{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:09:16Z","timestamp":1761664156499,"version":"3.44.0"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,14]],"date-time":"2020-11-14T00:00:00Z","timestamp":1605312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental Biology"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.ydbio.2020.11.001","type":"journal-article","created":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T11:12:15Z","timestamp":1605265935000},"page":"21-36","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Zebrafish Cdx1b modulates epithalamic asymmetry by regulating ndr2 and lft1 expression"],"prefix":"10.1016","volume":"470","author":[{"given":"Chun-Shiu","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Fen","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hsiu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Jen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2554-3821","authenticated-orcid":false,"given":"Sheng-Ping L.","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ydbio.2020.11.001_bib1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.devcel.2006.10.004","article-title":"Temporally regulated asymmetric neurogenesis causes left-right difference in the zebrafish habenular structures","volume":"12","author":"Aizawa","year":"2007","journal-title":"Dev. Cell"},{"key":"10.1016\/j.ydbio.2020.11.001_bib2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1749-8104-3-9","article-title":"Brain asymmetry is encoded at the level of axon terminal morphology","volume":"3","author":"Bianco","year":"2008","journal-title":"Neural Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib3","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1098\/rstb.2008.0213","article-title":"The habenular nuclei: a conserved asymmetric relay station in the vertebrate brain","volume":"364","author":"Bianco","year":"2009","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.ydbio.2020.11.001_bib4","doi-asserted-by":"crossref","first-page":"3567","DOI":"10.1242\/dev.127.16.3567","article-title":"Multiple pathways in the midline regulate concordant brain, heart and gut left-right asymmetry","volume":"127","author":"Bisgrove","year":"2000","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib5","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.neuron.2007.07.007","article-title":"Wnt\/Axin 1\/\u03b2-Catenin signaling regulates asymmetric nodal activation, elaboration, and concordance of CNS asymmetries","volume":"55","author":"Carl","year":"2007","journal-title":"Neuron"},{"key":"10.1016\/j.ydbio.2020.11.001_bib6","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1002\/dvdy.21908","article-title":"Zebrafish cdx1b regulates differentiation of various intestinal cell lineages","volume":"238","author":"Chen","year":"2009","journal-title":"Dev. Dynam."},{"key":"10.1016\/j.ydbio.2020.11.001_bib7","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1242\/dev.010595","article-title":"Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation","volume":"135","author":"Cheng","year":"2008","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib8","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1242\/dev.083709","article-title":"Fgf signaling governs cell fate in the zebrafish pineal complex","volume":"140","author":"Clanton","year":"2013","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib9","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0896-6273(00)00120-3","article-title":"A\u00a0nodal signaling pathway regulates the laterality of neuroanatomical asymmetries in the zebrafish forebrain","volume":"28","author":"Concha","year":"2000","journal-title":"Neuron"},{"key":"10.1016\/j.ydbio.2020.11.001_bib10","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.semcdb.2008.11.007","article-title":"Mechanisms of directional asymmetry in the zebrafish epithalamus","volume":"20","author":"Concha","year":"2009","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.ydbio.2020.11.001_bib11","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.ydbio.2006.01.003","article-title":"The caudal-related homeobox genes cdx1a and cdx4 act redundantly to regulate hox gene expression and the formation of putative hematopoietic stem cells during zebrafish embryogenesis","volume":"292","author":"Davidson","year":"2006","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ydbio.2020.11.001_bib12","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1146\/annurev-genet-112414-055322","article-title":"Asymmetry of the brain: development and implications","volume":"49","author":"Duboc","year":"2015","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.ydbio.2020.11.001_bib13","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1242\/dev.01663","article-title":"Kupffer\u2019s vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut","volume":"132","author":"Essner","year":"2005","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib14","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1242\/dev.127.5.1081","article-title":"Mesendoderm and left-right brain, heart and gut development are differentially regulated by pitx2 isoforms","volume":"127","author":"Essner","year":"2000","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib15","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/26013","article-title":"Zebrafish organizer development and germ-layer formation require nodal-related signals","volume":"395","author":"Feldman","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.ydbio.2020.11.001_bib16","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1053\/j.gastro.2008.07.024","article-title":"Intestinal differentiation in zebrafish requires Cdx1b, a functional equivalent of mammalian Cdx2","volume":"135","author":"Flores","year":"2008","journal-title":"Gastroenterology"},{"key":"10.1016\/j.ydbio.2020.11.001_bib17","doi-asserted-by":"crossref","first-page":"4869","DOI":"10.1242\/dev.02046","article-title":"Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target","volume":"132","author":"Gamse","year":"2005","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib18","doi-asserted-by":"crossref","first-page":"E3435","DOI":"10.1073\/pnas.1302581110","article-title":"Two distinct forms of functional lateralization in the human brain","volume":"110","author":"Gotts","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ydbio.2020.11.001_bib19","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0092-8674(00)80720-5","article-title":"The EGF-CFC protein one-eyed pinhead is essential for nodal signaling","volume":"97","author":"Gritsman","year":"1999","journal-title":"Cell"},{"key":"10.1016\/j.ydbio.2020.11.001_bib20","doi-asserted-by":"crossref","first-page":"593","DOI":"10.4161\/cbt.3.7.913","article-title":"The role of Cdx proteins in intestinal development and cancer","volume":"3","author":"Guo","year":"2004","journal-title":"Canc. Biol. Ther."},{"key":"10.1016\/j.ydbio.2020.11.001_bib21","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.neuron.2007.06.037","article-title":"Six3 represses nodal activity to establish early brain asymmetry in zebrafish","volume":"55","author":"Inbal","year":"2007","journal-title":"Neuron"},{"key":"10.1016\/j.ydbio.2020.11.001_bib22","doi-asserted-by":"crossref","first-page":"13904","DOI":"10.1073\/pnas.1308335110","article-title":"Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system","volume":"110","author":"Jao","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ydbio.2020.11.001_bib23","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1046\/j.1365-2443.2001.00481.x","article-title":"Roles of nodal-lefty regulatory loops in embryonic patterning of vertebrates","volume":"6","author":"Juan","year":"2001","journal-title":"Gene Cell."},{"key":"10.1016\/j.ydbio.2020.11.001_bib24","first-page":"2641","article-title":"A\u00a0transcription factor network controls cell migration and fate decisions in the developing zebrafish pineal complex","volume":"143","author":"Khuansuwan","year":"2016","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib25","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/aja.1002030302","article-title":"Stages of embryonic development of the zebrafish","volume":"203","author":"Kimmel","year":"1995","journal-title":"Dev. Dynam."},{"key":"10.1016\/j.ydbio.2020.11.001_bib26","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1242\/dev.02791","article-title":"Neuropilin asymmetry mediates a left-right difference in habenular connectivity","volume":"134","author":"Kuan","year":"2007","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib27","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.1242\/dev.127.23.5101","article-title":"Asymmetric nodal signaling in the zebrafish diencephalon positions the pineal organ","volume":"127","author":"Liang","year":"2000","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib28","doi-asserted-by":"crossref","first-page":"103598","DOI":"10.1016\/j.mod.2020.103598","article-title":"Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchia cartilage development","author":"Liu","year":"2020","journal-title":"Mech. Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib29","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1242\/dev.00436","article-title":"The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry","volume":"130","author":"Long","year":"2003","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib30","doi-asserted-by":"crossref","first-page":"3069","DOI":"10.1007\/s00018-012-0985-6","article-title":"Left-right asymmetry in zebrafish","volume":"69","author":"Matsui","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.ydbio.2020.11.001_bib31","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S1097-2765(00)80331-7","article-title":"Mouse Lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation","volume":"4","author":"Meno","year":"1999","journal-title":"Mol. Cell."},{"key":"10.1016\/j.ydbio.2020.11.001_bib32","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1038\/nmeth.3543","article-title":"CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo","volume":"12","author":"Moreno-Mateos","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ydbio.2020.11.001_bib33","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1093\/bioinformatics\/btu743","article-title":"CRISPRdirect: software for designing CRISPR\/Cas guide RNA with reduced off-target sites","volume":"31","author":"Naito","year":"2015","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ydbio.2020.11.001_bib34","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1101\/gad.13.12.1575","article-title":"Asymmetric and node-specific nodal expression patterns are controlled by two distinct cis-acting regulatory elements","volume":"13","author":"Norris","year":"1999","journal-title":"Genes Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib35","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.28635","article-title":"Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning","volume":"6","author":"Pelliccia","year":"2017","journal-title":"Elife"},{"key":"10.1016\/j.ydbio.2020.11.001_bib36","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1006\/dbio.1998.8935","article-title":"Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry","volume":"199","author":"Rebagliati","year":"1998","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ydbio.2020.11.001_bib37","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.neuron.2008.11.030","article-title":"An Fgf 8-dependent bistable cell migratory event establishes CNS asymmetry","volume":"61","author":"Regan","year":"2009","journal-title":"Neuron"},{"key":"10.1016\/j.ydbio.2020.11.001_bib38","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.28785","article-title":"Nodal patterning without Lefty inhibitory feedback is functional but fragile","volume":"6","author":"Rogers","year":"2017","journal-title":"Elife"},{"key":"10.1016\/j.ydbio.2020.11.001_bib39","doi-asserted-by":"crossref","first-page":"E9812","DOI":"10.1073\/pnas.1812016115","article-title":"Left\/right asymmetric collective migration of parapineal cells is mediated by focal FGF signaling activity in leading cells","volume":"115","author":"Roussign\u00e9","year":"2018","journal-title":"Proc. Natl. Acad. Sci. Unit. States Am."},{"key":"10.1016\/j.ydbio.2020.11.001_bib40","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1242\/dev.034793","article-title":"Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei","volume":"136","author":"Roussigne","year":"2009","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib41","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1101\/gad.13.3.259","article-title":"Distinct transcriptional regulatory mechanisms underlie left-right asymmetric expression of lefty-1 and lefty-2","volume":"13","author":"Saijoh","year":"1999","journal-title":"Genes Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib42","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/26020","article-title":"Induction of the zebrafish ventral brain and floorplate requires cyclops\/nodal signalling","volume":"395","author":"Sampath","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.ydbio.2020.11.001_bib43","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ydbio.2004.12.007","article-title":"Interaction of Wnt and caudal-related genes in zebrafish posterior body formation","volume":"279","author":"Shimizu","year":"2005","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ydbio.2020.11.001_bib44","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S1097-2765(01)00162-9","article-title":"Two-step regulation of left-right asymmetric expression of Pitx2: initiation by nodal signaling and maintenance by Nkx 2","volume":"7","author":"Shiratori","year":"2001","journal-title":"Mol. Cell."},{"key":"10.1016\/j.ydbio.2020.11.001_bib45","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1242\/dev.002980","article-title":"Repression of the hindbrain developmental program by Cdx factors is required for the specification of the vertebrate spinal cord","volume":"134","author":"Skromne","year":"2007","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib46","series-title":"Biometry: the Principles and Practice of Statistics in Biological Research","first-page":"915","author":"Sokal","year":"2012"},{"key":"10.1016\/j.ydbio.2020.11.001_bib47","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1136\/jmg.33.6.498","article-title":"Defects in the determination of left-right asymmetry","volume":"33","author":"Splitt","year":"1996","journal-title":"J.\u00a0Med. Genet."},{"key":"10.1016\/j.ydbio.2020.11.001_bib48","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1242\/dev.038695","article-title":"Making a difference together: reciprocal interactions in C. elegans and zebrafish asymmetric neural development","volume":"137","author":"Taylor","year":"2010","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib49","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1242\/dev.126.2.229","article-title":"Antivin, a novel and divergent member of the TGFbeta superfamily, negatively regulates mesoderm induction","volume":"126","author":"Thisse","year":"1999","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib50","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1242\/dev.129.9.2181","article-title":"Cdx1 and Cdx2 have overlapping functions in anteroposterior patterning and posterior axis elongation","volume":"129","author":"van den Akker","year":"2002","journal-title":"Dev."},{"key":"10.1016\/j.ydbio.2020.11.001_bib51","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.ydbio.2014.08.011","article-title":"Maternal Vsx1 plays an essential role in regulating prechordal mesendoderm and forebrain formation in zebrafish","volume":"394","author":"Xu","year":"2014","journal-title":"Dev. Biol."}],"container-title":["Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160620302931?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160620302931?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T04:44:08Z","timestamp":1757911448000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0012160620302931"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":51,"alternative-id":["S0012160620302931"],"URL":"https:\/\/doi.org\/10.1016\/j.ydbio.2020.11.001","relation":{},"ISSN":["0012-1606"],"issn-type":[{"type":"print","value":"0012-1606"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Zebrafish Cdx1b modulates epithalamic asymmetry by regulating ndr2 and lft1 expression","name":"articletitle","label":"Article Title"},{"value":"Developmental Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ydbio.2020.11.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}