{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:35:32Z","timestamp":1790789732554,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Semmy Foundation"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Cell Research"],"published-print":{"date-parts":[[2017,11]]},"DOI":"10.1016\/j.yexcr.2017.09.032","type":"journal-article","created":{"date-parts":[[2017,9,22]],"date-time":"2017-09-22T18:16:23Z","timestamp":1506104183000},"page":"397-403","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":38,"title":["Culture methods of diffuse intrinsic pontine glioma cells determine response to targeted therapies"],"prefix":"10.1016","volume":"360","author":[{"given":"Micha\u00ebl H.","family":"Meel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.Charlotte P.","family":"Sewing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Waranecki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis S.","family":"Metselaar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurine E.","family":"Wedekind","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Koster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dannis G.","family":"van Vuurden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gertjan J.L.","family":"Kaspers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Hulleman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.yexcr.2017.09.032_bib1","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1200\/JCO.2005.04.6599","article-title":"Advances toward an understanding of brainstem gliomas","volume":"24","author":"Donaldson","year":"2006","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib2","doi-asserted-by":"crossref","DOI":"10.1093\/neuonc\/now101","article-title":"Pediatric high-grade glioma: biologically and clinically in need of new thinking","author":"Jones","year":"2016","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib3","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s00401-012-0998-0","article-title":"K27M mutation in histone H3.3 defines clinically and biologically distinct subgroups of pediatric diffuse intrinsic pontine gliomas","volume":"124","author":"Khuong-Quang","year":"2012","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/ng.1102","article-title":"Somatic histone H3 alterations in pediatric diffuse intrinsic pontine gliomas and non-brainstem glioblastomas","volume":"44","author":"Wu","year":"2012","journal-title":"Nat. Genet"},{"key":"10.1016\/j.yexcr.2017.09.032_bib5","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1007\/s00401-015-1478-0","article-title":"Histone H3F3A and HIST1H3B K27M mutations define two subgroups of diffuse intrinsic pontine gliomas with different prognosis and phenotypes","volume":"130","author":"Castel","year":"2015","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib6","doi-asserted-by":"crossref","first-page":"11185","DOI":"10.1038\/ncomms11185","article-title":"Spatial and temporal homogeneity of driver mutations in diffuse intrinsic pontine glioma","volume":"7","author":"Nikbakht","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.yexcr.2017.09.032_bib7","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/ng.2936","article-title":"Genomic analysis of diffuse intrinsic pontine gliomas identifies three molecular subgroups and recurrent activating ACVR1 mutations","volume":"46","author":"Buczkowicz","year":"2014","journal-title":"Nat. Genet."},{"key":"10.1016\/j.yexcr.2017.09.032_bib8","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/ng.2925","article-title":"Recurrent activating ACVR1 mutations in diffuse intrinsic pontine glioma","volume":"46","author":"Taylor","year":"2014","journal-title":"Nat. Genet."},{"key":"10.1016\/j.yexcr.2017.09.032_bib9","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nm.3855","article-title":"Functionally defined therapeutic targets in diffuse intrinsic pontine glioma","volume":"21","author":"Grasso","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.yexcr.2017.09.032_bib10","doi-asserted-by":"crossref","first-page":"3999","DOI":"10.1200\/JCO.2011.35.5677","article-title":"Genome-wide analyses identify recurrent amplifications of receptor tyrosine kinases and cell-cycle regulatory genes in diffuse intrinsic pontine glioma","volume":"29","author":"Paugh","year":"2011","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib11","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1093\/neuonc\/nor190","article-title":"Genomic aberrations in pediatric diffuse intrinsic pontine gliomas","volume":"14","author":"Warren","year":"2012","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib12","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/pbc.24060","article-title":"Critical oncogenic mutations in newly diagnosed pediatric diffuse intrinsic pontine glioma","volume":"58","author":"Grill","year":"2012","journal-title":"Pediatr. Blood Cancer"},{"key":"10.1016\/j.yexcr.2017.09.032_bib13","doi-asserted-by":"crossref","first-page":"6219","DOI":"10.1158\/0008-5472.CAN-13-1491","article-title":"Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas","volume":"73","author":"Paugh","year":"2013","journal-title":"Cancer Res."},{"key":"10.1016\/j.yexcr.2017.09.032_bib14","doi-asserted-by":"crossref","first-page":"e30313","DOI":"10.1371\/journal.pone.0030313","article-title":"Mesenchymal transition and PDGFRA amplification\/mutation are key distinct oncogenic events in pediatric diffuse intrinsic pontine gliomas","volume":"7","author":"Puget","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.yexcr.2017.09.032_bib15","first-page":"5821","article-title":"Identification of a cancer stem cell in human brain tumors","volume":"63","author":"Singh","year":"2003","journal-title":"Cancer Res."},{"key":"10.1016\/j.yexcr.2017.09.032_bib16","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jneumeth.2008.07.022","article-title":"An efficient method for derivation and propagation of glioblastoma cell lines that conserves the molecular profile of their original tumours","volume":"176","author":"Fael Al-Mayhani","year":"2009","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.yexcr.2017.09.032_bib17","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1093\/neuonc\/nor195","article-title":"Maintenance of primary tumor phenotype and genotype in glioblastoma stem cells","volume":"14","author":"Wakimoto","year":"2012","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib18","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1038\/sj.onc.1210850","article-title":"The genomic profile of human malignant glioma is altered early in primary cell culture and preserved in spheroids","volume":"27","author":"De Witt Hamer","year":"2008","journal-title":"Oncogene"},{"key":"10.1016\/j.yexcr.2017.09.032_bib19","doi-asserted-by":"crossref","DOI":"10.18632\/oncotarget.11676","article-title":"Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media","author":"Garcia-Romero","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.yexcr.2017.09.032_bib20","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1186\/s12885-016-2690-6","article-title":"Polo-like Kinase 1 as a potential therapeutic target in diffuse intrinsic pontine glioma","volume":"16","author":"Amani","year":"2016","journal-title":"BMC. Cancer"},{"key":"10.1016\/j.yexcr.2017.09.032_bib21","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/j.1750-3639.2012.00633.x","article-title":"Aurora kinase B is a potential therapeutic target in pediatric diffuse intrinsic pontine glioma","volume":"23","author":"Buczkowicz","year":"2013","journal-title":"Brain Pathol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib22","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.1158\/1535-7163.MCT-15-0282","article-title":"Poly-ADP-ribose polymerase as a therapeutic target in pediatric diffuse intrinsic pontine glioma and pediatric high-grade astrocytoma","volume":"14","author":"Chornenkyy","year":"2015","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.yexcr.2017.09.032_bib23","doi-asserted-by":"crossref","first-page":"e0118926","DOI":"10.1371\/journal.pone.0118926","article-title":"A high-throughput in vitro drug screen in a genetically engineered mouse model of diffuse intrinsic pontine glioma identifies BMS-754807 as a promising therapeutic agent","volume":"10","author":"Halvorson","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.yexcr.2017.09.032_bib24","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1097\/NEN.0000000000000216","article-title":"Disrupting NOTCH slows diffuse intrinsic pontine glioma growth, enhances radiation sensitivity, and shows combinatorial efficacy with bromodomain inhibition","volume":"74","author":"Taylor","year":"2015","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib25","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1158\/1535-7163.MCT-12-0735","article-title":"WEE1 kinase inhibition enhances the radiation response of diffuse intrinsic pontine gliomas","volume":"12","author":"Caretti","year":"2013","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.yexcr.2017.09.032_bib26","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1093\/neuonc\/not220","article-title":"Targeting Wee1 for the treatment of pediatric high-grade gliomas","volume":"16","author":"Mueller","year":"2014","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib27","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1093\/neuonc\/nou330","article-title":"Preclinical evaluation of dasatinib alone and in combination with cabozantinib for the treatment of diffuse intrinsic pontine glioma","volume":"17","author":"Truffaux","year":"2015","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib28","doi-asserted-by":"crossref","first-page":"e61512","DOI":"10.1371\/journal.pone.0061512","article-title":"In vitro drug response and efflux transporters associated with drug resistance in pediatric high grade glioma and diffuse intrinsic pontine glioma","volume":"8","author":"Veringa","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.yexcr.2017.09.032_bib29","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1055\/s-0042-105292","article-title":"No significant cytotoxic effect of the EZH2 Inhibitor Tazemetostat (EPZ-6438) on pediatric glioma cells with wildtype histone 3 or mutated histone 3.3","volume":"228","author":"Wiese","year":"2016","journal-title":"Klin. Padiatr."},{"key":"10.1016\/j.yexcr.2017.09.032_bib30","doi-asserted-by":"crossref","first-page":"2166","DOI":"10.1158\/1535-7163.MCT-15-0558","article-title":"Bevacizumab targeting diffuse intrinsic pontine glioma: results of 89Zr-Bevacizumab PET imaging in brain tumor models","volume":"15","author":"Jansen","year":"2016","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.yexcr.2017.09.032_bib31","doi-asserted-by":"crossref","first-page":"7900","DOI":"10.1021\/jm501177w","article-title":"Structure-activity relationship of 3,5-diaryl-2-aminopyridine ALK2 inhibitors reveals unaltered binding affinity for fibrodysplasia ossificans progressiva causing mutants","volume":"57","author":"Mohedas","year":"2014","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.yexcr.2017.09.032_bib32","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1021\/jm048995+","article-title":"Structure-activity relationship studies of salinosporamide A (NPI-0052), a novel marine derived proteasome inhibitor","volume":"48","author":"Macherla","year":"2005","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.yexcr.2017.09.032_bib33","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.ccr.2006.03.030","article-title":"Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines","volume":"9","author":"Lee","year":"2006","journal-title":"Cancer Cell"},{"key":"10.1016\/j.yexcr.2017.09.032_bib34","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/ijc.2910540118","article-title":"Proliferation, migration and invasion of human glioma cells exposed to antifolate drugs","volume":"54","author":"Terzis","year":"1993","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.yexcr.2017.09.032_bib35","doi-asserted-by":"crossref","first-page":"E33","DOI":"10.1002\/ijc.27349","article-title":"In vitro and in vivo characterization of a novel Hedgehog signaling antagonist in human glioblastoma cell lines","volume":"131","author":"Ferruzzi","year":"2012","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.yexcr.2017.09.032_bib36","doi-asserted-by":"crossref","first-page":"3027","DOI":"10.1634\/stemcells.2007-1073","article-title":"Brain cancer stem cells display preferential sensitivity to Akt inhibition","volume":"26","author":"Eyler","year":"2008","journal-title":"Stem Cells"},{"key":"10.1016\/j.yexcr.2017.09.032_bib37","doi-asserted-by":"crossref","first-page":"219","DOI":"10.5114\/fn.2015.54422","article-title":"The effect of neurosphere culture conditions on the cellular metabolism of glioma cells","volume":"53","author":"Kahlert","year":"2015","journal-title":"Folia Neuropathol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib38","doi-asserted-by":"crossref","first-page":"25","DOI":"10.5115\/acb.2015.48.1.25","article-title":"Neurosphere and adherent culture conditions are equivalent for malignant glioma stem cell lines","volume":"48","author":"Rahman","year":"2015","journal-title":"Anat. Cell Biol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib39","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1093\/neuonc\/nou329","article-title":"Akt- or MEK-mediated mTOR inhibition suppresses Nf1 optic glioma growth","volume":"17","author":"Kaul","year":"2015","journal-title":"Neuro Oncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib40","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1002\/pbc.25579","article-title":"Inhibition of MEK confers hypersensitivity to X-radiation in the context of BRAF mutation in a model of childhood astrocytoma","volume":"62","author":"Studebaker","year":"2015","journal-title":"Pediatr. Blood Cancer"},{"key":"10.1016\/j.yexcr.2017.09.032_bib41","first-page":"1","article-title":"BRAF and MEK inhibitors in pediatric glioma: new therapeutic strategies, new toxicities","author":"Rizzo","year":"2016","journal-title":"Expert. Opin. Drug Metab. Toxicol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib42","doi-asserted-by":"crossref","DOI":"10.1007\/s11060-016-2237-3","article-title":"Clinical utility and treatment outcome of comprehensive genomic profiling in high grade glioma patients","author":"Blumenthal","year":"2016","journal-title":"J. Neurooncol."},{"key":"10.1016\/j.yexcr.2017.09.032_bib43","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s12967-016-0803-2","article-title":"Suppression of RAF\/MEK or PI3K synergizes cytotoxicity of receptor tyrosine kinase inhibitors in glioma tumor-initiating cells","volume":"14","author":"Shingu","year":"2016","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.yexcr.2017.09.032_bib44","first-page":"45","article-title":"A preclinical orthotopic model for glioblastoma recapitulates key features of human tumors and demonstrates sensitivity to a combination of MEK and PI3K pathway inhibitors","volume":"8","author":"El","year":"2015","journal-title":"Dis. Model. Mech."}],"container-title":["Experimental Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014482717305128?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014482717305128?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:15:00Z","timestamp":1762146900000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014482717305128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["S0014482717305128"],"URL":"https:\/\/doi.org\/10.1016\/j.yexcr.2017.09.032","relation":{},"ISSN":["0014-4827"],"issn-type":[{"value":"0014-4827","type":"print"}],"subject":[],"published":{"date-parts":[[2017,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Culture methods of diffuse intrinsic pontine glioma cells determine response to targeted therapies","name":"articletitle","label":"Article Title"},{"value":"Experimental Cell Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.yexcr.2017.09.032","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}