{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:40:06Z","timestamp":1791168006935,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T00:00:00Z","timestamp":1378080000000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"name":"NIH","award":["R01 HD44044"],"award-info":[{"award-number":["R01 HD44044"]}]},{"name":"NIH","award":["HD038082"],"award-info":[{"award-number":["HD038082"]}]},{"name":"NIH","award":["HD40793"],"award-info":[{"award-number":["HD40793"]}]},{"name":"NIH","award":["HD45821"],"award-info":[{"award-number":["HD45821"]}]},{"name":"NIH","award":["DK38452"],"award-info":[{"award-number":["DK38452"]}]},{"name":"National Agency for Scientific and Technological Promotion of Argentina","award":["WHO H9-TSA-037"],"award-info":[{"award-number":["WHO H9-TSA-037"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental Biology"],"published-print":{"date-parts":[[2012,9]]},"DOI":"10.1016\/j.ydbio.2012.06.017","type":"journal-article","created":{"date-parts":[[2012,6,30]],"date-time":"2012-06-30T14:48:26Z","timestamp":1341067706000},"page":"43-53","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":84,"title":["cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit"],"prefix":"10.1016","volume":"369","author":[{"given":"Dario","family":"Krapf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Chun Ruan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva V.","family":"Wertheimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Battistone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Pawlak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Sanjay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Pilder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Cuasnicu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Breton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo E.","family":"Visconti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Pt 1","key":"10.1016\/j.ydbio.2012.06.017_bib1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1042\/bj3330201","article-title":"Alteration of the isoform composition of plasma\u2013membrane-associated rat sperm alpha-l-fucosidase during late epididymal maturation: comparative characterization of the acidic and neutral isoforms","volume":"333","author":"Abascal","year":"1998","journal-title":"Biochem. J."},{"key":"10.1016\/j.ydbio.2012.06.017_bib2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/S8756-3282(00)00373-2","article-title":"Progressive increase in bone mass and development of odontomas in aging osteopetrotic c-src-deficient mice","volume":"27","author":"Amling","year":"2000","journal-title":"Bone"},{"key":"10.1016\/j.ydbio.2012.06.017_bib3","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1002\/j.1939-4640.2002.tb02243.x","article-title":"Expression and regulation of aquaporins 1, 8, and 9 in the testis, efferent ducts, and epididymis of adult rats and during postnatal development","volume":"23","author":"Badran","year":"2002","journal-title":"J. Androl."},{"key":"10.1016\/j.ydbio.2012.06.017_bib4","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1242\/jcs.03055","article-title":"Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa","volume":"119","author":"Baker","year":"2006","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ydbio.2012.06.017_bib5","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1177\/25.7.893996","article-title":"Dissociation of the mouse testis and characterization of isolated spermatogenic cells","volume":"25","author":"Bellve","year":"1977","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.ydbio.2012.06.017_bib6","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF01463929","article-title":"Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS)","volume":"105","author":"Brown","year":"1996","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.ydbio.2012.06.017_bib7","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1095\/biolreprod.107.061788","article-title":"A novel function for CRISP1 in rodent fertilization: involvement in sperm-zona pellucida interaction","volume":"77","author":"Busso","year":"2007","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ydbio.2012.06.017_bib8","first-page":"757194","article-title":"Post testicular sperm maturational changes in the bull: important role of the epididymosomes and prostasomes","volume":"2011","author":"Caballero","year":"2010","journal-title":"Vet. Med. Int."},{"key":"10.1016\/j.ydbio.2012.06.017_bib9","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1387\/ijdb.072538dc","article-title":"Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm\u2013egg interaction","volume":"52","author":"Cohen","year":"2008","journal-title":"Int. J. Dev. Biol."},{"key":"10.1016\/j.ydbio.2012.06.017_bib10","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/humupd\/dmn055","article-title":"New insights into epididymal biology and function","volume":"15","author":"Cornwall","year":"2009","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.ydbio.2012.06.017_bib11","doi-asserted-by":"crossref","first-page":"C1326","DOI":"10.1152\/ajpcell.00552.2009","article-title":"Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting","volume":"298","author":"Da Silva","year":"2010","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.ydbio.2012.06.017_bib12","doi-asserted-by":"crossref","first-page":"4336","DOI":"10.1182\/blood-2003-03-0871","article-title":"Lipid raft-associated protein sorting in exosomes","volume":"102","author":"de Gassart","year":"2003","journal-title":"Blood"},{"key":"10.1016\/j.ydbio.2012.06.017_bib13","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1242\/dev.059030","article-title":"Alk3 controls nephron number and androgen production via lineage-specific effects in intermediate mesoderm","volume":"138","author":"Di Giovanni","year":"2011","journal-title":"Development"},{"key":"10.1016\/j.ydbio.2012.06.017_bib14","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1016\/j.cellsig.2008.03.007","article-title":"c-Src trafficking and co-localization with the EGF receptor promotes EGF ligand-independent EGF receptor activation and signaling","volume":"20","author":"Donepudi","year":"2008","journal-title":"Cell. Signal."},{"key":"10.1016\/j.ydbio.2012.06.017_bib15","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1006\/bbrc.2000.3505","article-title":"Immunolocalization of AQP9 in liver, epididymis, testis, spleen, and brain","volume":"276","author":"Elkjaer","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ydbio.2012.06.017_bib16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/mrd.1013","article-title":"Prostasome-like particles are involved in the transfer of P25b from the bovine epididymal fluid to the sperm surface","volume":"59","author":"Frenette","year":"2001","journal-title":"Mol. Reprod. Dev"},{"key":"10.1016\/j.ydbio.2012.06.017_bib17","doi-asserted-by":"crossref","first-page":"95","DOI":"10.2164\/jandrol.110.010462","article-title":"Developmental expression of SRC-related tyrosine kinases in the mouse testis","volume":"32","author":"Goupil","year":"2011","journal-title":"J. Androl."},{"key":"10.1016\/j.ydbio.2012.06.017_bib18","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1016\/S0021-9258(17)41932-6","article-title":"p95, the major phosphotyrosine-containing protein in mouse spermatozoa, is a hexokinase with unique properties","volume":"269","author":"Kalab","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ydbio.2012.06.017_bib19","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/nature04417","article-title":"Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel","volume":"439","author":"Kirichok","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.ydbio.2012.06.017_bib20","doi-asserted-by":"crossref","first-page":"7977","DOI":"10.1074\/jbc.M109.085845","article-title":"Inhibition of Ser\/Thr phosphatases induces capacitation-associated signaling in the presence of Src kinase inhibitors","volume":"285","author":"Krapf","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ydbio.2012.06.017_bib21","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.ydbio.2012.06.017_bib22","article-title":"Role of FYN kinase in spermatogenesis: defects characteristic of Fyn-null sperm in mice","author":"Luo","year":"2011","journal-title":"Biol.Reprod"},{"key":"10.1016\/j.ydbio.2012.06.017_bib23","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1242\/dev.120.11.3313","article-title":"Roles of heterotrimeric and monomeric G proteins in sperm-induced activation of mouse eggs","volume":"120","author":"Moore","year":"1994","journal-title":"Development"},{"key":"10.1016\/j.ydbio.2012.06.017_bib24","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0014-5793(99)00433-0","article-title":"Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein","volume":"449","author":"Nakanishi","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ydbio.2012.06.017_bib25","doi-asserted-by":"crossref","unstructured":"Park, K.H., Kim, B.J., Kang, J., Nam, T.S., Lim, J.M., Kim, H.T., Park, J.K., Kim, Y.G., Chae, S.W., and Kim, U.H., 2011. Ca2+ signaling tools acquired from prostasomes are required for progesterone-induced sperm motility. Sci. Signal. 4, ra31.","DOI":"10.1126\/scisignal.2001595"},{"key":"10.1016\/j.ydbio.2012.06.017_bib26","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1095\/biolreprod65.2.384","article-title":"Aquaporin 9 expression along the male reproductive tract","volume":"65","author":"Pastor-Soler","year":"2001","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ydbio.2012.06.017_bib27","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1095\/biolreprod66.6.1716","article-title":"Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens","volume":"66","author":"Pastor-Soler","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ydbio.2012.06.017_bib28","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1530\/REP-09-0284","article-title":"Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones","volume":"139","author":"Pastor-Soler","year":"2010","journal-title":"Reproduction"},{"issue":"1","key":"10.1016\/j.ydbio.2012.06.017_bib50","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1095\/biolreprod.105.043752","article-title":"Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis","volume":"74","author":"Pietrement","year":"2006","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ydbio.2012.06.017_bib29","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1074\/jbc.M704678200","article-title":"Role of NHERF1, cystic fibrosis transmembrane conductance regulator, and cAMP in the regulation of aquaporin 9","volume":"283","author":"Pietrement","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ydbio.2012.06.017_bib30","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1006\/dbio.1993.1270","article-title":"Hybrid sterility-6: a mouse t complex locus controlling sperm flagellar assembly and movement","volume":"159","author":"Pilder","year":"1993","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ydbio.2012.06.017_bib31","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1021\/pr800796j","article-title":"Use of differential isotopic labeling and mass spectrometry to analyze capacitation-associated changes in the phosphorylation status of mouse sperm proteins","volume":"8","author":"Platt","year":"2009","journal-title":"J. Proteome. Res."},{"key":"10.1016\/j.ydbio.2012.06.017_bib32","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1095\/biolreprod.105.049304","article-title":"Lipid remodeling of murine epididymosomes and spermatozoa during epididymal maturation","volume":"74","author":"Rejraji","year":"2006","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ydbio.2012.06.017_bib33","series-title":"Physiology of Reproduction","first-page":"1071","article-title":"The epididymis","author":"Robaire","year":"2006"},{"key":"10.1016\/j.ydbio.2012.06.017_bib34","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1101\/gad.11.21.2835","article-title":"Rescue of osteoclast function by transgenic expression of kinase-deficient Src in src-\/- mutant mice","volume":"11","author":"Schwartzberg","year":"1997","journal-title":"Genes Dev."},{"key":"10.1016\/j.ydbio.2012.06.017_bib35","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1016\/j.cell.2008.10.020","article-title":"Transepithelial projections from basal cells are luminal sensors in pseudostratified epithelia","volume":"135","author":"Shum","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.ydbio.2012.06.017_bib36","doi-asserted-by":"crossref","first-page":"576","DOI":"10.2164\/jandrol.111.012971","article-title":"Establishment of cell\u2013cell cross talk in the epididymis: control of luminal acidification","volume":"32","author":"Shum","year":"2011","journal-title":"J. Androl."},{"key":"10.1016\/j.ydbio.2012.06.017_bib37","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/0092-8674(91)90499-O","article-title":"Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice","volume":"64","author":"Soriano","year":"1991","journal-title":"Cell"},{"key":"10.1016\/j.ydbio.2012.06.017_bib38","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/j.1745-7262.2007.00281.x","article-title":"Epididymosomes are involved in the acquisition of new sperm proteins during epididymal transit","volume":"9","author":"Sullivan","year":"2007","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.ydbio.2012.06.017_bib39","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1242\/jcs.114.9.1665","article-title":"A putative, ubiquitin-dependent mechanism for the recognition and elimination of defective spermatozoa in the mammalian epididymis","volume":"114","author":"Sutovsky","year":"2001","journal-title":"J. Cell. Sci."},{"key":"10.1016\/j.ydbio.2012.06.017_bib40","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.mce.2003.10.069","article-title":"Antioxidant strategies in the epididymis","volume":"216","author":"Vernet","year":"2004","journal-title":"Mol. Cell Endocrinol."},{"key":"10.1016\/j.ydbio.2012.06.017_bib41","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/aja.2010.69","article-title":"Ion channels, phosphorylation and mammalian sperm capacitation","volume":"13","author":"Visconti","year":"2011","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.ydbio.2012.06.017_bib42","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1242\/dev.121.4.1139","article-title":"Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway","volume":"121","author":"Visconti","year":"1995","journal-title":"Development"},{"key":"10.1016\/j.ydbio.2012.06.017_bib43","doi-asserted-by":"crossref","first-page":"35539","DOI":"10.1074\/jbc.M804586200","article-title":"Chloride is essential for capacitation and for the capacitation-associated increase in tyrosine phosphorylation","volume":"283","author":"Wertheimer","year":"2008","journal-title":"J. Biol. Chem."}],"container-title":["Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160612003466?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160612003466?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T04:28:26Z","timestamp":1757910506000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0012160612003466"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["S0012160612003466"],"URL":"https:\/\/doi.org\/10.1016\/j.ydbio.2012.06.017","relation":{},"ISSN":["0012-1606"],"issn-type":[{"value":"0012-1606","type":"print"}],"subject":[],"published":{"date-parts":[[2012,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit","name":"articletitle","label":"Article Title"},{"value":"Developmental Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ydbio.2012.06.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}