{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T16:28:07Z","timestamp":1793550487366,"version":"4.5.0"},"reference-count":64,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004955","name":"\u00d6sterreichische Forschungsf\u00f6rderungsgesellschaft","doi-asserted-by":"publisher","award":["FFG project no. 898252"],"award-info":[{"award-number":["FFG project no. 898252"]}],"id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017569","name":"Salzburger Landesregierung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["STAR Protocols"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.xpro.2026.104881","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:38:00Z","timestamp":1791387480000},"page":"104881","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protocol for isolation and cryopreservation of human peripheral blood mononuclear cells for high-resolution respirometry"],"prefix":"10.1016","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4759-3280","authenticated-orcid":false,"given":"Alejandra","family":"Romero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Tim\u00f3n-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime R.","family":"Willis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Perfler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Lindlbauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Knoflach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich","family":"Gnaiger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.xpro.2026.104881_bib1","doi-asserted-by":"crossref","first-page":"24778","DOI":"10.1073\/pnas.2005885117","article-title":"Childhood maltreatment is associated with changes in mitochondrial bioenergetics in maternal, but not in neonatal immune cells","volume":"117","author":"Gumpp","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.xpro.2026.104881_bib2","doi-asserted-by":"crossref","DOI":"10.1038\/tp.2014.44","article-title":"Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression","volume":"4","author":"Karabatsiakis","year":"2014","journal-title":"Transl. Psychiatry"},{"key":"10.1016\/j.xpro.2026.104881_bib3","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.mito.2016.08.006","article-title":"Inflammation in adult women with a history of child maltreatment: The involvement of mitochondrial alterations and oxidative stress","volume":"30","author":"Boeck","year":"2016","journal-title":"Mitochondrion"},{"key":"10.1016\/j.xpro.2026.104881_bib4","doi-asserted-by":"crossref","first-page":"9419","DOI":"10.1038\/s41598-018-26867-7","article-title":"Targeting the association between telomere length and immuno-cellular bioenergetics in female patients with Major Depressive Disorder","volume":"8","author":"Boeck","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2026.104881_bib5","doi-asserted-by":"crossref","first-page":"2887","DOI":"10.1007\/s11357-025-01813-4","article-title":"Mitochondrial dysfunction in peripheral mononuclear blood cells (PBMC) of individuals with mild cognitive impairment","volume":"48","author":"Dieter","year":"2025","journal-title":"GeroScience"},{"key":"10.1016\/j.xpro.2026.104881_bib6","doi-asserted-by":"crossref","DOI":"10.1002\/jcp.70096","article-title":"PBMCs mitochondrial respiration and its relation to immunity, fitness, and metabolic risk in the healthy elderly","volume":"240","author":"Gebhardt","year":"2025","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.xpro.2026.104881_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.cryobiol.2025.105212","article-title":"Mitochondrial respiratory analysis of cryopreserved PBMCs isolated from human blood","volume":"118","author":"Payne","year":"2025","journal-title":"Cryobiology"},{"key":"10.1016\/j.xpro.2026.104881_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2024.115745","article-title":"Cryopreserved PBMCs can be used for the analysis of mitochondrial respiration and serve as a diagnostic tool for mitochondrial diseases","volume":"698","author":"Korandov\u00e1","year":"2025","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.xpro.2026.104881_bib9","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1007\/s11357-025-01776-6","article-title":"Nervonic acid and 15-epi-PGA1 mediate systemic mitochondrial dysfunction in AD dementia","volume":"48","author":"Heimler","year":"2025","journal-title":"GeroScience"},{"key":"10.1016\/j.xpro.2026.104881_bib10","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1002\/alz.12731","article-title":"Blood-based bioenergetic profiling reveals differences in mitochondrial function associated with cognitive performance and Alzheimer's disease","volume":"19","author":"Mahapatra","year":"2023","journal-title":"Alzheimer's Dement."},{"key":"10.1016\/j.xpro.2026.104881_bib11","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/9703574","article-title":"Mitochondrial respiration in intact peripheral blood mononuclear cells and Sirtuin 3 activity in patients with movement disorders","volume":"2017","author":"Michalak","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.xpro.2026.104881_bib12","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2026.1756657","article-title":"Mitochondrial respiration in human peripheral blood mononuclear cells: methodology and influence of permeabilization and storage","volume":"13","author":"Sekine","year":"2026","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.xpro.2026.104881_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.exger.2024.112594","article-title":"The mitochondrial function of peripheral blood mononuclear cells in frail older patients","volume":"197","author":"Huang","year":"2024","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.xpro.2026.104881_bib14","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s12035-012-8300-y","article-title":"Peripheral mitochondrial dysfunction in Alzheimer\u2019s disease: Focus on lymphocytes","volume":"46","author":"Leuner","year":"2012","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.xpro.2026.104881_bib15","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-78551-4","article-title":"Cognitive impairment is associated with mitochondrial dysfunction in peripheral blood mononuclear cells of elderly population","volume":"10","author":"Apaijai","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2026.104881_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.exger.2020.111177","article-title":"Age-related fatigue is associated with reduced mitochondrial function in peripheral blood mononuclear cells","volume":"144","author":"Herpich","year":"2021","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.xpro.2026.104881_bib17","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1093\/gerona\/glac201","article-title":"Increased efficiency of mitochondrial coupling with a reduction in other mitochondrial respiratory parameters in peripheral blood mononuclear cells is observed in older adults","volume":"78","author":"Sriwichaiin","year":"2023","journal-title":"J. Gerontol."},{"key":"10.1016\/j.xpro.2026.104881_bib18","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1007\/s11357-023-00810-9","article-title":"Age-related changes in energy metabolism in peripheral mononuclear blood cells (PBMCs) and the brains of cognitively healthy seniors","volume":"46","author":"Silaidos","year":"2023","journal-title":"GeroScience"},{"key":"10.1016\/j.xpro.2026.104881_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fnagi.2024.1503246","article-title":"The mitochondrial function of peripheral blood cells in cognitive frailty patients","volume":"16","author":"Qin","year":"2024","journal-title":"Front. Aging Neurosci."},{"key":"10.1016\/j.xpro.2026.104881_bib20","doi-asserted-by":"crossref","DOI":"10.3390\/ijms262110377","article-title":"Impaired peripheral blood mononuclear cell (PBMC) mitochondrial respiration is associated with mortality and long COVID Syndrome severity in COVID-19 patients","volume":"26","author":"Charles","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.xpro.2026.104881_bib21","doi-asserted-by":"crossref","first-page":"705","DOI":"10.3390\/ijms26020705","article-title":"Acute severe hypoxia decreases mitochondrial chain Complex II respiration in human peripheral blood mononuclear cells","volume":"26","author":"Riou","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.xpro.2026.104881_bib22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/s41598-023-51009-z","article-title":"Alterations in mitochondria isolated from peripheral blood mononuclear cells and tumors of patients with epithelial ovarian cancers","volume":"14","author":"Charoenkwan","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.xpro.2026.104881_bib23","doi-asserted-by":"crossref","DOI":"10.1111\/apha.14102","article-title":"Effects of 30 days bed rest and exercise countermeasures on PBMC bioenergetics","volume":"240","author":"Buescher","year":"2024","journal-title":"Acta Physiol."},{"key":"10.1016\/j.xpro.2026.104881_bib24","doi-asserted-by":"crossref","first-page":"592","DOI":"10.4093\/dmj.2021.0132","article-title":"Effect of low-dose persistent organic pollutants on mitochondrial function: Human and in Vitro Evidence","volume":"46","author":"Kim","year":"2022","journal-title":"Diabetes Metab. J."},{"key":"10.1016\/j.xpro.2026.104881_bib25","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.exger.2015.07.015","article-title":"Blood-cell bioenergetics are associated with physical function and inflammation in overweight\/obese older adults","volume":"70","author":"Tyrrell","year":"2015","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.xpro.2026.104881_bib26","first-page":"e14489","article-title":"Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults","volume":"8","author":"Liepinsh","year":"2020","journal-title":"Phys. Rep."},{"key":"10.1016\/j.xpro.2026.104881_bib27","article-title":"Resistance exercise with different workloads have distinct effects on cellular respiration of peripheral blood mononuclear cells","volume":"10","author":"L\u00e4hteenm\u00e4ki","year":"2022","journal-title":"Phys. Rep."},{"key":"10.1016\/j.xpro.2026.104881_bib28","article-title":"Impact of maximal exercise on immune cell mobilization and bioenergetics","volume":"11","author":"Stampley","year":"2023","journal-title":"Phys. Rep."},{"key":"10.1016\/j.xpro.2026.104881_bib29","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.1002\/ejsc.12221","article-title":"Effects of concentric and eccentric exercise regimens on bioenergetic efficiency of lymphocytes in sedentary males","volume":"24","author":"Peng","year":"2024","journal-title":"Eur. J. Sport Sci."},{"key":"10.1016\/j.xpro.2026.104881_bib30","doi-asserted-by":"crossref","first-page":"E141","DOI":"10.1152\/ajpendo.00365.2021","article-title":"Extracellular flux analyses reveal differences in mitochondrial PBMC metabolism between high-fit and low-fit females","volume":"322","author":"Janssen","year":"2022","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.xpro.2026.104881_bib31","doi-asserted-by":"crossref","first-page":"E503","DOI":"10.1152\/ajpendo.00084.2019","article-title":"A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women","volume":"317","author":"Rose","year":"2019","journal-title":"American Journal of Physiology-Endocrinology and Metabolism"},{"key":"10.1016\/j.xpro.2026.104881_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e26745","article-title":"Correlation of mitochondrial respiration in platelets, peripheral blood mononuclear cells and muscle fibers","volume":"10","author":"Westerlund","year":"2024","journal-title":"Heliyon"},{"key":"10.1016\/j.xpro.2026.104881_bib33","doi-asserted-by":"crossref","DOI":"10.1111\/acel.70387","article-title":"Blood cell mitochondrial respiration increases with age and varies by sex in healthy adults","volume":"25","author":"Phang","year":"2026","journal-title":"Aging Cell"},{"key":"10.1016\/j.xpro.2026.104881_bib34","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.mito.2020.12.009","article-title":"Mitochondrial bioenergetics in leukocytes and oxidative stress in blood serum of mild to moderately depressed women","volume":"58","author":"Gumpp","year":"2021","journal-title":"Mitochondrion"},{"key":"10.1016\/j.xpro.2026.104881_bib35","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1152\/japplphysiol.00777.2018","article-title":"Peripheral blood mononuclear cells do not reflect skeletal muscle mitochondrial function or adaptation to high-intensity interval training in healthy young men","volume":"126","author":"Hedges","year":"2019","journal-title":"J. Appl. Physiol."},{"key":"10.1016\/j.xpro.2026.104881_bib36","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1038\/s41514-023-00130-4","article-title":"Mitochondrial function in peripheral blood cells across the human lifespan","volume":"10","author":"Ehinger","year":"2024","journal-title":"npj Aging"},{"key":"10.1016\/j.xpro.2026.104881_bib37","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1128\/CDLI.7.4.714-716.2000","article-title":"Viability and functional activity of cryopreserved mononuclear cells","volume":"7","author":"Weinberg","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.xpro.2026.104881_bib38","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13293-018-0193-7","article-title":"Sex-associated differences in mitochondrial function in human peripheral blood mononuclear cells (PBMCs) and brain","volume":"9","author":"Silaidos","year":"2018","journal-title":"Biol. Sex Differ."},{"key":"10.1016\/j.xpro.2026.104881_bib39","doi-asserted-by":"crossref","DOI":"10.1093\/gerona\/glae098","article-title":"Peripheral blood cells from older adults exhibit sex-associated differences in mitochondrial function","volume":"79","author":"Mahapatra","year":"2024","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"10.1016\/j.xpro.2026.104881_bib40","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1113\/EP092792","article-title":"Bioenergetic profiles and respiratory control in mitochondrial physiology: Precision analysis of oxidative phosphorylation","volume":"111","author":"Tim\u00f3n-G\u00f3mez","year":"2026","journal-title":"Exp. Physiol."},{"key":"10.1016\/j.xpro.2026.104881_bib41","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jim.2018.09.007","article-title":"Effect of delayed cell processing and cryopreservation on immunophenotyping in multicenter population studies","volume":"463","author":"Thyagarajan","year":"2018","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.xpro.2026.104881_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2021.100845","article-title":"Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood","volume":"2","author":"Cui","year":"2021","journal-title":"STAR Protoc."},{"key":"10.1016\/j.xpro.2026.104881_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.jim.2023.113514","article-title":"Impact of delayed PBMC processing on functional and genomic assays","volume":"519","author":"Yi","year":"2023","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.xpro.2026.104881_bib44","doi-asserted-by":"crossref","first-page":"9129","DOI":"10.3390\/ijms22179129","article-title":"Optimization of Blood Handling and Peripheral Blood Mononuclear Cell Cryopreservation of Low Cell Number Samples","volume":"22","author":"Hope","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.xpro.2026.104881_bib45","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1479-5876-9-26","article-title":"Shipping blood to a central laboratory in multicenter clinical trials: Effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function","volume":"9","author":"Olson","year":"2011","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.xpro.2026.104881_bib46","first-page":"88","article-title":"Optimization of Human Peripheral Blood Mononuclear Cells (PBMCs) Cryopreservation","volume":"1","author":"Nazarpour","year":"2012","journal-title":"Int. J. Mol. Cell Med."},{"key":"10.1016\/j.xpro.2026.104881_bib47","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3390\/cells1030313","article-title":"Optimal Thawing of cryopreserved peripheral blood mononuclear cells for use in high-throughput human immune monitoring studies","volume":"1","author":"Ramachandran","year":"2012","journal-title":"Cells"},{"key":"10.1016\/j.xpro.2026.104881_bib48","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s00262-012-1359-5","article-title":"Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols","volume":"62","author":"Filbert","year":"2013","journal-title":"Cancer Immun."},{"key":"10.1016\/j.xpro.2026.104881_bib49","series-title":"PBMC isolation from whole blood (Leucosep Method). CBIG-02-002, Version 1.0. Montreal Neurological Institute and Hospital","author":"Boivin","year":"2020"},{"key":"10.1016\/j.xpro.2026.104881_bib50","series-title":"Collection of Diagnostic Venous Blood Specimens","year":"2017"},{"key":"10.1016\/j.xpro.2026.104881_bib51","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ab.2018.03.003","article-title":"Analyzing mitochondrial function in human peripheral blood mononuclear cells","volume":"549","author":"Hsiao","year":"2018","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.xpro.2026.104881_bib52","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1365-2249.2010.04272.x","article-title":"Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society","volume":"163","author":"Mallone","year":"2011","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.xpro.2026.104881_bib53","first-page":"8","article-title":"Stability of mitochondrial respiration medium used in high-resolution respirometry with living and permeabilized cells","volume":"8","author":"Baglivo","year":"2024","journal-title":"Bioenerg Commun"},{"key":"10.1016\/j.xpro.2026.104881_bib54","article-title":"Cryopreservation and Bioenergetic evaluation of human peripheral blood mononuclear cells","volume":"200","author":"Dieter","year":"2023","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.xpro.2026.104881_bib55","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0076215","article-title":"Overnight resting of PBMC changes functional signatures of antigen specific T- cell responses: impact for immune monitoring within clinical trials","volume":"8","author":"Kutscher","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.xpro.2026.104881_bib56","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1111\/ijlh.12421","article-title":"Comparison of white blood cell counts by WNR, WDF, and WPC channels in Sysmex XN hematology analyzer","volume":"37","author":"Kim","year":"2015","journal-title":"Int. J. Lab. Hematol."},{"key":"10.1016\/j.xpro.2026.104881_bib57","first-page":"2","article-title":"Mitochondrial pathways and respiratory control: An introduction to OXPHOS analysis","volume":"2","author":"Gnaiger","year":"2020","journal-title":"Bioenerg Commun"},{"key":"10.1016\/j.xpro.2026.104881_bib58","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jcyt.2023.11.008","article-title":"Assessment and comparison of viability assays for cellular products","volume":"26","author":"Cai","year":"2024","journal-title":"Cytotherapy"},{"key":"10.1016\/j.xpro.2026.104881_bib59","article-title":"Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry","volume":"8","author":"Baglivo","year":"2022","journal-title":"Bioenerg Commun"},{"key":"10.1016\/j.xpro.2026.104881_bib60","article-title":"High-resolution respirometry in a small-volume chamber","volume":"221","author":"Leo","year":"2025","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.xpro.2026.104881_bib61","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/978-1-4939-7831-1_3","article-title":"High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria","volume":"1782","author":"Doerrier","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.xpro.2026.104881_bib62","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.3390\/biomedicines9121859","article-title":"Mitochondrial respiration of platelets: comparison of isolation methods","volume":"9","author":"Vernerova","year":"2021","journal-title":"Biomedicines"},{"key":"10.1016\/j.xpro.2026.104881_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.mito.2024.101890","article-title":"Choice of medium affects PBMC quantification, cell size, and downstream respiratory analysis","volume":"77","author":"Bager Christensen","year":"2024","journal-title":"Mitochondrion"},{"key":"10.1016\/j.xpro.2026.104881_bib64","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/labinvest.2013.53","article-title":"Methods for defining distinct bioenergetic profiles in platelets, lymphocytes, monocytes, and neutrophils, and the oxidative burst from human blood","volume":"93","author":"Chacko","year":"2013","journal-title":"Lab. Invest."}],"container-title":["STAR Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726005344?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666166726005344?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T15:43:11Z","timestamp":1793547791000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666166726005344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":64,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S2666166726005344"],"URL":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104881","relation":{},"ISSN":["2666-1667"],"issn-type":[{"value":"2666-1667","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protocol for isolation and cryopreservation of human peripheral blood mononuclear cells for high-resolution respirometry","name":"articletitle","label":"Article Title"},{"value":"STAR Protocols","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.xpro.2026.104881","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104881"}}