{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:42:33Z","timestamp":1789875753187,"version":"4.0.1"},"reference-count":30,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T00:00:00Z","timestamp":1463616000000},"content-version":"am","delay-in-days":414,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1028794"],"award-info":[{"award-number":["OPP1028794"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental and Molecular Pathology"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1016\/j.yexmp.2015.01.011","type":"journal-article","created":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T00:52:55Z","timestamp":1421196775000},"page":"214-218","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["A multiplex PCR assay for the simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis"],"prefix":"10.1016","volume":"98","author":[{"given":"Ahmad N.","family":"Abou Tayoun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Burchard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Caliendo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Scherer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Tsongalis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.yexmp.2015.01.011_bb0070","first-page":"283","article-title":"Considerations on male infertility in genital infections with Chlamydia trachomatis (CT)","volume":"6","author":"Al-Moushaly","year":"2013","journal-title":"J. Med. Life"},{"issue":"8","key":"10.1016\/j.yexmp.2015.01.011_bb0165","first-page":"574","article-title":"A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene","volume":"5","author":"Anon.","year":"2006","journal-title":"J. Mol. Diagn."},{"issue":"2","key":"10.1016\/j.yexmp.2015.01.011_bb0160","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.mimet.2006.08.002","article-title":"Trichomonas vaginalis detection using real-time TaqMan PCR","volume":"68","author":"Anon.","year":"2007","journal-title":"J. Microbiol. Methods"},{"issue":"8","key":"10.1016\/j.yexmp.2015.01.011_bb0130","doi-asserted-by":"crossref","first-page":"2924","DOI":"10.1128\/JCM.39.8.2924-2927.2001","article-title":"Evaluation of laboratory testing methods for Chlamydia trachomatis infection in the era of nucleic acid amplification","volume":"39","author":"Battle","year":"2001","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"10.1016\/j.yexmp.2015.01.011_bb0075","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3928\/00904481-20130128-09","article-title":"Diagnosis and treatment of chlamydia, gonorrhea, and trichomonas in adolescents","volume":"42","author":"Beharry","year":"2013","journal-title":"Pediatr. Ann."},{"issue":"7","key":"10.1016\/j.yexmp.2015.01.011_bb0050","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1097\/OLQ.0b013e31816ddb9a","article-title":"Cost-effectiveness of screening strategies for Chlamydia trachomatis using cervical swabs, urine, and self-obtained vaginal swabs in a sexually transmitted disease clinic setting","volume":"35","author":"Blake","year":"2008","journal-title":"Sex. Transm. Dis."},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0125","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1083-3188(01)00137-1","article-title":"Use of ligase chain reaction for laboratory identification of Chlamydia trachomatis and Neisseria gonorrhoeae in adolescent women","volume":"15","author":"Braverman","year":"2002","journal-title":"J. Pediatr. Adolesc. Gynecol."},{"key":"10.1016\/j.yexmp.2015.01.011_bb0140","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/10647440500068248","article-title":"Real-time PCR provides improved detection of Trichomonas vaginalis infection compared to culture using self-collected vaginal swabs","volume":"13","author":"Caliendo","year":"2005","journal-title":"Infect. Dis. Obstet. Gynecol."},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0020","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1363\/3601104","article-title":"The estimated direct medical cost of sexually transmitted diseases among American youth, 2000","volume":"36","author":"Chesson","year":"2004","journal-title":"Perspect. Sex. Reprod. Health"},{"issue":"12","key":"10.1016\/j.yexmp.2015.01.011_bb0095","doi-asserted-by":"crossref","first-page":"e1134","DOI":"10.1016\/j.ijid.2013.07.011","article-title":"Performance of Anyplex\u2122 II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods","volume":"17","author":"Choe","year":"2013","journal-title":"Int. J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0040","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1097\/OGX.0b013e318279fb7d","article-title":"Trichomonas vaginalis in obstetrics and gynecology practice: new concepts and controversies","volume":"68","author":"Coleman","year":"2013","journal-title":"Obstet. Gynecol. Surv."},{"issue":"2","key":"10.1016\/j.yexmp.2015.01.011_bb0100","doi-asserted-by":"crossref","first-page":"89","DOI":"10.7326\/0003-4819-147-2-200707170-00007","article-title":"Gonorrhea and chlamydia in the United States among persons 14 to 39years of age, 1999 to 2002","volume":"147","author":"Datta","year":"2007","journal-title":"Ann. Intern. Med."},{"issue":"5","key":"10.1016\/j.yexmp.2015.01.011_bb0025","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/S1054-139X(03)00335-5","article-title":"A descriptive analysis of STD prevalence among urban pregnant African American teens: data from a pilot study","volume":"34","author":"Diclemente","year":"2004","journal-title":"J. Adolesc. Health"},{"issue":"4","key":"10.1016\/j.yexmp.2015.01.011_bb0090","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.diagmicrobio.2011.08.021","article-title":"Multiplex isothermal helicase-dependent amplification assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae","volume":"71","author":"Doseeva","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0115","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.19.1.165-256.2006","article-title":"Real-time PCR in clinical microbiology: applications for routine laboratory testing","volume":"19","author":"Espy","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"issue":"8","key":"10.1016\/j.yexmp.2015.01.011_bb0105","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1128\/JCM.00748-12","article-title":"Prevalence of Trichomonas vaginalis and coinfection with Chlamydia trachomatis and Neisseria gonorrhoeae in the United States as determined by the APTIMA Trichomonas vaginalis nucleic acid amplification assay","volume":"50","author":"Ginocchio","year":"2012","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.yexmp.2015.01.011_bb0060","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.ijgo.2013.09.013","article-title":"Sexually transmitted infections and women's sexual and reproductive health","volume":"123","author":"Gottlieb","year":"2013","journal-title":"Int. J. Gynecol. Obstet."},{"issue":"4","key":"10.1016\/j.yexmp.2015.01.011_bb0080","doi-asserted-by":"crossref","first-page":"e19263","DOI":"10.1371\/journal.pone.0019263","article-title":"What qualities are most important to making a point of care test desirable for clinicians and others offering sexually transmitted infection testing?","volume":"6","author":"Hsieh","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.yexmp.2015.01.011_bb0055","article-title":"Characteristics of sexually transmitted infections among high-risk HIV-positive patients attending an urban clinic in Uganda","author":"Katusiime","year":"2013","journal-title":"J. Int. Assoc. Provid. AIDS Care"},{"issue":"3","key":"10.1016\/j.yexmp.2015.01.011_bb0135","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1136\/sti.74.3.189","article-title":"Rapid assessment of sexually transmitted diseases in a sentinel population in Thailand: prevalence of chlamydial infection, gonorrhoea, and syphilis among pregnant women\u20141996","volume":"74","author":"Kilmarx","year":"1998","journal-title":"Sex. Transm. Infect."},{"issue":"11","key":"10.1016\/j.yexmp.2015.01.011_bb0045","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1128\/jcm.33.11.3049-3053.1995","article-title":"Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in genitourinary specimens","volume":"33","author":"Mahony","year":"1995","journal-title":"J. Clin. Microbiol."},{"issue":"5","key":"10.1016\/j.yexmp.2015.01.011_bb0035","doi-asserted-by":"crossref","first-page":"e64917","DOI":"10.1371\/journal.pone.0064917","article-title":"Intranasal vaccination with Chlamydia pneumonia induces cross-species immunity against genital Chlamydia muridarum challenge in mice","volume":"8","author":"Manam","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.yexmp.2015.01.011_bb0085","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/jcla.21485","article-title":"Evaluation of the new test VERSANT CT\/GC DNA 1.0 assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens","volume":"26","author":"Marangoni","year":"2012","journal-title":"J. Clin. Lab. Anal."},{"issue":"3","key":"10.1016\/j.yexmp.2015.01.011_bb0015","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1097\/OLQ.0b013e318285c6d2","article-title":"The estimated direct medical cost of selected sexually transmitted infections in the United States, 2008","volume":"40","author":"Owusu-Edusei","year":"2013","journal-title":"Sex. Transm. Dis."},{"key":"10.1016\/j.yexmp.2015.01.011_bb0120","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/978-1-62703-664-1_3","article-title":"Rapid methods for detection of MRSA in clinical specimens","volume":"1085","author":"Palavecino","year":"2014","journal-title":"Methods Mol. Biol."},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0150","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/0147-619X(86)90079-X","article-title":"A common plasmid of Chlamydia trachomatis","volume":"16","author":"Palmer","year":"1986","journal-title":"Plasmid"},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0065","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1186\/1471-2458-13-996","article-title":"Using intervention mapping for the development of a targeted secure web-based outreach strategy named SafeFriend, for Chlamydia trachomatis testing in young people at risk","volume":"13","author":"Theunissen","year":"2013","journal-title":"BMC Public Health"},{"issue":"1","key":"10.1016\/j.yexmp.2015.01.011_bb0145","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1097\/QCO.0b013e32834204d5","article-title":"The Swedish new variant of Chlamydia trachomatis","volume":"24","author":"Unemo","year":"2011","journal-title":"Curr. Opin. Infect. Dis."},{"issue":"7","key":"10.1016\/j.yexmp.2015.01.011_bb0110","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1097\/OLQ.0b013e3181cfcb34","article-title":"STD coinfections in The Netherlands: specific sexual networks at highest risk","volume":"37","author":"van Veen","year":"2010","journal-title":"Sex. Transm. Dis."},{"issue":"16","key":"10.1016\/j.yexmp.2015.01.011_bb0155","article-title":"Human Papillomaviruses and genital co-infections in gynaecological outpatients","volume":"9","author":"Verteramo","year":"2009","journal-title":"BMC Infect. Dis."}],"container-title":["Experimental and Molecular Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014480015000131?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014480015000131?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T02:42:03Z","timestamp":1757990523000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014480015000131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["S0014480015000131"],"URL":"https:\/\/doi.org\/10.1016\/j.yexmp.2015.01.011","relation":{},"ISSN":["0014-4800"],"issn-type":[{"value":"0014-4800","type":"print"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A multiplex PCR assay for the simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis","name":"articletitle","label":"Article Title"},{"value":"Experimental and Molecular Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.yexmp.2015.01.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}