","author":"Weiss","year":"2025","journal-title":"Cell Commun. Signal."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726003886?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726003886?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:33:43Z","timestamp":1790120023000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166726003886"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S2666166726003886"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104735","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104735","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104735"}}