{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T05:04:14Z","timestamp":1779944654864,"version":"3.53.1"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100032572","name":"Westlake Education Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100032572","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental Biology"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.ydbio.2025.07.009","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T06:27:23Z","timestamp":1753511243000},"page":"26-38","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A low-cost, high-throughput pipeline for 3D imaging of embryonic mouse hearts using lightsheet microscopy"],"prefix":"10.1016","volume":"527","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6590-2476","authenticated-orcid":false,"given":"Xiangyang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youshi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4993-2322","authenticated-orcid":false,"given":"Hongjun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ydbio.2025.07.009_bib1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.3389\/fphys.2019.00514","article-title":"Glutaraldehyde \u2013 a subtle tool in the investigation of healthy and pathologic red blood cells","volume":"10","author":"Abay","year":"2019","journal-title":"Front. Physiol."},{"key":"10.1016\/j.ydbio.2025.07.009_bib2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/abio.2000.5006","article-title":"Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence","volume":"291","author":"Billinton","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ydbio.2025.07.009_bib3","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1161\/CIRCRESAHA.116.309302","article-title":"Changing landscape of congenital heart disease","volume":"120","author":"Bouma","year":"2017","journal-title":"Circ. Res."},{"key":"10.1016\/j.ydbio.2025.07.009_bib4","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/nature12107","article-title":"Structural and molecular interrogation of intact biological systems","volume":"497","author":"Chung","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.ydbio.2025.07.009_bib5","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1016\/j.devcel.2022.08.005","article-title":"4D reconstruction of murine developmental trajectories using spherical harmonics","volume":"57","author":"Dalmasso","year":"2022","journal-title":"Dev. Cell"},{"key":"10.1016\/j.ydbio.2025.07.009_bib6","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ydbio.2012.05.001","article-title":"Growth of the developing mouse heart: an interactive qualitative and quantitative 3D atlas","volume":"368","author":"De Boer","year":"2012","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ydbio.2025.07.009_bib7","article-title":"Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution","volume":"7","author":"Ding","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ydbio.2025.07.009_bib8","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.cell.2023.01.001","article-title":"Graded mesoderm assembly governs cell fate and morphogenesis of the early mammalian heart","volume":"186","author":"Dominguez","year":"2023","journal-title":"Cell"},{"key":"10.1016\/j.ydbio.2025.07.009_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1161\/01.RES.77.1.1","article-title":"Molecular regulation of atrioventricular valvuloseptal morphogenesis","volume":"77","author":"Eisenberg","year":"1995","journal-title":"Circ. Res."},{"key":"10.1016\/j.ydbio.2025.07.009_bib10","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.34110","article-title":"A novel source of arterial valve cells linked to bicuspid aortic valve without raphe in mice","volume":"7","author":"Eley","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.ydbio.2025.07.009_bib11","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/s44161-022-00065-1","article-title":"Pseudodynamic analysis of heart tube formation in the mouse reveals strong regional variability and early left\u2013right asymmetry","volume":"1","author":"Esteban","year":"2022","journal-title":"Nat. Cardiovasc. Res."},{"key":"10.1016\/j.ydbio.2025.07.009_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/srep22489","article-title":"Cardiac light-sheet fluorescent microscopy for multi-scale and rapid imaging of architecture and function","volume":"6","author":"Fei","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ydbio.2025.07.009_bib13","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1469-7580.2007.00838.x","article-title":"The coronary arteries of the C57BL\/6 mouse strains: implications for comparison with mutant models","volume":"212","author":"Fern\u00e1ndez","year":"2008","journal-title":"J. Anat."},{"key":"10.1016\/j.ydbio.2025.07.009_bib14","first-page":"56071","article-title":"High-resolution Episcopic Microscopy (HREM) - simple and robust protocols for processing and visualizing organic materials","author":"Geyer","year":"2017","journal-title":"JoVE J."},{"key":"10.1016\/j.ydbio.2025.07.009_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.739079","article-title":"Navigating the light-sheet image analysis software landscape: concepts for driving cohesion from data acquisition to analysis","volume":"9","author":"Gibbs","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.ydbio.2025.07.009_bib16","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1038\/nn.2928","article-title":"Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain","volume":"14","author":"Hama","year":"2011","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.ydbio.2025.07.009_bib17","doi-asserted-by":"crossref","first-page":"355","DOI":"10.52083\/FDTA2953","article-title":"A systematic review on normal and abnormal anatomy of coronary arteries","volume":"26","author":"Hegazy","year":"2022","journal-title":"EJA"},{"key":"10.1016\/j.ydbio.2025.07.009_bib18","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.77419","article-title":"EZ Clear for simple, rapid, and robust mouse whole organ clearing","volume":"11","author":"Hsu","year":"2022","journal-title":"eLife"},{"key":"10.1016\/j.ydbio.2025.07.009_bib19","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1126\/science.1100035","article-title":"Optical sectioning deep inside live embryos by selective plane illumination microscopy","volume":"305","author":"Huisken","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.ydbio.2025.07.009_bib20","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.30668.058","article-title":"Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis","author":"Ivanovitch","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.ydbio.2025.07.009_bib21","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1242\/dev.127.8.1607","article-title":"Fate of the mammalian cardiac neural crest","volume":"127","author":"Jiang","year":"2000","journal-title":"Development"},{"key":"10.1016\/j.ydbio.2025.07.009_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2021.102387","article-title":"Tissue clearing and imaging methods for cardiovascular development","volume":"24","author":"Kolesov\u00e1","year":"2021","journal-title":"iScience"},{"key":"10.1016\/j.ydbio.2025.07.009_bib23","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3389\/fnana.2019.00026","article-title":"Development of sample-adaptable holders for lightsheet microscopy","volume":"13","author":"Laroche","year":"2019","journal-title":"Front. Neuroanat."},{"key":"10.1016\/j.ydbio.2025.07.009_bib24","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.28951","article-title":"A predictive model of asymmetric morphogenesis from 3D reconstructions of mouse heart looping dynamics","volume":"6","author":"Le Garrec","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.ydbio.2025.07.009_bib25","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/s42003-020-1035-9","article-title":"Mutation of LRP1 in cardiac neural crest cells causes congenital heart defects by perturbing outflow lengthening","volume":"3","author":"Lin","year":"2020","journal-title":"Commun. Biol."},{"key":"10.1016\/j.ydbio.2025.07.009_bib26","article-title":"The developmental processes of ventricular septal defects with outflow tract malalignment","volume":"255","author":"Liu","year":"2024","journal-title":"Annal. Anatomy - Anatomischer Anzeiger"},{"key":"10.1016\/j.ydbio.2025.07.009_bib27","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1007\/s00335-022-09976-7","article-title":"Three-dimensional microCT imaging of mouse heart development from early post-implantation to late fetal stages","volume":"34","author":"Li-Villarreal","year":"2023","journal-title":"Mamm. Genome"},{"key":"10.1016\/j.ydbio.2025.07.009_bib28","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13073-024-01372-x","article-title":"ENU-based dominant genetic screen identifies contractile and neuronal gene mutations in congenital heart disease","volume":"16","author":"Luo","year":"2024","journal-title":"Genome Med."},{"key":"10.1016\/j.ydbio.2025.07.009_bib29","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/s41596-020-00414-z","article-title":"Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH","volume":"16","author":"Messal","year":"2021","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ydbio.2025.07.009_bib30","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1152\/physrev.00006.2003","article-title":"Cardiac chamber formation: development, genes, and evolution","volume":"83","author":"Moorman","year":"2003","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ydbio.2025.07.009_bib31","author":"Mu"},{"key":"10.1016\/j.ydbio.2025.07.009_bib32","doi-asserted-by":"crossref","first-page":"3599","DOI":"10.1242\/dev.025437","article-title":"An FGF autocrine loop initiated in second heart field mesoderm regulates morphogenesis at the arterial pole of the heart","volume":"135","author":"Park","year":"2008","journal-title":"Development"},{"key":"10.1016\/j.ydbio.2025.07.009_bib33","series-title":"International Review of Cytology","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0074-7696(05)43005-3","article-title":"Cell biology of cardiac cushion development","author":"Person","year":"2005"},{"key":"10.1016\/j.ydbio.2025.07.009_bib34","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3390\/jcdd10040145","article-title":"Imaging approaches and the quantitative analysis of heart development","volume":"10","author":"Raiola","year":"2023","journal-title":"JCDD"},{"key":"10.1016\/j.ydbio.2025.07.009_bib35","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.58138","article-title":"Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model","volume":"9","author":"Rykiel","year":"2020","journal-title":"eLife"},{"key":"10.1016\/j.ydbio.2025.07.009_bib36","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/s41467-018-02891-z","article-title":"Analysis of cardiomyocyte clonal expansion during mouse heart development and injury","volume":"9","author":"Sereti","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ydbio.2025.07.009_bib37","doi-asserted-by":"crossref","DOI":"10.3791\/54303","article-title":"Imaging cleared embryonic and postnatal hearts at single-cell resolution","author":"Shaikh Qureshi","year":"2016","journal-title":"JoVE J."},{"key":"10.1016\/j.ydbio.2025.07.009_bib38","doi-asserted-by":"crossref","DOI":"10.1002\/jbio.202200278","article-title":"Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice","volume":"16","author":"Sodimu","year":"2023","journal-title":"J. Biophot."},{"key":"10.1016\/j.ydbio.2025.07.009_bib39","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.cell.2014.03.042","article-title":"Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis","volume":"157","author":"Susaki","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.ydbio.2025.07.009_bib40","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/s41583-019-0250-1","article-title":"Tissue clearing and its applications in neuroscience","volume":"21","author":"Ueda","year":"2020","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.ydbio.2025.07.009_bib41","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1016\/j.jacc.2011.08.025","article-title":"Birth prevalence of congenital heart disease worldwide","volume":"58","author":"Van Der Linde","year":"2011","journal-title":"J. Am. Coll. Cardiol."},{"key":"10.1016\/j.ydbio.2025.07.009_bib42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1117\/1.JBO.20.9.090501","article-title":"Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant","volume":"20","author":"Wang","year":"2015","journal-title":"J. Biomed. Opt."}],"updated-by":[{"DOI":"10.1016\/j.ydbio.2025.09.015","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000}}],"container-title":["Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160625002003?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160625002003?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T04:28:47Z","timestamp":1779942527000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0012160625002003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":42,"alternative-id":["S0012160625002003"],"URL":"https:\/\/doi.org\/10.1016\/j.ydbio.2025.07.009","relation":{"is-supplemented-by":[{"id-type":"uri","id":"https:\/\/zenodo.org\/records\/15846349","asserted-by":"subject"}]},"ISSN":["0012-1606"],"issn-type":[{"value":"0012-1606","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A low-cost, high-throughput pipeline for 3D imaging of embryonic mouse hearts using lightsheet microscopy","name":"articletitle","label":"Article Title"},{"value":"Developmental Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ydbio.2025.07.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}